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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Biotechnology BIO-SITE |BIOLOGI Sains Terapan BIOPROSPEK Indonesian Journal of Chemistry Communications in Science and Technology Biosaintifika: Journal of Biology & Biology Education Berkala Penelitian Hayati Biosaintifika: Journal of Biology & Biology Education Jurnal Ilmiah Ilmu Kesehatan
L. Hartanto Nugroho
Fakultas Biologi Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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POTENSI EKSTRAK ORGAN VEGETATIF ANGGREK VANDA HASIL PERSILANGAN SEBAGAI AGEN ANTIKANKER Maturbongs, Agatha; Pratiwi, Rarastoeti; Nugroho, L. Hartanto; Maturbongs, Rudi A.
Jurnal Ilmiah Ilmu Kesehatan Vol. 1 No. 2 (2023): Volume 1 Nomor 2 Tahun 2023
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jiik.v1i2.12908

Abstract

Penelitian ini bertujuan untuk menguji sitotoksisitas fraksi ekstrak kloroform gabungan organ vegetatif Vanda hasil persilangan terhadap kanker payudara cell line T47D dan kanker serviks cell line HeLa. Organ vegetatif dari tanaman tersebut diekstrak dan difraksinasi. Hasil fraksinasi dengan profil yang sama digabungkan. Fraksi gabungan diuji sitotoksisitasnya terhadap cell line T47D dan cell line HeLa menggunakan metode MTT Assay. Analisis data untuk penentuan IC50 dihitung dengan analisis probit dalam SPSS 17. Hasil penelitian menunjukan fraksi C yang paling baik dalam menghambat pertumbuhan sel kanker payudara cell line T47D dan sel kanker serviks cell line HeLa.
Antioxidant and Anticancer Activity of Dillenia serrata Thunb Ethanol Extract Against MCF-7 Breast Cancer Cell Line Rahmawati, Rahmawati; Zulkifli, Zulkifli; Nuringtyas, Tri Rini; Jenie, Riris Istighfari; Nugroho, Laurentius Hartanto
Indonesian Journal of Cancer Chemoprevention Vol 13, No 3 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss3pp175-183

Abstract

Women’s breast cancer incidence rate in Indonesia ranks number one with 12 per 100,000 cases, with luminal A as the dominant subtype. Currently, chemotherapeutic agents have limitations that lead to inefficiencies in therapy, therefore it is necessary to develop more effective and efficient chemopreventive agents. Plant secondary metabolites can provide pharmacological effects that can be used as chemoprevention agents. Secondary metabolites of D. serrata may have pharmacological effects as antioxidants and cytotoxic. This study aims to determine the antioxidant properties and cytotoxic activity of D. serrata ethanolic extract on the MCF-7 breast cancer cell line. The leaves of D. serrata were macerated, while the bark and root samples were refluxed with 96% ethanol as solvent. All extracts were evaporated with a rotary evaporator. Qualitative evaluation of the phytochemical content of leaf ethanolic extract, bark ethanolic extract, and root ethanolic extract was done using the standard tube test method. The antioxidant assay was carried out using the DPPH. The cytotoxic activity was determined in vitro using an MTT assay against the MCF-7 cell line with a series of concentrations from 12.5–400 μg/mL. Doxorubicin was the positive control treated at a 3.125–100 μg/mL concentration. The antioxidant activity showed that leaf extract had the highest antioxidant activity, followed by root and bark extract, with IC50 values of 95.66, 270.5, and 335.96 ppm, respectively. Leaf ethanolic extract and root ethanolic extract’s cytotoxic ability is considered moderate cytotoxic with IC50 values of 493.17 and 229.82 μg/mL, respectively. Amongst the ethanolic extract from the leaf, bark, and root of D. serrata, the leaf ethanolic extract has the best anti-oxidant activity and the bark ethanolic extract was the most cytotoxic one against MCF-7 cells.Keywords: Antioxidant, Cytotoxic, Dillenia serrata, MCF-7.
The Phytochemical and Biological Activities of Two Phyllanthus Species: Insights into Metabolit, Antioxidant and Antibacterial Activity Araújo, Otilda Fátima; Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Nugroho, Laurentius Hartanto
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.13944

