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The Effectiveness of α-Mangostin in Reducing the Streptococcus Mutans Biofilm Thickness Rahmawati Sri Praptiningsih; Arlina Nurhapsari; Rifqi Wahyu Febrian
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 11, No 2 (2022): November
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v11i2.15809

Abstract

Dental caries occurs due to the demineralization of tooth structure caused by microorganisms in colonies called biofilms. One of the microorganisms involved in dental caries is Streptococcus mutans. Oral mouthwash, in addition to mechanical cleaning, is known to prevent the growth of oral microorganisms. Mangosteen is known as an anti-cancer ingredient with high anti-bacterial properties. This study aims to identify the effectiveness of mangosteen skin extract (Garcinia mangostana L.) in decreasing Streptococcus mutans biofilm. The study is experimental research with a post-test control group design. The research sample was divided into five groups; 3 treatment groups and 2 control groups. The biofilm thickness test was carried out with OD (Optical density) with a wavelength of about 620 nm using an ELISA reader. Kruskal Wallis analysis was employed as a non-parametric statistical test analysis. Statistical Kruskal Wallis indicated significant differences in the thickness of 5 test groups of Streptococcus mutans. The lowest average yield of biofilm thickness was in the α-mangosteen group at 12.5 g/ml. Conclusion: α-mangosteen in mangosteen skin extract effectively reduced the thickness of Streptococcus mutans biofilm.
EFFECTIVENESS OF THREE INTRACANAL MEDICAMENTS AGAINST STAPHYLOCOCCUS AUREUS Yunita Styaningrum; Arlina Nurhapsari; Denis Yusfa
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.89-94

Abstract

Background: Medikamen intrakanal used to remove remaining bacteria in the root canal after the instrumentation process, especially with some of the material between the infected root canal. The purpose of the medikamen intrakanal, among others, is reducing the number of bacteria, by preventing the growth of bacteria, eliminates exudate periapikal, prevent or restrain resorpsi root and prevent infected root canal system. The most influential of these bacteria i.e. Staphylococcus aureus. The purpose of this study was to determine the effectiveness of three intracanal medicament ingredients as anti-bacterial in Staphylococcus aureus.Method: The research, an experimental laboratory method carried out with a post test only control group design, was performed on Staphylococcus aureus bacteria that had been cultured and divided into 4 groups each given 1 mg zinc oxide paste, 1 mg antibiotic paste, 1 mg calcium hydroxide, and aquadest as negative control. Subsequently the results are seen from the zone of inhibition and kill zone which are measured by calliper and analyzed by One-way Annova followed by the Post-hoc LSD.Result: The result of On-way Anova test (p=0.000), showed there was a difference for using medicaments zinc oxyde, antibiotic paste, and calcium hydroxide towards Staphylococcus aureus. The post hoc LSD test showed there was a significant mean’sdefference of inhibition zone and kill zone (p-0.05) intergrups.Conclusion: there was an effect after using zinc oxyde, antibiotic paste, and calcium hydrxide as antibacterial on Staphylococcus aureus bacteria seen from inhibition zone and kill zone.
THE METAL BRACKETS STAINLESS STEEL SURFACE ANALYSIS OF THE NICKEL (Ni) AND MANGAN (Mn) ION RELEASE Grahita Aditya; Arlina Nurhapsari; Balqis Nureska Khomsiana
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.68-71

