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All Journal Jurnal Akuakultur Rawa Indonesia Jurnal Natur Indonesia Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Jurnal Ilmu Perairan (Aquatic Science) Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Jurnal Perikanan Universitas Gadjah Mada Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Unri Conference Series: Community Engagement Journal of Rural and Urban Community Empowerment Journal of Community Engagement Research for Sustainability Jurnal Abdimas: Pengabdian dan Pengembangan Masyarakat Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan South East Asian Aquaculture Jurnal Akuakultur SEBATIN Jurnal Perikanan dan Kelautan
Iesje Lukistyowati
Fakultas Perikanan Dan Ilmu Kelautan Universitas Riau
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Erythrocyte Profile of Pangasius Hypophthalmus Feed With Rhizophora Apiculata Leaf Extract and Maintained In Net Cages Zissalwa, Fricilia; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol 25, No 1 (2020)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.70-78

Abstract

 Ekstrak daun R. apiculata memiliki manfaat sebagai suplemen herbal yang dapat ditambahkan ke dalam pakan, karena mengandung senyawa flavonoid, tanin, saponin, terpenoid, dan steroid yang dapat meningkatkan kesehatan ikan jambal siam (P. hypophthalmus). Penelitian ini telah dilaksanakan pada bulan Agustus sampai Oktober 2019. Tujuan penelitian adalah untuk mendapatkan dosis terbaik ekstrak daun R. apiculata yang ditambahkan ke dalam pakan untuk meningkatkan kesehatan ikan jambal siam yang dilihat dari total eritrosit, kadar hemoglobin, kadar hematokrit, dan morfologi eritrosit. Metode yang digunakan adalah metode eksperimen dengan Rancangan Acak Lengkap (RAL)satu faktor empat taraf perlakuan terdiri dari P0;(tanpa penambahan ekstrak). P1;(1,5 mg/kg). P2;(1,7 mg/kg). dan P3;(1,9 mg/kg) dan dilakukan ulangan sebanyak tiga kali sehingga diperlukan 12 unit percobaan. Pemeliharaan dilakukan selama 60 hari di dalam keramba dengan ukuran 1x1,5x1 m3. Pemeriksaan darah dilakukan sebanyak tiga kali,yaitu  pada awal pemeliharaan, hari ke-30, dan hari ke-60. Dosis terbaik terdapat pada perlakuan P2 (1,7 mg/kg) dilihat dari total eritrosit 2,57x106 sel/mm3, kadar hemoglobin 13,13 g/dL, nilai hematokrit 38,43%, pertumbuhan bobot mutlak 38,93 g, kelulushidupan 97,33%, dan menunjukkan morfologi eritrosit yang normal didukung oleh kualitas air selama penelitian seperti DO 6,4-6,9mg/L, pH 6,1-6,8, Suhu 28,2-29,5 0C, dan Amoniak 0,04-0,08 mg/L.
Profile of Leukocytes Differentiation of Silver Pompano (Trachinotus blochii) That Were Fed Using Chicken Feather Meal in Fish Diet Fermented by Bacillus sp. Ipa, Adha; Lukistyowati, Iesje; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol 24, No 2 (2019)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.24.2.106-113

Abstract

This research was conducted from July-August 2017. The purpose of the research is to know the effect of adding the chicken feather meal in fish food which was fermented by Bacillus sp. The fermented chicken feather meal was added to the food for adjusting the leukocytes differentiation of Silver Pompano (Trachinotus blochii). The method was an experimental method with a Completely Randomized Design (CRD), one factor with six treatments level. The for 180 fishes. This research used six treatments such as, Kn (negative control): without fermented chicken feather meal by Bacillus sp. in the feed and were not infected by Vibrio alginolyticus, Kp (positive control): without fermented chicken feather meal byBacillus sp. in the feed and were infected by V. alginolyticus, P1-P4 respectively: fermented chicken feather meal by Bacillus sp. in the feed 5%, 10%, 15% and 20%. The collected data is; leukocytestotal, leukocytes differentiation, the average of weight, and survival rate. The results showed that there were a significant effect of adding the fermented chicken feather meal by Bacillus sp.  in the feed to the leukocytes total and leukocytes differentiation of Silver Pompano (Trachinotus blochii). The best treatment was 10% of Bacillus sp.(P2) wich was shown by the80,53x103 cells of leukocytes total, 80,33% of lymphocytes, 9,67 % of monocytes, 10.00 % of Neutrophils, , 5,30 g was the average of weight and100 % of survival rate.
Description of Leukocytes Pangasius hypophthalmus which is Fed with Extracts of Mangrove Leaf (Rhizophora apiculata) and Maintained in The Cages Rahmadona, Zikra; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol 25, No 1 (2020)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.79-87

