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PROFILE ERYTHROCYTES OF Oreochromis niloticus FED WITH HERBS FERMENTED AND INFECTED WITH Aeromonas hydrophila Nurfitri, Maya; Syawal, Henni; Lukistyowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Herbs Fermented consist of Kaempferia galanga L, Curcuma xanthorrhiza Roxb, and Curcuma domestica Val, which are environmentally friendly disease prevention alternatives that can be added to feed because they have several advantages, such as increasing appetite, digestion, growth, disease resistance, and reducing stress levels in fish. This research was carried out from August to December 2021 at the Marine Science Biotechnology Laboratory and Fish Parasites and Diseases Laboratory, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to obtain the best dose from adding fermented herbs to increase the immune system of Oreochromis niloticus and prevent diseases caused by Aeromonas hydrophila bacteria in terms of profile erythrocytes. The method used was an experimental method using a Completely Randomized Design (CRD) with one factor, five levels of treatment, and three replications so that 15 experimental units were obtained. The challenge test was carried out on the 32nd day using A.hydrophila with a density of 108 CFU/mL. The best dose was found in P3 with a dose of 200 mL/kg of feed, which showed a total erythrocyte of 2.09x106 cells/mm3, hemoglobin level of 7.70 g/dL, hematocrit value of 37.70%, and a normal erythrocyte morphology. Water quality ranged from temperature 27-29oC, pH 6.9-7.2, DO 3.1-4.4 mg/L, and Ammonia 0.016-0.024 mg/L. Based on the data obtained, it can be concluded that fermented herbs can increase the immunity of O. niloticus to diseases caused by A.hydrophila.
HEMATOLOGY OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) REARED IN MEDIA WITH REGULAR ADDITION OF PROBIOTICS Lestari, Rona; Syawal, Henni; Lukistyowati, Iesje
Asian Journal of Aquatic Sciences Vol. 6 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Multi-cell probiotic containing bacteria Nitrosomonas sp., Nitrobacter sp., and Bacillus sp. This research was conducted from March to May 2021 at the Fish Disease and Parasite Laboratory, Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to analyze the hematology of Asian redtail catfish reared in media that was added with probiotics periodically and to obtain the best time interval for giving probiotics to the rearing medium. The method used in this study is an experimental method by applying a Completely Randomized Design (CRD) with five treatment levels and three replications. The treatments used were the administration of probiotics at a dose of 0,01 mL/L of water with different time intervals, P0 (without treatment), P1 (5 days once), P2 (10 days once), P3: (15 days once), and P4 (20 days once). Fish were reared for 50 days. The results show that the Asian redtail catfish reared with the addition of probiotics in the rearing medium had a significant effect (P<0.05) with the best time interval being once every 5 days in the P1 treatment, seen from the total erythrocytes 2.43 x 106 cells/mm3, hematocrit level 28.67%, hemoglobin level 7.40 g/dL, total leukocytes 9.92 x 104 cells/mm3, leukocrit level 1.83%, and glucose 49.67 g /dL. Water quality during the study was still in the normal range that could support the growth of Asian redtail Catfish, namely temperature 26-27.1 C, pH 5.8-6.5, DO 6.0-7.4 mg/L, and ammonia 0.0011-6.5 0.0047 mg/L.
INTESTINE AND LIVER HISTOPATHOLOGY OF STRIPED CATFISH (Pangasianodon hypophthalmus) FEEDING CONTAINING PAPAYA LEAF FERMENTATION Purba, Yoshua Dewaman Mangapul; Syawal, Henni; Lukistyowati, Iesje
Asian Journal of Aquatic Sciences Vol. 6 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Aeromonas hydrophila bacteria often attack internal organs such as the liver and intestines. This research was conducted in June – August 2021 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. Histology preparations were made at the Bukittinggi Veterinary Center. This research aimed to obtain the best dose addition of papaya leaf fermented solution which could reduce intestinal and liver tissue damage of striped catfish (P.hypophthalmus) which was challenged with A.hydrophila. The method used is an experimental method with a completely randomized design (CRD) with 4 treatment levels and 3 replications; Kp (Feed not given papaya leaf fermented solution + infected with A.hydrophila), P1 (16 ml/kg feed of papaya leaf fermented solution + infected with A.hydrophila), P2 (16 ml/kg feed of papaya leaf fermented solution) + infected with A.hydrophila), P3 (16 ml/kg of papaya leaf fermented solution feed + infected with A. hydrophila). The fish used was 8-10 cm with a density of 1 fish/3 L totaling 10 fish with a container measuring 40x30x30 cm. Infection by injection in the intramuscular section as much as 0.1 mL/head with a bacterial density of 108 CFU/mL. The parameters observed were clinical symptoms for 72 hours and histopathology of the intestine and liver of striped catfish. The results showed that the intestine saw the presence of hemorrhage, necrosis, and edema. While the liver shows abnormalities, such as hemorrhage, necrosis, hypertrophy, and fatty degeneration.
PEMBERDAYAAN ISTRI NELAYAN DALAM UPAYA PENINGKATAN PENGHASILAN RUMAH TANGGA NELAYAN DI DESA TELUK RHU KABUPATEN BENGKALIS PROPINSI RIAU Feliatra, Feli; Batubara, Ummi Mardhiah; Lukistyowati, Iesje; Mardalisa, Mardalisa; Batara, Lamun
Journal of Rural and Urban Community Empowerment Vol. 4 No. 2 (2023): April
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.483 KB)

