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Journal : BALI MEDICAL JOURNAL (BMJ)

Relative Humidity of 40% Inhibiting the Increase of Pulse Rate, Body Temperature, and Blood Lactic Acid During Exercise Sandi, N.; Adiputra, N.; Pangkahila, A.; Adiatmika, P. G.
BALI MEDICAL JOURNAL Vol 5 No 2 (2016)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Excessive sweating of the body is a reaction to decrease the heat caused by prolonged exercise at high relative humidity (RH). This situation may cause an increase in pulse rate (PR), body temperature (BT), and blood lactic acid (BLA) workout. Objective: This study aimed to prove that a RH of 40% better than a RH of 50% and 60% RH in inhibiting the increase of PR, BT, and BLA during exercise. Methods: The study was conducted on 54 samples randomly selected from the IKIP PGRI Bali students. The samples were divided into three groups, and each group was given cycling exercise with a load of 80 Watt for 2 x 30 minutes with rest between sets for five minutes. Group-1 of cycling at 40% of RH, Group-2 at a RH of 50%, and the Group-3 at a RH of 60%. Data PR, BT, and BLA taken before and during exercise. The mean difference between groups before and during exercise were analyzed by One-way Anova and a further test used Least Significant Difference (LSD). Significance used was ? = 0.05. Results: The mean of PR during exercise was significantly different between groups with p = 0.045, the mean of BT during exercises was significantly different between groups with p = 0.006, and the mean of BLA during exercises was significantly different between groups with p = 0.005 (p
ESTROGEN RECEPTORS OF HAIRS BLACKS AND WHITES Laswati, Hening; Pangkahila, Jahja Alex
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

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Background: Aging is termed as same as degenerative process, in which all part of tissue organs retarted the microstructure either macrostructure, forming and function even the colour, including black hair change to white hair. Several researchers have been recommended that estrogen hormone be able ease black to white hair, but hormone without any presenting of receptor won’t be work properly. The main aim of this study were to determine amount of estrogen receptor contents in famales and males black and white hairs included the microscopically structure. Method: Twelve females and males there were 50 -56 years old each pairs black and white head hairs were plucked along with follicles. This estrogen receptors analyzed using radioreceptor binding assay there were 5mm eah hair follices including the root cutted and each pair put its in 2 ml glass tube already filled in with 500 µl 125I-estradiol and incubated in 37oC for 3 hrs. Following times were over the tube flushed twice carefully the hair won’t be flushed. Then count by putting in the gamma counter chamber for 1 minute each. The values that shown in the monitor as CPM (count per minute), recorded as receptor of estradiol. Results: Mean (±SD) sum estrogen receptor in females black and white hairs were 479.3 ± 37.5 and 387.7 ± 33.0, but significantly decreased in male black hair was 316.9±17.8 and 274.0 ± 19.8. All those pairs significantly different either female black and white hairs or male black and white hair and also significantly different among groups. Conclusion: The lowest estrogen receptors recorded in male white hairs and microstructure decreasing of melanin contents.
