Endah Retno Palupi
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia

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Invigoration Increased Quality and Storability of True Seed of Shallot (Allium ascalonicum L.) Adhinugraha, Qudus Sabha; Widajati, Eny; Palupi, Endah Retno
Journal of Tropical Crop Science Vol. 9 No. 02 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.02.145-155

Abstract

Seed invigoration is a pre-planting seed treatment by balancing the seed water potential and stimulating their metabolic activities so that the seeds germinate simultaneously. In this study, invigoration treatments were applied to improve the quality of the true seeds of shallots (TSS) that had started to deteriorate. Invigoration increases the moisture content of the seeds so the seeds need to be re-dried to extend their storability. The aims of the study were to determine the best invigoration treatment and the drying method to maintain the benefit of invigoration, and to prolong TSS storability after invigoration and drying. This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia, and consisted of three experiments, i.e., invigoration of deteriorating TSS to improve their viability and vigor, drying after invigoration to extend seed storability, and seed storability after invigoration and drying treatments. The first experiment was arranged in completely randomized design with one-factor (invigoration solutions), i.e. control, medium containing 3% KNO3, 50 ppm GA3, 0.5 % ZnSO4 or PEG6000 -10 bar. The second experiment was conducted using a two-factor completely randomized design, drying temperature (20˚C, 30˚C, and 40˚C), and drying duration (8 hours, 16 hours, 32 hours, and 48 hours). The third experiment was arranged in a nested design with two factors. The first factor was a seed storage condition, i.e., ambient temperature, air-conditioned room, and refrigerator. The second factor was seed packages, i.e., aluminum foil and polypropylene plastic. Non-invigorated seeds were used as control. The studies were conducted on two shallot varieties separately, “Trisula” and “Lokananta”, except in the third experiment which only used “Lokananta”. The results showed that invigoration in 3 % KNO3 and 50 ppm GA3 effectively improved the vigor of deteriorating TSS. Drying the seeds at 40˚C for 8 hours was the most effective method to reduce seed moisture content without reducing the benefit of invigoration. Storing the invigorated TSS in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum foil or plastic packaging until 14 weeks maintained the benefit of invigoration. The quality of the invigorated TSS was maintained until 14 weeks in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum
Ultrafine Bubbles Water priming to improve viability and vigor of bean (Phaseolus vulgaris) seeds Nurfiana, Yuni; Suhartanto, M. Rahmad; Palupi, Endah Retno; Purwanto, Y. Aris
Journal of Tropical Crop Science Vol. 10 No. 01 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.1.8-16

Abstract

Priming is a physiological technique of hydration of seed to improve metabolic processes before germination to accelerate germination and seedling growth under normal and stress conditions. This research is aimed to study the pretreatment using ultra-fine bubble (UFB) water to increase viability and vigor of seeds before planting (pre-planting) and during storage (pre-storage). This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia. The research consisted of two experiments, i.e., UFB water priming to increase viability and vigor of bean seeds and its storability after priming. The first experiment was arranged using a completely randomized design with a combination of seed lot treatments (L1: initial germination percentage (GP) about 80%, L2: initial GP around 70%, and L3: initial GP around 60%) with priming (P0: no priming/control, P1: soaked in distilled water for 60 minutes, P2: distilled water for 120 minutes, P3: 8 ppm UFB water for 60 minutes, P4: 8 ppm UFB water for 120 minutes, P5: 20 ppm UFB water for 60 minutes, P6: UFB water 20 ppm for 120 minutes). The second experiment was arranged using a nested design with the main factor being the condition of the storage room and priming as the second factor nested in the main factor. The storage room condition factors consisted of KM: room conditions (temperature 28±5˚C, RH 73±7%) and AC: air-conditioned room (18±2 ̊C, RH 61±7%). The priming factor consisted of two levels, namely P0: without priming and P1: priming with 20 ppm UFB water soaked for 120 minutes. The results showed that priming treatment with UFB water 20 ppm for 120 minutes as a pre-planting treatment can increase the viability of bean seeds, particularly for seeds with low initial viability, and primed bean seeds with an initial viability of approximately 80% were able to retain their viability for 16 weeks of storage in an air-conditioned room.
Quality and Production Potentials of Various Types of Taro Cormel Fauzan, Rafi; Widajati, Eny; Palupi, Endah Retno; Santosa, Edi
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.137-146