Abstract

Background: The installation of stainless steel metal brackets on the patient's teeth in a long phase will interact with the oral cavity environment which causes the release of ions such as Ni and Mn ions. Their release in the metal bracket can impact the body in the form of hypersensitivity reactions while the impact on the bracket is the bracket surface changes shown by the gap in it. These changes can reduce the effectiveness of orthodontic and aesthetics treatments, quality and the strength of the bracket, and can provide an attachment place for Streptococcus mutans microbe. The aim of this study was to determine the release of the ions (Ni and Mn) on the stainless steel metal bracket surface.Method: This study was a descriptive research. The research sample used a stainless steel metal bracket with 0.22" lower jaw premolar slots which was marked by the American Orthodontic (AO) brand which experienced the ions release. The observation of ion Ni, Mn, Fe and Cr release on the bracket was carried out by using a Scanning Electron Microscope- Energy Dyspersive X-Ray Spectroscopy’s tool (SEM-EDX).Result: The results showed that the mean percentage of Ni ions decreased by 0.758 % while the mean percentage of Mn ions decreased by 0.324 %. The percentage of ion release is calculated from all ion Ni, Mn, Fe and Cr.Conclusion: The results can be concluded that there were differences of ion release` (Ni and Mn) on the stainless steel metal bracket surface. Their release was demonstrated by the presence of microscopic gaps due to pitting corrosion.
COMPARISON OF THE NUMBER OF MACROPHAGES IN THE RAT REVERSIBLE PULPITIS AFTER APPLICATION OF BIODENTIN AND SIWAK EXTRACT Andina Rizkia Putri Kusuma; Rahmawati Sri Praptiningsih; Arlina Nurhapsari
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.6-11

Abstract

Background: Oral disease still need special attention, especially in dentistry. Inflammation of the pulp is caused by the presence of irritants. One of the mechanical irritants is iatrogenic factors caused by operator error during preparation so that roof of pulp chamber is exposed. This condition is called reversible pulpitis/inflammation of the pulp. Cells that play a role during inflammation are macrophages which are the second defense cell after neutrophil apoptosis. A direct pulp capping procedure is indicated for this condition. The material commonly used is biodentin, but because the price tends to be expensive, alternative materials are needed. Siwak contains flavonoids which are known to play an important anti-inflammatory role in the healing of exposed pulp. The purpose of this study was to compare the number of macrophages in the dental pulp of rats with reversible pulpitis after administration of biodentin and siwak extract.Method: This research is a true experimental with a post test only group design, consisting of 2 treatment groups, namely the 75% siwak extract group and the biodentin group. The material is applied after the tooth has been prepared. The research sample used male wistar rats and sacrificed on the 3rd day. Samples were stained with Hematoxylin Eosin to see macrophage cells.Result: The data obtained were analyzed using the independent T-test. The test results showed a significant difference, with a value of p = 0.008 (p <0.05).Conclusion: The conclusion of this study that the siwak extract group showed a lower mean number of macrophage cells than the biodentin group.
EFFECT OF ASIATIC ACID ISOLATE ON DECREASING REACTIVE OXYGEN SPECIES (ROS) LEVELS IN MICE REVERSIBLE PULPITIS shella indri novianty; Arlina Nurhapsari; Anitalia Sukma Permata Sari
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.56-62

Abstract

Background: Pulpitis is a form of pulp inflammation that occurs due to the response of vascular connective tissue to a trauma or continuation of caries. During inflammation the release of Tumor Necrosis Factor Alpha (TNF-) will regulate excess ROS. Reactive Oxygen Species (ROS) can damage cellular lipids that cause oxidative stress, cell or tissue damage in mice caused by ROS to be seen with malondhydehyde biomarker (MDA) donors. Asiatic acid is a natural antioxidant that is able to neutralize free radicals by suppressing oxidative stress. This study aims to determine the effect of asiatic acid isolates on the reduction of ROS levels in reversible pulpitis mouse models.Method: This type of research is an experimental laboratory in vivo, conducted by making pulpitis on the right and left teeth of the right and left jaws of 24 male Wistar rats who were treated in the form of a control group, Asiatic Acid 0.5%, 1% and 2%, then examined using elisa sandwich method Result: The One way anova test showed that the highest mean rank was the control group and the lowest was the Asiatic Acid group of 1%. The p value < 0.05, there was a significant difference in the concentration of Asiatic Acid isolates against the significant reduction in ROS levels in reversible pulpitis between the four groups. Post Hoc LSD (Least Significant Difference test in between control groups and 1%, 0.5% and 1% group, and 1% and 2% groups obtained p < 0.05 which means there is a significant difference in reactive oxygen species (ROS) levels in reversible pulpitis between the two groups.Conclusion: Asiatic Acid was able to reduce the amount of Reactive Oxygen Species (ROS) as seen from the parameters of Malondialdehyde antibodies (MDA) in male wistar mice that developed reversible pulpitis
PENATALAKSANAAN GIGI PREMOLAR KEDUA MAKSILA DENGAN SALURAN AKAR VERTUCCI TIPE V - laporan Kasus Arlina Nurhapsari
Odonto : Dental Journal Vol 1, No 1 (2014): July 2014
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.072 KB) | DOI: 10.30659/odj.1.1.53-56

