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All Journal Jurnal Gizi dan Pangan Media Gizi dan Keluarga Nutri-Sains: Jurnal Gizi, Pangan dan Aplikasinya AcTion: Aceh Nutrition Journal Jurnal Gizi Klinik Indonesia Amerta Nutrition Jurnal Dunia Gizi Jurnal Ilmu Gizi dan Dietetik

Clara Meliyanti Kusharto

Departemen Gizi Masyarakat, Fakultas Ekologi Manusia (FEMA), Institut Pertanian Bogor, Bogor 16680

Author-ID : 302394

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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Teh putih (Camellia sinensis) dan kelor (Moringa oleifera) sebagai antihiperglikemia pada tikus Sprague dawley yang diinduksi streptozotocin Rahma, Amalia; Martini, Rina; Kusharto, Clara Meliyanti; Damayanthi, Evy; Rohdiana, Dadan
Jurnal Gizi dan Pangan Vol. 12 No. 3 (2017)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

This study aims to examine the antihyperglycemic effect of white tea (Camellia sinensis) and Moringa oleifera when administered as a single component and as mixture in streptozotocin-induced diabetic rats. The study design was experimental. The first step was to analyze epigallocathecingallat (EGCG) content as standard with High Performance Liquid Chromatography (HPLC) method. The second step was intervention in Sprague dawley rats induced by streptozotocin 40 mg/kgBW. Intervention held for 21 days with given white tea (WT), M. oleifera (MO), mixture of white tea and M. oleifera (WT+MO) and green tea (GT) as positive control with reference dose 100 mg/kgBW EGCG. Rats blood glucose and body weight were measured. The results are WT having the highest content of EGCG (4.46%). MO and WT+MO only have 2.42% and 2.46% of EGCG while GT as control is the lowest (1.29%). Measurements of blood glucose and body weight in group that given WT or MO as single component showed no significant difference with control negative group (p>0.05). Blood glucose decreased significantly in group WT+MO (p<0.05). Administration of WT+MO was able to maintain a stable body weight during the intervention period (p>0.05). The combination of white tea and M. oleifera has better ability as an antihiperglycemic agent compared to single component.

FORMULASI, KANDUNGAN GIZI, DAN DAYA TERIMA KUE-KUE TRADISIONAL MAKASSAR BERBASIS TEPUNG PUPAE–MULBERRY (PURY) SEBAGAI MAKANAN BERGIZI MASA DEPAN Kusharto, Clara Meliyanti; Astuti, Trina; Aisyah, Aisyah; Marliyati, Sri Anna; Rosmiati, Risti
Jurnal Gizi dan Pangan Vol. 10 No. 3 (2015)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

