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All Journal Jurnal Akuakultur Rawa Indonesia Jurnal Natur Indonesia Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Jurnal Ilmu Perairan (Aquatic Science) Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Jurnal Perikanan Universitas Gadjah Mada Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Unri Conference Series: Community Engagement Journal of Rural and Urban Community Empowerment Journal of Community Engagement Research for Sustainability Jurnal Abdimas: Pengabdian dan Pengembangan Masyarakat Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan South East Asian Aquaculture Jurnal Akuakultur SEBATIN Jurnal Perikanan dan Kelautan
Iesje Lukistyowati
Fakultas Perikanan Dan Ilmu Kelautan Universitas Riau
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Isolation of Lactic Acid Bacteria from Digestive Tract of Asian Swamp Eel (Monopterus albus) and Bacterial Antagonist Test to Inhibit Pathogenic Bacteria Aeromonas hydrophila and Pseudomonas sp Alfinda, Rudi; Lukistyowati, Iesje; Syawal, Henni; Putra, Iskandar
Jurnal Perikanan dan Kelautan Vol. 27 No. 3 (2022): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Lactic Acid Bacteria (LAB) are probiotic-forming bacteria that have characteristics, like be able to ferment sugar or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this study was to obtain isolates of lactic acid bacteria from the digestive tract of Asian Swamp Eel (Monopterus albus) and to determine the inhibition produced by these lactic acid bacteria in inhibiting pathogenic bacteria such as Aeromonas hydrophila and Pseudomonas sp. The method was used in this study is experimental method, by taking the digestive tract of Asian Swamp Eel and then identifying the lactic acid bacteria and characterized by morphological and biochemical characteristics and tested of the antagonist against the pathogenic bacteria A.hydrophila and Pseudomonas sp. using the Kirby Bauer disc method (disc method). From the research results obtained 5 types of candidate lactic acid bacteria with each code BL121, BL263, BL142, BL242 and BL342. The five isolates of lactic acid bacteria were able to inhibit A.hydrophila and Pseudomonas sp. with a Inhibition range of 8-16 mm. Based on the results of the study, there were three isolates that had inhibitory power against bacteria A. hydrophila (12-16 mm) and Pseudomonas sp (11-13 mm) with codes BL121, BL263 and BL142 and were classified as strong. The identification results obtained are bacteria from the genus Bacillus sp.
Red Blood Profile Of Monopterus Albus Preserved In Multitesely Systems With Different Stock Densities Muhajir, Muhammad; Lukistyowati, Iesje; Syawal, Henni
Jurnal Akuakultur SEBATIN Vol. 4 No. 1 (2023): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.1.12-21

Abstract

Biofloc is a technology using good bacteria to convert organic waste to be food source by fish in the form of floc. This research was carried out in April-May 2021 in the Hatchery and blood observations at the Fish Disease and Parasite Laboratory, Faculty of Fisheries and Marine, University of Riau. The purpose of this study was to determine the health status of eels from the profile of erythrocytes cells and the best stocking density of eels cultivated in biofloc system. While the benefits of this study can be applied to biofloc cultivation and apply it in the field. The research method used one-factor completely randomized design (CRD) with four treatment levels and three replications (12 experimental units) for 40 days . The optimal stocking density of eels in clean water is 6 fish for 10 liters. The treatments in this study were P1: stocking density of 10 fish/25 L, P2: stocking density of 20 fish/25 L, P3: stocking density of 30 fish/25 L, P4: stocking density of 40 fish/25 L. The results showed that the erythrocytes profile of eel with different stocking densities had a significant effect (P<0.05) and the best treatment P3 (30 fish/25 L) with total erythrocytes 2.88x106 cells/mm3, hematocrit level 31.66 %, hemoglobin levels 17.40 g/dL, blood glucose 46.33 g/dL, specific growth rate 93.3%, and feed efficiency 67.46%. Water quality in this study was in normal range, that is temperature 25.7-27.3 oC, pH 7.0-7.8, DO 6.4-8.0 mg/L, and NH3 0.0003-0.00038 mg/L.
Identification Of Clinostomum sp. Parasites In Swamp Sepat Fish (Trichogaster trichopterus) In FAPERIKA DAM, Pekanbaru Nasution, Ahmad Tirmizi; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Akuakultur SEBATIN Vol. 4 No. 1 (2023): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.1.49-57

