Endah Retno Palupi
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia

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STRUKTUR DAN METODE PERKECAMBAHAN BENIH ROTAN JERNANG (Daemonorops dransfieldii Rustiami) Eny Widajati; Nelly Fridayanti; Endah Retno Palupi; Satriyas Ilyas; Sri Wilarso Budi
Jurnal Perbenihan Tanaman Hutan Vol 10, No 1 (2022): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/bptpth.2022.10.1.81-99

Abstract

Buah rotan jernang merupakan hasil hutan bukan kayu bernilai ekonomi tinggi karena pada eksokarp buah terdapat resin. Buah rotan jernang dipanen sebelum benih mencapai masak fisiologis untuk mendapatkan resin kualitas tinggi sehingga tanaman tidak dapat meregenerasi secara alamiah dan rotan jernang di habitat alami semakin berkurang. Kendala dalam ketersedian benih bermutu adalah perkecambahan lama dan pertumbuhan bibit tidak seragam. Tujuan penelitian adalah untuk mendapatkan metode perkecambahan yang lebih cepat dan seragam, mempelajari proses perkecambahan, dan mengembangkan metode uji daya berkecambah. Percobaan pertama menggunakan RAL dua faktor. Faktor pertama adalah pencongkelan operkulum, terdiri atas dua taraf yaitu benih utuh dan benih tanpa operkulum. Faktor kedua adalah perendaman benih terdiri atas tiga taraf yaitu kontrol, perendaman menggunakan aquades dan KNO3 0,2%. Percobaan kedua menggunakan RAL satu faktor yaitu media perkecambahan terdiri atas media pasir dan cocopeat. Data dianalisis menggunakan SAS 9.4 dan diuji lanjut menggunakan DMRT pada taraf kepercayaan 5%. Pencongkelan operkulum dan tanpa perendaman dapat meningkatkan indeks vigor dan kecepatan tumbuh. Proses perkecambahan benih rotan jernang (benih tanpa operkulum) terdiri atas empat tahap yaitu terbentuk tangkai kotiledon, kotiledon ligule, akar dan daun. Hitungan pertama dan hitungan terakhir uji daya berkecambah adalah 72 dan 104 hari setelah tanam. Kriteria kecambah normal adalah panjang plumula minimum ±15 mm, akar berkembang dengan sempurna yaitu terdapat akar primer dan akar sekunder.  Media terbaik untuk perkecambahan benih rotan jernang adalah pasir. 
Optimasi Produksi dan Mutu Benih Padi Varietas PBM UBB 1 dengan Bakteri Pelarut Fosfat dan Pupuk Fosfat: Optimasi Produksi dan Mutu Benih Padi Varietas PBM UBB 1 dengan Bakteri Pelarut Fosfat dan Pupuk Fosfat Kartika Kartika; M. Rahmad Suhartanto; Abdul Munif; Endah Retno Palupi; Satriyas Ilyas
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 6 No 2 (2022): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.792 KB) | DOI: 10.33019/agrosainstek.v6i2.402

Abstract

One of the problems for phosphate fertilizing in ultisol soil is Al and Fe bounding and could be overcome by phosphate solubilizing bacteria activity.This study aimed to obtain the best dose of phosphate fertilizer with phosphate solubilizing bacteria for the production of upland rice seeds of the UBB1 PBM variety (Bangka Belitung University red rice 1) and its effect on seed quality. The research was carried out at the Research and Experimental Gardens – Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung, from August to December 2020. The design used was a split plot design, the main plot was phosphate solubilizing bacteria (treatment and control), and the subplots were fertilizers. phosphate (P1– quarter dose, P2– half dose, P2–three-quarter dose, P4– full dose). Seeds produced in the field were tested in the laboratory for viability and vigor. In the field, phosphate solubilizing bacteria (Burkholderia sp) can replace half the dose of phosphate fertilizer. This was indicated by the seed weight of plant-1 which did not differ between full dose + control and half dose + treatment. This fact was also identified in the viability and vigor of the seeds in laboratory testing. There was no significant difference in germination rate and vigor index between the two treatments. This finding shows that phosphate solubilizing bacteria can be applied for efficiency of phosphate fertilization and guaranteeing seed production and seed quality
Karakter Agro-morfologi dan Periode After-ripening Benih Padi Lanras Lokal Potensial asal Timor-Leste Luis Manuel Branco; Satriyas Ilyas; Bambang Sapta Purwoko; Agus Purwito; Endah Retno Palupi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.498 KB) | DOI: 10.24831/jai.v50i3.43102

