Garuda - Garba Rujukan Digital

Kombinasi Pupuk Nanosilika dan NPK Terhadap Pertumbuhan Tanaman Jagung (Zea mays L var. pioneer 21) Oktivani D.P. Hayati; Erma Prihastanti; Endah D. Hastuti
Jurnal Biologi Papua Vol 11, No 2 (2019)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Maize is one of food commodity in Indonesia. Maize pioneer 21 is a kind of high-yielding maize variety in Indonesia. One of the way able to be conducted to improve growth of maize plant is with fertilizing combination of nanosilica and NPK. This research aim to know the influence of fertilizing combination of nanosilica and NPK toward improving growth of maize plant and to know optimal concentration of combination nanosilica fertilizer and NPK in corn plants growth. Nanosilica is fertilizer that contains micronutrients Si while the NPK fertilizer contains macro nutrients NPK. Research conducted with Completely Ramdomized Design (CRD) with 6 combination factor: P0 (control), P1 (100% nanosilica), P2 (75% nanosilica + 25% NPK), P3 (50% nanosilica + 50% NPK), P4 (25% nanosilica + 75% NPK) and P5 (100% NPK) each has 3 replications. Parameter perceived by hat is high of plants, high of leaf, amount of leaf, wet heavy of maize plants and dry heavy of maize plant. Data to be analysed with Analysis of variance (ANOVA) level of signification 95%, continued by Duncan Multiple Range Test (DMRT) level of signification 95%. The results showed that the combination of nanosilica fertilization and NPK significantly affected the wet weight and dry weight of the Maize P-21. The optimal combination for the growth of Maize P-21 is the treatment of P4 (25% nanosilica+ 75% NPK). Key words: Zea mays L var. pioneer 21; combination; fertilizing; nanosilica; NPK

In-vitro Callus Development and The Bioactive Compounds of Tomato (Lycopersicon esculentum Mill.) Yulita Nurchayati; Erma Prihastanti; Rini Budihastuti
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21565

AbstractBioactive compounds in tomatoes can be produced through the development of callus culture. This study aimed to investigate callus development and observe bioactive compounds and antioxidant activities in explants and callus. The cotyledon and hypocotyl from the sprouts were induced to form callus on Murashige and Skoog (MS) medium supplemented with NAA 2.5 mg/L combined with kinetin 0.5 mg/L and 2,4 D 1 mg/L. All parts of seedling and callus were analyzed for their bioactive compounds and antioxidant activity using Spectrophotometer UV-Vis, whereas the other bioactive compounds were identified by Gas Chromatography-Mass Spectrophotometry. This research applied a Completely Randomized Design with sample sources of tomato sprout and callus from cotyledon and hypocotyl, with 3 replicates. The result showed that friable callus was able to be developed from both explants through the addition of NAA-Kin to MS medium. The three compounds were observed in callus and all explants. These calluses produced high antioxidant compounds from their pigments and ascorbic acid. The metabolites will be analyzed according to the perspective of their role. Major groups of compounds from GC-MS are dominated by hydrocarbons. Callus culture has a potential as the source of bioactive compounds.AbstrakSenyawa bioaktif pada tomat dapat diproduksi melalui kultur kalus, Penelitian ini bertujuan untuk mengkaji perkembangan kalus dan mengobservasi jenis senyawa bioaktif serta aktivitas antioksidan pada eksplan dan kalus. Kotiledon dan hipokotil dari kecambah diinduksi membentuk kalus di dalam medium MS dengan penambahan NAA 2,5 mg/L dan kinetin 0,5 mg/L, maupun 2,4 D 1 mg/L tunggal. Semua bagian kecambah dan kalus dianalisis kandungan senyawa bioaktif dan aktivitas antioksidannya dengan menggunakan Spektrofotometer UV-Vis. Sementara itu, senyawa bioaktif lainnya diidentifikasi dengan menggunakan Kromatografi Gas-Spektrofotometer Massa. Penelitian ini dilaksanakan dengan Rancangan Acak Lengkap dengan faktor sumber eksplan : kecambah, kalus yang berasal dari kotiledon maupun dari hipokotil disertai 3 ulangan. Hasil penelitian menunjukkan bahwa kalus remah dapat berkembang dari semua bagian kecambah pada medium MS dengan penambahan NAA dan kinetin. Terdapat tiga senyawa yang diobservasi baik pada kalus maupun sumber eksplannya. Kalus tersebut menghasilkan senyawa antioksidan yang tinggi, berdasarkan dari kandungan pigmen dan dari asam askorbat. Metabolit-metabolit tersebut akan dianalisis lebih lanjut terhadap peranannya. Kelompok senyawa yang terbanyak dari hasil GC-MS didominasi oleh hidrokarbon. Kultur kalus memiliki potensi sebagai sumber senyawa bioaktif tanaman.