Abstract

Desa Teluk Rhu merupakan salah satu desa yang terdapat di kecamatan Rupat Utara, yang memiliki potensi yang tinggi di bidang perikanan dan wisata bahari. Desa Teluk Rhu memiliki jumlah penduduk 1.885 orang yang terdiri dari 364 KK. Salah satu masalah saat ini ini adalah masih rendahnya pengetahuan keluarga nelayan terhadap pengolahan hasil tangkapan nelayan. Tujuan pengabdian adalah untuk meningkatkan pengetahuan dua kelompok istri nelayan Desa Teluk Rhu tentang pengolahan hasil perikanan menjadi produk olahan yaitu nugget, bakso, kemplang dan stik ikan dari ikan hasil tangkapan. Metode yang digunakan pada kegiatan ini adalah dengan metode ceramah, diskusi dan praktek pembuatan produk olehan ikan dan evaluasi. Materi penyuluhan yang disampaikan adalah: 1. Pentingnya packaging dan performance barang jualan, 2. Pembuatan keplak, 3. Pembuatan stik ikan. 4. Pembuatan otak-otak, 5. Pembuatan bakso bagi kelompok istri nelayan. Peserta diikuti dua kelompok keluarga nelayan. Dari hasil pengabdian dapat disimpulkan pelatihan sangat bermanfaat bagi peserta, Dapat meningkatan perolehan hasil tambahan, peserta menganggap bahwa 4 produk yang sudah dikenalkan bisa menjadi produk khas desa teluk Rhu Kecamatan Rupat utara, jika pembinaan terus menerus bisa dilaksanakan.
Pencegahan Penyakit Motile Aeromonas septicemia pada Ikan Jambal Siam (Pangasianodon hypophthalmus) menggunakan Larutan Kulit Nanas (Ananas comosus) Ulandari, Ulfa; Syawal, Henni; Lukistyowati, Iesje
South East Asian Aquaculture Vol. 2 No. 2 (2025): Januari
Publisher : Science, Technology, and Education Care

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61761/seaqu.2.2.68-77

Abstract

Pencegahan Motile Aeromonas Septicemia pada jambal siam (Pangasianodon hypophthalmus) dapat dilakukan dengan meningkatkan sistem kekebalan tubuh ikan dengan larutan kulit nanas (Ananas comosus). Penelitian dilakukan pada Oktober 2021-Februari 2022 di Laboratorium Penyakit Ikan dan Parasit, Departemen Akuakultur, Fakultas Perikanan dan Kelautan, Universitas Riau. Perlakuan yang digunakan adalah Kn: Kontrol negatif (tanpa perendaman dalam larutan nanas dan tidak diuji terhadap  bakteri Aeromonas hydrophila, Kp: Kontrol positif (tanpa perendaman dalam larutan nanas tetapi bakteri A. hydrophila yang ditantang, P1: perendaman ikan siam jambal dengan larutan kulit nanas pada dosis 1600 ppm dan challenge diuji dengan bakteri A. hydrophila, P2: perendaman ikan siam jambal dengan larutan nanas pada dosis 1600 ppm dan tantangan diuji dengan bakteri A. hydrophila, P3: perendaman ikan jambal siam dengan larutan nanas pada dosis 2000 ppm dan ditantang dengan  bakteri A. hydrophila. Dosis terbaik larutan kulit nanas adalah 1800 ppm, yang ditunjukkan dengan total eritrosit 252,17 x 104 sel/mm3, kadar hemoglobin 9,73 g/dL, nilai hematokrit 39,00%, sintasan 96,67% dan kualitas air selama suhu penelitian berkisar antara 27,6-28,3oC, pH 6,3-8, DO 3,42 – 4 mg/L, dan NH3 0,01-0,02 mg/L
Erythrocyte Profile of Pangasius hypophthalmus Feed with Rhizophora Apiculata Leaf Extract and Maintained in Net Cages Zissalwa, Fricilia; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol. 25 No. 1 (2020): Februari
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.70-78