Abstract

Benih padi yang baru dipanen umumnya tidak berkecambah walaupun dikecambahkan pada kondisi optimum yang disebut after-ripening, dan secara alamiah akan hilang setelah waktu tertentu. Penelitian ini bertujuan melakukan karakterisasi agro-morfologi dan menetapkan periode after-ripening benih 5 lanras potensial di Timor-Leste. Penelitian dilaksanakan pada bulan April 2018-November 2019. Penanaman dan pengamatan karakter agro-morfologi 5 lanras potensial Timor-Leste, yaitu Hare R-oitu, Fos Mean, Hare Belit, Hare Nona Portu, dan Ale Mamea Ula Lesa, dilaksanakan di Desa Caibada Distrik Baucau, Timor-Leste. Pengamatan periode after ripening dan mutu benih ke lima lanras tersebut dilaksanakan di Laboratorium Pengujian dan Penyimpanan Benih, Departemen Agronomi dan Hortikultura, IPB University, dan dirancang dalam rancangan acak lengkap (RAL) dua faktor dengan empat ulangan. Faktor pertama adalah 5 lanras tersebut di atas dan faktor kedua adalah periode simpan yang terdiri atas 2, 3, 4, 5, 6, 7, dan 8 minggu setelah panen (MSP). Hasil penelitian menunjukkan bahwa 5 lanras potensial di Tinmor-Leste mempunyai keragaan tanaman yang pendek (76.3-105.50 cm), tergolong berumur sedang (129-141 HSS), dan jumlah anakan produktif tinggi (17.5-53.50 anakan), kecuali Ale Mamea Ula Lesa. Lima lanras tersebut mempunyai potensi produktivitas yang tinggi berkisar 8.1-9.8 ton ha-1. Periode after-ripening lanras Hare Belit dan Hare Nona Portu berakhir pada 2 MSP, Hare R-oitu pada 6 MSP, sedangkan Ale Mamea Ula Lesa dan Fos Mean pada 8 MSP. Semakin lama benih disimpan rasio kandungan ABA/GA3 semakin menurun dari 0.53-1.22 ppm pada 2 MSP menjadi 0.45-0.93 ppm pada 4 MSP. Lanras Hare Belit dan Hare Nona Portu merupakan lanras yang potensial dikembangkan menjadi varietas nasional di Timor-Leste, masing-masing untuk pengembangan di dataran rendah dan dataran sedang. Kata kunci: karakterisasi, intensitas dormansi, persentase perkecambahan, mutu benih
Fine Bubbles Utilization to Increase Germination Rate of Oil Palm (Elaeis guineensis L. Jacq) Seeds Mohamad Arif; Satriyas Ilyas; Eny Widajati; Abdul Qadir; Edy Suprianto; Endah Retno Palupi
AGRIVITA, Journal of Agricultural Science Vol 45, No 1 (2023)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v45i1.3908

Abstract

The current method to break oil palm seed dormancy is the dry heat method which requires approximately 90 days for seeds to germinate with an average germination percentage. Therefore, hastening the process with a higher ratio of germinated seeds becomes necessary. This experiment aims to hasten and increase germination percentage by applying fine bubble water during the dormancy-breaking process. A seed lot derived from one bunch as replication is divided into two sets where fiber plugs from one of the sets are removed while the other set remains intact. Each set was re-divided into six experimental units soaked with tap water or fine bubble water for 1 to 3 days. The result showed that applying fine bubble water provided a better germination rate than tap water treatments. However, the total germination percentage between all treatments does not differ significantly. The germination peak of oil palm seed in the fine bubble water treatments occurred on the 26th day, 8 days faster than in the tap water treatments. The occurrence showed the potential use of fine bubble water application during dormancy breaking using the dry heat method.
Flowering and Seed Development Characteristic of Citrus Derived from Somatic Hybridization of Mandarin Satsuma (Citrus unshiu Marc.) and Siam Madu (Citrus nobilis Lour.) Imroatus Sa’adah; Eny Widajati; Mia Kosmiatin; Endah Retno Palupi
AGRIVITA, Journal of Agricultural Science Vol 44, No 1 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i1.2978