In-vitro Callus Development and The Bioactive Compounds of Tomato (Lycopersicon esculentum Mill.) Yulita Nurchayati; Erma Prihastanti; Rini Budihastuti
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21565

AbstractBioactive compounds in tomatoes can be produced through the development of callus culture. This study aimed to investigate callus development and observe bioactive compounds and antioxidant activities in explants and callus. The cotyledon and hypocotyl from the sprouts were induced to form callus on Murashige and Skoog (MS) medium supplemented with NAA 2.5 mg/L combined with kinetin 0.5 mg/L and 2,4 D 1 mg/L. All parts of seedling and callus were analyzed for their bioactive compounds and antioxidant activity using Spectrophotometer UV-Vis, whereas the other bioactive compounds were identified by Gas Chromatography-Mass Spectrophotometry. This research applied a Completely Randomized Design with sample sources of tomato sprout and callus from cotyledon and hypocotyl, with 3 replicates. The result showed that friable callus was able to be developed from both explants through the addition of NAA-Kin to MS medium. The three compounds were observed in callus and all explants. These calluses produced high antioxidant compounds from their pigments and ascorbic acid. The metabolites will be analyzed according to the perspective of their role. Major groups of compounds from GC-MS are dominated by hydrocarbons. Callus culture has a potential as the source of bioactive compounds.AbstrakSenyawa bioaktif pada tomat dapat diproduksi melalui kultur kalus, Penelitian ini bertujuan untuk mengkaji perkembangan kalus dan mengobservasi jenis senyawa bioaktif serta aktivitas antioksidan pada eksplan dan kalus. Kotiledon dan hipokotil dari kecambah diinduksi membentuk kalus di dalam medium MS dengan penambahan NAA 2,5 mg/L dan kinetin 0,5 mg/L, maupun 2,4 D 1 mg/L tunggal. Semua bagian kecambah dan kalus dianalisis kandungan senyawa bioaktif dan aktivitas antioksidannya dengan menggunakan Spektrofotometer UV-Vis. Sementara itu, senyawa bioaktif lainnya diidentifikasi dengan menggunakan Kromatografi Gas-Spektrofotometer Massa. Penelitian ini dilaksanakan dengan Rancangan Acak Lengkap dengan faktor sumber eksplan : kecambah, kalus yang berasal dari kotiledon maupun dari hipokotil disertai 3 ulangan. Hasil penelitian menunjukkan bahwa kalus remah dapat berkembang dari semua bagian kecambah pada medium MS dengan penambahan NAA dan kinetin. Terdapat tiga senyawa yang diobservasi baik pada kalus maupun sumber eksplannya. Kalus tersebut menghasilkan senyawa antioksidan yang tinggi, berdasarkan dari kandungan pigmen dan dari asam askorbat. Metabolit-metabolit tersebut akan dianalisis lebih lanjut terhadap peranannya. Kelompok senyawa yang terbanyak dari hasil GC-MS didominasi oleh hidrokarbon. Kultur kalus memiliki potensi sebagai sumber senyawa bioaktif tanaman.

Effect of Corona Discharge Plasma Radiation on the Viability of True Shallot Seeds (Allium cepa L. var. aggregatum) Imam Firmansyah; Erma Prihastanti; Sri Widodo Agung Suedy; Zaenul Muhlisin; Arif Surahman
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 13, No 2 (2024): June 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i2.485-492

TSS (True Shallot Seed) need to be developed to address the quality and quality of shallot seeds. The seeds, however, still has constraints on its viability and germination. The objective of this study was to investigate the effect of corona discharge plasma radiation on the viability of true shallot seeds. The research was conducted in March - April 2023 at the Plasma Laboratory, Faculty of Science and Mathematics, Diponegoro University, and the germination test experiments at the the Agricultural Technology Research and Assessment Installation (IP2TP Ungaran). The experiment was designed completely randomized with 6 treatments of radiation time consisting of P0 (without radiation), P1 (5 min), P2 (10 min), P3 (15 min), P4 (20 min), and P5 (25 min). All treatments were carried out with 5 replications. The collected data were processed using ANOVA and then continued with the DMRT test. The results showed that the corona plasma radiation treatment for 15-25 min affected the parameters of germination, germination rate, seed growth rate, vigor index, seed uniformity, and sprout length. Keywords: Plasm, Radiation, Shallots, TSS.