Abstract

Taro is a promising food crop that can be developed as a significant source of carbohydrates; however, a major obstacle is the unavailability of high-quality planting materials. Due to the difficulty in obtaining seeds, taro cultivation generally relies on vegetative propagation materials such as cormels, cormlets, and stolons. The advantage of using vegetative propagation material is that it retains the same characteristics as the parent plant. This research aimed to evaluate the quality of various types of cormels as taro planting material, study the potential of different types of cormels on the growth and production of taro plants, and investigate the growth, development, and time required to produce cormels from various types of cormels and their potential in cormel seed production. The research consisted of two experiments: the first experiment evaluated the quality of several types of cormels from two taro accessions (S24 and S28) using a completely randomized design with two factors (accession and types of cormels—primary, secondary, and tertiary), while the second experiment studied the growth and development of cormels from various types of cormels. The best quality seeds were obtained from primary cormels directly attached to the main tubers, with the germination rate highest for primary cormels at 53.25%, followed by secondary cormels at 51.59%, and tertiary cormels at 39.42%. Accession S24 showed a faster emergence rate (30.26 days) compared to S28 (58.08 days), and in S24, there was no significant difference between types of cormels for this parameter. In contrast, in S28, primary cormels had a significant emergence advantage (33.65 days) over secondary and tertiary cormels (62.57 and 78.02 days, respectively). Additionally, primary cormels were formed 8 weeks after planting, while secondary and tertiary cormels appeared 12 weeks after planting. These findings suggest that primary cormels should be prioritized for use in taro propagation to improve growth, development, and yield, highlighting their potential in cormel seed production and offering a viable solution to the challenge of seed availability in taro cultivation.
Kajian Karakter Ketahanan terhadap Cekaman Kekeringan pada Beberapa Genotipe Bibit Kelapa Sawit (Elaeis guineensis Jacq.) Palupi, Endah Retno; Dedywiryanto, Yopy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.979 KB) | DOI: 10.24831/jai.v36i1.1341

Abstract

The research was aimed to study the response of oil palm seedlings to drought stress and to determine characters which would be useful in selection for drought resistance. This research was conducted at PT Dami Mas Sejahtera field station and SMART Research Institute laboratory Libo, Riau from April to August 2005. The research was arranged in split plot design with two factors in randomized block design. The main plot was soil water content, i.e. 100%FC/field capacity : 24+1%; 75%FC : 18+1%; 50%FC : 12+1%; and 25%FC : 6+1%, whereas the subplot was four genotypes: G1: 635xAP.01, G2: 635x742.316, G3:15x742.316 and G4: 15xAP.01. The result showed that only soil water content as low as 25%FC could significantly inhibit seedling growth, as indicated by reduction of leave water content,  shoot and root dry weight, seedling height, root length and volume; and an increase of water deficit. Soil water content ranging from 50-100% FC did not give any significant effect. Based on the physiological responds, G1 and G3 were relatively more resistant to drought stress than G2 and G4. Leaf water content was the easiest, cheapest, fastest and non-destructive variable to be used for early selection of drought resistant oil palm seedlings.   Key words:  Oil palm, seedling, character for selection, drought resistant
Efektivitas Bahan Pelapis Benih terhadap Penyerapan Fosfat dan Pertumbuhan Bibit Kelapa Sawit ,, Saipulloh; Palupi, Endah Retno; Widajati, Eny; Mathius, dan Nurita Toruan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.151 KB) | DOI: 10.24831/jai.v45i1.12236

Abstract

One way of overcome phosphate deficiency in oil palm plantation is seed enrichment using phosphate solvent microbes. This technique is expected to accelerate phosphate availability for plant. The aim of the research was to identify the best coating materials for seed enrichment that compatible with Burkholderia sp. in order to increase phosphate absorption, seed storability, and growth of oil palm seedlings. This research consisted of two consecutive experiments. The first experiment was arranged in randomized block design, with nine treatments of different seed coating materials. The second experiment was arranged in nested design, with main factor of 11 different seed coating materials and nested factors were five storage periods. The data was collected on growth parameters of seedling height, root length, trunk height, dry weight; phosphate absorption, and DGGE analysis. The research showed there were three best seed coating materials of CMC 1.5%, CMC 2% + gypsum 1.5%, and CMC 1.5% + talc 1%. The coating material enhances seedling growth and phosphate absorption. Seed coating and enrichment maintained seedling vigor for three days in the storage. Keywords: Burkholderia sp., carboxyl methyl cellulose, phosphate solvent microbial, seed enrichment, seed storage
Penyimpanan Serbuk Sari Jagung dan Potensinya untuk Produksi Benih Hibrida Fariroh, Indri; Palupi, Endah Retno; Suwarno, dan Faiza Chairani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 2 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.285 KB) | DOI: 10.24831/jai.v45i2.12535