Abstract

The somatic hybridization between Mandarin Satsuma (Citrus unshiu Marc.) and Siam Madu (Citrus nobilis Lour.) is expected to produce progenies having sweet seedless fruit. The research was aimed to study flowering biology, fruit and seed development to identify parthenocarpic lines derived from somatic hybridization. The research was carried out at Pacet Experimental Station of ICABIOGRAD, Cianjur, West Java (1150 m asl), during August 2019-July 2020. The research materials were 15 citrus lines derived from somatic hybridization between Mandarin Satsuma and Siam Madu, of which 5 plants (± 2 years old) per line were prepared. Observation was carried out on flowering phenology, flower morphology, fruit development, pollen viability, and stigma receptivity. Seedless fruits from un-pollinated, selfpollinated, and cross-pollinated flowers of each line were investigated. The results showed that flower morphology of the 15 citrus lines varied in the number of petals, flower diameter, pistil length relative to the stamen, and number of stamen. The development from bud emergence to fruit ripening also varied among and within lines, ranging from 212 to 316 days. Among the lines, FS 25 showed a stronger character towards stimulative parthenocarpy. FS 84 and FS 89 were potential sources of pollen (pollenizer) to produce seedless fruits.
Invigoration Increased Quality and Storability of True Seed of Shallot (Allium ascalonicum L.) Qudus Sabha Adhinugraha; Eny Widajati; Endah Retno Palupi
Journal of Tropical Crop Science Vol. 9 No. 02 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.02.145-155

Abstract

Seed invigoration is a pre-planting seed treatment by balancing the seed water potential and stimulating their metabolic activities so that the seeds germinate simultaneously. In this study, invigoration treatments were applied to improve the quality of the true seeds of shallots (TSS) that had started to deteriorate. Invigoration increases the moisture content of the seeds so the seeds need to be re-dried to extend their storability. The aims of the study were to determine the best invigoration treatment and the drying method to maintain the benefit of invigoration, and to prolong TSS storability after invigoration and drying. This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia, and consisted of three experiments, i.e., invigoration of deteriorating TSS to improve their viability and vigor, drying after invigoration to extend seed storability, and seed storability after invigoration and drying treatments. The first experiment was arranged in completely randomized design with one-factor (invigoration solutions), i.e. control, medium containing 3% KNO3, 50 ppm GA3, 0.5 % ZnSO4 or PEG6000 -10 bar. The second experiment was conducted using a two-factor completely randomized design, drying temperature (20˚C, 30˚C, and 40˚C), and drying duration (8 hours, 16 hours, 32 hours, and 48 hours). The third experiment was arranged in a nested design with two factors. The first factor was a seed storage condition, i.e., ambient temperature, air-conditioned room, and refrigerator. The second factor was seed packages, i.e., aluminum foil and polypropylene plastic. Non-invigorated seeds were used as control. The studies were conducted on two shallot varieties separately, “Trisula” and “Lokananta”, except in the third experiment which only used “Lokananta”. The results showed that invigoration in 3 % KNO3 and 50 ppm GA3 effectively improved the vigor of deteriorating TSS. Drying the seeds at 40˚C for 8 hours was the most effective method to reduce seed moisture content without reducing the benefit of invigoration. Storing the invigorated TSS in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum foil or plastic packaging until 14 weeks maintained the benefit of invigoration. The quality of the invigorated TSS was maintained until 14 weeks in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum
Ultrafine Bubbles Water priming to improve viability and vigor of bean (Phaseolus vulgaris) seeds Yuni Nurfiana; M. Rahmad Suhartanto; Endah Retno Palupi; Y. Aris Purwanto
Journal of Tropical Crop Science Vol. 10 No. 01 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.1.8-16

