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Journal : bionature

Potensi Perlakuan Awal Limbah Kulit Udang untuk Produksi Enzim Kitinase oleh Trichoderma virens pada Fermentasi Substrat Padat Rachmawaty, .; Madihah, .
bionature Vol 14, No 1 (2013): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.035 KB) | DOI: 10.35580/bionature.v14i1.1445

Abstract

Telah dilakukan perlakuan awal limbah kulit udang sebagai substrat untuk produksi enzim kitinase menggunakan Trichoderma virens pada fermentasi substrat padat.  Udang sebagai kelompok Crustacea kaya akan kandungan kitin yang digunakan untuk menginduksi produksi enzim kitinase.  Lima perlakuan awal kulit udang yang berbeda digunakan sebagai substrat untuk produksi enzim kitinase yaitu perlakuan fisik (pemanasan sinar matahari, oven, mikrowave dan pemasakan) dan kimiawi (5% (v/v) HCl dan 5% (w/v) NaOH).  Perlakuan awal kulit udang menggunakan mikrowave menghasilkan aktivitas enzim kitinase tertinggi (0.194 U/gds) dibandingkan dengan kontrol, pemasakan, oven, pemanasan sinar matahari, dan kimiawi dengan peningkatan masing-masing 2.9; 1.37; 1.2; 1.2; dan 41.3 kali lebih tinggi.  Dibandingkan dengan penelitian sebelumnya, menggunakan fermentasi substrat padat, Trichoderma virens menghasilkan aktivitas enzim kitinase lebih tinggi 4 kali dibandingkan dengan hasil yang menggunakan fermentasi cair terendam.
Indeks Keanekaragaman Makrozoobentos Sebagai Bioindikator Tingkat Pencemaran Di Muara Sungai Jeneberang Rachmawaty, .
bionature Vol 12, No 2 (2011): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.113 KB) | DOI: 10.35580/bionature.v12i2.1403

Abstract

Makrozoobentos living relative lived, so it was better used as a guide environmental quality, as always contact with the waste into their habitat. Bivalvia and gastropod the most widely used as bioindicator pollution because he lived. This study aimed to know the level of species diversity in the estuary in estuary of Jeneberang River, to know the abundance of mollusks and know the type of mollusks that dominate in estuary of Jeneberang River. This research was descriptive of how diversity in estuary of Jeneberang River. The results obtained based on the diversity index showed <1.0, showed estuarine of Jeneberang River including heavy polluted waters. Based on the dominance index gained value approaching zero, then there is no dominant species.
Pengaruh Bahan Pengikat Terhadap Sifat Kimia dan Organoleptik Produk Nugget Tempe Hartati, .; A.Faridah, Arsal; Rachmawaty, .
bionature Vol 10, No 2 (2009): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.749 KB) | DOI: 10.35580/bionature.v10i2.1363

Abstract

The diversification limitation of exploiting tempe as an alternative nugget raw materials, substitution of meat and fish, and also to optimalize the very abundance resource, so it is necessary to do the right processing to the alternative food resource, which meant to give an additional point. Produce a liked product, also the adjustment of technology, and the cost that reached by the society.          The aim of this reseach is to know the kind of glue material which can produce nugget tempe with the best chemical and organoleptic propertics. This research consit of 4 treatment; A1 (tempe nugget from bread powder), A2 (tempe nugget from tapioca), A3 (tempe nugget from sagu), A4 (tempe nugget maizena powder). Water convcentration analyse using oven method. Product was tested by organoleptic test (taste, smell, and tecsture).          The result on water concentration analyse, that the smallest water concentration shown by A2  about 161,55% between from treatment A1 abaout 191,75%, A3 abaout 180,02% and A4 182, 45%. The result on organoleptic the teste of A1 product is the most like (75 %) between A2, A3 and A4 treatment. The smell of A2, A3 and A4 is the must like (67 %). And the best tecsture is all treatment.The result of this research is expected to give benefit in supporting the effort saking the alternative nugget glue material by using tempe which owning a high nutrition value. This research it is expected to find a kind if nugget tempe with the good glue, good taste and high nutrition value           
hartono_a.madjid@unm.ac.idKomparasi Enzim Kitinase dari Beauveria bassiana galur Lokal Sul Rachmawaty, .
bionature Vol 10, No 2 (2009): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.528 KB) | DOI: 10.35580/bionature.v10i2.1360