SPILANTHES ACMELLA AND PHYSICAL EXERCISE INCREASED TESTOSTERONE LEVELS AND OSTEOBLAST CELLS IN GLUCOCORTICOID-INDUCED OSTEOPOROSIS MALE MICE Laswati, Hening; Subadi, Imam; Widyowati, Retno; Agil, Mangestuti; Pangkahila, Jahya Alex
BALI MEDICAL JOURNAL Vol 4 No 2 (2015)
Publisher : BALI MEDICAL JOURNAL

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Background: Glucocorticoid-induced osteoporosis is leading cause of secondary osteoporosis by decreasing formation activity and increasing resorption activity. Spilanthes acmella, is one of Indonesia medicinal plants that contain of polyphenol and flavonoids. Previously in vitro study showed that buthanol and water fraction from this plant have increased alkaline phosphatase that known as marker of bone formation. The objective of this study to analyze the effect of Spilanthes acmella and physical exercise in increasing testosterone and osteoblast cells of femoral’s trabecular glucocorticoid-induced osteoporosis male mice. Method: This study using a posttest control group design, 36 male healthy mice (5 months old) were randomizely devided into 6 groups, there are : 1.Healthy control group (without induction dexamethaxone), 2.Osteoporosis groups (induction with dexamethaxone without treatment), 3.Positive control receive suspension alendronat, 4.70% Ethanol extract of Spilanthes acmella group, 5.Combination group of 70% extract ethanol of Spilanthes acmella and exercise, and 6.Exercise group (walking using mice treadmill 10m/minute, 5-12 minutes 3 times a week). All of the intervention were given for 4 weeks. The serum levels of testosterone were determined using immunoserology (ELISA) and osteoblast cells were determined histomorphometry by light microscopy. All statistical test were carried out using SPSS 23 and statistical significance was set at p
Tomato (Lycopersicum commune) Juice and Physical Exercise Increase Number of Neurons and ER? expression in Post-Ovariectomy Rats Brain Laswati, Hening; Subadi, Imam; Andriana, Meisy; Kurniawati, Patricia Maria; Pangkahila, Jahya Alex
BALI MEDICAL JOURNAL Vol 5 No 3 (2016)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Estrogen deficiency condition can degrade the quality of life, decline in cognitive function will be more severe trough age. Phytoestrogen compounds can be found in pegaga leaf extract, tomatoes, and papaya is an easy and inexpensive way to increase estrogen levels in post menopause women through extra gonadal estrogen induction. Therefore, the aims of this study were to examine the effect of tomato juice, physical exercise, and combination of these treatments on promoting neurons and ER? expression in somatosensory cortex that contribute to cognitive function of post-ovariectomy rats. Method: Twenty-eight female healthy Wistar rats (Rattus novergicus), 8-10 weeks old, from Laboratory of Biochemistry, Faculty of Medicine Airlangga University include in this experiment. The animals were housed in the animal-care facility with ad libitum food and water. The temperatur was maintained at 18°C-24°C. The treatments were done 2 weeks after ovariectomy. Tomato were made in Laboratory of Pharmacognocy and Phytochemistry, Faculty of Pharmacy, Airlangga University, from inner part of the tomato fruits (mucous like substance) with freeze dry method (-40°C). Results: The weight of white rat Rattus norvegicus post ovariectomy in this study was between 133-170 gram with a mean weight 154.32 ± 9.72 gram. Hematoxylin/eosin staining showed neuronal deficit in the control rats brain. In figure 1, the tomato group showed the largest of neurons number (145.43 ± 17.728), followed the combination group (140.57 ± 22.449), the exercise group (136.86 ± 23.104) and the smallest number in the control group (96.43± 28.965). Four weeks after treatments the number of neurons increased significant in the tomato group (p=0.001), exercise group (p=0.004) and combination group (p=0.002) from the control group. This study showed no significant different between tomato and exercise group (p=0.500), tomato and combination group (p=0.701) and between exercise and combination group (p=0.769). Conclusions: In conclusion, our data demonstrated that post ovariectomy rats showed deficit numbers of neurons and decreased ER? in the somatosensory cortex. Treatment with physical exercise, tomato juice, and combination of these treatments increased the number of neurons and ER? expression in the somatosensory cortex.