Abstract

Priming is a physiological technique of hydration of seed to improve metabolic processes before germination to accelerate germination and seedling growth under normal and stress conditions. This research is aimed to study the pretreatment using ultra-fine bubble (UFB) water to increase viability and vigor of seeds before planting (pre-planting) and during storage (pre-storage). This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia. The research consisted of two experiments, i.e., UFB water priming to increase viability and vigor of bean seeds and its storability after priming. The first experiment was arranged using a completely randomized design with a combination of seed lot treatments (L1: initial germination percentage (GP) about 80%, L2: initial GP around 70%, and L3: initial GP around 60%) with priming (P0: no priming/control, P1: soaked in distilled water for 60 minutes, P2: distilled water for 120 minutes, P3: 8 ppm UFB water for 60 minutes, P4: 8 ppm UFB water for 120 minutes, P5: 20 ppm UFB water for 60 minutes, P6: UFB water 20 ppm for 120 minutes). The second experiment was arranged using a nested design with the main factor being the condition of the storage room and priming as the second factor nested in the main factor. The storage room condition factors consisted of KM: room conditions (temperature 28±5˚C, RH 73±7%) and AC: air-conditioned room (18±2 ̊C, RH 61±7%). The priming factor consisted of two levels, namely P0: without priming and P1: priming with 20 ppm UFB water soaked for 120 minutes. The results showed that priming treatment with UFB water 20 ppm for 120 minutes as a pre-planting treatment can increase the viability of bean seeds, particularly for seeds with low initial viability, and primed bean seeds with an initial viability of approximately 80% were able to retain their viability for 16 weeks of storage in an air-conditioned room.
Quality and Production Potentials of Various Types of Taro Cormel Rafi Fauzan; Eny Widajati; Endah Retno Palupi; Edi Santosa
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.137-146

Abstract

Taro is a promising food crop that can be developed as a significant source of carbohydrates; however, a major obstacle is the unavailability of high-quality planting materials. Due to the difficulty in obtaining seeds, taro cultivation generally relies on vegetative propagation materials such as cormels, cormlets, and stolons. The advantage of using vegetative propagation material is that it retains the same characteristics as the parent plant. This research aimed to evaluate the quality of various types of cormels as taro planting material, study the potential of different types of cormels on the growth and production of taro plants, and investigate the growth, development, and time required to produce cormels from various types of cormels and their potential in cormel seed production. The research consisted of two experiments: the first experiment evaluated the quality of several types of cormels from two taro accessions (S24 and S28) using a completely randomized design with two factors (accession and types of cormels—primary, secondary, and tertiary), while the second experiment studied the growth and development of cormels from various types of cormels. The best quality seeds were obtained from primary cormels directly attached to the main tubers, with the germination rate highest for primary cormels at 53.25%, followed by secondary cormels at 51.59%, and tertiary cormels at 39.42%. Accession S24 showed a faster emergence rate (30.26 days) compared to S28 (58.08 days), and in S24, there was no significant difference between types of cormels for this parameter. In contrast, in S28, primary cormels had a significant emergence advantage (33.65 days) over secondary and tertiary cormels (62.57 and 78.02 days, respectively). Additionally, primary cormels were formed 8 weeks after planting, while secondary and tertiary cormels appeared 12 weeks after planting. These findings suggest that primary cormels should be prioritized for use in taro propagation to improve growth, development, and yield, highlighting their potential in cormel seed production and offering a viable solution to the challenge of seed availability in taro cultivation.
Isolation and Characterization of Phosphate Solubilizing Bacteria from Upland Rice Cultivation Areas in Bangka Regency Kartika, Kartika; Munif, Abdul; Palupi, Endah Retno; Ilyas, Satriyas; Suhartanto, Muhamad Rahmad
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84500

