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Isolation of Lactic Acid Bacteria from Digestive Tract of Asian Swamp Eel (Monopterus albus) and Bacterial Antagonist Test to Inhibit Pathogenic Bacteria Aeromonas hydrophila and Pseudomonas sp Alfinda, Rudi; Lukistyowati, Iesje; Syawal, Henni; Putra, Iskandar
Jurnal Perikanan dan Kelautan Vol. 27 No. 3 (2022): October
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Lactic Acid Bacteria (LAB) are probiotic-forming bacteria that have characteristics, like be able to ferment sugar or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this study was to obtain isolates of lactic acid bacteria from the digestive tract of Asian Swamp Eel (Monopterus albus) and to determine the inhibition produced by these lactic acid bacteria in inhibiting pathogenic bacteria such as Aeromonas hydrophila and Pseudomonas sp. The method was used in this study is experimental method, by taking the digestive tract of Asian Swamp Eel and then identifying the lactic acid bacteria and characterized by morphological and biochemical characteristics and tested of the antagonist against the pathogenic bacteria A.hydrophila and Pseudomonas sp. using the Kirby Bauer disc method (disc method). From the research results obtained 5 types of candidate lactic acid bacteria with each code BL121, BL263, BL142, BL242 and BL342. The five isolates of lactic acid bacteria were able to inhibit A.hydrophila and Pseudomonas sp. with a Inhibition range of 8-16 mm. Based on the results of the study, there were three isolates that had inhibitory power against bacteria A. hydrophila (12-16 mm) and Pseudomonas sp (11-13 mm) with codes BL121, BL263 and BL142 and were classified as strong. The identification results obtained are bacteria from the genus Bacillus sp.
Gejala Klinis Ikan Komet (Carassius auratus) yang Terinfeksi Bakteri Aeromonas hydrophila dan Pascapengobatan dengan Larutan Propolis M. Riswan; Lukistyowati, Iesje; Syawal, Henni; Riauwaty, Morina; Alfinda, Rudi; Kurniawan, Ronal; Putri, Mega Novia
South East Asian Aquaculture Vol. 3 No. 1 (2025): Juli
Publisher : Science, Technology, and Education Care

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61761/seaqu.3.1.1-8

Abstract

Penelitian ini bertujuan untuk mengetahui gejala klinis ikan komet yang terinfeksi bakteri Aeromonas hydrophila dan pascapengobatan dengan larutan propolis. Metode yang digunakan adalah metode eksperimen dengan menerapkan rancangan acak lengkap (RAL) dengan satu faktor lima taraf perlakuan dan tiga kali ulangan perlakuan. Perlakuan tersebut adalah adalah Kn (tidak terinfeksi A.hydrophila dan tidak diobati propolis), Kp (terinfeksi A. hydrophila  tetapi tidak diobati propolis), sedangkan ikan terinfeksi A.hydrophila diobati dengan propolis dosis P1 (700 ppm), P2 (800 ppm), dan P3 (900 ppm). Pengobatan dilakukan dengan cara penyuntikan propolis pada ikan yang terinfeksi A.hydrophila di bagian intramuscular sebanyak 0,1 ml. Bahan uji yang digunakan adalah ikan komet (Carassius auratus) ukuran 8-10 cm sebanyak 150 ekor. Hasil penelitian menunjukkan bahwa ikan yang terinfeksi bakteri A.hydrophila menunjukkan gejala klinis sirip ekor gripis, bercak merah, mata menonjol (exopthalmia), perut mengembung (dropsy), sisik tubuh lepas. Setelah 14 hari pascapengobatan dengan larutan propolis perlakuan terbaik adalah perlakuan P2 dosis (800 ppm) mengalami kondisi pemulihan mendekati gejala klinis kondisi ikan normal terlihat dari warna tubuh cerah, produksi lendir normal, sirip ekor utuh, tidak terdapat mata menonjol (exopthalmia), dan perut tidak mengembung (dropsy).
RESPONSE IMMUNITY OF GOLDFISH (Carassius auratus) INFECTED WITH Aeromonas hydrophila BACTERIA AND POST-TREATMENT WITH PROPOLIS SOLUTION Riswan, M; Lukistyowati, Iesje; Syawal, Henni; Kurniawan, Ronal; Putri, Rianti; Putri, Mega Novia
Asian Journal of Aquatic Sciences Vol. 8 No. 2 (2025): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.2.223-229

Abstract

This study aims to determine the immunity response of  Carassius auratus infected with Aeromonas hydrophila bacteria and post-treatment with propolis solution by measuring total leukocytes and phagocytosis index. The method used is experimental by applying a completely randomized design (CRD) with five treatments and three replications. The treatments were Kn (not infected with A. hydrophila and not treated with propolis), Kp (infected with A. hydrophila but not treated with propolis), while fish infected with A. hydrophila were treated with propolis doses P1 (700 ppm), P2 (800 ppm), and P3 (900 ppm). Treatment was done by injecting 0.1 ml of propolis into fish infected with A. hydrophila intramuscularly. The test material was 150 fish of 8-10 cm in size. The results showed that propolis significantly gave an immune response to the C. auratus infected with A. hydrophila (p<0.05). The 800 ppm propolis dose was the most effective, as indicated by a total leukocyte count of 3.70×104 cells/mm3, a phagocytosis index value of 28.33%, and a survival rate of 83.33%. This study highlights that propolis has potential as a natural immunostimulatory agent in enhancing non-specific defense mechanisms in C. auratus infected with pathogenic bacteria
Peningkatan Pendapatan Nelayan Di Desa Teluk Rhu Kabupaten Bengkalis Melalui Teknologi Diversifikasi Pangan Berbahan Dasar Hasil Perikanan Feliatra, Feliatra; Batubara, Ummi Mardhiah; Mardalisa, Mardalisa; Kurnia, Pipin; Lukistyowati, Iesje; Ghazali, Tengku Muhammad
Journal of Community Engagement Research for Sustainability Vol. 2 No. 2 (2022): Maret
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.668 KB) | DOI: 10.31258/cers.2.2.70-77

Abstract

Teluk Rhu Village is a village that has potential in terms of fisheries and marine tourism. Some of the people in this village are fishermen, and the rest are fish farmers. The minimum knowledge and competence have fishermen in Teluk Rhu Village cause the welfare to be relatively low. To improve the fishermen's income needed knowledge improvement through food diversification technology based on fishery products. There are four methods used in this program, such as observation, material delivery, product manufacturing practices, and program evaluation. Twenty fishermen were participants and were involved very enthusiastically and actively in this program. There were two materials presented to increase fishermen's knowledge, including digital literacy and food innovation products. Lastly, all participants were trained to make several processed food products such as fish balls, nuggets, and fish sticks. The questionnaire data show that the community service program had several benefits for most participants. Thus, it's hoped that the new knowledge can make them more enthusiastic and motivated to begin a new businesses to increase family income.