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Journal : JURNAL ATOMIK

UJI AKTIVITAS ANTIHIPERGLIKEMIK EKSTRAK ETANOL DAUN TEMBELEKAN (Lantana camara L.) TERHADAP MENCIT PUTIH JANTAN TERINDUKSI ALOKSAN Eka Setia Rini; Rudi Kartika; Rahmat Gunawan
JURNAL ATOMIK Vol 1 No 2 (2016)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Phytochemical screening has been conducted on the ethanol extract of tembelekan leaves (Lantana camara Linn) which showed the presence of alkaloids, flavanoids, steroids, triterpenoids and phenolic. Testing antihyperglicemic activity of ethanol extract tembelekan leaves conducted experiments in the laboratory with each dose 14 mg/kg BW, 28 mg/kg BW and 56 mg/kg BW given orally in male mice induced by alloxan. Measurement of blood glucose levels done on day 0, 4, 8, 14 and 18 by using glucometer and glucose test strips. The decrease in blood glucose levels is 56,89 % with ethanol extract tembelekan leaves dose 56 mg/kg BW. The results showed that there is decrease in blood glucose because given ethanol extract tembelekan leaves.
Pengembangan Metode Rapid Test Preparation Dalam Penentuan Kadar Inherent Moisture dan Total Sulfur Dengan Menggunakan Metode yang Dipergunakan oleh ISO (International Organization for Standardization)) Lumban Gaol Veronica Destiana; Aman Sentosa Panggabean; Rudi Kartika
JURNAL ATOMIK Vol 2 No 1 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The research about the development of rapid test method in determination of inherent moisture concentration and total sulfur which used by the ISO (International Organization for Standardization) has been diving. This research aims to determine is the rapid test method could be used to analyze inherent moisture and total sulfur to determine the analytical performance of the rapid test. Parameters of the validation used in this research are accuracy which described in percent of recovery as the percent of the recovery of inherent moisture are 99,98 % and total sulfur value are 98,57 %, precision described in coefficient of the variant which gained in inherent moisture are 0,49% and total sulfur value is 4,1%. Robustness of method is described in the variation of drying time, which accuracy and precision do not affect by time variation in preparation. The results show that rapid test method can be used for an alternative method which used by the ISO.
STUDI BIOAKUMULASI ION LOGAM Pb DALAM RAMBUT DAN DARAH OPERATOR STASIUN PENGISIAN BAHAN BAKAR UMUM, JALAN SENTOSA, SAMARINDA Sukma Wiratama; Saibun Sitorus; Rudi Kartika
JURNAL ATOMIK Vol 3 No 1 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

A study about Bioaccumulation of Pb Metal Ion in Hair and Blood of Operator of General Fuel Filling Station, Sentosa Road Samarinda has been conducted. The aim of the study is to determine the accumulation of Pb metal ions levels and the association between the hours of work of gas station operator assumed to be positively correlated and the levels of Pb metal ions in hair as well as in blood. Hair and blood were homogenized and analyzed by Atomic Adsorption Spectrophotometer (AAS). The results displayed that the lowest and highest level of Pb metal ions contained in hair was 0.03 μg/g and 0.07 μg/g, respectively. The highest number found in blood was similar to the level contained in hair, that is 0.07 μg/mL. However, the lowest level was marginally lower with a number 0.01 μg/mL. The coefficient correlation (r) obtained were 0.822 and 0.859 for hair and blood, respectively. These numbers indicate there was a strong positive relationship between working hours of gas station operator and levels of Pb metal ion in hair and blood. This inference was also supported by the results of t test, that is, 0.564. Furthermore,% determinants analysis was also undertaken which result in 82.2% and 85.9% for hair and blood, respectively to strengthen the test result that working hours of gas station operator in Sentosa Road Samarinda was positively associated with Pb metal ion levels in hair and in blood.