ABSTRACTThe aims of this research was to formulate and improve the nutrient content and define of makassar traditional snack which was enriched with Pupae-mulberry (Pury) powder. Experimental study was conducted and proximate analysis was used to determine nutrient content of the product and acceptance test was used by hedonic test of 25-32 semi-trained panelists. This research produced three kinds of traditional snacks, namely Rampari sponge cake, Deppatori Pury, and Pury sticks. Based on organoleptic test product Deppatori was selected to develop further by mixing basic substances consist of rice flour, glutinous rice flour, brown sugar, sesame and water with pupae-mulberry (pury) powder. The formulas were F1 (pury powder 5%), F2 (pury powder 10%), and F3 (pury powder 15%). The study showed that by hedonic scale, the panel preferred F2 because the addition more than 10% of Pury powder will make texture harder. Hedonic quality scale indicated that F2 had characteristics yellowish brown color, taste and flavor close to neutral. Nutrient content of Deppatori Pury 10% (per 100 g) was water 7.92 g, ash 1.19 g, protein 5.80 g, fat 25.99 g, carbohydrates 67.02 g, energy 484 kcal, calcium 74.14 mg, iron 1.96 mg, phosporus 97.23 mg, respectively and result of microbiology test was 390 cfu/g. Protein contribution of Deppatori-Pury 10% per 100 g serving size to RDA of elderly was 9.4-10.4%. Therefore, two serving size is recommended for Deppatori-Pury as future food to reach 20% RDA protein.Keywords: acceptability, deppatori, makassar’s snack, pury powderABSTRAKTujuan penelitian adalah melakukan formulasi dan meningkatkan kandungan zat gizi serta daya terima kue-kue tradisional Makassar yang diperkaya tepung pupae-mulberry (Pury). Penelitian ini merupakan studi eksperimental yang melakukan analisis kandungan zat gizi secara proksimat dan uji penerimaan panelis (organoleptik) menggunakan uji hedonik oleh 25-32 orang panelis semi terlatih. Pembuatan kue tradisional menghasilkan tiga jenis produk yaitu bolu-sponge Rampary, Deppatori-Pury, dan Pury-Stick. Berdasarkan hasil uji organoleptik, satu produk perlu dikembangkan lebih lanjut yaitu Deppatori yang memiliki tingkat kesukaan terendah. Formulasi Deppatori-Pury dilakukan dengan mencampurkan bahan dasar yang terdiri atas tepung beras, tepung ketan, tepung pury, gula merah, wijen, dan air. Formula Deppatori-Pury terdiri atas F1 (tepung pury 5%), F2 (tepung pury 10%), dan F3 (tepung pury 15%). Pada uji hedonik, panelis lebih menyukai F2 yakni formula dengan penambahan tepung pury sebesar 10%, karena penambahan tepung pury yang semakin banyak akan membuat tekstur semakin keras. Hasil uji mutu hedonik juga menunjukkan bahwa F2 memiliki karakteristik warna cokelat kekuningan, rasa dan aroma yang netral, serta tekstur yang padat agak keras. Kudapan Deppatori-Pury 10% per 100 g mengandung kadar air 7,92 g, abu 1,19 g, protein 5,80 g, lemak 25,99 g, karbohidrat 67,02 g, energi 484 kkal, kalsium 74,14 mg, besi 1,96 mg, fosfor 97,23 mg. Hasil uji mikrobiologis 390 cfu/g. Kontribusi protein Deppatori-Pury 10% dengan takaran saji 100 g terhadap AKG untuk kelompok lansia sebesar 9,4-10,4%, sehingga untuk mencapai kadar protein 20% dari AKG dianjurkan untuk dikonsumsi sebanyak dua kali takaran saji sebagai makanan masa depan.Kata kunci: daya terima, deppatori-pury, kue makassar, tepung pury

Nutritional Status, Physical Activity, Oxidative Stress, and Cognitive Function in Pre Elderly and Elderly Dainy, Nunung Cipta; Kusharto, Clara Meliyanti; Madanijah, Siti; Nasrun, Martina Wiwie Setiawan; Turana, Yuda
Jurnal Gizi dan Pangan Vol. 13 No. 3 (2018)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

This study aimed to analyse the relation of nutritional status, physical activity or oxidative stress with cognitive function of pre-elderly and elderly. A comparative cross-sectional study was conducted on 40 pre-elderly and 35 elderly subjects who were admitted to the Integrated Development Post Program (Posbindu) between September 2014 and January 2015. Anthropometric measurements (weight and height), physical activity, and biochemical data (oxidized low-density lipoprotein[Ox-LDL] and serum malondialdehyd [MDA]) were obtained. Nutritional status was assessed through body mass index (BMI), while the cognitive function was measured by Rey Osterrieth Complex Figure (ROCF) and Digit Span Backward. The data were analysed using an independent t-test and Spearman’s test with a confidence level of 95%. The study showed that the prevalence of malnutrition among the pre-elderly and elderly were 60.0% and 80.0%, respectively. Concerning physical activity, those who belonged to inactive-sedentary activity based on physical activity level (PAL) ratio were 55.0% and 45.7%, respectively. There were no significant differences in Ox-LDL, MDA levels and cognitive function (p>0.05). Significant negative correlations existed between Ox-LDL and immediate visual memory function (r=-0.289; p<0.05), as well as between Ox-LDL and delayed visual memory function (r=-0.288; p<0.05). The conclusion that visual memory function was only correlated with Ox-LDL.

