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GAMBARAN DARAH MERAH IKAN PATIN SIAM (Pangasius hypopthalmus) YANG DIBERI PAKAN MENGANDUNG PROPOLIS Purwanto, Purwanto; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThis reseach was conducted in March-June 2018 in Fish Diasease and Parasite Laboratories and Experiment of the faculty of Fisheries and Marine Science University of Riau. The purpose of this study was to determine the effect of the description of red blood (total erythrocytes, hemoglobin, and hematocrit) and the growth of catfish (Pangasius hypopthalmus). The method used was experiment with Completely Randomized Design (RAL) with one factor, the level 5 treatments and 3 replications. The treatment in this study is, P0: control without given, P1 (addition of propolis in feed at a dose of 600 ppm/kg), P2 (700 ppm/kg), P3 (800 ppm/kg), P4 (900 ppm/kg). The parameters measured were total erythrocytes, hemoglobin levels, hematocrit values, absolute weight growth, fish survival, and water quality. The results showed that feeding containing propolis given for 60 days in catfish (Pangasius hypophthalmus) gave a significant effect on the number of erythrocytes, hemoglobin levels, hematocrit values, absolute weight growth with P <0.05 and not significantly different from fish survival P> 0.05. The best treatment at the dose of P3 (800 ppm) with total erythrocytes 276 x 104cells / mm3, hemoglobin levels of 10.6 g / dL, and hematocrit levels 33.3%, and absolute weight growth of 39.18 gr, and survival of 98 %. The results of measurements of water quality during the study were temperatures ranging from 27-310C, DO ranged from 3.4 to 3.9 ppm, pH ranged from 5.2 to 7, and NH3ranged from 0.017 to 0.024 ppm.Keywords: Propolis, Haematology, Pangasius hypopthalmus
THE EFFECTS OF GIVING Citrus hystrix SOLUTION on ERYTHROCYTES PROFILE of pangasius hypophthalmus Irawan, Chairani; Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTCitrus hystrix is an essential oil producing plant. In addition to essential oils, there are several ingredients of Citrus hystrix as an antibacterial agent, namely: vitamin C, flavonoids, tannins and alkaloids. This research was conducted on June until August 2019. The aims of this research was to get obtain information about the effect of giving a solution of Citrus hystrix to the erythrocyte image of conjoined Pangasius hypophthalmus and find out the best dosage for the prevention of MAS (Motile Aeromonas Septicaemia) disease, seen from the total erythrocytes, hemoglobin, hematocrit, and morphology of erythrocyte patchouli fish (Motile Aeromonas Septicaemia). The method used is an experimental method with a completely randomized design (CRD), one factor with 5 treatment and three raplication of KN: (without immersed with Citrus hystrix solution and without infected by  Aeromonas hydrophila) KP: (without immersed with Citrus hystrix solution and infected by Aeromonas hydrophila.) P1: (1200 ppm), P2: (1500 ppm), P3: (1800 ppm). immersed of Citrus hystrix solution was carried out four times, namely on days 7,14,21 and 28, soaking was carried out for 2 minutes. Then on the 31st day a challenge test was carried out with Aeromonas hydrophila bacteria with a density of 108 CFU / ml of 0.1 ml /fish. The results showed that immersed of fish with a solution of Citrus hystrix gave effect to the erythrocyte image of conjoined Pangasius hypophthalmus. The best dosage is P2 1500 ppm treatment seen from total erythsoit 172.67 x 104 cells/mm3, hematocrit value 31.33% and hemoglobin value 6.93 g/dl. Siamese Pangasius hypophthalmus life reaches 100%. The quality of water obtained during the study were temperatures ranging from 27.3 - 28 oC, pH 6-7, DO 3.2 - 4.7 mg/L and Ammonia 0.02 mg/L.Key word :Citrus hystrix, Catfish, Aeromonas hydrophila, immertion,erythrocyte,
HEMATOLOGICAL PROFILES OF AFRICAN CATFISH (Clarias gariepinus) THAT ARE MAINTAINED BY BIOFLOC SYSTEMS Kurniawan, Ahmad Juli; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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                                              ABSTRACTThe researcher conducted this research from November to December 2018 in two places: Ambon Farm Pekanbaru and the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine Sciences, Riau University. This research aims to obtain information about hematological profiles of African catfish that are maintained by biofloc systems. The research method used in this study is the survey method. From the sampling location, ware collected as much as 30 African catfish (15 male and 15 female), with a length of 20-25 cm.Sampling was done three times with a span of 14 days. Every sampling ware collected as much as 10 African catfish (5 male and 5 female). Blood sampling is done directly at location and hematological parameter measured at Laboratory. Total erythrocyte and leukocyte measured were Klonz (1994), hemoglobin levels (Wademeyer and Yasutake, 1977), haematocrit and leucocrit levels (Anderson and Siwicki, 1995). The results shows that the hematological value of male African catfishs, total erythrocytes 197.7 x104 cells/mm3, hematocrit 24.4%, hemoglobin 7.3 g/dL, total leukocyte 236.6 x103 cells/mm3, leukocrit 2.86%, while the total erythrocyte of female African catfish is 195.6 x104 cells/mm3, hematocrit 23.7%, hemoglobin 6.7 g/dL, leukocyte total 222.2 x103 cells/mm3, leukocrit 2.26%. Water quality parameters of bioflok ponds during as research were in the ranges, temperature 25-260C, pH 6.7-71, dissolved oxygen 2.8-3.1 mg/L, dan ammonia 0.958-1.235 mg/L.Key Words : Hematological, Clarias gariepinus, Biofloc                                           