Abstract

Daun bakau (Rhizophora apiculata) mengandung senyawa flavonoid, steroid, saponin, dan tannin yang berfungsi sebagai antimikrobial dan dapat meningkatkan kesehatan ikan. Penelitian ini telah dilaksanakan pada bulan Agustus sampai dengan bulan Oktober 2019. Tujuan penelitian mendapatkan dosis terbaik ekstrak daun R. apiculata yang ditambahkan ke dalam pakan untuk meningkatkan kesehatan ikan yang dilihat dari total leukosit, diferensiasi leukosit, dan aktifitas fagositosis ikan jambal siam (Pangasius hypophthalmus).Metode yang digunakan adalah metode eksperimen dengan menerapkan Rancangan Acak Lengkap (RAL) satu faktor dengan empat taraf perlakuandan tiga kali ulangan, sehingga diperlukan 12 unit percobaan.Perlakuan tersebut adalah P0 (pakan tanpa mengandung ekstrak daun bakau (R. apiculata), P1, P2, dan P3 pakan yang diberi kandungan ekstrak daun bakau (R. apiculata) dengan dosis 1,5 mg/kg, 1,7 mg/kg, dan 1,9 mg/kg pakan. Ikan yang digunakan adalah ikan jambal siam (P. hypophthalmus) ukuran 8-10 cm dengan padat tebar 50 ekor/m3. Hasil penelitian menunjukkan bahwa dengan penambahan ekstrak daun R. apiculata ke dalam pakan memberikan pengaruh nyata terhadap total leukosit, diferensiasi leukosit, aktifitas fagositosis, kelulushidupan ikan dan pertumbuhan bobot mutlak ikan jambal siam (P. hypophthalmus). Dosis terbaik adalah 1,7 mg/kg pakan dengan total leukosit 11,17x104 sel/mm3, persentase limfosit 84,33%, neutrofil 6,67%, monosit 9,00%, aktifitas fagositosis 35,00%, kelulushidupan 94,67%, dan pertumbuhan bobot mutlak 38,93g. Kualitas air waduk selama penelitian,  suhu antara 28,2-29,50C, DO 6,4-6,9 mg/L, pH 6,1-6,8 dan NH3 0,04-0,08mg/L.
Pengaruh Pemberian Temulawak (Curcuma xanthorriza Roxb) Terhadap Kelulushidupan Ikan Mas (Cyprinus carpio L) Setelah Di Infeksi Aeromonas hydrophila Sari, Novita Winda; Lukistyowati, Iesje; Aryani, Nety
Jurnal Perikanan dan Kelautan Vol 17, No 2 (2012)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.17.2.%p

Abstract

The Effect Of Curcuma (Curcuma xanthorrhiza Roxb) On Survival RateOf Carp Fish (Cyprinus carpio L) After Infected Aeromonas hydrophila. Thestudy was conducted from February to March 2012 in the Laboratory of ParasiticDiseases of Fish and Fisheries and Marine Sciences Faculty of the RiauUniversity. The purpose of this study was to determine the effect of giving asolution of curcuma (Curcuma xanthorriza Roxb) on survival rate of carp(Cyprinus carpio L) after the infected A. hydrophila. This study uses fivetreatments, 1) Kp: positive control (without solution of curcuma and the infectionof A. hydrophila), 2) Kn: negative control (without a solution of curcuma andwithout the infection with A. hydrophila), 3) P1: Curcuma solution with aconcentration of 0.2 g / l, 4) P2: Curcuma solution with a concentration 0.4 g/l, 5)P3: Curcuma solution with a concentration of 0.6 g / l. Providing a solution ofcurcuma with a concentration 0.6 g / l gives the best effect on the survival rate of100%, growth in the absolute weight of 12.48 g / fish and daily growth rate of2.39%.
Leukocytes Differentiation of Goldfish (Carassius auratus) Infected with Aeromonas hydrophila Bacteria and Post-treatment with Propolis Solution Riswan, M.; Lukistyowati, Iesje; Syawal, Henni
Jurnal Natur Indonesia Vol 19, No 1 (2021): April 2021
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.19.1.6-12