Respon Anatomi Daun Adam Hawa (Rhoeo discolor) dan Pucuk Merah (Syzygium oleana) terhadap Polutan CO di Kecamatan Tembalang dan Banyumanik Kota Semarang Monika Heti Nuryana; Sri Darmanti; Erma Prihastanti
Jurnal Ilmu Lingkungan Vol 22, No 5 (2024): September 2024
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.22.5.1184-1193

Sebagai organisme yang bersifat sessile, tumbuhan dapat merespon perubahan lingkungan, salah satunya adalah pencemaran udara. Pencemaran udara dihasilkan dari kendaraan bermotor berupa gas karbon monoksida (CO), sulfur dioksida (SO2), nitrogen oksida (NOx), dan volatile organic compounds (VOCs). Kontak polutan dengan daun menyebabkan akumulasi polutan pada jaringan mesofil daun. Selanjutnya akan terjadi respon anatomi daun, antara lain adalah peningkatan jumlah sel stomata, penurunan panjang dan lebar celah stomata, penurunan lapisan kutikula serta penurunan tebal mesofil daun. Penelitian ini bertujuan untuk mengkaji respon anatomi daun adam hawa (Rhoeo discolor) dan pucuk merah (Syzygium oleana) terhadap polutan CO di Kecamatan Tembalang dan Banyumanik Kota Semarang. Tanaman diletakkan pada lokasi dengan kadar polutan CO yang termasuk dalam kategori baik, sedang, dan tidak sehat. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dua faktor yaitu kategori kadar CO dan jenis tanaman. Data kuantitatif dianalisis menggunakan ANOVA dan uji lanjutan DMRT pada taraf kepercayaan 95%. Parameter yang diamati untuk menunjukkan respon tanamaan terhadap kategori kadar CO yang berbeda adalah kerapatan stomata, lebar celah dan panjang stomata, tebal kutikula, dan tebal mesofil daun. Respon anatomi daun tanaman R. discolor maupun S. oleana terhadap polutan CO dapat ditunjukkan melalui penurunan lebar celah stomata dan penipisan lapisan kutikula daun.

Kandungan Pigmen Fotosintetik dan Pertumbuhan Vegetatif Tanaman Kedelai (Glycine max L. Merr.) var. Grobogan Pada Tingkat Ketersediaan Air yang Berbeda Fachrezi, Muhammad Luthfi; Darmanti, Sri; Saptiningsih, Endang; Prihastanti, Erma
Buletin Anatomi dan Fisiologi Volume 8, Nomor 2, Tahun 2023
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.8.2.2023.154-160

