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Journal : Indonesian Journal of Biotechnology

Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10 Nur Arfa Yanti; Langkah Sembiring; Sebastian Margino
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.322 KB) | DOI: 10.22146/ijbiotech.7804

Abstract

A new bacterial strain that produces amylase and poly-a-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics  and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by  Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Nur Arfa Yanti; Langkah Sembiring; Sebastian Margino; Nurhayani H. Muhiddin
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.882 KB) | DOI: 10.22146/ijbiotech.7877

Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB. Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
Co-Authors A. Nurhana Abdul Mun'im Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Alfin Alfin Amirullah -, Amirullah Andi Septiana Andi Tenri Ampa Ardiansyah ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Ardiansyah Ardiansyah Asmawati Munir, Asmawati Asrul Sani Aswan, Muhammad Aswan Bakkareng, Wirdhana Budiman, Herdi Cahyanti, Kartika Dwi Dedy Oetama Desty Tryaswaty DIRVAMENA BOER, DIRVAMENA Erni Martani Fahyuddin Fahyuddin, Fahyuddin Fatimah Alwi Albakar Haidin Haidin Haji Muhiddin, Nurhayani Handayani Hamid, Fitri Ida Usman Ida Usman Indrawati Indrawati Irnawati Irnawati Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamili Jamili Karya, Adi Kurnia Yati La Ode Adi Parman Rudia La Ode Ahmad Nur Ramadhan La Ode Iman Sulaiman La Ode Kadidae, La Ode Langkah Sembiring Lili Darlian, Lili M. Jahiding M. Rajab Sutra Mijaya Marlina, Wa Ode Leni Marwati Arji Mashuni Mashuni Mashuni Pallawagau Muhammad Jahiding Muhiddin, Nurhayani Haji Muhsin Muhsin Muhsin Muhsin Muhsin Muhsin Muksar, Muksar Muzuni, Muzuni Nurhayani H. Muhiddin Nurhayani H. Muhiddin, Nurhayani H. Nurhayani Haji Muhiddin Prasetya, Wandy Murti Ramlawati, Ramlawati Rudia, La Ode Adi Parman Santi Septiana, Santi Sebastian Margino Sebastian Margino Sitti Wirdhana Ahmad Sri Ambardini Sri Ambardini Sri Noegrohati Sulaiman, La Ode Iman Suleman, Darwis Surachma, Wahyuni Nurul Suriana Suriana Suriana Suriana Suriana Suriana Syah, Muhamad Azwar Taufik Walhidayah Vidya Nur Riska Parakkasi Wa Ode Isra Wa Ode Nanang Trisna Dewi Wa Ode Sitti Nurhaliza Wahyu Ahwarul Asis Wina Rezky