PEMBUATAN ETANOL DARI KULIT PISANG MAHULI (Musa paradisiaca. var sapientum) Suaibatul Aslamiah; Bohari Yusuf; Rudi Kartika
JURNAL ATOMIK Vol 1 No 2 (2016)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Pembuatan etanol dari kulit pisang mahuli (Musa paradisiaca. var sapientum) telah dilakukan. Metode yang digunakan dalam penelitian ini adalah hidrolisis enzimatis yaitu mengubah pati menjadi glukosa dengan bantuan enzim α-amilase dan gluko-amilase, kemudian dilanjutkan dengan penambahan nutrisi urea serta proses fermentasi alkohol dan destilasi. Hasil fermentasi kemudian dianalisa menggunakan Kromatografi Gas. Konsentrasi etanol yang optimum didapatkan pada fermentasi hari keempat sebesar 6,212% dengan konsentrasi urea optimum yaitu 0,5%.
PEMBUATAN BIOETANOL DARI SINGKONG KARET (Manihot Glaziovii Muell) DENGAN HIDROLISIS ENZIMATIK DAN DIFERMENTASI MENGGUNAKAN Saccharomyces Cerevisiae Arifwan -; Erwin -; Rudi Kartika
JURNAL ATOMIK Vol 1 No 1 (2016)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Singkong Karet (Manihot glaziovii Muell) merupakan umbi yang tidak termasuk bahan makanan  karena mengandung  unsur kimia asam sianida (HCN) yang bersifat racun. Kandungan pati dalam ubi mencapai 98,5 %. Penelitian ini bertujuan untuk mengetahui konsentrasi amonium sulfat dan waktu fermentasi terbaik terhadap kadar bioetanol yang dihasilkan. Proses hidrolisisi pati dilakukan secara  enzimatis melalui tahap liquifikasi dan sakarifikasi dengan menggunakan enzim alfa-amilase dan gluko-amilase yang akan mengkonversi pati menjadi glukosa. Glukosa hasil hidrolisis ditambahkan amonium sulfat sebagai sumber nutrisi dengan variasi 0,75 %; 1 % dan 1,25 % (b/v) kemudian difermentasi menggunakan jamur Saccharomyces cereviceaea dengan variasi waktu 120, 144 dan 168 jam menjadi bioetanol. Pemurnian bioetanol dilakukan dengan proses destilasi. Analisa kadar alkohol menggunakan kromatografi gas. Hasil penelitian menunjukkan penambahan nutrisi amonium sulfat yang paling optimum adalah 1 % (b/v) dan waktu fermentasi yang optimum adalah 168 jam dengan kadar etanol sebesar 28,183 %..Kata-kata kunci:  Singkong karet, fermentasi, amonium sulftat, bioetanol
PHYTOCHEMICAL TEST AND STABILITY TEST OF PIGMENT OF KETAPANG LEAF (Terminalia catappa Linn.) Sulistiyawati, Rizki Ramadhani; Saleh, Chairul; Kartika, Rudi
JURNAL ATOMIK Vol 6 No 2 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Extraction of ketapang leaf (Terminalia catappa Linn) was carried out by maceration method using 96% ethanol. Phytochemical test results showed that the ketapang leaf extract contained tannin compounds. Stability test of ketapang leaf extract was carried out on the influence of temperature 40, 60 and 800C, the effect of pH (3, 4 and 5) and the effect of storage duration 1, 3 and 5 days using wavelength UV. The results of this study indicate that the ketapang leaf dye is stable at pH 3-4, stable decrease by heating at temperature 40-800C and stable at storage for 1-3 days.
