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All Journal IPTEK The Journal for Technology and Science Prosiding Seminar Biologi Biota Journal of Health Sciences JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Journal of Multidisciplinary Applied Natural Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Sewagati
Awik Puji Dyah Nurhayati
Institut Teknologi Sepuluh Nopember
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Journal : Journal of Multidisciplinary Applied Natural Science

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Antioxidant and Anticancer Effects of Red Okra (Abelmoschus esculentus L.) Ethanol Extract through In Vitro and In Vivo Colorectal Cancer Models Wahyuningsih, Sri Puji Astuti; Mamuaya, Brigita Klara Krisdina; Dewi, Firli Rahmah Primula; Hapsari, Lukiteswari Dyah Tri; Kusuma, Baskara Wiku Adi; Nurhayati, Awik Puji Dyah
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.367

Abstract

Colorectal cancer (CRC) remains the second leading cause of cancer-related mortality worldwide. Affordable herbal sources such as red okra (Abelmoschus esculentus L.) pods have gained attention as potential alternative therapies for CRC. This study aimed to evaluate the antioxidant and anticancer effects of red okra ethanol extract (ROE) using both in vitro and in vivo colorectal cancer models. The antioxidant activity of ROE was assessed using the DPPH assay, while cytotoxic activity was evaluated using the MTT assay on SW480 and HCT116 cell lines. An in vivo study was conducted using rats divided into six groups: normal control, negative control (MNU 10 mg/kg BW), positive control (MNU + methotrexate 0.08 mg/kg BW), and treatment groups receiving MNU combined with ROE at doses of 50, 100, and 200 mg/kg BW for 28 days. Serum levels of Bcl-2, COX-2, VEGF, and MMP-9 were analyzed, and histopathological evaluations of colon tissues were performed. Data were statistically analyzed using one-way ANOVA followed by Duncan’s post hoc test. Statistical significance was determined at p<0.05. ROE exhibited potent antioxidant activity (IC₅₀ = 59.66 ppm) and induced cytotoxic effects by reducing SW480 cell growth and inhibiting HCT116 cell proliferation. Moreover, ROE significantly decreased the expression of Bcl-2, COX-2, VEGF, and MMP-9. These biomarkers are associated with apoptosis inhibition, angiogenesis, inflammation, and metastasis, respectively. Histopathological analysis confirming reduced inflammatory infiltration and suppression of colon carcinogenesis. The optimal in vivo dose was 50 mg/kg BW. These findings support the development of ROE as a promising natural agent for colorectal cancer prevention and therapy.
Co-Authors Adik Mayang Indiani Adik Mayang Indiani Afrilya Dina Salsabila Agus Wahyudi Agus Wahyudi Alfani Raziful Dwi Ersandy Alifia Cahyaning Putri Anak Agung Gede Sugianthara Anjar Tri Wibowo Annisya Fitri Rachmada Ary Bachtiar Krishna Putra Aulia Istighfarotunnisa Bagastyo, Arseto Yekti BUDI SETIADI DARYONO Danang Samudra Devi Pramesti Wulandari Dewi Hidayati Didit Prasetyo Dini Adni Navastara, Dini Adni Dini Ermavitalini Edwin Setiawan Elshinta Riantica Firli Rahmah Primula Dewi Fitri Lianingsih Geoloveriana Esasi Hapsari, Lukiteswari Dyah Tri IDAA Warmadewanthi Istriyati Istriyati Kamila Islamiati Kharisma Armida Ulayya Kristianto Kristianto Kusuma, Baskara Wiku Adi Kuswytasari, Nengah Dwianita Latifah, Vida Rahma Lissa Rosdiana Lissa Rosdiana Maitsa Adzro Fania Mamuaya, Brigita Klara Krisdina Meitha, Karlia Miftahudin . Millah, Ro’iqotul Nadhila, Auliya Rahma Nadila Wulan Cahyani Natanael Hari Wijaya Ni Wayan Ni Wayan Nichole J de Voogt Noor Nailis Sa'adah Noor Nailis Saadah Noor Nailis Saa’dah Noor Nailis Sa’adah Nova Maulidina Ashari Nova Maulidina Ashuri Nugroho, Giri Nurlita Abdulgani Nurlita Abdulgani Nurul Jadid Putro, Karso Suryo Rahmadani, Syakirah Shafwah Rarastoeti Prastiwi Rarastoeti Pratiwi Ratna Rintaningrum riantica, elshinta Rony Irawanto Sa'adah, Noor Nailis Saptarini, Dian Sari, Noorkomala Sartika, Dian Sa’adah, Noor Nailis Shabrina Syifa Ghaissani Shabrina Syifa Ghaissani Shovitri, Maya Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Subagus Wahyuono Sukardiman Sukma, Nur Awaliyah Mentari TATIK CHIKMAWATI Triono Bagus Saputro, Triono Bagus Welly Herumurti Wijayanti Pujitono Yusuf, Adib Fakhruddin Zufar Azzaria Azmi Zuwanita, Cyntya Sri