Abstract

Rhizophora apiculata leaf extract has benefits as an herbal supplement that can be added to feed, because it have componds like flavonoid, tannins, saponins, terpenoids, and steroids that can improve the health of jambal fish (Pangasius hypophthalmus). This research was carried out in August to October 2019. The aim of the research was to obtain the best dose of R. apiculata leaf extract added to feed to improve the health of jambal fish as seen from total erythrocytes, hemoglobin levels, hematocrit levels, and erythrocyte morphology. The method was used an experimental method with a Completely Randomized Design (CRD) one factor of four treatment levels consisting of P0: without the addition of extracts; P1: (1.5 mg/kg); P2: (1.7 mg/kg); and P3: (1.9 mg/kg) and repeated three times so that it takes 12 units of the experiment. Maintenance is carried out for 60 days in cages net with a size of 1x1.5x1 m3 out three times, at the beginning of maintenance, 30th day, and 60th day. The best dose was in the P2 treatment (1.7 mg/kg) seen in total erythrocytes 2.57x106 cells/mm3, hemoglobin level 13.13 g/dL, hematocrit value 38.43%, absolute weight growth 38.93 g, survival rate 97,33%, and showed normal erythrocyte morphology, it was supported by water quality during the reasearch such as DO 6.4-6,9 mg/L, pH 6.1-6.8, temperature 28.2 29.50C, and ammonia 0.04-0.08  mg/L. The Blood tests were carried.
Description of Leukocytes Pangasius hypophthalmus which is Fed with Extracts of Mangrove Leaf (Rhizophora apiculata) and Maintained in The Floating Cages Rahmadona, Zikra; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol. 25 No. 1 (2020): Februari
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.79-87

Abstract

Mangrove leaves (R.apiculata) contain flavonoid compounds, terpenoids, steroids, saponins, and tannins which function as antibacterial and can improve fish health. This research was carried out in August to October 2019. This research purpose was to know the proper dose of mangrove leaf (R. apiculata) added to the feed to improve fish health as seen from total leukocytes, leukocyte differentiation, and phagocytic activity of P. hypophthalmus. The method in this research was the experimental method using the Completely Randomized Design (CRD) with one factor, four treatment levels and three times replication, so that it takes 12 units of experiments. The treatments were P0 (feed without additional extract R. apiculata), P1, P2, and P3 feed which were given extract R. apiculata at a dose of 1,5 mg/kg, 1,7 mg/kg and 1,9 mg/kg of feed. The fish used is P. hypophthalmus size 8-10 cm with a stocking density of 50 fish/m3. Feeding three times a day at 08.00, 12.00, and 17.00. Blood testing was performed three times, namely the beginning of the 1st, 30th, and 60th day of maintenance. The results showed that the addition of extract (R. apiculata) into the feed had a significant effect on total leukocytes, leukocyte differentiation, phagocytic activity, survival rate and growth of absolute weight of P. hypophthalmus. The best dose is 1,7 mg/kg of feed with total leukocytes 11,17x104 cells/mm3,  lymphocytes 84,33%, neutrophils 6,67%, monocytes 9,00%, phagocytosis activity 35,00%, survival rate 97,33%, and absolute weight growth of 38,93g. 
Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Tamba, Jaya Maruli; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.40-46

Abstract

Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Prevention of Motile Aeromonas Septiceamia in Striped Catfish (Pangasianodon hypophthalmus) using Cherry Leaf Solution Sari, Riski Mutia; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Perikanan dan Kelautan Vol. 27 No. 1 (2022): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.1.24-31

Abstract

This research was conducted in December 2019 – February 2020 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. The purpose of this study was to analyze and obtain the best dose of cherry leaf (Muntingia calabura) solution to prevent Motile Aeromonas Septicemia from total leukocytes, leukocyte differentiation, and phagocytic activity of striped catfish (Pangasianodon hypophthalmus). The method used is an experiment with a completely randomized design (CRD) one factor five levels of treatment. The doses used were negative control (without immersion cherry leaf solution and not infection with Aeromonas hydrophila), positive control (without immersion cherry leaf solution and infection with A.hydrophila), P1 (Immersion in cherry leaf solution at a dose of 2800 ppm), P2 (3,200 ppm), and P3 (3,600 ppm). Immersion was carried out 5 times for 5 minutes with an interval of 7 days. The results showed that immersion with cherry leaf solution at a dose of 3,600 ppm (P3) was the best dose (P<0.05) with a total leukocyte value of 10.66 x 104 cells/mm3, lymphocytes 84.67%, monocytes 5.67%, neutrophils 9.67%, phagocytic activity 36.67%, and survival rate 86.67%
Growth Performance of Striped Catfish (Pangasianodon hypopthalmus) Fed by Fermented Water Hyacinth Ramayani, Santi; Suharman, Indra; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol. 27 No. 3 (2022): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Fermented water hyacinth by Aspergillus niger can be used as alternative ingredient replacing soymeal for feed in striped catfish. The experimental designed consist of five treatment substitution of soymeal used fermented water hyacinth and three repliacate: T0 (feed without FWH); T1 (25% FWH); P2 ( 50% FWH); P3 (75% FWH); and P4 (100% FWH. ). A total of 375 Striped catfish weight 4.21 ± 0.42 g were stocked with a density of 25 fish m-3 in fixed net cages. The study wasconducted for 42 days. The results showed that the treatment given improved growth, feed convertion, and survival rate of striped catfish at dose FWH 50% It is recommended that substitution FWH 50% can used to replacing soymeal for feed striped catfish.