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All Journal Buletin Udayana Mengabdi E-Jurnal Medika Udayana Health Science Journal of Indonesia E-Jurnal Udayana Medica The Indonesian Biomedical Journal Indonesian Journal of Cardiology Indonesian Journal of Medicine ISM (Intisari Sains Medis) : Jurnal Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Archives of The Medicine and Case Reports Journal of Global Pharma Technology
Anak Agung Wiradewi Lestari
Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Journal of Global Pharma Technology

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Concordance and Validity of Urinalysis Using Sysmex UX-2000, Cobas U411 and Manual Microscopic Examination Anak Agung Wiradewi Lestari
Journal of Global Pharma Technology Volume 10 Issue 12.
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Objective: Urinalysis is important in screening, diagnosing, and monitoring the progress of certain diseases. However, the procedure takes time to finish, special skill, and lack of standardization in certain labs. This study evaluates the concordance between manual microscopic examination results to Sysmex UX-2000, and urinalysis chemical results between Sysmex UX-2000 and Cobas U411 (Roche Diagnostic), and the validity of urine sediment results between Sysmex UX-2000 and manual microscopic examination.  Methods: Eighty urine samples were analyzed using Sysmex UX-2000, Cobas u411, and manual microscopic examination. Concordance level was measured using kappa coefficient. Then we calculated the validity of Sysmex UX-2000 in sediment tests to manual microscopic examination. Results: Chemical urinalysis of Sysmex UX-2000 possesses good and very good concordance level to Cobas u411 (*weighted κ 0.65-0.91). Urine sediment tests of Sysmes UX-2000 possesses good concordance to manual microscopic examination (**weighted κ 0.76-0.77), with 86-100% sensitivity and 73-78% specificity. Conclusion: Sysmex UX-2000 has good concordance level to Cobas u411 for chemical tests urinalysis, and also good concordance and validity between Sysmex UX-2000 to microscopic examination for erythrocyte and leucocyte sediment tests.Keywords: Analytical System, Renal Diseases, Quality Assurance and Control.
The Use of Methanolic Extract of Annona Muricata Linn Leaves as an Effective Anti-Hyperglycemia on Mice Anak Agung Wiradewi Lestari
Journal of Global Pharma Technology Volume 11 Issue 08 (2019) Aug. 2019
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Background: Extracts from various morphological parts of soursop leaf (Annona muricata Linn/Annonaceae) are widely used in many parts of the world for the management, control, and treatment of a human diseases, including diabetes mellitus (DM).  This study investigates possible anti-hyperglycemia effects of methanolic extract of Annona muricata Linn in mice experimental paradigms of DM.Materials and Methods: The mice used were divided into three experimental groups. Group A served as ‘control’ animals and received sodium citrate buffer solution in quantities equivalent to the administered volumes of methanolic extract of Annona muricata Linn. Diabetes mellitus was induced in Groups B and C by intraperitoneal injections of streptozotocin (STZ, 80 mg/kg body weight). Group C were additionally treated with methanolic extract of Annona muricata Linn (100 mg/kg body weight, PO) for two weeks.Results: A statistically significant lower blood glucose level was found in Group C compared to Group B in sixth (215.14±78.37 vs 319.57±135.92 mg/dL) and seventh week (158.57±24.23 vs 238.85±51.35 mg/dL) of observation.Conclusion: Daily dose of 100 mg/kg body weight methanolic extract of Annona muricata Linn administration lowers blood glucose compared to those who did not receive in mice.
Difference in Sodium and Potassium Reading by Blood Gas Analyzer and Electrolyte Analyzer at Sanglah Hospital Denpasar, Bali, Indonesia Anak Agung Wiradewi Lestari; I Putu Sidhi Rastu Karyana; I Nyoman Wande
Journal of Global Pharma Technology Volume 10 Issue 07: (2018) July 2018
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Introduction: Electrolytes measurement is a standard procedure in medical practice to diagnose diseases or disease-related complication. Recently, there is a various device used to analyze blood electrolytes, but the difference accuracy between devices is a problematic issue for clinicians. Thus, we evaluate the differences of sodium and potassium concentration reading by Blood Gas Analyzer (BGA) and Electrolyte Analyzer at Sanglah Hospital to assess whether the difference could be accepted according to Clinical Laboratories Improvement Amendments (CLIA). Methods: A cross-sectional analytic study was conducted in May 2017 at Clinical Pathology Laboratory of  Sanglah Hospital, Denpasar. 30 subjects were enrolled consecutively during the study period and the blood samples were analyzed by both BGA and electrolyte analyzer. The data were analyzed using paired T-Test, Bland-Altman Plot, and Linear Regression. Result: The mean of sodium and potassium measured by BGA were 133.76 ± 5.68 mmol/L and 3.14 ± 0,86 mmol/L, respectively while electrolyte analyzer was 136,43 ± 6,48 mmol/L and 3,51 ± 0,9 mmol/L, respectively. Mean difference between sodium and potassium were 2.67 mmol/L (P=0,000) and 0.36 mmol/L (P=0,000), respectively. Bland-Altman plot analysis in sodium and potassium showed that the limit of the agreement was at -9.9 to 4.56 mmol/L and -1.28 to 0.56 mmol/L, respectively. Conclusion: There was a significant difference between BGA and electrolyte analyzer reading with BGA has higher acceptance level compared to electrolyte analyzer.Keywords: Natrium, Kalium, Blood Gas Analyzer, Electrolyte Analyzer.