Abstract

Phylanthus species, including P. niruri and P. urinaria have pharmacological potential due to their rich phytochemical composition. People usually used this plant for medicinal treatments. This study aimed to compare the phytochemical and antioxidant and antibacterial activities of two Phyllanthus species. The whole of plants was extracted using maceration method with ethanol as solvent. Phytochemical content analyzed using spectrophotometer.  The reagent used for each compounds that were Folin-ciocalteu for phenolics, AlCl3 for flavonoid, dimethyl sulfoxide for chlorophyll and carotenoid. Bioactivity analysis using 1,1-diphenyl-2-picrylhydrazyl method for antioxidant activity and Kirby-Bauer method for antibacterial activity. The highest flavonoid content (12.22 mg QE/gram extract) and total chlorophyll (43.2 µg/ml extract) in P. niruri while phenolic content (80.8 mg GAE/gram extract) in P. urinaria. The carotene of both Phyllanthus were similar (11.9 µg/ml extract. The IC50 values of P. urinaria (6.16 ± 0.42 μg/ml) and P. niruri (17.72 ± 0.80 μg/ml), which indicated very strong antioxidant activity. P. urinaria leaf extract had stronger inhibition against Escherichia coli than Staphylococcus aureus (>20 mm) and P. niruri leaf extract could inhibit E. coli and S. aureus bacteria at all concentrations (11-20 mm). This study found that phenolic compounds strongly influenced the antioxidant and antibacterial abilities of Phyllanthus, while chlorophyll and carotenoids had only a slight influence. These findings open up opportunities to utilize P. niruri and P. urinaria as antioxidant and antibacterial agents.
Pengaruh Konsentrasi Sukrosa terhadap Kadar Piperin pada Kalus Cabe Jawa (Piper retrofractum Vahl.) Dewi, Kartika Puspita; Nugroho, Laurentius Hartanto; Sasongko, Aries Bagus; Hidayati, Lisna
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2023): June 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i2.6347

Abstract

Cabe jawa (Piper retrofractum Vahl.) merupakan tanaman herbal yang mengandung alkaloid utama piperin. Prospek cabe jawa sebagai bahan obat belum didukung oleh ketersediaan bahan baku yang cukup, karena rendahnya produktivitas tanaman. Salah satu alternatif untuk mengatasi permasalahan tersebut adalah dengan menerapkan kultur kalus. Kalus dapat memproduksi metabolit sekunder relatif cepat dan berkelanjutan. Efektivitas produksi metabolit sekunder seperti piperin pada kalus dapat dilakukan dengan meningkatkan konsentrasi sukrosa dalam medium kultur. Penelitian ini dilakukan untuk mengetahui pengaruh konsentrasi sukrosa pada morfologi kalus, pertumbuhan kalus, dan produksi piperin pada kalus cabe jawa. Medium Murashige and Skoog (MS), dan zat pengatur tumbuh naphtalene asetic acid (NAA) dan benzyl aminopurin (BAP) 1:2 digunakan untuk menginduksi kalus dari daun cabe jawa. Kalus yang terbentuk berwarna hijau muda dan kompak. Kalus disubkultur selama 35 hari pada medium MS dengan konsentrasi sukrosa 30 g/L, 40 g/L, 50 g/L, dan 60 g/L. Setelah inkubasi warna kalus berubah dengan tekstur tetap kompak. Berat segar dan berat kering dari kalus menurun dengan bertambahnya konsentrasi sukrosa. Piperin pada kalus diekstraksi dengan etanol 96% dan diukur kadarnya dengan Kromatografi Lapis Tipis (KLT)-Densitometri. Peningkatan konsentrasi sukrosa pada medium kulur tidak berpengaruh pada kadar piperin dari kalus cabe jawa. 
Co-Authors Aditya Krishar Karim Araujo, Otilda Fatima Ari Indrianto Aries Bagus Sasongko, Aries Bagus Dewi, Kartika Puspita Elizabeth Betty Elok Kristiani Elly Rustanti Evi Mintowati Kuntorini Kumiko Terada Linus Yhani Chrystomo Lisna Hidayati, Lisna Ma'rufah, Hastini Maria Dewi Astuti Maturbongs, Agatha Maturbongs, Rudi A. Pudji Astuti Rahmawati, Rahmawati Rarastoeti Pratiwi Retno Aryani Riris Istighfari Jenie Rosyida Azis Rizki Santosa Santosa Soekarti Moeljopawiro Sri Kasmiyati Stalis Norma Ethica Subagus Wahyuono Sukarti Moeljopawiro Sukirno Sukirno Tri Joko Raharjo Tri Rini Nuringtyas Tsutomu Nohno Yulita Nurchayati Yus Hargono Cahyaning Yudi Zulkifli Zulkifli