SENSITIVITY SOLUTION OF KERSEN LEAVES (Muntingia calabura L.) AGAINST BACTERIAAeromonas Wijaya, Rahmat Santosa; Lukistyowati, Iesje; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Disease that often develops in aquaculture is one of them is red spot disease.This disease is often called Motile Aeromonas Septicemia (MAS) caused byAeromonas hydrophila.Natural ingredients that can be used as prevention and treatment of fish diseases are kersen leaf plant (Muntingia calabura L) which is antibacterial and antimicrobial.This research was conducted in December 2018-February 2019 in the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. The purpose of study was to determine the sensitivity of solution of kersen leaves to inhibit the growth of bacteria Aeromonas hydrophila and to know LD50solution of kersen leaves to jambal siam (Pangasius hypophthalmus) by immersion. Research method used is the experimental method, for sensitivity testusing the method of Kirby Bauerdisc with dosage used were 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 9%, 8%, 7%, 6%, 5% , 4% and controls (Oxytetracyclin). The results showed that the solution of kersen leaves can inhibit the growth of bacteriaA. hydrophila starting from a dose of 100% -4% with an average diameter of inhibition zones ranging from 13.55 - 6.25 mm. LD50 test of the solution of kersen leaf (M. calabura L.) againstjambal siam (P. hypophthalmus) by immersion for 24hoursis at a dose of 3.6%. Keywords: M. calabura L., Motil Aeromonas Septicemia, Kirby Bauer discs, LD50
SENSITIVITY OF THE JUICE OF KAFFIR LIME FRUIT (Citrus hystrix) AGAINST BACTERIA Aeromonas hydrophila Sihombing, Jefry Maju Arytha; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT               Disease that often develops in aquaculture is one of them is red spot disease.This disease is often calledMotile Aeromonas Septicemia (MAS) caused by Aeromonas hydrophila. Alternative ingredients that can be used as prevention or treatment of fish diseases arekaffir lime plant (Citrus hystrix) which is antibacterial and antimicrobial.This research was conducted in February - June 2018 in the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. The purpose of this study was to determine the sensitivity of the juice of kaffir lime to inhibit the growth ofAeromonas hydrophila bacteria, dosage of Minimum Inhibitory Concentration (MIC), and continued the LD50test obtained from the MIC dose where the test of LD50solution of kaffir lime on catfish (Pangasius sp.) by immersion. The research method used was the experimental method, for the sensitivity test using the Kirby Bauer disc method with the concentrations used were 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% , 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6% and controls ( Oxytetracycline). The results showed that the juicce of kaffir lime was able to inhibit the growth of A. hydrophila bacteria starting from doses of 100% - 0.6% with an average diameter of inhibition zones ranging from 23.03 - 6.68 mm. The dose of Minimum Inhibitory Concentration (MIC) juice of kaffir lime fruit is 0.7% with an average number of colonies of 289.33 CFU / mL. The test results of LD50 juice of kaffir lime (C. hystrix) on catfish (Pangasius sp.) By immersion method for 24 hours were at a concentration of 0.791%. Keywords:C.hystrix, Motil Aeromonas Septicemia, Kirby Bauer discs, Minimum                    Inhibitory Concentration (MIC), LD50
THE CURATIVE METHODS OF MAS (Motile Aeromonas Septicaemia) USE LEAF MANGROVE EXTRACT (Rhizophora sp) ON JAMBAL SIAM FISH (Pangasius hypophthalmus) Mastuti, Rini; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThe research was conducted in May until August 2017 in Parasites and Fish Disease Laboratory of Marine and Fisheries Faculty University of Riau,and also in the Integrated Chemical Laboratory of Mathematics and Natural Science of Health Faculty Riau Muhammadiyah University. The purpose of the study was todetermine the ability of Rhizophoraspto cure the disease which is caused by MAS (Motile Aeromonas Septicaemia) and showed from the profile of Siamese Shark (Pangasius hypophthalmus)erythrocyte. The method was an experimental method with a Completely Randomized Design (CRD), one factor with four treatments, there were : control without curing, 1300 ppm, 1600 ppm, and 1900 ppmrespectively. The immersions was applied twice in two days by leaf mangrove extract (Rhizophora s)p for about 5 minutes. The result showed that leaf  mangrove extract (Rhizophora sp) able to cure Siamese Shark from MAS (Motile Aeromonas Septicaemia) by the immersions method. The best dose was at 1600 ppm wich was shown by the total erythrocyte was 290 x 104 cell/mm3, haemoglobin level was 9,30 g/dL, haematocrit value was 29,66 %, and the survival rate was 84,61%.Keywords: Rhizophora sp, Motile Aeromonas Septicaemia, Haematology
KELANGSUNGAN HIDUP IKAN MAS (Cyprinus carpio L) YANG DIBERI PAKAN EKSTRAK BAWANG PUTIH (Allium sativum) DAN DI INFEKSI Aeromonas hydrophila Lukistyowati, Iesje; Kurniasih, Kurniasih
Jurnal Perikanan dan Kelautan Vol 16, No 02 (2011)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.16.02.%p