Kedelai merupakan sumber protein yang murah sehingga permintaan tinggi, tetapi produksi kedelai di Indonesia rendah. Tanaman kedelai umumnya ditanam di sawah tadah hujan sehingga rawan cekaman kekeringan dan menyebabkan produksi rendah. Penelitian ini menggunakan metode ekperimental, bertujuan mengkaji pengaruh tingkat ketersediaan air terhadap kandungan pigmen fotosintetik daun dan pertumbuhan vegetatif tanaman kedelai [Glycine max (l.) Merr.] varietas Grobogan. Penelitian dilakukan di kebun percobaan dan laboratorium Struktur dan fungsi tumbuhan Departemen biologi FSM UNDIP, menggunakan Rancangan Acak Lengkap (RAL) satu faktor berupa tingkat ketersediaan air yang berbeda yaitu 100%, 75%, 50%, dan 25% kapasitas lapang. Data dianalisis dengan analisis sidik ragam One Way ANOVA, dilanjutkan dengan Uji DMRT dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa penurunan persentase ketersediaan air tidak memengaruhi kandungan pigmen fotosintetik daun kedelai. Penurunan persentase ketersediaan air menurunkan pertumbuhan tajuk, untuk jumlah daun mulai turun pada ketersediaan air 25%, tinggi tanaman pada 75%, bobot tajuk pada 50%. Ketersediaan air paling optimal bagi panjang akar dan bobot akar adalah 75%. Soybeans are a cheap source of protein, so demand is high, but soybean production in Indonesia is low. Soybean crops are generally planted in rainfed rice fields, so they are prone to drought stress and cause low production. This study used an experimental method, aimed to examine the effect of water availability on the photosynthetic pigment content of leaves and vegetative growth of soybean plants [Glycine max (l.) Merr.] Grobogan variety. The research was conducted in the experimental garden and laboratory Plant structure and function of the Department of Biology FSM UNDIP, using a one-factor Complete Randomized Design (RAL) in the form of different levels of water availability, namely 100%, 75%, 50%, and 25% field capacity. The data was analyzed by One Way ANOVA fingerprint analysis, followed by DMRT Test with a confidence level of 95%. The results showed that the decrease in the percentage of water availability did not affect the photosynthetic pigment content of soybean leaves. A decrease in the percentage of water availability decreases the growth of the header, for the number of leaves begins to fall at water availability 25%, plant height at 75%, header weight at 50%. The most optimal water availability for root length and root weight is 75%.

Bioconcentration Factor of Avicennia marina (Forsk.) and Rhizophora mucronata (Lamk.) Inhabiting Coastal Area of Semarang City on Cadmium Hastuti, Endah Dwi; Izzati, Munifatul; Prihastanti, Erma
Biota Vol 11 No 1 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/

Mangrove plants are expected to act as bioaccumulator for heavy metals in the coastal area. One of heavy metals with great potential risk to the environment is Cadmium (Cd). This research aimed to study the concentration of Cd in the sediment, water, and mangrove organs, as well as to analyse the bioconcentration factor of mangrove plants in the coastal area of Semarang City. This research was carried out through experimental survey. The survey was carried out to a specified environment criteria which could be considered as experiment setting, including the growing location and mangrove species. The growing location including the shore and pond area while mangrove species using Avicennia marina and Rhizophora mucronata. The parameters are Cd level in root, leaf, water and sediment. Samples of Cd was taken from leaf and root as well as water and sediment under mangrove stands growing in the shore and pond areas, then the sample was analyzed using Atomic Absorbtion Spectrophotometer (AAS) method. The data was analyzed using ANOVA and further testing using DMRT. The result suggested that Cd accumulation was much higher in the pond area, both in the water and sediment. In addition, Cd concentration in mangrove organs showed similar behaviour. Bioconcentration factor analysis suggests that A. marina and R. mucronata are potential as bioaccumulator of Cd based on BCF value more than 1, comprising the index up to 20.333 for A. marina and 24.866 for R. mucronata.

Pertumbuhan dan Kandungan Klorofil Mangrove Rhizophora Stylosa Griff pada Salinitas yang Berbeda Hastuti, Endah; Niti, Mahatma Narendra; Prihastanti, Erma
Buletin Anatomi dan Fisiologi Volume 9, Nomor 2, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.2.2024.193-198