The Diagnostic Value of Hba1c Turbidimetry Immunoassay in Sanglah General Hospital Denpasar, Bali, Indonesia Anak Agung Wiradewi Lestari
Journal of Global Pharma Technology Volume 10 Issue 07: (2018) July 2018
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Introduction: Diabetes mellitus (DM) is a common health problem in Indonesia. Currently, Hemoglobin A1c (HbA1c) is the gold standard to diagnose and monitor DM patient. HbA1c could be assessed using turbidimetry immunoassay and High-Performance Liquid Chromatography (HPLC). However, the comparison between the two devices is never conducted. Thus, this study aimed to determine the sensitivity, specificity, and correlation of HbA1c measurements using turbidimetry immunoassay method compared to HPLC as the gold standard. Methods: A cross sectional study was conducted from May to June 2017to measure the diagnostic values and correlation test between turbidimetry immunoassay method and HPLC. Diagnostic values were measured as sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). Blood samples were collected in EDTA tubes and measured with turbidimetry immunoassay and HPLC, with a cut-off value of 6.5 mg/dL. Results: Fifty samples were collected during the study. HbA1c ≥6.5mg/dL were obtained from 27 samples, and normal results were obtained from 23 samples. Sensitivity, specificity, PPV, and NPV from turbidimetry immunoassay were 92.86%, 100%, 100%, and 91.67% respectively. Pearson correlation test of HbA1c result from turbidimetry immunoassay and HPLC revealed a strong positive correlation with r = 0,988. The ROC curve analysis yield AUC value of 0.926 with P=0.000 which indicate a strong diagnostic value.Conclusion: Turbidimetry immunoassay method had high sensitivity and specificity and had strong diagnostic value according to ROC analysis. There was also a strong positive correlation between turbidimetry immunoassay and HPLC HbA1c reading.Keywords: HbA1c, HPLC, Turbidimetry immunoassay.
Co-Authors Aakash Aakash Airin Que Anak Agung Ayu Lydia Prawita Anak Agung Ayu Yuli Gayatri Anak Agung Gde Bisma Sanjaya Anak Agung Ngurah Subawa Anak Agung Putri Satwika Aryana, I Gusti Ngurah Wien Aryati Aryati Bagus Anom Sudiada Bagus Ari Pradnyana Dwi Sutanegara Budi Santosa Budi Santosa Cong, Tzeto Han Desak Gde Diah Dharma Santhi Dewi, Ni Made Ratih Purnama Dharayani, Luh Anandita Diah Pradnya Paramita Edward Kurnia Setiawan Limijadi, Edward Kurnia Setiawan Eka Wiratnaya Ekarini Katharina Yunarti Nabu Ekarini Katharina Yunarti Nabu Endrawati KJ Felim, Jethro Gde Ary Putra Kamajaya Gede Wira Mahadita Giri, Kadek Gede Bakta Haneetha Yogarajah I A Putri Wirawati I Gde Raka Widiana I Gst Agung Dwi Mahasurya I Gusti Ngurah Wien Aryana I Gusti Putu Suka Aryana I Kadek Arya Candra I Ketut Suyasa I Nyoman Astika I Nyoman Hery Sumertayasa I Nyoman Indrawan Mataram I Nyoman Santa Wijaya I Nyoman Wande I Nyoman Wande I Putu Sidhi Rastu Karyana I Putu Yuda Prabawa I Wayan Agus Surya Pradnyana I Wayan Putu Sutirta Yasa I Wayan Suryanto Dusak I.A. Putri Wirawati Ida Ayu Ari Utami Ida Bagus Putu Putrawan Indrayani, Pande Kadek Putri Rahayu Intan Astariani Ketut Gede Mulyadi Ridia Ketut Siki Kawiyana Ketut Suega Komang Agus Widhy Utama Putra Komang Septian Sandiwidayat Krisna Yoga Pratama Made Ayu Widyaningsih Made Dyah Khrisnadewi Made Kris Budiman Made Wirabhawa Mahartini, Ni Nyoman Mardiyah Hayati Michael Ferdinand Milaviwanda, Luh Komang Ayu Nabu, Ekarini Katharina Yunarti Ni Gusti Ayu Putu Lestari Santika Dewi Ni Kadek Mulyantari Ni Ketut Rai Purnami Ni Komang Krisnawati Ni Luh Gede Yoni Komalasari Ni Luh Putu Pranena Sastri Ni Made Stithaprajna Pawestri Prabawa, I Putu Yuda Putu Astawa Putu Ayu Krisna Cahyaning Putri Putu Gde Hari Wangsa Putu Putri Titamia Saraswati Putu Ugi Sugandha R. A.T. Kuswardhani Sagung Novita Widyaningrat Sianny Herawati Sianny Herawati Sri Darmawati Sudana, I Nyoman Gde Sudewa Djelantik Teguh Triyono Thaha .. Tjok Gde Oka Tjokorda Gede Oka Triningrat, Anak Agung Mas Putrawati Usi Sukorini Vera Akmilia Wande IN Wayan Aryadana Wayan Giri Putra Wijaya Kusuma Wira Gotera Wirawati, Ida Ayu Putri Yenny Kandarini