Konsumsi minyak ikan lele (Clarias gariepinus) yang diperkaya omega 3 memperbaiki low density lipoprotein (LDL) dan kolesterol total pada lansia Srimiati, Mia; Kusharto, Clara Meliyanti; Tanziha, Ikeu; Suseno, Sugeng Heri
Jurnal Gizi dan Pangan Vol. 12 No. 2 (2017)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

The objective of this study was to analyze the effect of catfish oil supplementation on the LDL and total cholesterol levels in the elderly. Experimental design with randomized controlled trial (RCT) was used in this study. Subjects were divided into two groups, the first group (A1) was given catfish oil 1,000 mg per day, while the second group (A2) was not given the oil, it was called a control. The lipid profile measurement was performed before and after the intervention. Catfish oil consumed daily by treatment group for 60 days and then the effect on the lipid profile was evaluated. Total cholesterol and LDL in the two groups did not differ significantly. However, total cholesterol and LDL cholesterol of the treatment group had lower scores than the control group. In addition, the LDL levels in the treatment group were below maximum level, or maybe normal. The LDL and total cholesterol in the control group increased significantly (p<0.05), while in the treatment group was not significant. It might be due to enhancement of the consumption of the subject for 60 days. Thus, catfish oil enriched with omega 3 could inhibit the rate of enhancement of the LDL and total cholesterol.

Pemberian minyak ikan lele (Clarias gariepinus) terhadap bobot badan dan perubahan histopatologi hati, ginjal, dan otak tikus galur Sprague dawley yang diberi pakan hiperkolesterolemia Firmansyah, Hardi; Roosita, Katrin; Kusharto, Clara Meliyanti; Handharyani, Ekowati
Jurnal Gizi dan Pangan Vol. 12 No. 2 (2017)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

The objective of this study was to analyze changes in body weight and cell morphology in liver, kidney and brain tissue of Sprague dawley rats administered hypercholesterolemia diets for four weeks. Four diet treatments were applied in this study: control, catfish oil, catfish oil with the addition of omega 3 and catfish oil with the addition of omega 3 and vitamin E. Four weeks after the intervention, the rats were sacrificed and the internal organs (liver, brain, and kidney) were taken for histological preparations by hematoxylin-eosin (HE) and immunohistochemistry test. The study showed that the catfish oil intervention tend to reduced weight gain of rats fed hypercholesterolemia diets. More lipid accumulation in liver of the control group was found than the intervention groups. However, there was no difference in the histopathology of the kidney and brain tissues between the control group and intervention groups. It is concluded that catfish oil study provides a positive effect in rat liver tissue, but no difference exists between the brain and kidney tissue. There is no defect found at hippocampus of rats by formed of amyloid plague.

The Potential of White Tea (Camellia sinensis) and Kelor (Moringa oleifera) in Improving Lipid Profile and Histopathological Features of Pancreas in Streptozotocin-Induced Rats Martini, Rina; Rahma, Amalia; Kusharto, Clara Meliyanti; Riyadi, Hadi; Sumantri, Cece; Rohdiana, Dadan
Jurnal Gizi dan Pangan Vol. 14 No. 1 (2019)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