CORRELATION OF Pb METAL ION LEVELS ON PROTEIN CONCENTRATION IN CRUSH CONSTRUCTION (Gafrarium tumidium) TAKEN IN THE COAST OF BONTANG, EAST KALIMANTAN Corebima, Damianus Develistur; Kartika, Rudi; Hindryawati, Noor
JURNAL ATOMIK Vol 5 No 2 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Research on the correlation of Pb metal ion levels to protein content in crush clams (Gafrarium tumidium) taken at the coast of the East Kalimantan Rhino Estuary has been conducted. This study aims to determine the levels of Pb metal ions and protein levels in blood clams based on the size and location of different samples. Correlation of Pb metal ion levels and protein in blood clams was tested using the Least Square method and Pearson product moment. Analysis of Pb metal ion levels using SSA (Atomic Absorption Spectrophotometer) and protein content analysis using the Kjeldahl method. In this study, the Pb metal ion level was obtained at the public port of Tanjung Limau, PPI Tanjung Limau and beach Lallo Sekangat with an average of 0.7474 mg/L, 0.5513 mg/L, and 0.2804 mg/L respectively. Crush clam protein content at Tanjung Limau, PPI Tanjung Limau and beach Lallo Sekangat with an average of 19.6544%, 14.6902%, and 10.9135% respectively. The correlation of Pb metal levels to protein levels in crush clams (Gafrarium tumidium) was stated positively with coefficient correlation results was strong (r = 0.7910).
ENDOPHYTIC BACTERIA SCREENING FROM THE LEAVES OF OKRA (Abelmoschus esculentus L. Moench) WHICH PRODUCED LIPASE Utami, Nanda Putri; Astuti, Winni; Kartika, Rudi
JURNAL ATOMIK Vol 5 No 2 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Endophytic bacteria found in plant tissues can produce enzymes, one of which is lipase. The purpose of this study was to screening endophytic bacteria from the leaves of Okra (Abelmoschus esculentus L. Moench) which produced lipases. The method used in this study is screening with media nutrient agar (NA) containing Rhodamine B and olive oil. The results showed that 4 of bacterial isolates (D1, D2, D3 and D4) from 14 endophytic bacterial isolates had lipase activity. The crude lipase extract was quantitatively tested for lipase activity by the titrimetry method. The highest lipase activity was shown by isolates with a D1 code of 1.50 U/mL.
PEMBUATAN ETANOL DARI UMBI TALAS (Colocasia esculenta [L] Schott) DENGAN PENAMBAHAN GELATIN SEBAGAI SUMBER NITROGEN Saccharomyces Cerevisiae Abimanyu, Priem Reyhan; Kartika, Rudi; Sitorus, Saibun
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Ethanol have made from taro’s flour with an addition of gelatin as nutrition source for Saccharomyces cereviciae. Ethanol are made within process hydrolysis, fermentation, distillation and Gas Chromatography analysis. Starch hydrolysis process are enzymatic through liquification and saccharification process with an addition of alpha-amylase and gluco-amylase which conversed starch into glucose. Fermentation process are used time variation within 5, 6, 7 and 8 days and gelatin as nutrition source are used in concentration to 0,5 %, 1 % and 1,5 % (v/v). The highest ethanol concentration are obtained in addition of 1 % gelatin with 7 days of fermentation which are 41,648 %.
SELEKSI BAKTERI POTENSIAL PENGHASIL ENZIM HIDROLITIK DARI SAMPEL TANAH TEMPAT PEMBUANGAN AKHIR BUKIT PINANG SAMARINDA Admaja, Junior Try; Astuti, Winni; Kartika, Rudi
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1401

Abstract

Bacteria are one of the microorganisms that have the potential to produce hydrolytic enzymes. This research was carried out to isolate bacteria from the soil of the Bukit Pinang Samarinda Final Disposal Site and select bacteria that produce cellulase, protease and lipase enzymes. Bacteria from landfill soil were isolated using the spread plate method and 10 single colony bacterial isolates were selected which were then coded TPA 1 – TPA 10. The results of the selection of bacteria producing hydrolase enzymes showed that 9 isolates were capable of producing protease enzymes, 6 isolates were capable of producing cellulase enzymes and 4 isolates were capable of producing cellulase enzymes. The isolate was able to produce the lipase enzyme.
