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Journal : Tropical Wetland Journal

Isolation of Bacillus thuringiensis from Type B Tidal Lands Mila, Mila; Rizali, Akhmad; Saputra, Riza Adrianoor; Sari, Yulia Padma
TROPICAL WETLAND JOURNAL Vol 10 No 1 (2024): Wetland Agricultural Issues
Publisher : Postgraduate Program - Lambung Mangkurat University (ULM Press Academic)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/twj.v10i1.126

Abstract

Bacillus thuringiensis is a disease-causing bacterium in insects that is used to control insect pests that attack crops, both crops and horticultural crops. This bacterium can be isolated from plant residues that have become compost, infected insects, and soil. Isolation was carried out to take bacteria from their natural environment and grow them in artificial media. In swamps, there are important microorganisms involved in the decomposition of organic matter. The microbes that play a role consist of fungi and bacteria, for instance, those are found in rice plantations in Barito Kuala swamps, which include the Actinomycetes, Bacillus, Cromobacterium, and Pseudomonas bacteria groups. B. thuringiensis is widely distributed in nature but is rare due to the many different Bacillus isolates. Therefore, it was necessary to conducted research to determine the presence of B. thuringiensis in plant root areas in more specific soils to increase the possibility of finding B. thuringiensis. This study aims to determine the number of B. thuringiensis isolates obtained in type B tidal land ecosystems. This research was conduct from December 2021 to June 2022 at the Integrated Laboratory and Production Laboratory, Department of Agroecotechnology, Faculty of Agriculture, University of Lambung Mangkurat. This research used a descriptive method. Soil collection was carried out by purposive sampling (sampling technique with certain considerations) in the planting area in tidal land type B. Soil samples were taken from 3 places as a comparison, namely in the rice planting area, orange planting area, and banana planting area B. The parameters observed in this study were the number of colonies, the macroscopic and microscopic shape characteristics of the bacteria, the gram of bacteria, and the test of the killing power of bacteria against P. xylostella insects. Based on the parameters that have been observed, the highest number of colonies was found in citrus plantation samples, with 98 colonies.
Co-Authors Abdul, Rahmatia R Afriyeni, Puji Afsari, Nadia Agustin, Melinda Ahmad Taqwa Ahmad, Dian Pratiwi Ainni Ainni Ajiza, Pratisa Defiera Akhmad Rizali Amin, Astuti Muh Aulia Shafa Shalsabila Awaluddin, Annisa Maharani Ayu Dyah Faradina Ayu Tiara Putri, Ayu Tiara Bidang, Azzahra Nor Afika Cut Indah Rahmadani Deva Restu Sari Dikson junus Eni Hernawati Fahira, Siti Fatmawati, Rusdiana Andika Firdaus, Amar Fitrilawatiningsih Fitrilawatiningsih Gani, Misran Helena Ch. Sipahelut Hidayat, Andi Sahrul Humairoh, Bunga Aulia Husniah Husniah Ida Ayu Putu Sri Widnyani Ihsan, Afifi Raima Indah Setiawati Indraddin Ishak, Adelia Jamilah Jamilah Kadir, Andika Saputra Karlina, Siti Kurniati, Suci Laraswati Putri Basuki Lita Lokollo M.Naufal Mubarak Mairani, Mairani Malota, Brylian Wili Marselino Marshanda, Utin Tria Marsiah, Marsiah Maulidia Hidayati, Maulidia Hidayati Meily Purnama Sari Mesi Maesaroh Mila, Nor Muhamad Hapi Muhammad Alif Muhammad Alif Muhammad Jumadin Noor Mus’idul Millah Nadila, Nadila Najwa, Fatri Nirmalasari, Ridha Noer Aisyah Barlian Noor Hujjatusnaini Nova Kayadoe Nur Ayu Fazri Nursanti, Irna Nursetyowati, Rina Olii, Sri Rahmatia M Otulawa, Siti Nur Uswatun Pramesti, Widia Pujianti Listiani Putri, Ridma Azizah Rabiah Al-Adawiyah Raden Roro Suci Nurdianti, Raden Roro Suci Rafiah Sytiawati, Rafiah Sytiawati Rahman, Andri Syaiful Rahman, Luthfi Aulia Rahmayani, Mentari Tri Indah Raihan Nadhir Ratih Pisesa Pebriyanti Reza Nadia Rifanny, Muhammad Riska Andi Rusdiana Andika Fatmawati Salim Rosyadi Sanjaya, Aditya Saka Saputra, Riza Adrianoor Sari, Yulia Padma Sasmita Sasmita Selpiana, Selpiana Siti Anafiah, Siti SITI FATIMAH Siti Maharani Siti Nur Asmah Siti Nur Asmah Siti Robeatul Adawiyah Stevi Olan Suci Kurniati Sugiarti Sugiarti Suroso Suroso Tria Syaumilla Ulumiyah, Miftahus Syifa Bahrul Victor Kayadoe Wakidul Kohar Wakidul Kohar Widyaningrum, Bakti Winri Allo Lembang Yayu Salom Yuli Tamar Filindity Yulia, Anis Nanda Yuliana Yuliana