Abstract

This study aims to determine the best dose of propolis to treat Carassius auratus infected with Aeromonas hydrophila seen leucocyte differentiation from C.auratus. The method used was experimental method with Completely Randomized Design (CDR) with one factor consisting of five treatment levels and three replications. The treatment was Kn (not infected with A. hydrophila and untreated propolis), Kp (infected with A. hydrophila but not treated with propolis), and fish infected with A. hydrophila treated propolis with dose P1 (700 ppm), P2 (800 ppm), and P3 (900 ppm). Treatment is done by injection of propolis in fish infected by A. hydrophila in intramuscular. The fish used are 8-10 cm in size and kept for 14 days post-infection. The results showed that propolis solution has been able to treat C. auratus from infected A. hydrophila. The dose of propolis 800 ppm is the best dose to treat C. auratus infected A.hydrophila, seen from leukocyte differentiation (lymphocyte 80,25%, monocytes 10,09% and neutrophils 9,66%).
Prevention of Motile Aeromonas Septiceamia in Striped Catfish (Pangasianodon hypophthalmus) using Cherry Leaf Solution Sari, Riski Mutia; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Perikanan dan Kelautan Vol 27, No 1 (2022): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.1.24-31

Abstract

This research was conducted in December 2019 – February 2020 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. The purpose of this study was to analyze and obtain the best dose of cherry leaf (Muntingia calabura) solution to prevent Motile Aeromonas Septicemia from total leukocytes, leukocyte differentiation, and phagocytic activity of striped catfish (Pangasianodon hypophthalmus). The method used is an experiment with a completely randomized design (CRD) one factor five levels of treatment. The doses used were negative control (without immersion cherry leaf solution and not infection with Aeromonas hydrophila), positive control (without immersion cherry leaf solution and infection with A.hydrophila), P1 (Immersion in cherry leaf solution at a dose of 2800 ppm), P2 (3,200 ppm), and P3 (3,600 ppm). Immersion was carried out 5 times for 5 minutes with an interval of 7 days. The results showed that immersion with cherry leaf solution at a dose of 3,600 ppm (P3) was the best dose (P<0.05) with a total leukocyte value of 10.66 x 104 cells/mm3, lymphocytes 84.67%, monocytes 5.67%, neutrophils 9.67%, phagocytic activity 36.67%, and survival rate 86.67%
POTENSI PAKAN YANG MENGANDUNG SAMBILOTO (Andrographis paniculata) DAN DAUN JAMBU BIJI (Psidium guajava) UNTUK MENANGGULANGI BAKTERI Aeromonas hydrophila PADA IKAN BAUNG (Mystus nemurus) Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur Rawa Indonesia Vol 1, No 2 (2013): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.854 KB) | DOI: 10.36706/jari.v1i2.1733

Abstract

An effort to prevent  Aeromonas hydrophila diseases  in catfish (Mystus nemurus)  performed using antibiotic was less safety. Prevention using natural substances mix with fishmeal could overcome the problem. The study was conducted to investigate fismeal potential containing herb of Sambiloto (Andrographis paniculata) and leaves of Jambu biji (Psidium guajava) with different dose given to fish aslong as 60 days. Treatment P1 (without sambiloto flour and jambu biji leaves), P1( 10 g of sambiloto flour + 10 g of jambu biji flour/kg of fish meal), P2 (20 g of sambiloto flour + 20 g of jambu biji flour/ kg of fish meal), P3 (30 g of sambiloto flour + 30 g of jambu biji flour/ kg of fish meal). After 60 days, infection of  Aeromonas hydrophila through intraperitonial with concentration of 108 cell/ml and dose of 0,1ml/fish was conducted. Result of study show that catfish feed with fismeal containing sambiloto  and jambubiji could increase survival rates and growth rates (P˂0,05), but fishmeal containing sambiloto and jambu biji leaves was not effective to privent diseases coused by Aeromonas hydrophila on catfish (Mystus nemurus).   Keywords : Andrographis paniculata, Psidium guajava, Aeromonas hydrophila, catfish (Mystus nemurus) 
Profil Eritrosit Ikan Mas (Cyprinus carpio L) yang Diberi Pakan Mengandung Vaksin Aeromonas hydrophila Isna Sudirman; Henni Syawal; Iesje Lukistyowati
Ilmu Perairan (Aquatic Science) Vol 9, No 2 (2021): Juli 2021
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.9.2.p.144-151

