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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Natural Science: Journal of Science and Technology Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati KUKILA JTSL (Jurnal Tanah dan Sumberdaya Lahan) Jurnal Biologi Universitas Andalas BioCONCETTA ZOO INDONESIA Jurnal Biologi Tropis JURNAL EDUCATION AND DEVELOPMENT Jurnal Kelautan Nasional AL-HAYAT: Journal of Biology and Applied Biology EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Menara Ilmu Jurnal Pembangunan Nagari Jurnal Pembelajaran dan Biologi Nukleus Sainstek : Jurnal Sains dan Teknologi Jurnal Sosiologi Andalas Jurnal Pengabdian kepada Masyarakat Nusantara Zoo Indonesia Dinamika Lingkungan Indonesia International Journal of Science and Society (IJSOC) Buletin Dharmas Andalas Biodiversitas Journal of Biological Diversity
Wilson Novarino
Andalas University
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Public Health Social Sciences Other

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In-silico Design and Testing of Tetra-Primer Arms-PCR for SNPs-Based Identification of Sumatran Tiger (Panthera tigris sumatrae): ARMS-PCR SNP Identification of Sumatran Tiger Asrori, Ikrima; Novarino, Wilson; Tjong, Djong Hon; Roesma, Dewi Imelda
Journal of Tropical Life Science Vol. 16 No. 01 (2026)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.16.01.03

Abstract

The Sumatran tiger (Panthera tigris sumatrae) is a subspecies of tiger classified as critically endangered by the International Union for Conservation of Nature (IUCN) due to poaching and illegal trade. In the law enforcement process, it is very important to ensure that the sample is indeed from a Sumatran tiger. Seized samples from illegal trade cannot be identified based on their morphology because they have been degraded and processed into other forms. Previous studies have reported the use of molecular markers for Sumatran tiger identification, which involve lengthy procedures and analyses. Therefore, for a more effective identification process, faster and more accurate techniques are needed. This study aims to design tetra primers for the identification of Sumatran tiger subspecies, particularly from incomplete confiscated samples, using the Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR) technique based on Single Nucleotide Polymorphism (SNP) markers. The primers were designed using the Primer1 application and validated in silico with CloneManager and NCBI BLAST, as well as through direct testing on Sumatran tiger blood samples. The design results showed one pair of primers with optimal specifications, producing G allele fragments (452 bp), A allele fragments (408 bp), and internal control fragments (819 bp). Specificity testing results indicated that all samples were Sumatran tigers, with amplification bands at 452 bp and 819 bp. These results demonstrate that the developed tetra-primer ARMS-PCR assay provides a rapid and practical molecular approach for identifying Sumatran tiger samples, particularly for forensic verification of confiscated wildlife materials.
Co-Authors - Fitri - Junaidi - Rizaldi . Merry Aadrean Aadrean Aadrean Abda Abda Agung Nugroho Ahmad Rizaldy Fanbudy Aini, Wardahtul Ainul Mardiah Aldino Fauzil Fanani Anas Salsabila Anas Salsabila Ani Mardiastuti Antika Fardilla Aprizal Aprizal Aprizon Putra Arba, Risqi Mutia Ardinis Arbain Arthauly Yopita Sinurat Arum Setiawan Asferi Ardiyanto Asferi Ardiyanto Asful, Ferdhinal Ashrifurrahman Ashrifurrahman Ashrifurrahman, Ashrifurrahman Asrori, Ikrima Astuti Astuti Aszareta, Muhammad Andoni Aulia Rizky Aurora, Dhea Apriano Ayu Andira AYU SMARNIA PUTRI Aziz, M. Abdul Baqi, Ahmad Iqbal Boby Hariadi Boby Hariadi Bramadi Arya Chairul Cynthia Ericca Dahelmi Dahelmi Damanhuri, Harfiandri Dedi Hermon Dewi Chandrarini Surya Dewi Imelda Roesma Diana Sari Diana Sari Diana Sari Djong Hon Tjong DYAH PERWITASARI-FARAJALLAH Efrita Ruswenti Efrizal Efrizal Elsa Yolarita Erik Marlius Erizal Mukhtar Eti Yerizel fachrul reza, fachrul Fadil, Muhammad Syukri Fadillah Fardilla, Antika Farid Azel Fauri Yatul Irfan Yanti FAUZIAH SYAMSI Fauziah Syamsi Fikri, Husnul Fikriya - Rahma Firjatullah, Fadhilah Firly Widuri Gita Herliza Sari Hadi Addaha, Hadi Hadi Kurniawan Hamdi Ibrahim, Muhammad Hanif Fadly Helmi Helmi Hendra, Jon hendrita, juli Henny Herwina Hiroshi Kobayashi Idris, Muhamma IKRIMA ASRORI IKRIMA ASRORI Ilham Samudra, Muhammad Inda Dwi Solina Inda Dwi Solina Indang Dewata Indra Junaidi Zakaria Indra Junaidi Zakaria, Indra Irvan Fadli Wanda Jabang Nurdin Janra, M. Nazri Janra, Muhammad Nazri Jarulis . Jarulis Jarulis Jarulis Jarulis Kamilah, Santi N Karmelia Kontesa Kevin Origia Kevin Origia Khairini, Ridha Kurniadi Ilham Laksono Trisnantoro Lilik B. Prasetyo Lilik B. Prasetyo Lintang Yodhy M. Nazri Janra M. Silmi M. Syafrie M.Nazri Janra Mahdi Mahdi Mahdi Mahdi Mahfud Huda Maliza, Rita Mansyurdin Marchellino Irwan, Reziq Muhammad Afif MUHAMMAD AZHARI AKBAR Muhammad Idris Muhammad Idris Muhammad Silmi Mukhta, Erizal Nazri Janra, Muhammad Nindy Ladyfandela Nofrita Nofrita Nofrita Nofrita Nova Adri Yanti Novita, Aulya Nur Halimah Rangkuti Nur Insani PATRICK FLAGGELLATA Pratama Elisa, Tasya Putri Pratama, Raihan Anugrah Putra Santoso PUTRI LISYA ANGGRAINI Putri Pratama Elisa, Tasya Putri, Syntia Mai Rahmi Fitri Ratna Juwita T Reviany Widjakusuma Reziq Marchellino Irwan Richard Noske Richard Noske Ridho, Muhammad Syifa'ur Rifta Septiavi Risqi Mutia Arba Risqi Mutia Arba Rizaldi Rizaldi Rizaldi Rizaldi Rizaldi, . Rizaldi, Rizaldi Rizka Sefmaliza Robby Jannatan RUSDIYAN RITONGA Rusdiyan p. Ritonga Santi N Kamilah SARUEDI SIMAMORA Semeidi Husrin Silmi, M. Sinurat, Arthauly Yopita Sugeng Santoso Suparno . Syafrie, M. SYAIFULLAH SYAIFULLAH SYAIFULLAH SYAIFULLAH SYAIFULLAH SYAIFULLAH Syaifullah Syaifullah Tasya Putri Pratama Elisa Taufiq Afdhal Tjong, Tjong Hon Tofrizal, Alimuddin Tomi Kasayev Try Al Tanto Uswatul Hasana Utami, Radila Widjakusuma, Reviany Widodo, Febri Anggriawan YAMATO TSUJI Yeni A. Mulyani YOLI ZULFANEDI Yunila Berliana Yusni Handayani Zaini Zaini Zettyra Sandova