Abstract

Parasites are organisms that live on other organisms that can benefit from the symbiosis, while the host is harmed. This research was conducted from March to Mei 2022 at the Laboratory of Fish Parasites and Diseases, Department of Aquaculture, Faculty of Fisheries and Marine. Trichogaster trichopterus sampling was carried out Feperika Dam, Pekanbaru. The aim of the study was to obtain the prevalence and intensity of the parasite Clinostomum sp. which infects the swamp fish (Trichogaster trichopterus) in Faperika Dam, Pekanbaru. The method used is a survey method and direct observation of the sample. Sampling was carried out three times with sampling points consisting of 3 places, namely: Location 1 is at the entrance of the reservoir, locations 2 and 3 are located to the west and north of location 1 with a distance of each location 50 meters. The results of the identification showed that the sample of bog fish infected with trematode parasites was Clinostomum sp. with prevalence ranging from 93.9-100%, prevalence in locations 1 and 2 (100%), and intensity for each location, namely, at location 1 at 36.9 ind/bird, location 2 at 41.3 ind and location 3 is 34.3 ind.
Administration of Probiotics in Recirculating System Cultivation to Improve the Health of Colossoma macropomum Sinaga, Nurilam; Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.101-110

Abstract

Probiotic containing Nitrosomonas sp and Nitrobacter sp play a role in the nitrification process to improve water quality in the media maintenance. This research was conducted in July – October 2021 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University. The purpose of this study was to obtain the best dose of Kp Super N probiotics for fish health. The method used is an experimental method using a one-factor with five treatment levels and three replications. The treatments applied were Cn (maintenance without probiotic Kp Super N and without being challenged with Aeromonas hydrophila), Cp (maintenance without probiotic Kp Super N and challenged with A. hydrophila), maintenance was given probiotic Kp Super N and challenged with A. hydrophila: P1 (2 mL/80 L of water), P2 (3 mL/80 L), P3 (4 mL/80 L). Fish were reared for 72 days. The results shown that the best treatment was P3 (4 mL/80 L) with a total erythrocyte of 2,70 x 106 cells/mm3, haematocrit value of 36,00%, and hemoglobin level of 8,17 g/dl, total leukocytes 8,17 x104 cells/mm3, leukocrite 3,17%, lymphocytes 63,00%, monocytes 4,00%, neutrophils 1,00%, and platelets 32,00%. Weight growth was 23,29 g and the survival rate 100%. The water quality temperature 26 – 29,7oC, pH 6 – 7,5, DO 4,9 – 5,2 ppm, ammonia 0,05430-0,00062 mg/L, nitrite 0,0549-0,1709 mg/L, and nitrate 0,2000-3,6670 mg/L. Based on the date obtain, it can be concluded that the probiotic Kp Super N is able to improve the health of Colossoma macropomum
Hematology of Osphronemus goramy Fed with Fermented Vegetable Waste with Cows Rumen Ilka, Ade Hidayat; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.111-125

Abstract

Vegetables in chicory and cabbage contain flavonoid compounds, alkaloids, saponins, glycosides, vitamin C, glucosinolates, glutamine, and sulforaphane, which have antimicrobial activity that can be used as immunomodulators that can strengthen the immune system. This research was conducted from February to April 2022 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to analyze the effect of fermenting mustard and cabbage waste flour in artificial feed on goramy hematology. The method used is an experimental method using a one-factor Completely Randomized Design (CRD) with five treatment levels and three replications. The treatments were P0 (Feed without added fermented chicory and cabbage and challenge with Aeromonas hydrophila), and fish were fed with fermented chicory and cabbage with doses of P1 (15%), P2 (25%), P3 (35%), P4 (45%) and challenged with A. hydrophila. Fishes were reared for 60 days with feed containing fermented chicory and cabbage, and the final data was 14 days of testing with A. hydrophila with the same feed. The results showed that the best dose of feed containing fermented chicory and cabbage was in treatment P2 (25%) with a total erythrocyte of 2.21 x106 cells/mm3, a hemoglobin level of 5.40 g/dL, and a hematocrit value of 38.67%. Tested with A. hydrophila bacteria, total erythrocytes 2.68 x106 cells/mm3, hemoglobin 6.60 g/dL, hematocrit value 39.00%, survival rate 96, 67%, absolute weight growth 1.06 g. Data obtained on O. goramy fed with fermented vegetable waste with cows' rumen showed an effect on improving fish hematology.
Leukocytes Profil of Monopterus albus raired in Biofloc System with Different Stock Densities Ahmad, Nurkholis; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Akuakultur SEBATIN Vol. 5 No. 2 (2024): November
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.2.137-145