Abstract

Ikan mas (Cyprinus carpio) merupakan jenis ikan air tawar yang banyak dikonsumsi. Salah satu penyakit yang biasa menyerang ikan mas adalah Motile Aeromonos Septiceamia yang disebabkan oleh Aeromonas hydrophila. Vaksinasi adalah pemberian vaksin yang tujuan utnuk meningkatkan kekebalan ikan terhadap bakteri A.hydrophila. Penelitian ini bertujuan untuk menganalisis profil darah merah ikan mas yang diberi vaksin A. hydrophila pada pakan dengan dosis yang berbeda, dan mendapatkan dosis vaksin A. hydrophila terbaik ditambahkan ke pakan. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan menerapkan Rancangan Acak Lengkap (RAL) satu faktor dengan empat taraf perlakuan, yaitu adalah Kn (Pemberian pakan tanpa diberi Vaksin A.hydrophila), P1 (Pemberian Vaksin A.hydrophila dengan Dosis 3 mL/Kg pakan), P2 (4 mL/Kg pakan), dan P3 (5 mL/Kg pakan). Ikan dipelihara dalam keramba berukuran 1x1x1 m sebanyak 12 unit. Ikan yang digunakan berukuran 8-10 cm, dipelihara selama 30 hari. Hasil penelitian menunjukkan bahwa ada pengaruh pemberian A.hydrophila pada pakan terhadap profil darah merah ikan mas yang dipelihara di keramba (P<0,05). Dosis terbaik pemberian vaksin A.hydrophila pada pakan yaitu 5 mL/kg pakan (P3), dilihat dari gambaran darah merah seperti rata-rata total eritrosit 2,12×106sel/mm3, hemoglobin 8,67 g/dL, kadar hematokrit 34,33%, tingkat perlindungan relatif 66,67%, dan kelulushidupan 97,33 %.
Penambahan Larutan Daun Kersen (Muntingia calabura L.) dalam Pakan untuk Mencegah Penyakit Edwarsiliosis pada Ikan Jambal Siam (Pangasianodon hypophthalmus Dharga Mufti. Jr; Iesje Lukistyowati; Morina Riauwaty
Ilmu Perairan (Aquatic Science) Vol 10, No 1 (2022): Maret 2022
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.10.1.p.21-30

Abstract

Penelitian ini dilaksanakan pada bulan Desember 2019 s/d Februari 2020 bertempat di Laboratorium Parasit dan Penyakit Ikan Fakultas Perikanan dan Kelautan Universitas Riau. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan larutan daun kersen (Muntingia calabura L.) terhadap gambaran leukosit dan mendapatkan dosis terbaik penambahan larutan daun kersen ke dalam pakan. Metode yang digunakan adalah metode eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL) dengan lima taraf perlakuan yaitu Kn= Kontrol negatif (pemberian pakan tanpa penambahan kersen), Kp= Kontrol positif (pemberian pakan tanpa penambahan kersen), P1= penambahan larutan daun kersen dengan dosis 1 mL/kg Pakan, P2= penambahan larutan daun kersen dengan dosis 2 mL/kg pakan dan P3= penambahan larutan daun kersen dengan dosis 3 mL/kg pakan. Hasil penelitian menunjukkan larutan daun kersen berpengaruh nyata terhadap gambaran leukosit ikan jambal siam (Pangasianodon. hypophthalmus) dilihat dari adanya peningkatan total leukosit, diferensiasi leukosit, dan aktivitas fagositosis (P<0,05). Dosis terbaik terdapat pada perlakuan P3 (3 mL/kg pakan) dengan rata-rata jumlah leukosit 9,38x104 sel/mm3, aktivitas fagositik sebesar 31,67%, dan tingkat kelulushidupan mencapai 86,67%. Sedangkan persentase limfosit 83,00%, monosit 9,00% dan neutrofil 8,00%.
TOTAL DESCRIPTION OF ERITROSIT, HEMOGLOBIN CONTENT AND THE VALUE OF FISH HEMATOKRITES Oreochromis niloticus) WITH FOOD CONTAINED Bacillus sp Dinar Mey Jasika H; Iesje Lukistyowati; Henni Syawal
Berkala Perikanan Terubuk Vol 45, No 3 (2017): November 2017
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.808 KB) | DOI: 10.31258/terubuk.45.3.23-43