Abstract

The availability of phosphorus (P) in ultisol acid soils presents a significant challenge due to its attachment to aluminum (Al) or iron (Fe) compounds. A potential solution to address this issue is the utilization of phosphate solubilizing bacteria (PSB). Therefore, this study aimed to analyze the potential of PSB originating from upland rice cultivation on ultisol soils. The bacterial isolates were obtained from soil samples taken from the rhizosphere area and root tissue of upland rice plants cultivated in Payabenua and Saing Villages, Bangka Regency. The pathogenicity testing encompassed hypersensitivity and hemolysis tests, while the P solubilization included the evaluation of the phosphate solubilizing index (PSI) and P dissolution. Subsequently, the selected isolates were subjected to phosphatase enzyme and organic acid content assessment. The results showed a total of 120 isolates, predominantly distributed in the Payabenua area and primarily consisting of endophytic bacteria. Among the six selected isolates, genus Burkholderia dominated four isolates, while the remaining isolates belonged to genus Serratia. Furthermore, in Burkholderia vietnamiensis, the solubility value of P in AlPO4 and Ca3(PO4)2 liquid media exhibited a range of 0.0013 to 0.0344% and 0.0008 to 0.1842%, respectively. 
Pengaruh Suhu Ruang Simpan dan Perlakuan Pasca Penyimpanan terhadap Mutu dan Produktivitas Umbi Benih Bawang Merah (Allium cepa L. group Aggregatum) Alvita Sekar Sarjani; Endah Retno Palupi; Muhammad Rahmad Suhartanto; Y. Aris Purwanto
Jurnal Hortikultura Indonesia (JHI) Vol 9 No 2 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340 KB) | DOI: 10.29244/jhi.9.2.111-121