Abstract

This study aims to determine the enzyme activity of chitinase B. bassiana strain found in South Sulawesi. Knowing the relationship between the activity of chitinase enzyme isolate-isolate B.bassiana strain in South Sulawesi with mortality grayak caterpillar (Spodoptera litura). This descriptive research by linking the enzyme activity of chitinase from Beauveria bassiana strain local South Sulawesi on mortality grayak caterpillar larvae (Spodoptera litura). Based on the research that has been done shows that the activity of the enzyme from South Sulawesi B.bassiana local strains showed differences in each isolate, isolates the origin Enrekang 7.15 units / ml, isolate origin Bantaeng 7.12 units / ml, 6.32 units of isolate origin Pinrang / mi and isolate the origin of Maros 6.2 units / ml. There is a relationship between enzyme activity with mortality rates of larvae of Spodoptera litura. The highest mortality is caused by the isolate origin Enrekang 86%, followed by the isolate origin Bantaeng 83%, 76% Pinrang origin isolates and isolate the origin of Maros 73%.
Karakterisasi Isolat-isolat Beauveria Bassiana Galur Lokal Sulawesi Selatan Rachmawaty, .
bionature Vol 9, No 2 (2008)
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v9i2.148

Abstract

Beauveria mildew is known as useful natural control agent, due to it’s infection potential agents for controlling insect. The range of its host covers broad range of insects order including Lepidoptera, Coleoptera, Hemiptera and Hymenoptera. In the past, the behavior of B.bassiana was identified based on morphological character, which is have weakness because of environmental effect so the fenotype performance does not manifest the genotype value. Another identification method can be used to complete morphological approach is identification based on physiological behavior (toxin production) and biochemical (protein and isoenzim) where the protein analysis and isoenzim, is order to minimize the influence of environment factor for identification. The research was conducted in Laboratorium Biology UNM, there is 11 isolates B.bassiana used in this research represent location and host. Isolates were analyzed by physiological and isoenzim using electrophoresis. The result of physiological character showed that out of 11 isolates characterized, there are isolates produced beauvericin, interestingly the rest isolates produced unknown on identified metabolites. Based on total band protein pattern and isoenzim showed that there are variation, isolates from the same area or district have more similarity characters than isolates from different area.
The Effect of The Addition of Mealworm Frass (Tenebrio Molitor) and Molasses on the Increasing of the Proximate Value of White Oyster Mushroom (Pleurotus Ostreatus) Mentari Jelita Putri; Andi Faridah Arsal; Halifah Pagarra; Rachmawaty Rachmawaty; Andi Asmawati Asiz; Alimuddin Ali; Abd Muis; Muhammad Junda; Yasser Abd Djawad; Oslan Jumadi
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.44639

Abstract

This study aims to determine the effect of the addition of mealworm frass and molasses on the proximate value of white oyster mushrooms. This type of research was an experiment using a completely randomized design (CRD) consisting of four treatments with three replications each. Test parameters include water content, ash content, crude protein, crude fat and crude fiber. Proximate data on water content, ash content, crude protein, crude fat and crude fiber were analyzed using analysis of variance (ANOVA) and Tukey's follow-up test with a 95% confidence level. The results showed that treatment with frass and frass molasses had an effect on water content, ash content, crude protein, crude fat, and a decrease in fiber content. Therefore, it can be concluded that the addition of mealworm frass and molasses affects the proximate value of the resulting oyster mushrooms.Keywords: White Oyster Mushroom, Flour Caterpillar Frass, Molasses, Proximate.