Abstract

This research was conducted in October 2015-June 2016 which was held at Marine Microbiology Laboratory, Parasitic and Fish Disease laboratory and Aquaculture Technology Laboratory Faculty of Fisheries and Marine University of Riau, Pekanbaru. This study aims to determine the effectiveness of giving Bacillus sp. Which is isolated from giant prawns (Macrobrachium rosenbergii de Man) and tiger prawns (Penaeus monodon) into the diet can improve the health oftilapia (Oreochromis niloticus) seen the total picture of erythrocytes, hemoglobin and hematocrit values. The method used by using Completely Randomized Design (RAL), ie in each treatment done probiotik Bacillus sp. From prawns and tiger prawns using 3 levels of treatment and 3 replications. This research uses application method by spraying probiotic Bacillus sp. On commercial feed. As probiotic treatments were used Bacillus sp. The best isolate of UG4 isolated from digestive tract of giant prawns. As for the isolates of the digestive tract of tiger shrimp used probiotics Bacillus sp. The best isolate is UWH9. The result of this research shows that P2 (UWH9) treatment is the best isolate from the digestive tract of tiger shrimp with total erosite value of 205 x 104 cell / mm3, hemoglobin level 5.05 g / dL, hematocrit value 30%, absolute weight growth Of 23.16 g, and a survival rate of 80%. 
Co-Authors Adelina Adelina Agustina Bertha, Agustina Ahmad, Nurkholis Alfinda, Rudi Alim Isnansetyo Andre A Saragih, Andre A Aryani, Nety Bako, Surandha Batara, Lamun Batubara, Ummi Mardhiah Dessy Yoswati Dharga Mufti. Jr Dinar Mey Jasika H Feliatra Feliatra, Feli Ghazali, Tengku Muhammad Henni Syawal Henny Syawal Ilka, Ade Hidayat Indra Suharman Ipa, Adha Irawan, Chairani Irma Yunita Isna Sudirman Jr, Dharga Kahfi, Khaiqal El Kurniasih Kurniasih Kurniasih Kurniasih Kurniawan, Ahmad Juli Lamun Batara Lestari, Rona Malau, Diana P Mardalisa, Mardalisa Mario Syatma Mario Syatma, Mario Morina Morina Muhammad Muhajir, Muhammad Muhammad Ridwan Mulyadi . Nanang Muhson Nasution, Ahmad Tirmizi Netti Aryani Niken Ayu Pamukas Niken Ayu Pamungkas Nurfitri, Maya Pipin Kurnia Purba, Yoshua Dewaman Mangapul Purwanto Purwanto Putri, Atika Putri, Mega Novia Putri, Rianti Rahmadona, Zikra Rini Mastuti Riswan, M Ronal Kurniawan Santi Ramayani, Santi Sari, Novita Winda Sari, Nur Maya Sari, Riski Mutia Sihombing, Jefry Maju Arytha Simanjuntak, Lewi Maraditha Sinaga, Nurilam Sudirman, Isna Syaieba, Mutiara Tamba, Jaya Maruli Telaumbanua, Siti Ulandari, Ulfa Usman Muhammad Tang Wijaya, Rahmat Santosa Windarti Windarti Yunita Paramitha Hasibuan Zebua, Ratna Dewi Zissalwa, Fricilia