Co-Authors - Yuniar ., Zenitalia A Bagiada A.A.G.P. Wiraguna adiartha g Adiputra, IN. Adiputra, LMI. Sri Handari Adiputra, LMIS. Handari Adnyana Manuaba Agus Eka Darwinata AL UM ANISWATUN KHASANAH Alprodit Galatang Anak Agung Gede Sugianthara and I. B. Putra-Manuaba Andriana, RA Meisy Ariati, N.N. Bagus Komang Satriyasa Bambang Widayanto Benediktus Nahak Boedi Prihatini Yenniastoeti Catarina Budi P Chandra, Aji B. Christine Icasia Cornalius O. Lenati D A inten Delly Dahlia Dian Andrieany Husodo Dian Damayanti Dianika Putri Puspitasari Edelweishia, Melissa Eko Wibowo Emiliana Mariyah F. W Wagey Fatqur Rochman Ferbian M. Siswanto, Ferbian M. Ferbian Milas Siswanto Gimbar Adiputra Griadi, IP. Adiartha Hapy Ardiaviandaru Siamy HARA, MARIA KARERI Hening Laswati Putra Heny Widiyowati S I Dewa Ayu Inten Dwi Primayanti I Dewa Putu Sutjana I Gede Widhiantara I Gede Widjanegara I Gusti M. Aman I Gusti Made Aman I Gusti Ngurah Nala I Ketut Suada I Made Ady Wirawan I Made Bakta I Made Jawi I Made Krisna Dinata I MADE MULIARTA . I Nengah Sandi I Nyoman Adi Putra I Nyoman Adiputra I Nyoman Arsana I Nyoman Mangku Karmaya I Nyoman Nuada I Putu Adiartha Griadhi I Putu Agus Budi Sudarsana I Putu Gede Adiatmika I Wayan Rasmen Adikusuma I Wayan Sugianta I Wayan Suryawan I Wayan Weta I.B. Adnyana Manuaba I.G.P Surya Ida Bagus Ngurah Ida Sri Iswari IKW. Suryawan Imam Subadi, Imam IN. Adiputra indah adiputra Indah Pramita Indra Lesmana IP. Adiartha Griadi Irtanto, Okky Iswita Ariestianti Jasmine Kartiko Pertiwi K tirtayasa Kadek Tresna Adhi Ketut Tirtayasa Klaudius H. M. Meti Komang Ayu Tri Widhiyanti La Ode Muhammad Gustrin Syah Leandra Erdina Usmany LMI. Sri Handari Adiputra LMIS. Handari Adiputra Luh Made Indah Luh Made Indah Sri Handari Adiputra Luh Putu Ratna Sundari M Sarapung M. Ali Imron M. Irfan Made Oka Negara Mangestuti Agil Manuaba, I.B.P MB. Redy Utama Moh Ali Imron Mohammad Syahroni Muh Irfan Muh. Ali Imron Muh. Irfan Muh. Irfan Muhammad Irfan Muhammad Irfan Muhammad Irfan Mutiah Munawarah N. Adiputra N. Adiputra N. Sandi, N. Ni Ketut Dewita Putri Ni Komang Ayu Juni Antari Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Widarini Ni Putu Widya Astuti Ni Wayan Tianing Nurmalia Fitria Ningrum NW. Tianing Nyoman Suarjana Oktovianus Fufu Oktovianus Fufu Pamella Haryanto Pangkahila, Evangelista Maria Patricia Maria Kurniawati Periskila Abigail Prof. Dr. I Nyoman MS SH. Sirtha Putri Mariani, Putri R. A.T. Kuswardhani Regina Sesilia Noy Retno Widyowati Risha Catra Pradhany Rita Maria Rolles Nixon Palilingan Romy Kamaluddin Ronald Suryaprawira S. Indra Lesmana S. Indra Lesmana S. Indra Lesmana Santi Bery Hastuti Sugijanto - Sugijanto - Suryawan, IKW. Susy Purnawati Sutajaya, I.M. Syahmirza Indra Lesmana Tarnajaya, Karnia Taufan Hendra Wirawan Tianing, NW. Tirtayasa, K. Ursula Sulistiani Utama, MB. Redy Valentine Retny Shiajanto Weta, IW. Widianti, I Gusti Ayu Wijaya Surya, I Gede Ngurah Harry Wimpie I Pangkahila Wirawan, IMA. Yoselyn Gunawan Yuliana Ratmawati