Abstract

The aim of a research were to investigate the effectiveness of garlic extractmixed with feed given 30 days in encharging common carps after challen with againstAeromonas hydrophila with density of 106 cell/ml. This research conducted by usingcomplete random design with dose addition of garlic extract to feed were controlpositive, control negative, P1 (2,5%), P2 (5%), and P3 (10%). In order to fine out theactive component of garlic extraction methode was adopted using 70% ethanol. Theextracted filtrates were separated using the spectrophotometrical chromatrographymethod (GC-MS) and thin-layered chromatography. Identification of the maincomponents found in the ethanol extract of garlic revealed 13 components but the onethat served as antimicrobial was C6H10S2 CAS disulphide di 2-propenyl(CAS)/(Diallyldisulfide) with and area of 16,95%. Beside the result of thin layeredchromatography showed that garlic extact contained sapononin which is harmful toaquatic animals. Percentage of survival rate P3 (10%) after infected after withAeromonas hydrophila was different compare witn control positive. The highsurvival shown on P3 (10%) concentration with 70%, P1 60%, control negative 60%and P2 30%. Fourteen days after injection the cut cause by Aeromonas hydrophilaclose and the fish recuperated as shown by their lively movement.
Sensitivity of Propolis Solutions on Aeromonas hydrophila Bacteria Bako, Surandha; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Perikanan dan Kelautan Vol 24, No 2 (2019)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.24.2.91-100

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This research was conducted in November 2016 - May 2017 in Parasites and Fish Disease Laboratory of Marine and Fisheries Faculty University of Riau. The purpose of this research is to know the dosage and the ability of propolis solution in inhibiting the growth of Aeromonas hydrophila bacteria and the safe dosage used for common catfish with LD50tested by immersion. The used methodwere experiment and sensitivity test using Kirby Baeur Disk Diffussion method from dose 100% to 0.5% and repeated 3 times. The used dosage in Minimum  Inhibitory Concentration (MIC) tests were 9%, 8%, 7%, 6% and 5%. While LD50 toxicity test using common catfish (Clarias sp) with dose 5%, 6% and 7% for 24 hours. The results showed that the propolis solution was able to inhibit the growth of A. hydrophila bacteria with the smallest dosage of 0.9% equal to 90 ppm, with the diameter inhibitory average zone of 6.78 mm, the safest dose of MIC was present at 6% doses equivalent to 600 ppm with 8,15 mm inhibition zone width  and inhibited bacterial growth  A. hydrophila with an average number of colonies as much as 278 Cells/mL. LD50toxicity test using MIC dosage results showed that no fish mortality for 24 hours.
Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Tamba, Jaya Maruli; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol 26, No 1 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.40-46

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Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Sensitivity of Harumanis Mango Seed (Mangifera indica L) Solution toward Aeromonas hydrophila Telaumbanua, Siti; Lukistyowati, Iesje; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol 24, No 1 (2019)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.24.1.24-31

Abstract

Research was conducted from October to December 2017, at the labory Examination of Fish Disease, Fisheries and Marine Faculty of Universitas Riau. The Purpose of this study was to determine the sensitivity of harumanis mango seed (Mangifera indica L) as antimicrobial to A. hydrophila, the range of Minimal Inhibitory Concentration (MIC) and the lethal dose (LD50) harumanis manggo seed (M. indica L) solution against to catfish (Pangasius pangasius) (7-9 cm) by immersion. The research method used doses; D1: 100%; D2: 90%; D3: 80%; D4: 70%; D5: 60%; D6: 50%; D7: 40%; D8: 30%; D9: 20% D10: 10%, and K : Control (Oxytetracyclin).The results showed that mango seed solution was sensitive to A. hydrophila bacteri and was able to inhibit A. hydrophila up to 0.09% dose with inhibit zone of 7.31 mm. Minimum Inhibitory Concetrantion (MIC) is 0.1% (1000 ppm) number of colonies 270.66 CFU/ mL.The dose of LD50 is 2115,85 ppm