Abstract

Background: The aim of the case report is to demonstrate the importance of knowing the root canals configuration in the maxillary second premolars. Maxillary second premolars usually have one root canal, however in several cases there is more than one root canal with a variety of configurations. Method: multiple visit root canal treatment with a crown down technique on the maxillary second premolar with unusual root canals configuration which is confirmed using radiograph. Result: Based on radiograph, it was identified Vertucci type V root canal on the maxillary second premolars. After biomechanical preparation, obturation was conducted. When patient controlled, there were not problem and inflammation. Conclusion : This report described and discussed about the possibility of root canals variation on the maxillary second premolars. Careful examination using radiograph and deep knowledge, it is identified by the clinician in treating root canal treatment on that premolars.
PERBANDINGAN KEBOCORAN TEPI ANTARA RESTORASI RESIN KOMPOSIT TIPE BULK-FILL DAN TIPE PACKABLE DENGAN PENGGUNAAN SISTEM ADHESIF TOTAL ETCH DAN SELF ETCH Arlina Nurhapsari
Odonto : Dental Journal Vol 3, No 1 (2016): July 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.436 KB) | DOI: 10.30659/odj.3.1.8-13

Abstract

Background: Composite resin has been widely used because of it's great aesthetic and physico-chemical properties. Type of posterior composite resins mostly used nowadays are packable and bulk-fill composite resins. Shrinkage in composite resin resulting microleakage that could lead to pulp sensitivity. Dentin bonding adhesive system can help to minimalize shrinkage. There are two type of adhesive system, the total etch and self etch group. The purpose of this study was to compare the microleakage between bulk-fill and packable composit resin with two different types of dentin bonding adhesive system. Method: twenty premolar teeth used as sample. The teeth were divided into four groups of 5 teeth each and Class I cavity preparations were prepared. The groups are : G1 (PC + TE), G2 (PC + SE), G3 (BC + TE), G4 (BC + SE). The specimens were thermocycled, stained with methylene blue dye, and sectioned to evaluate the dye penetration.Result: Kruskal Wallis test showed no difference among 4 groups. Meanwhile, Mann Whitney test showed significance difference between G2 - G3, and G3 - G4.Conclus/on: The study demonstrated that total etch has better sealing ability than self etch and there was no significant microleakage difference between bulkfill composite and packable composite.
PENGARUH STIMULASI PERMAINAN ULAR TANGGA TENTANG GINGIVITIS TERHADAP PENGETAHUAN ANAK USIA 8-11 TAHUN Studi terhadapSiswa SD Negeri Kuningan 04, Kecamatan Semarang Utara Syarifah Nur Laili Siyam; Arlina Nurhapsari; Benni Benyamin
Odonto : Dental Journal Vol 2, No 1 (2015): July 2015
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.906 KB) | DOI: 10.30659/odj.2.1.25-28