This study aimed to examine the potential of white tea and kelor in improving lipid profile and histopathological features of the pancreas in streptozotocin-induced Sprague-Dawley rats. The research design was an experimental study with post-test control group design. Catechin content were analysed using High-Performance Liquid Chromatography. Twenty Sprague-Dawley rats (out of 24 rats, 12-week old, 200-300 g) were induced with intraperitoneal injection of streptozotocin at a dose of 40 mg/kg BW. Four groups of rats received one of the green tea (GT), white tea (WT), kelor (K), or the mixture of white tea and kelor (WTK) with a dose of epigallocatechin-3-gallate (EGCG) at 100 mg/kg BW administered orally for 21 days. Measurements of the lipid profile and preparation of histopathological features of the pancreas were performed after the intervention was completed. The results showed that white tea had the highest concentrations of catechin (39.17%), gallic acid (1.09%), EGCG (4.46%), and epicatechin (9.61%) compared to the other tea groups. The TG levels of the WTK group (95±19.35 mg/dl) were significantly different (p<0.05) from the WT and K groups but not significantly different from the normal (105.8±23.89 mg/dl), DM and GT groups. Meanwhile, the HDL levels did not show significant differences in each intervention group (p>0.05). The mean diameter of the islets of Langerhans in the DM group (9.16± 2.56 μm) was significantly different (p<0.05) from the WT (20±8.94 μm), K (17.16±5.26 μm), WTK (18.66±4.17 μm), and N (21.07±8.49 μm) groups but not significantly different from the GT group (14.33±5.24 μm). The histopathological features of the pancreas showed an increase in the diameter of the islets of Langerhans in WT, K, and WTK groups. This study revealed that the mixture of white tea and kelor had the potential to ameliorate triglyceride levels and histopathological features of the pancreas in streptozotocin-induced Sprague-Dawley rat.

High Protein Milk Intervention in Malnourished Children and Its Effect on Weight Gain and Renal Function Kusharto, Clara Meliyanti; Ekawidyani, Karina Rahmadia; Tanziha, Ikeu; Setiawan, Budi; Rosmiati, Risti; Irawan, Andi Muh Asrul
Jurnal Gizi dan Pangan Vol. 14 No. 1 (2019)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

This study aimed to determine the effect of high-protein milk intervention on weight gain and renal function in malnourished children. This research is a randomized controlled trial (RCT). A total of 52 wasting children (BAZ -3<SD<-2) aged 3-12 years were selected by simple random sampling from five primary schools (6-12 years) and two Community Health Center (children below 6 years). Subjects were divided into two groups; control group (26) and intervention group (26). The intervention group was given high-protein milk (HPM) 3x25 g each day for 12 weeks. Body weight, serum creatinine, and urea were measured at week 0 (baseline), 6th (midline), and 12th (endline). The study showed that the body weight of the intervention group significantly increased by 1.54 kg after 12 weeks of high-protein milk consumption. Serum urea and creatinine levels in the intervention group also significantly increased (p<0.05). The increase in serum urea and creatinine levels in the intervention group were still within the normal range (normal serum urea level 15-43 mg/dl and normal creatinine level 0.6-1.2 mg/dl). This suggests that significant increases in urea and creatinine levels of the intervention group were normal because of increased protein intake and body muscle mass and it did not lead to renal function abnormalities.

Co-Authors Adi Teruna Effendi Adi Teruna Effendi Ai Kustiani Aisyah Aisyah Ali Khomsan Amalia Rahma Anggun Rusyantia Anna Fatchiya Arum Atmawikarta Ascobat, Purwantyastuti Astuti Lamid Budi Setiawan Budi Setiawan Budi Setiawan Cece Sumantri Dadan Rohdiana Dadang Sukandar Darningsih, Sri Dewi Ratih Agungpriyono Ekawati S. Wahyuni Ekowati Handharyani Ellis Endang Nikmawati Evy Damayanthi Faisal Anwar Fauziah Fauziah Firda Amalia Firmansyah, Hardi Hadi Pratomo Hadi Riyadi Hardi Firmansyah Harna, Harna Ikeu Tanziha Karina Rahmadia Ekawidyani Katrin Roosita M. Husni Thamrin M. Januwati Martina Wiwie Setiawan Nasrun Mia Srimiati Muh Asrul Irawan, Andi Muhilal Muhilal Ni Ketut Sutiari Nisa, Ayu Rizkia Nunung Cipta Dainy Nurdin Nurdin Nurdin Nurdin Prita Dhyani Swamilaksita Purwantyastuti Ascobat Rimbawan , Rina Martini Risti Rosmiati Rohayati Suprihatini Rusilanti Rusilanti Sa'pang, Mertien Siti Madanijah Sri Anna Marliyati Sugeng Heri Suseno Trina Astuti Trina Astuti Yuda Turana

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