Abstract

ABSTRACTThe fluctuative price of shallot in Indonesia is mainly due to discontinuous supply. Shallot is usually planted three times a year. Lack of supply occurs during July to October. Therefore, the produce need to be stored to ensure its availability during off season, not only for consumption but also as seed bulb for the following planting season. The research was aimed to maintain the quality of seed bulbs during twelve weeks storage and to evaluate productivity of the seed bulbs after storage. Shallot seed bulbs of Bima Brebes was used for the research that was devided into two steps. The first step was arranged in nested design, in which seed bulb was stored at 0 0C, 5 0C, 10 0C and ambient temperature nested into storage period i.e 0, 3, 6, 9 and 12 weeks with four replications. The second step was evaluation of productivity of the seed bulbs that was arranged in nested design. The seed bulbs, after being stored at diferent condition, was subjected to different acclimatization treatments i.e. gradual increase of temperatures for 3 days and direct change to ambient temperature for one day, to devernalize the seed bulbs and replicated our times. The results showed that the dormant period of shallot seed bulbs lasted for 8 weeks after harvest (6 week after storage) as indicated by germination and vigor index of >90%. The termination of dormancy coincided with a rise in GA, IAA and cytokines as well as ABA. Storing the seed bulbs for 3 months in 5 0C could maintain its viability and vigor >90%, with 9.8% of total damage and 15.6% of weight loss. The seed bulbs grew normally and produced 30.2 g of bulb per plant. The percentage of flowering plant of gradually acclimatized seed bulbs previously stored at 5 0C (10.3%) was not significantly different from those directly acclimatized at ambient temperature (12%).Keywords: ABA, cytokinin, dormancy, GA, weight lossABSTRAKPenyebab utama fluktuasi harga bawang merah di pasar adalah ketersediaan umbi bawang merah yang tidak stabil. Di daerah sentra produksi, bawang merah ditanam tiga kali dalam setahun. Bulan Juli sampai Oktober adalah periode hasil panen rendah. Penyimpanan umbi merupakan salah satu upaya untuk menjamin ketersediaannya di luar musim panen, tidak hanya untuk konsumsi tetapi juga memastikan ketersediaan umbi sebagai benih pada musim tanam selanjutnya. Penelitian ini bertujuan mempertahankan kualitas benih umbi selama 12 minggu disimpan dan mengevaluasi produktivitasnya setelah penyimpanan. Bahan yang digunakan adalah benih umbi bawang merah varietas Bima Brebes. Penelitian dibagi dalam dua tahap. Tahap pertama adalah penyimpanan benih umbi dirancang dalam nested design yang mana benih umbi disimpan pada suhu 0 0C, 5 0C, 10 0C dan suhu ruang tersarang pada waktu penyimpanan yang terdiri atas 0, 3, 6, 9 dan 12 minggu dan diulang empat kali. Tahap kedua adalah evaluasi produktivitas benih umbi setelah disimpan dirancang dalam nested design. Umbi yang telah disimpan (12 minggu) pada masing-masing kondisi simpan diberi perlakuan aklimatisasi, yaitu suhu berjenjang (3 hari) dan suhu ruang langsung (1 hari), untuk mencegah pembungaan. Setiap perlakuan diulang sebanyak empat kali (ulangan tersarang pada aklimatisasi). Hasil penelitian menunjukkan bahwa benih umbi mengalami dormansi sampai 8 minggu setelah panen (6 minggu setelah simpan), ditandai dengan daya berkecambah dan indeks vigor di atas 90%. Berakhirnya dormansi benih umbi bersamaan dengan peningkatan kandungan giberelin, auksin, dan sitokinin mengimbangi peningkatan asam absisat. Penyimpanan benih selama 12 minggu pada suhu 5 0C dapat mempertahankan viabilitas dan vigor di atas 90% dengan kerusakan (umbi bertunas, chilling injury, hampa atau busuk) sebesar 9.8% dan susut bobot sebesar 15.6%. Setelah disimpan selama 12 minggu benih dapat tumbuh normal dan memproduksi 30.2 g umbi per tanaman. Aklimatisasi suhu berjenjang umbi benih yang telah disimpan pada suhu 5 0C menghasilkan persentase pembungaan (10.3%) yang tidak berbeda nyata dengan aklimatisasi suhu ruang langsung (12%).Kata kunci: ABA, dormansi, GA, sitokinin, susut bobot
Co-Authors , Krisantini , Misnen , Yudiwanti ABDUL MUNIF Abdul Munif Abdul Qadir Agus Purwito Ajisyahputra, Nikko Rizky Alvita Sekar Sarjani ANAS D SUSILA Anas D. Susila Andry Indrawan Aris Hairmansis Bambang Sapta Purwoko Chintya Dwi Septianingrum Christian Simanjuntak Christian Simanjuntak dan Faiza Chairani Suwarno dan Nurita Toruan Mathius Dian Fahrianty Dian Hapsari Ekaputri Dida Syamsuwida Dudin Supti Wahyudin Edi Santosa Edy Suprianto Ekowati Nursiam Harliani ENDANG MURNIATI Endang Murniati Eny Widajati Erianna Ayu Emkha Putri Esty Puri UTAMI Fatiani Manik Gani Jawak Harahap, Ade Tika Sari Husna Fatima Eprilian Imroatus Sa’adah Iskandar Lubis ISKANDAR ZULKARNAEN SIREGAR Joko Mulyono Kartika Kartika Kartika Kartika Karyadi Wanafiah Kusumastuti, Hamiddah Intan Leli Kurniasari Leli Kurniasari Luis Manuel Branco MATANA, YULIANUS R. Mega Rahayu MELATI MELATI Memen Surachman Mia Kosmiatin Mira Landep Widiastuti Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Muhamad Syukur MURNIATI, ENDANG Nelly Fridayanti Ni Made Armini Wiendi Nurita TORUAN-MATHIUS Nurul Afifah Nutrita Toruan Mathius Purwono Purwono Qadir, Abdul Qudus Sabha Adhinugraha Rafi Fauzan Rini Rosliani Rini Rosliani Rini Rosliani Rini Rosliani Riski Meliya Ningsih Roedhy Poerwanto Rotua Melisa Sidabutar Rotua Melisa Sidabutar Saipulloh , Satriyas Ilyas Siti Fadhilah, Siti Sri Suhesti Sri Wilarso Budi Suhartanto, Muhammad Rahmad Syamsuddin Syamsuddin Winarso D. Widodo Y. Aris Purwanto Yopy Dedywiryanto YULIANUS R. MATANA Yuni Nurfiana Yusdar Hilman Yusdar Hilman Yusdar Hilman Zelda, Fauzulin Kumala