Abstract

Production of maize hybrid seed is hindered by relatively poor production by the male line pollen; short live of the pollen which was ascribed to desiccation and dehydration sensitive and flowering asynchrony between male and female lines. The aims of this study were to enhance production and viability of male line pollen and also to determine the suitable storage condition for maintaining pollen viability in order to be used for production of hybrid seed. The research consisted of two experiments. The first experiment was arranged in split plot randomized block design with NPK dosages as main plot (0, 300, 600, 900 kg ha-1) and application of boron as sub-plot (0, 1.5, 3, 4.5, 6 kg ha-1), replicated three times. The second experiment used randomized block design with two factors, i.e. storage temperature (-196 oC and -20 oC) and storage period (0, 1, 2, 3, 4 weeks). The result showed that NPK 900 kg ha-1 increased the number of spike per tassel from 9.3 to 10.7. Application of NPK 600 kg ha-1 or boron 1.5 kg ha-1 improved pollen viability. Combination of NPK 900 kg ha-1 and boron 6 kg ha-1 resulted in the highest pollen viability of 95.3%. Pollen after being stored for four weeks either in -196 ºC or -20 ºC was possible to be used for seed production with 20% seed set.Keywords: boron, freezer, liquid nitrogen, NPK dosage, pollen viability  
Kualitas Benih Berdasarkan Warna Kulit dan Bagian Buah, serta Kualitas Bibit Srikaya dengan Pemberian PGPR dan CaCO3 Riski Meliya Ningsih; Widajati, Eny; Endah Retno Palupi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 2 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1036.146 KB) | DOI: 10.24831/jai.v49i2.36381

Abstract

Benih srikaya yang bermutu sangat diperlukan untuk mendukung pengembangan bibit tanaman srikaya. Penelitian ini bertujuan menentukan tingkat kemasakan dan posisi benih dalam buah yang tepat guna mendapatkan benih bermutu dan mendapatkan perlakuan terbaik untuk meningkatkan performa bibit melalui penggunaan rizobakteri dan CaCO3. Percobaan pertama menggunakan rancangan acak lengkap (RAL) dua faktor dengan faktor pertama adalah warna kulit buah dan faktor kedua adalah posisi benih dalam buah. Percobaan kedua menggunakan RAL dengan rizobakteri, CaCO3, dan kombinasi keduanya sebagai faktor tunggal. Waktu hitungan pertama pengamatan daya berkecambah pada 36 hari setelah tanam (HST) dan hitungan akhir pada 56 HST. Kualitas benih terbaik diperoleh dari warna kulit buah kuning berdasarkan bobot kering benih, daya berkecambah, indeks vigor, bobot kering kecambah normal, dan kecepatan tumbuh benih. Vigor tumbuh benih tertinggi diperoleh dari buah dengan warna kulit kuning dari bagian ujung dan pangkal buah. Perlakuan CaCO3 mempengaruhi pemanjangan akar, lebar daun, diameter batang, dan panjang daun. Aplikasi CaCO3 memberikan pengaruh terbaik terhadap pertumbuhan bibit diikuti dengan perlakuan Pseudomonas + Actimomycetes + CaCO3. Kata kunci: Annona squamosa, batang bawah, hitungan akhir, indeks vigor, rizobakteri
Karakter Agro-morfologi dan Periode After-ripening Benih Padi Lanras Lokal Potensial asal Timor-Leste Branco, Luis Manuel; Ilyas, Satriyas; Purwoko, Bambang Sapta; Agus Purwito; Palupi, Endah Retno
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.498 KB) | DOI: 10.24831/jai.v50i3.43102