Permasalahan yang dialami vegetasi mangrove adalah populasinya yang kian menyusut drastis. Pengujian pertumbuhan dan kandungan klorofil R. stylosa harus dilakukan agar proses rehabilitasi di alam liar bisa sesuai dengan kondisi salinitas yang ada. Penelitian ini dilakukan dengan Rancangan Acak Lengkap (RAL). Penanaman R. stylosa dilakukan menggunakan ember yang diberi larutan salinitas dengan variasi: 15 ppt, 20 ppt, 25 ppt, dan 30 ppt masing-masing tiga kali ulangan. Parameter penelitian adalah: diameter batang, tinggi batang, jumlah daun, luas daun, dan kandungan klorofil. Data dianalisis dengan ANOVA, apabila terdapat perbedaan nyata maka dilakukan uji DMRT dengan kepercayaan 95%. Hasil ANOVA menunjukkan bahwa tidak ada perbedaan nyata antar perlakuan pada pertumbuhan diameter batang, tinggi batang, jumlah daun, dan luas daun, namun terdapat kecenderungan hasil terbaik terdapat pada salinitas 15 ppt dengan diameter batang (1,68 mm2), tinggi batang (15,53 cm), jumlah daun (5,33), dan luas daun (1650,79 mm2). Hasil uji ANOVA pada kandungan klorofil menunjukkan bahwa terdapat perbedaan nyata antar perlakuan. Hasil uji DMRT menunjukkan bahwa kandungan klorofil paling tinggi terdapat pada salinitas 15 ppt (11,015 mg/L). The problem experienced by mangrove vegetation is that its population is decreasing drastically. Testing of the growth and chlorophyll content of R. stylosa must be carried out so that the rehabilitation process in the wild can be in accordance with existing salinity conditions. This research was conducted in a completely randomised design (CRD). Planting of R. stylosa was done using a bucket that was given a salinity solution with variations: 15 ppt, 20 ppt, 25 ppt, and 30 ppt each with three replications. The research parameters were stem diameter, stem height, number of leaves, leaf area, and chlorophyll content. Data were analysed by ANOVA, if there were significant differences then DMRT test was conducted with 95% confidence. ANOVA results showed that there were no significant differences between treatments on the growth of stem diameter, stem height, number of leaves, and leaf area, but there was a tendency that the best results were in 15 ppt salinity with stem diameter (1.68 mm2), stem height (15.53 cm), number of leaves (5.33), and leaf area (1650.79 mm2). ANOVA test results on chlorophyll content showed that there were significant differences between treatments. The DMRT test results showed that the highest chlorophyll content was found at 15 ppt salinity (11.015 mg/L).

Effect of Corona Discharge Plasma Radiation on the Viability of True Shallot Seeds (Allium cepa L. var. aggregatum) Firmansyah, Imam; Prihastanti, Erma; Suedy, Sri Widodo Agung; Muhlisin, Zaenul; Surahman, Arif
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 2 (2024): June 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i2.485-492

TSS (True Shallot Seed) need to be developed to address the quality and quality of shallot seeds. The seeds, however, still has constraints on its viability and germination. The objective of this study was to investigate the effect of corona discharge plasma radiation on the viability of true shallot seeds. The research was conducted in March - April 2023 at the Plasma Laboratory, Faculty of Science and Mathematics, Diponegoro University, and the germination test experiments at the the Agricultural Technology Research and Assessment Installation (IP2TP Ungaran). The experiment was designed completely randomized with 6 treatments of radiation time consisting of P0 (without radiation), P1 (5 min), P2 (10 min), P3 (15 min), P4 (20 min), and P5 (25 min). All treatments were carried out with 5 replications. The collected data were processed using ANOVA and then continued with the DMRT test. The results showed that the corona plasma radiation treatment for 15-25 min affected the parameters of germination, germination rate, seed growth rate, vigor index, seed uniformity, and sprout length. Keywords: Plasm, Radiation, Shallots, TSS.

Kandungan, Resorpsi N dan P serta Specifi c Leaf Area Daun Kakao (Theobroma cacao L.) pada Cekaman Kekeringan Erma Prihastanti; Soekisman Tjitrosemito; Didy Sopandie; Ibnul Qayim; Christoph Leuschner
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 1 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Plant nutrient absorptions depend on their environment and plants’ stage of development. Beside from soil, nutrient absorption also proceed through resorption in leaves. The resorption efficiency varies based on types of habitat. Drought stress also influences the efficiency of nutrient resorption, growth rate, and plant development. The purpose of this research was to study the change of nitrogen (N) and phosphorus (P) content and leaf resorption value of N and P, and specific leaf area of 6-years old cacao grown under drought stress. Research was carried out in a cacao plantation with 7-years old Gliricidia sepium Jacq. as shading trees. The results showed that drought stress with Troughfall Displacement Experiment (TDE) system did not affect the content and resorption level of N and P of cacao leaves. However, N resorption of cacao leaves tended to increase while P resorption decreased. Cacao plants experienced drought stress have the ability to resorp leaf N for 46.64-50.63%, leaf P for 47.98-58.40%, whereas, the control plants had the leaf N resorption of 45.05-52.97%, and leaf P resorption of 36.64-44.10%. Drought stress for 13 months on 6-years old cacao did not affect specifi c leaf area.

Title

Found 72 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 72 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 72 Documents
Search