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All Journal Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Universa Medicina Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Bioscientia Medicina : Journal of Biomedicine and Translational Research Science Midwifery Current Research on Biosciences and Biotechnology eJournal Kedokteran Indonesia Makara Journal of Health Research Journal of Global Pharma Technology
Gita Pratama
Departemen Kebidanan Dan Penyakit Kandungan, Fakultas Kedokteran, Universitas Indonesia, Rumah Sakit Cipto Mangunkusumo, Jakarta; Human Reproductive, Infertility And Family Planning Research Center, Indonesia Medical Education And Research Institute
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Journal of Global Pharma Technology

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Wharton’s Jelly Derived Mesenchymal Stem Cells: A Comparison Study in Preterm vs. Term Deliveries and in FBS vs. PRP vs Mesencult Culture Media Gita Pratama
Journal of Global Pharma Technology Volume 12 Issue 06 (2020) June 2020
Publisher : Journal of Global Pharma Technology

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Abstract

Background: Use of human mesenchymal stem cells (MSCs) for therapy in the field of degenerative medicine has been extensively studied. One of the potential sources of MSC which is easily available is from the human umbilical cords Wharton’s jelly of the newborn baby. Wharton's jelly derived MSCs (WJ-MSCs) from full-term labor has been isolated and differentiated, but very limited data came from preterm labor. This study aimed to compare the proliferation and differentiation characteristics between human WJ-MSCs from preterm and term labor using xeno-free culture media, particularly platelet rich plasma (PRP). Methods: WJ-MSCs from preterm and term deliveries was cultured using culture medium supplemented with 10% PRP or MSC or FBS culture media. Cells were cultured until the fifth passages. Cumulative cell numbers and differentiation capacity of the MSCs was tested according to International Society for Cellular Therapy (ISCT) criteria. Results: The preterm WJ-MSCs grown in culture media is not significant differences with WJ-MSCs derived from full-term labor, but have higher number of cell populations. WJ-MSCs are able to differentiate into osteocyte, chondrocyte and adipocyte., and xeno-free media can be used to replace FBS. WJ J-MSCs from preterm labor can be used as a source for mesenchymal stem cells. Conclusion: WJ-MSCs collected from preterm samples to have a better proliferation rate compared to term samples, while possessing equal differentiation capacity. In addition, PRP can replace FBS as it a xeno-free material for WJ-MSCs production that required for regenerative medicine. Keywords: Mesenchymal stem cells, Wharton’s jelly, Preterm labor, Proliferation, Differentiation.
Co-Authors Abdullah, Iftikar Achmad K. Harzif Achmad K. Harzif Achmad Kemal Achmad Kemal Harzif Ade P.R. Simaremare Agustina, Elizabeth Aldri Frinaldi Ampri, Irfan Arieqal Hatta Andon Hestiantoro Angeline, Wiedya Kristianti Aprilia Asthasari Siregar Ario Legiantuko Asmarinah Bagastoro, Aswan Budi Wiweko Caroline G. Puspita Christine Verawaty Sibuea Christopher Yo, Edward Eva Zakiyah Faradina, Anindita Abigail Fasha, Iqbal Ferry Sandra Ginting, Kharnis Marsha Madora Hari Kurniawan, Riyan Hartanto Bayuaji Herbert Situmorang Herbert Situmorang I. Putu G. Kayika Iftikar Abdullah Imelda Rosalyn Sianipar Indah S. Widyahening Inna Rahmawati Iqbal Fasha Irzanti, Alisha Nurdya Kanadi Sumapradja Kartiwa Hadi Nuryanto Kayika, I. Putu G. Kemal, Achmad Kuara , Glenessa Laila Nuranna Laila Nuranna Legiantuko, Ario Lestari, Silvia Werdhy Lusiana, Nadya Mila Maidarti Muharam, R mutiara mutiara Nuraditya, Robby Nurdya Irzanti, Alisha Prasetya, David Eka Prini Diandara Garinasih R. Muharam Raden Muharam Natadisastra Radiana Dhewayani Antarianto Rahmawati, Inna Raymond R. Tjandrawinata Retno Asti Werdhani Retno Lestari Retno Lestari Robby Nuraditya Samosir, Sarah Christina Samuel Febrian Wijaya Saraswati, Jaya Silaen, Rachel Teodora Silvana, Vita Silvia Werdhy Lestari Sitanggang, Ervina Julien Talya, Natasha Trinovita Andraini Tyagitha, Galuh Anindya Wijaya, Samuel Febrian Yana, Hiqmah Yusi Yanfaunas, Atika Mahira Yanfaunnas, Atika Mahira Yo, Edward Christopher