Abstract

Benih padi yang baru dipanen umumnya tidak berkecambah walaupun dikecambahkan pada kondisi optimum yang disebut after-ripening, dan secara alamiah akan hilang setelah waktu tertentu. Penelitian ini bertujuan melakukan karakterisasi agro-morfologi dan menetapkan periode after-ripening benih 5 lanras potensial di Timor-Leste. Penelitian dilaksanakan pada bulan April 2018-November 2019. Penanaman dan pengamatan karakter agro-morfologi 5 lanras potensial Timor-Leste, yaitu Hare R-oitu, Fos Mean, Hare Belit, Hare Nona Portu, dan Ale Mamea Ula Lesa, dilaksanakan di Desa Caibada Distrik Baucau, Timor-Leste. Pengamatan periode after ripening dan mutu benih ke lima lanras tersebut dilaksanakan di Laboratorium Pengujian dan Penyimpanan Benih, Departemen Agronomi dan Hortikultura, IPB University, dan dirancang dalam rancangan acak lengkap (RAL) dua faktor dengan empat ulangan. Faktor pertama adalah 5 lanras tersebut di atas dan faktor kedua adalah periode simpan yang terdiri atas 2, 3, 4, 5, 6, 7, dan 8 minggu setelah panen (MSP). Hasil penelitian menunjukkan bahwa 5 lanras potensial di Tinmor-Leste mempunyai keragaan tanaman yang pendek (76.3-105.50 cm), tergolong berumur sedang (129-141 HSS), dan jumlah anakan produktif tinggi (17.5-53.50 anakan), kecuali Ale Mamea Ula Lesa. Lima lanras tersebut mempunyai potensi produktivitas yang tinggi berkisar 8.1-9.8 ton ha-1. Periode after-ripening lanras Hare Belit dan Hare Nona Portu berakhir pada 2 MSP, Hare R-oitu pada 6 MSP, sedangkan Ale Mamea Ula Lesa dan Fos Mean pada 8 MSP. Semakin lama benih disimpan rasio kandungan ABA/GA3 semakin menurun dari 0.53-1.22 ppm pada 2 MSP menjadi 0.45-0.93 ppm pada 4 MSP. Lanras Hare Belit dan Hare Nona Portu merupakan lanras yang potensial dikembangkan menjadi varietas nasional di Timor-Leste, masing-masing untuk pengembangan di dataran rendah dan dataran sedang. Kata kunci: karakterisasi, intensitas dormansi, persentase perkecambahan, mutu benih
Co-Authors , Krisantini , Misnen , Yudiwanti ,, Saipulloh ABDUL MUNIF Abdul Munif Abdul Qadir Agus Purwito Ajisyahputra, Nikko Rizky ANAS D SUSILA Anas D. Susila Andry Indrawan Aris Hairmansis Bambang Sapta Purwoko Branco, Luis Manuel Christian Simanjuntak Christian Simanjuntak Daniel Happy Putra Dian Fahrianty Dian Hapsari Ekaputri Dida Syamsuwida Dudin Supti Wahyudin Edi Santosa Edy Suprianto Ekowati Nursiam Harliani Endang Murniati ENDANG MURNIATI Eny Widajati Eprilian, Husna Fatima Esty Puri UTAMI Fatiani Manik Fauzan, Rafi Gani Jawak Harahap, Ade Tika Sari Imroatus Sa’adah Iskandar Lubis ISKANDAR ZULKARNAEN SIREGAR Joko Mulyono Kartika Kartika Kartika Kartika Karyadi Wanafiah Kusumastuti, Hamiddah Intan Leli Kurniasari Leli Kurniasari MATANA, YULIANUS R. Mathius, dan Nurita Toruan Mega Rahayu MELATI MELATI Memen Surachman Mia Kosmiatin Mira Landep Widiastuti Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Muhamad Syukur Muhammad Afif MURNIATI, ENDANG Nelly Fridayanti Ni Made Armini Wiendi Nurfiana, Yuni Nurita TORUAN-MATHIUS Nutrita Toruan Mathius Purwono Purwono Putri, Erianna Ayu Emkha Qadir, Abdul Qudus Sabha Adhinugraha Rini Rosliani Rini Rosliani Rini Rosliani Rini Rosliani Riski Meliya Ningsih Roedhy Poerwanto Rotua Melisa Sidabutar Rotua Melisa Sidabutar Sarjani, Alvita Sekar SATRIYAS ILYAS Satriyas Ilyas Septianingrum, Chintya Dwi Siti Fadhilah, Siti Sri Suhesti Sri Wilarso Budi Suhartanto, Muhammad Rahmad Suwarno, dan Faiza Chairani Syamsuddin Syamsuddin Winarso D. Widodo Yopy Dedywiryanto YULIANUS R. MATANA Yusdar Hilman Yusdar Hilman Yusdar Hilman Zelda, Fauzulin Kumala