Abstract

Biofloc is an agglomerate composed of various autotrophic and heterotrophic organisms as well as particles, which are mixed by aeration and circulation to form clumps that integrate quite well in water. This research was carried out in March- May 2021 in the Hatcheries and Experimental Ponds and Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The study aimed to analyze the leukocyte profile of the Monopterus albus raired biofloc system with different stock densities. The research method used a one-factor, completely randomized design (CRD) with four treatments with three replications. The treatments applied were P1 (of 10 fish/25 L), P2 (of 20 fish/25 L), P3 (of 30 fish/25 L), and P4 (of 40 fish/25 L). M. albus were reared in a biofloc system for 40 days. The results showed that there was an effect of stock densities on the leukocyte profile of M. albus reared in biofloc systems with different stock densities, a significant effect (P<0.05). The best stock density was at P3 (30 fish/25 L), where the total leukocytes were 3.62 x 104 cells/mm3, leucocrite was 2.00%, phagocyte activity was 36.66%, while the percentage of lymphocytes was 83.00 %, monocytes was 16.00%, and neutrophils was 13.00%. Water quality during this study was at temperature 26-300C, pH 6-7, dissolved oxygen 4.4-6.8 mg/L, and ammonia 0.02-0.06 mg/L. It was concluded that there was an effect on the biofloc system of stock density on the leucocyte profile M. albus
Growth Performance of Striped Catfish (Pangasianodon hypopthalmus) Fed by Fermented Water Hyacinth Ramayani, Santi; Suharman, Indra; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol. 27 No. 3 (2022): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Fermented water hyacinth by Aspergillus niger can be used as alternative ingredient replacing soymeal for feed in striped catfish. The experimental designed consist of five treatment substitution of soymeal used fermented water hyacinth and three repliacate: T0 (feed without FWH); T1 (25% FWH); P2 ( 50% FWH); P3 (75% FWH); and P4 (100% FWH. ). A total of 375 Striped catfish weight 4.21 ± 0.42 g were stocked with a density of 25 fish m-3 in fixed net cages. The study wasconducted for 42 days. The results showed that the treatment given improved growth, feed convertion, and survival rate of striped catfish at dose FWH 50% It is recommended that substitution FWH 50% can used to replacing soymeal for feed striped catfish.
Isolation of Lactic Acid Bacteria from Digestive Tract of Asian Swamp Eel (Monopterus albus) and Bacterial Antagonist Test to Inhibit Pathogenic Bacteria Aeromonas hydrophila and Pseudomonas sp Alfinda, Rudi; Lukistyowati, Iesje; Syawal, Henni; Putra, Iskandar
Jurnal Perikanan dan Kelautan Vol. 27 No. 3 (2022): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Lactic Acid Bacteria (LAB) are probiotic-forming bacteria that have characteristics, like be able to ferment sugar or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this study was to obtain isolates of lactic acid bacteria from the digestive tract of Asian Swamp Eel (Monopterus albus) and to determine the inhibition produced by these lactic acid bacteria in inhibiting pathogenic bacteria such as Aeromonas hydrophila and Pseudomonas sp. The method was used in this study is experimental method, by taking the digestive tract of Asian Swamp Eel and then identifying the lactic acid bacteria and characterized by morphological and biochemical characteristics and tested of the antagonist against the pathogenic bacteria A.hydrophila and Pseudomonas sp. using the Kirby Bauer disc method (disc method). From the research