Co-Authors Abimanyu, Priem Reyhan Adha, Laila Sufina Aditya, Roy Admaja, Junior Try Adnyana, Ketut Cahyadi Ahmad Suryadi, Ahmad Alimuddin - Alimuddin - Aman S Panggabean Aman S Panggabean, Aman S Aman S. Panggabean Aman Sentosa Panggabean Andi Kurniawan Annuary Laksono Arifwan - Ary Rizki Darmawi Ary Rizki Darmawi, Ary Rizki Aswad, Muhammad Raihan Aswat, Muhammad Raihan Aswat, Raihan Azka, Andi Aulia Urrahman Bayu Iskandar Bohari Yusuf Bohari Yusuf Bonifasius, Bonifasius Bustanussalam Bustanussalam Bustanussalam Bustanussalam, Bustanussalam Chairul Saleh Chairul Saleh Corebima, Damianus Develistur Delina Khairunnisa Dilo Jhosea Luciano Eduardo Dimas P, Riansyah Djihan Ryn Pratiwi, Djihan Ryn Dwi Lestari Dwi Susanto Edi Novian Jaya Edman Tandirerung Eka Damayanti Setyningrum Eka Setia Rini Eka Siswanto Syamsul Eno Julia Shafarini Erwin - Erwin Erwin Eva Marliana, Eva Fahmi, Miraz’hul Fahmi, Miraz’ul Farida Farida Fathur Rahman Fathur Rahman fatur Rahman, fatur Fauzi Rachman Fauzi Rachman Firmansyah - Fitriana, Mila Gelung, Antonius Hamsiana - Hendra Priatna Hendric Surya Putra I Wayan Arief Pradana Putra Iskandar, Bayu Jaya, Edi Novian Jofri, Dirga Hosea Seventeen Jofri, Dirga Hosea Seventin Julianto, Faisal Reja Kalmud Nia Tekha Laksono, Annuary Lila Karina Nuryanti Lilik Sulastri Lumban Gaol Veronica Destiana Mahrus, Muhammad Haissul Maria Amelia Kristianingrum Marliani, Nelly Marlina, Eva Mila Fitriana Mira Wahyuni Mira Wahyuni, Mira Miraz’hul Fahmi Muhammad Adhitya Rizkirullah Muhammad Haissul Mahrus Muhammad Harry Rahmadan Muhammad Raihan Aswat Muhammad Sigit Ariski Nasution, Safridah Hannum Noor Hindryawati Noor Hindryawati, Noor Nur Asmawati Nur, Istianti Irfa'a Octavianus, Riyan Octavianus, Riyan Partomouan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Prastiwi, Mela Pratama, Riansyah Dimas Rahmat Gunawan Rahmat Gunawan Rahmat Gunawan Rezky Estrada Ricard, Romoldus Jonius Rico Ramadhan, Rico Rida Awliyani Ripaldy Apriansya Riyan Octavianus Rizkirullah, M. Adhitya Rizkirullah, Muhammad Adhitya Rizkirullah, Muhammad Aditya Rizkullah, Muhammad Adhitya rizky maulida amalia hanif Ruga, Ritbey Rutdianti Rutdianti Rutdianti, Rutdianti Sahriani, Andi Saibun Sitorus Sanian, Sanian Sari Setianingsih Setianingsih, Sari Setyningrum, Eka Damayanti Shafarini, Eno Julia Shaparuddin Nur Arifullah Simanjuntak, Partomuan Solman, Solman Suaibatul Aslamiah Subur P Pasaribu Sudrajat Sudrajat Sukanadi, I Putu Sukma Wiratama Sulistiani, Oktaviana Dewi Sulistiyawati, Rizki Ramadhani Syelvia Lousiana Kalauw Syelvia Lousiana Ningsih Kalauw Tandirerung, Edman Tanjung, Faisal Trisnawati, Eka Elly Ulfa, Yulyanti Sartika Utami, Nanda Putri Wahyu Budi Utama Wahyu Budi Utama Wahyu Teguh Santoso Wahyu Teguh Santoso Wajdie, Farid Winni Astuti Winni Astuti, Winni Wulan Verani Yeni Agustina Yeni Agustina, Yeni Yerwanto Ilang Yerwanto Ilang, Yerwanto