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All Journal Biomedical Engineering Global Medical and Health Communication Science and Technology Indonesia Jurnal Ilmu Kedokteran dan Kesehatan Jurnal Kedokteran Meditek
Fanny Rahardja
Departemen Mikrobiologi Fakultas Kedokteran, Universitas Kristen Maranatha, Bandung, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Dentistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Journal : Biomedical Engineering

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Phytochemical assay, potential of antimalarial and antioxidant activities of green tea extract and its fractions Fanny Rahardja; Rita Tjokropranoto; Wahyu Widowati; Siska Lusiana; Adrian Suhendra; Susy Tjahjani; Iwan Budiman; Maesaroh Maesaroh; Nurul Fauziah
Biomedical Engineering Vol 1, No 1 (2014)
Publisher : Biomedical Engineering

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Abstract

Objective : The research objective was to evaluate the phytochemical compounds, the antioxidant and anti-malarial activity of green tea extract and its fraction. Methods: The antioxidant activity were measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity used in vitro laboratories prospective experimental design and completely randomized design, using ethanol extract, hexane, ethyl acetate, butanol fraction, and water fraction ofgreen tea in 7 levels of concentration compared to epicatechin (EC). The data were analyzed using linear regression analysis, and were continued by determined Inhibitory Concentration). The anti-malarial activity of green tea fractions were compared to artemisinin. The data were analyzed using probit analysis, and were continued by determined IC. Results: The results showed that the highest antioxidant activity (IC50) was the fraction of ethyl acetate 2,184 µg/ml. The anti-malarial activity IC50 50 of water and ethyl acetate fraction, ethanol extract and butanol fraction were 0,000090 µg/mL,0,003 µg/mL, 0,006 µg/mL, 0,011 µg/ml respectively.Conclusion: Extract and fractions of green tea have potential anti-malarial and antioxidant properties. Key Words: antioxidant; anti-malarial; green tea; free radicals
Phytochemical assay, potential of antimalarial and antioxidant activities of green tea extract and its fraction Fanny Rahardja
Biomedical Engineering Vol 1, No 1 (2015): BIOMEDICAL ENGINEERING
Publisher : Biomedical Engineering

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Abstract

Objective : The research objective was to evaluate the phytochemical compounds, the antioxidant and anti-malarial activity of green tea extract and its fraction. Methods : The antioxidant activity were measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity used in vitro laboratories prospective experimental design and completely randomized design, using ethanol extract, hexane, ethyl acetate, butanol fraction, and water fraction of green tea in 7 levels of concentration compared to epicatechin (EC). The data were analyzed using linear regression analysis, and were continued by determined Inhibitory Concentration50 (IC50). The anti-malarial activity of green tea fractions were compared to artemisinin. The data were analyzed using probit analysis, and were continued by determined IC50 .Result: The results showed that the highest antioxidant activity (IC50) was the fraction of ethyl acetate 2,184 µg/ml. The anti-malarial activity IC50of water and ethyl acetate fraction, ethanol extract and butanol fraction were 0,000090 µg/mL,0,003 µg/mL, 0,006 µg/mL, 0,011 µg/ml respectively.Conclusion : Extract and fractions of green tea have potential anti-malarial and antioxidant properties.
Co-Authors Adrian Suhendra Ardo Sanjaya Azis, Rizal Diana Krisanti Jasaputra Evan Christian Evan Christian, Evan Gerardo Vico Fernando Budiono Gunadi, Julia Windi Hadiprasetyo, Dhanar Septyawan Hanna Sari Widya Kusuma, Hanna Sari Widya Iwan Budiman Julia Windi Gunadi Maesaroh Maesaroh Mariska Elisabeth Ningrum, Siti Ratu Rahayu Nurul Fauziah Octavallen, Andri Rita Tjokropranoto Sabrina, Adilah Hafizha Nur Sarwono, Sylvie Sijani Prahastuti Siska Lusiana Susy Tjahjani Tanuwijaya, Farrell Teresa Liliana Wargasetia Wahyu Widowati WAHYU WIDOWATI Zahiroh, Fadhilah Haifa