Abstract

Background: Health education was an attempt to increase the knowledge of children to health problems. The provision of health education can be done through stimulation using Educational Educative Equipment (APE) form snakes and ladders game . One of the health problems that was unfamiliar to the child that is gingivitis. Purpose: This study was conducted to determine the effect of stimulation of gingivitis snakes and ladders game against the knowledge of children ages 8-11 years. Method: This research method using descriptive analytic with pre and post test design. Samples were collected using simple random sampling method comprising 102 respondents based on inclusion criteria of the SD N Kuningan 04. The independent variable is the stimulation of snakes and ladders game. The dependent variable is the child's knowledge about gingivitis. Data were collected using a questionnaire and analyzed the Wilcoxon Signed Rank test. Result: Based on the Wilcoxon Signed Rank test showed significance value 0.00 (p <0.05), so it can be concluded that health education using stimulation of snakes and ladders game has the effect to increase the knowledge of gingivitis in children. Conclusion: From these results it can be concluded that education using the stimulation of snakes and ladders game effect to increase children's knowledge about gingivitis.
LEVELS OF MALONDIALDEHYDE AND CALCITONIN GENE-RELATED PEPTIDE IN PULP INFLAMMATION DUE TO LPS INDUCTION DURATION Arlina Nurhapsari; Andina Rizkia Putri Kusuma
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.175 KB) | DOI: 10.30659/odj.9.1.148-152

Abstract

Background: Administration Lipopolysaccharide (LPS) to pulp tissue might produce inflammation. Inflammation occurs, which can lead to oxidative stress and neuropeptides release. Malondialdehyde (MDA) is an oxidative stress measure, while calcitonin gene-related peptide (CGRP) is a neuropeptide found in large amounts in the pulp. This study attempted to analyze levels of CGRP and MDA in LPS inflamed rat pulp tissueMethode: Wistar rats' maxillary incisors were treated to LPS stimulation. A total of 30 mice were separated into six groups, each with five animals. The groups were created by administering LPS to the pulp for 6, 12, 24, 48, and 72 hours. Rat without pulpal exposure (0 hour) served as controls. Enzyme-linked Immunosorbent Assay (ELISA) was used to measure the levels of MDA and CGRP in the pulp tissue.Result : MDA and CGRP levels were found to be significantly different in all groups (p0.05 mohon diberi keterangan apakah </>/=). With LPS administration, MDA levels increased on average, whereas CGRP levels fluctuated. Conclusion: The presence of inflammation in the pulp was shown by changes in MDA and CGRP levels after 6 hours of LPS injection. The effect of rising MDA levels on inflamed pulp tissue did not produce effects that were directly proportional to or vice versa with CGRP levels that showed fluctuated.
PENYERAPAN AIR DAN KELARUTAN RESIN KOMPOSIT TIPE MICROHYBRID, NANOHYBRID, PACKABLE DALAM CAIRAN ASAM Arlina Nurhapsari; Andina Rizkia Putri Kusuma
Odonto : Dental Journal Vol 5, No 1 (2018): July 2018
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (791.636 KB) | DOI: 10.30659/odj.5.1.67-75

Abstract

Background: Composite resin is the most widely used restoration material. This material is composed of several materials such as polymer matrix, fller particles and silane coupling agents. As technology progresses, microhybrid, nanohybrid and packable composite resin materials are developed, with good handling and mechanical properties. As a polymer-based material, the composite resin absorbs the liquid, causing the solubility of the resin matrix. Carbonated drinks and chlorine, are acidic liquids that can cause soluble resin matrix. The aim of this paper is to compare water sorption and solubility between composite resin type microhybrid, nanohybrid, packable from different brand.Methods: This research used experimental method with sample of composite resin disk with diameter 15 mm and thickness 1 mm. The number of samples was 90 divided into 18 treatment groups, 9 group immersed in carbonated drinks and 9 group immersed in chlorine. The grouping is based on composite resin type microhybrid, nanohybrid, packable from different brand. Water sorption is measured by the sample mass difference before and after immersion then divided by the initial volume of the sample. While the solubility of the composite resin was measured by reducing the sample mass prior to submersion and afterdrying then divided by the initial volume of the sample.Result: The results showed signifcant differences in water sorption on chlorine, solubility in carbonated beverages and chlorine. Meanwhile, an insignifcant difference occurred in water sorption in carbonated beverages.Conclusion: water sorption and solubility in 3 types of composite resin with9 different product, not depend on the composite resin type but depend on the product used