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Effect of Subculture Frequency Toward Growth And Carotenoid Content from Tomato (Lycopersicon Esculentum Mill.) Callus Indriani, Reni; Prihastanti, Erma; Budihastuti, Rini; Nurchayati, Yulita
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5840

Abstract

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plants, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aimed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment.
Effect of Corona Discharge Plasma Radiation on the Viability of True Shallot Seeds (Allium cepa L. var. aggregatum) Firmansyah, Imam; Prihastanti, Erma; Suedy, Sri Widodo Agung; Muhlisin, Zaenul; Surahman, Arif
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 2 (2024): June 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i2.485-492

Abstract

TSS (True Shallot Seed) need to be developed to address the quality and quality of shallot seeds. The seeds, however, still has constraints on its viability and germination. The objective of this study was to investigate the effect of corona discharge plasma radiation on the viability of true shallot seeds. The research was conducted in March - April 2023 at the Plasma Laboratory, Faculty of Science and Mathematics, Diponegoro University, and the germination test experiments at the the Agricultural Technology Research and Assessment Installation (IP2TP Ungaran). The experiment was designed completely randomized with 6 treatments of radiation time consisting of P0 (without radiation), P1 (5 min), P2 (10 min), P3 (15 min), P4 (20 min), and P5 (25 min). All treatments were carried out with 5 replications. The collected data were processed using ANOVA and then continued with the DMRT test. The results showed that the corona plasma radiation treatment for 15-25 min affected the parameters of germination, germination rate, seed growth rate, vigor index, seed uniformity, and sprout length.  Keywords: Plasm, Radiation, Shallots, TSS.
Kandungan, Resorpsi N dan P serta Specifi c Leaf Area Daun Kakao (Theobroma cacao L.) pada Cekaman Kekeringan Prihastanti, Erma; Tjitrosemito, Soekisman; Sopandie, Didy; Qayim, Ibnul; Leuschner, Christoph
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 1 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.411 KB) | DOI: 10.24831/jai.v39i1.13191

Abstract

Plant nutrient absorptions depend on their environment and plants’ stage of development. Beside from soil, nutrient absorption also proceed through resorption in leaves. The resorption efficiency varies based on types of habitat. Drought stress also influences the efficiency of nutrient resorption, growth rate, and plant development. The purpose of this research was to study the change of nitrogen (N) and phosphorus (P) content and leaf resorption value of N and P, and specific leaf area of 6-years old cacao grown under drought stress. Research was carried out in a cacao plantation with 7-years old Gliricidia sepium Jacq. as shading trees. The results showed that drought stress with Troughfall Displacement Experiment (TDE) system did not affect the content and resorption level of N and P of cacao leaves. However, N resorption of cacao leaves tended to increase while P resorption decreased. Cacao plants experienced drought stress have the ability to resorp leaf N for 46.64-50.63%, leaf P for 47.98-58.40%, whereas, the control plants had the leaf N resorption of 45.05-52.97%, and leaf P resorption of 36.64-44.10%. Drought stress for 13 months on 6-years old cacao did not affect specifi c leaf area.
Co-Authors Afrazak Johansyah Agus Subagio Agus Subagyo Ahmad Fuad Masduqi Anitasari, Emi Arif Surahman Ariyanti Ariyanti Ariyanti Ariyanti Ayu Rahmawati Sulistyaningtyas C. J. Soegihardjo Chatarina Umbul Wahyuni Chory Praseptiana Christoph Leuschner Della Widya Puspita Della Widya Puspita Desy Wulan Triningsih Devi Ermawati Dhitya Arlan Bayu Martha Didie Soepandi Didy Sopandie Diwyacitta Prasasti Emi Anitasari Endah D. Hastuti Endah Dwi Astuti Endah Dwi Hastuti Endang Kusdiyantini Endang Saptiningsih Esti Meita Kridati Fajar Arianto Faradis Ulyah Fatkhu Zaimah Febiasasti Trias Nugraheni Fifit Yuniati Harmigita Putri Fitriani Hastuti, Endah Helmiyesi Helmiyesi I’anatushshoimah, I’anatushshoimah IBNUL QAYIM Ika Dyah Kumalasari Ika Puspitasari I Ilma Khaerasani Imam Firmansyah Imam Firmansyah Indriani, Reni Jinus Jinus Jumari Jumari, Jumari Junita Junita Kasiyati Kasiati Ken Qudsy Royana, Ken Qudsy Kuwati Kuwati Lily Nur Inda Sary Lutfiyatul Wahdah Mega Rizqi Utami Monika Heti Nuryana Much Azam Much Azam Mudlikatun Khasanah Muhammad Agus Muljanto Muhammad Luthfi Munifatul Izzati Munirotun Roiyana Ngadiwiyana M.Si. S.Si. Ningrum, Dyah Ayu Kusuma Nintya Setiari Niti, Mahatma Narendra Noor Laila Safitri Nurrahmah Azizah, Nurrahmah Oktivani D.P. Hayati Puji Hastuti Putri Zulaida Ningtyas Rafif Zuhair Muhammad Regina Cahya Cendekianesti Retno Robiatul Al Adawiyah Rini Budi Hastuti Rini Budi Hastuti Rini Budihastuti Robi’atul Asifah Sarjana Parman Sepsamli, Letus Silvana Tana Soekisman T SOEKISMAN TJITROSEMITO Sofian, Arif Ardianto Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sugiyatno Sugiyatno Sulastri Diana Silaban Surahman, Arif surur, Mukhammad akmal Susiani Purbaningsih, Susiani Tyas Rini Saraswati Ulva, Maria Wijaya, Panca Buana Winangsih Winangsih Yulita Nurchayati Zaenul Muhlisin Zaenul Muhlisin Zelly Fujianto, Zelly Zelly Fujiyanto, Zelly