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All Journal JPMS (Jurnal Pendidikan Matematika dan Sains) Jurnal Penelitian Saintek Jurnal Penelitian Humaniora INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Ganendra: Majalah IPTEK Nuklir Jurnal Sains Dasar Biotropika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Journal of Education Technology Jurnal Abdi Insani Borobudur Jurnal Natural Kingdom (The Journal of Biological Studies) IJOBI Jurnal Pendidikan Progresif
Bernadetta Octavia
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Neuroscience Physics Public Health Social Sciences Other

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Journal : IJOBI

 1 
MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

Abstract

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
Antibacterial Activity of Green Meniran Extract (Phyllanthus niruri Linn) on The Growth of Salmonella typhimurium Nafisah, Aulia Erta; Octavia, Bernadetta; Lestari, Endang Gati
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 2 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i2.215

Abstract

Salmonella typhimuriumis a bacterium that causes digestive infections, gastroenteritis, and food poisoning, psickcaused byBacterial infection is a health problem in developing countries, including Indonesia. Green meniran (Phyllanthus niruri L.), is a herbaceous plant containing flavonoids, alkaloids, saponins, and tannins which have antibacterial activity which is expected to suppress the development of Salmonella sp. The aim of the study was to determine the ability of green meniran extract as an antibacterial to inhibit the growth of Salmonella typhimurium bacteria. The study used a completely randomized factorial design consisting of two factors, namely extract concentrations of 5%, 10%, 20%, 40%, 80%, equipped with a positive control (chloramphenicol) and negative control (aquades), as well as the age of the inoculum of the bacterial growth phase. Testing the antibacterial activity using the Kirby-Bauer disc diffusion method. The results showed that green meniran extract had antibacterial activity against the growth of Salmonella typhimurium bacteria. Concentrations of 5%, 10%, 20%, 40%, and 80% produced inhibition zone diameters of 6.7 mm, 8.5 mm, 9.9 mm, 12 mm, and 14.6 mm, respectively. The concentration of 80% green meniran extract is effective in inhibiting the growth of Salmonella typhimurium bacteria with an antibacterial effectiveness value of 58.95%.
INHIBITION OF MANGKOKAN LEAF (Nothopanax scutellarium Merr.) ETHANOL EXTRACT ON THE GROWTH OF Salmonella typhimurium BACTERIA Sari, Herninda Dhama; Octavia, Bernadetta
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 1 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i1.747

Abstract

Typhoid fever is an infectious disease of the digestive tract caused by the bacterium Salmonella typhimurium. Mangkukan leaf (Nothopanax scutellarin Merr.) can be an alternative treatment because it contains flavonoids, saponins, tannins, and alkaloids. This study aimed to determine the ability of Mangkukan leaf extract as an antibacterial in inhibiting the growth of S. Typhimurium bacteria. This study used a factorial completely randomized design consisting of two factors, namely the concentration of the extract and the age of the inoculum with 4 repetitions. The concentration of the extract used was 5%, 10%, 20%, 40%, and 80%, equipped with the positive control (chloramphenicol), and negative control (aqua dest), while the age of the inoculum was based on the growth phase of the test bacteria, namely 3 hours, 6 hours, 18 hours. hours, and 24 hours. Antibacterial activity testing was carried out using the Disc Diffusion method (Kirby-Bauer Test). The test concentrations of 5%, 10%, 20%, 40%, and 80% test concentrations resulted in inhibition zone diameters of 3.41 mm, 5.4 mm, 6.04 mm, 7.43 mm, and 9.48 mm, respectively. The 80% concentration of ethanol extract from the kukukan leaf was effective in inhibiting the growth of S. typhimurium bacteria with an antibacterial effectiveness value of 60.81%.
Characterization And Identification of Halophilic Bacteria (Rastrelliger sp.) Extracellular Lipase Generator in Beringharjo Market Octavia, Bernadetta; Rakhmawati, Anna
Indonesian Journal of Bioscience (IJOBI) Vol. 2 No. 2 (2024): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v2i2.1056

Abstract

Advances in biotechnology have allowed for the widespread use of lipase enzymes. One of the producers of lipase enzymes is halophilic bacteria. This study aims to determine the characteristics and genus, as well as the kinship relationship between lipase-producing halophilic bacterial isolates from salted fish (Rastrelliger sp.) in Beringharjo Market, Yogyakarta. Salted fish samples were isolated on Nutrient Agar + 3% NaCl + Tween 80 + 0.1% Rhodamine-B selective media to grow lipase-producing halophilic bacteria. Identification was carried out by the matching profile method based on Bergey's Manual of Determinative Bacteriology. The kinship relationship between bacterial isolates was classified using MVSP 3.1 software with the UPGMA clustering algorithm and constructed in the form of a dendogram.
Exploration and Identification of Lichen in the Temon, Depok, and Tegalrejo Hafizh Adyatma; Anna Rakhmawati; Bernadetta Octavia
Indonesian Journal of Bioscience (IJOBI) Vol. 3 No. 2 (2025): Indonesian Journal of Bioscience (IJOBI)
Publisher : Department of Biology Education FMIPA Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v3i2.2484

Abstract

This research aims to identify the diversity of lichen species in three different areas: Temon, Kulon Progo; Depok, Sleman; and Tegalrejo, Magelang. Besides that to analyze their ecological role as environmental bioindicators. The method used was direct field observation with visual documentation using a mobile phone camera, followed by morphological identification. The results of the study successfully identified nine lichen species: Flavoparmelia caperata, Physcia atrostriata, Dirinaria applanata, Phlyctis argena, Acarospora socialis, Ropalospora viridis, Pyxine sorediata, Cryptothecia striata, and Graphis sp.. These species exhibited unique adaptations to various substrates (tree bark, rocks, and urban environments) and showed sensitivity to environmental changes, particularly air pollution. The mutualistic symbiosis between fungi and photobionts (algae/cyanobacteria) is key to the resilience of lichens in marginal habitats. These findings strengthen the potential of lichens as natural bioindicators and ecological pioneers, while also highlighting the need for further research to map the distribution and applications of lichens in Indonesia.
Co-Authors Abd. Rasyid Syamsuri Agus Taftazani Agus Taftazani Agus Taftazani Alphrazy, Rivaldo Anggraeni, Neli Dwi Septi Anna Rakhmawati Aris Bastianudin Astuti Astuti, Nadya Welli Aulia, Fera Ayuningtyas, Dwi Endang Gati Lestari Fellary Pangesti H Yulipriyanto, H Hafizh Adyatma Hartanti, Oktavia Dwi Heru Nurcahyo Imam Riadi Imtiyaz, Arifah Nuha Ixora Sartika Mercuriani Kun Sri Budiasih L Sembiring Madhani, Anisa Tiara Mohamad Habibi Nafisah, Aulia Erta Nahar Cahyandaru Nahar Cahyandaru Neli Dwi Septi Anggraeni Nirwana, Putri Nuri Nofia, Fifi Nugraha, Aditya Dirgantara Nur Rizki Putri Ramadhanti Nuradiah, Mawaddah Fatin Pertiwi, Melania Citra Pramesti, Ariska Hanum Pratama, Anggi Tias Putri Nur Indahsari Raphasya, Fhadinda Hizza Rina Rahmawati, Rina Rizka Apriani Putri Saladina, Salma Putri Saputra, Retno Sandi Sari, Herninda Dhama Sitanggang, Sarah Rapma Kristina Siti Nur Azizah Siti Umniyatie Sudarsono Suhandoyo Suhartini Suhartini Suhartini Suhartini Suhartini Tien Aminatun Triwibowo Yuwono