results obtained 5 types of candidate lactic acid bacteria with each code BL121, BL263, BL142, BL242 and BL342. The five isolates of lactic acid bacteria were able to inhibit A.hydrophila and Pseudomonas sp. with a Inhibition range of 8-16 mm. Based on the results of the study, there were three isolates that had inhibitory power against bacteria A. hydrophila (12-16 mm) and Pseudomonas sp (11-13 mm) with codes BL121, BL263 and BL142 and were classified as strong. The identification results obtained are bacteria from the genus Bacillus sp.
Profil Eritrosit Ikan Mas (Cyprinus carpio L) yang Diberi Pakan Mengandung Vaksin Aeromonas hydrophila Sudirman, Isna; Syawal, Henni; Lukistyowati, Iesje
Jurnal Ilmu Perairan (Aquatic Science) Vol. 9 No. 2 (2021): Juli
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Ikan mas (Cyprinus carpio) merupakan jenis ikan air tawar yang banyak dikonsumsi. Salah satu penyakit yang biasa menyerang ikan mas adalah Motile Aeromonos Septiceamia yang disebabkan oleh Aeromonas hydrophila. Vaksinasi adalah pemberian vaksin yang tujuan utnuk meningkatkan kekebalan ikan terhadap bakteri A.hydrophila. Penelitian ini bertujuan untuk menganalisis profil darah merah ikan mas yang diberi vaksin A. hydrophila pada pakan dengan dosis yang berbeda, dan mendapatkan dosis vaksin A. hydrophila terbaik ditambahkan ke pakan. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan menerapkan Rancangan Acak Lengkap (RAL) satu faktor dengan empat taraf perlakuan, yaitu adalah Kn (Pemberian pakan tanpa diberi Vaksin A.hydrophila), P1 (Pemberian Vaksin A.hydrophila dengan Dosis 3 mL/Kg pakan), P2 (4 mL/Kg pakan), dan P3 (5 mL/Kg pakan). Ikan dipelihara dalam keramba berukuran 1x1x1 m sebanyak 12 unit. Ikan yang digunakan berukuran 8-10 cm, dipelihara selama 30 hari. Hasil penelitian menunjukkan bahwa ada pengaruh pemberian A.hydrophila pada pakan terhadap profil darah merah ikan mas yang dipelihara di keramba (P<0,05). Dosis terbaik pemberian vaksin A.hydrophila pada pakan yaitu 5 mL/kg pakan (P3), dilihat dari gambaran darah merah seperti rata-rata total eritrosit 2,12×106sel/mm3, hemoglobin 8,67 g/dL, kadar hematokrit 34,33%, tingkat perlindungan relatif 66,67%, dan kelulushidupan 97,33 %.
Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Tamba, Jaya Maruli; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Co-Authors Adelina Adelina Agustina Bertha, Agustina Ahmad, Nurkholis Alfinda, Rudi Alim Isnansetyo Andre A Saragih, Andre A Aryani, Nety Bako, Surandha Batara, Lamun Batubara, Ummi Mardhiah Dessy Yoswati Dharga Mufti. Jr Dinar Mey Jasika H Feliatra Feliatra, Feli Ghazali, Tengku Muhammad Henni Syawal Henny Syawal Ilka, Ade Hidayat Indra Suharman Ipa, Adha Irawan, Chairani Irma Yunita Isna Sudirman Jr, Dharga Kahfi, Khaiqal El Kurniasih Kurniasih Kurniasih Kurniasih Kurniawan, Ahmad Juli Lamun Batara Lestari, Rona Malau, Diana P Mardalisa, Mardalisa Mario Syatma Mario Syatma, Mario Morina Morina Muhammad Muhajir, Muhammad Muhammad Ridwan Mulyadi . Nanang Muhson Nasution, Ahmad Tirmizi Netti Aryani Niken Ayu Pamukas Niken Ayu Pamungkas Nurfitri, Maya Pipin Kurnia Purba, Yoshua Dewaman Mangapul Purwanto Purwanto Putri, Atika Putri, Mega Novia Putri, Rianti Rahmadona, Zikra Rini Mastuti Riswan, M Ronal Kurniawan Santi Ramayani, Santi Sari, Novita Winda Sari, Nur Maya Sari, Riski Mutia Sihombing, Jefry Maju Arytha Simanjuntak, Lewi Maraditha Sinaga, Nurilam Sudirman, Isna Syaieba, Mutiara Tamba, Jaya Maruli Telaumbanua, Siti Ulandari, Ulfa Usman Muhammad Tang Wijaya, Rahmat Santosa Windarti Windarti Yunita Paramitha Hasibuan Zebua, Ratna Dewi Zissalwa, Fricilia