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All Journal Indonesian Journal of Conservation Abdimas Lembaran Ilmu Kependidikan Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Sainteknol : Jurnal Sains dan Teknologi Jurnal Biologi dan Pembelajarannya (JBP) Journal of Biology Education Unnes Science Education Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Journal of Science Education Research Abdimas Dewantara JMM (Jurnal Masyarakat Mandiri) Biosaintifika: Journal of Biology & Biology Education Journal of Dedicators Community Floribunda Journal of Innovative Science Education Jurnal Pendidikan Bahasa dan Sastra Indonesia Kumawula: Jurnal Pengabdian Kepada Masyarakat Abdimas Toddopuli: Jurnal Pengabdian Pada Masyarakat Jurnal Abdi Insani Diseminasi : Jurnal Pengabdian kepada Masyarakat Bioeduca : Journal of Biology Education Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Prosiding Seminar Nasional Pascasarjana Proceeding of International Conference on Science, Education, and Technology Prosiding Seminar Nasional Biologi "Indonesian Journal of Devotion and Empowerment (IJDE) "

Amin Retnoningsih

Unknown Affiliation

Author-ID : 359893

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

Published : 92 Documents Claim Missing Document

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Articles

Title

Dua Setengah Abad Perkembangan Konsep jenis: Perjalanan Panjang yang Sarat Perdebatan Amin Retnoningsih
Floribunda Vol. 2 No. 1-8 (2002)
Publisher : PTTI

Two and half centuries of the species concepts development: a long time with fully discussion.

PENANDA MIKROSATELIT SEBAGAI PENCIRI JATI ”PLUS” Mariya Ulfa; Amin Retnoningsih; Endah Suwarni
Floribunda Vol. 4 No. 1 (2010)
Publisher : PTTI

Mariya Ulfa, Amin Retnoningsih & Endah Suwarni. 2010. Microsatellite Marker as Characterization of Teak “Plus”. Floribunda 4(1): 5–14.— The result of this study showed that all primers used were polymorphic allele. Locus Tg-AAG10 could detect polyploidy of teak accession J32 and J37 from Ngawi. The total of alleles detected was 8, with the average 6 alleles per locus. The highest amount was found in locus Tg- AAG10 (9 alleles). The genetic diversity parameter of Java teak “plus” using microsatellite marker showed high result and also could detect allele and specific genotype. Allele and specific genotype as teak fingerprint data could be detected by all three loci, so that all loci that were used in this research could be used as fingerprint of teak “plus” in Java.

CHARACTERIZATION AND EVALUATION OF MUSA ACUMINATA CULTIVARS IN INDONESIA BASED ON MICROSATELLITE MARKERS Amin Retnoningsih; Rita Megia; Alex Hartana
Floribunda Vol. 4 No. 1 (2010)
Publisher : PTTI

Amin Retnoningsih, Rita Megia & Alex Hartana. 2010. Characterization and Evaluation of Musa acuminata Cultivars in Indonesia Based on Microsatellite Markers. Floribunda 4(1): 21–32. — Difficulties in identifying Musa acuminata cultivars are caused by their large variations, numerous names and synonyms. The morphological characters provide limited information, therefore identifying cultivars could be difficult. In line with the difficulty to identify the cultivars, thus this study was aimed to verify taxonomic status, to identify and to reveal genetic relationships among banana accessions based on microsatellite markers. The DNA of 59 banana accessions which have morphologically been classified into AA and AAA genomic groups was analyzed using 12 primers. The results showed that only 8 primers producing discrete and repeatable alleles. The number of alleles per primer ranged from 5 for the MaCIR327b to 15 for the MaCIR108 and the Ma-1-139. The largest three of the MaCIR108 alleles included in length range of the B genomic alleles, whereas the other 12 alleles fell into length range of the A genomic alleles. Hence, the 11 accessions having alleles of the A and B genomes have been incorrectly classified into M. acuminata cultivars. They should be placed in hybrids species M. x paradisiaca. Forty eight accessions studied neither exhibited status change, nor possessed specific alleles to distinguish between AA and AAA genomic groups. Difficulties in separating AA from AAA may also as a result of the dosage effects of a microsatellite allele. Therefore, the accessions having maximum 2 alleles in each primer should be designated as AA or AAA genomic group. Due to the presence of 6 identical genotypes consisting of 12 accessions, there were detected only 42 genotypes resulted from analysis of the 48 accessions using the 8 primers. Genetic relationship analysis showed that genomic groups of those pure acuminata cultivars could not be differentiated significantly, though most of them tend to be clustered according to ploidy level.

HUBUNGAN KEKERABATAN FILOGENETIKA KULTIVAR PISANG DI INDONESIA BERDASARKAN KARAKTER MORFOLOGI Amin Retnoningsih
Floribunda Vol. 4 No. 2 (2011)
Publisher : PTTI

Amin Retnoningsih. 2011. Phylogenetic relationship of cultivated bananas in Indonesia based on morphological characters. Floribunda 4(2): 48–53. — Indonesia has high biodiversity of cultivated bananas. This research objective is reveal the phylogenetic relationship of cultivated bananas in Indonesia based on morphological characters. This research analyzed 2 groups which are consist of ingroup namely 9 banana accessions on genome groups AA, 9 AAA accession, 8 AAB accession, 8 ABB accession, and 2 BB accession, and the other group is outgroup which representated by 2 wild bananas. The method of this research usingCladistic program Phylogenetic Analysis Using Parsimony (PAUP) version 4.0, it used to produce phylogenetic trees. The study branches are tested by bootstrap 100 times. The results show that morphological characters have parallel homoplasm which can be seen from the low CI score by 0.275. Taxon tested are species and under species level (Musa spp). In general, there is no difference on changes of morphological characters in each clade. The clade consist of Boi accession (ABB), Klutuk Wulung (BB) and Klutuk (BB) separated from ingroup clade because they have differences of morphological characters from the others. They are showed by high bootstrap score: 98%. The Klutuk Wulung and Klutuk are predicted to have close phylogenetic relationship with the wild Musa balbisiana (BB).

PHYLOGENETIC RELATIONSHIPS OF INDONESIAN BANANA CULTIVARS INFFERED FROM TRNL-F INTERGENIC SPACER OF CHLOROPLAST DNA Amin Retnoningsih; Rita Megia; Alex Hartana
Floribunda Vol. 4 No. 8 (2014)
Publisher : PTTI

A phylogenetic relationship of Indonesian banana cultivars was conducted using trnL-F intergenic spacer of chloroplast DNA sequences. The ingroup was 39 banana accessions from various genomic groups in Indonesia consisting of 9 AA/AAA; 11 AAA; 2 BB; 13 AAB; and 4 ABB cultivars and 12 sequences of different taxa of Musa downloaded from GenBank consisting of 4 AA wild; 1 AA; 3 AAB; and 4 ABB cultivars. Two accessions from section Rhodoclamys and Australimusa were used as the outgroup. The 39 accessions of the ingroup and the 2 accessions of the outgroup were analyzed using primers of the trnL-F intergenic spacer. The results showed that sequence of the trnL-F region can clearly distinguish 40 banana accessions having the A-type chloroplast (AA wild; AA; AA/AAA; AAA; AAB; and ABB) from those 11 accessions having the B-type chloroplast (BB, AAB, and ABB). The A-type chloroplast accessions are most probably derived from female Musa acuminata, while the B-type chloroplast accessions may be derived from female M. balbisiana. However, the specific M. acuminata ancestor of each cultivar remains unidentified.

KARAKTERISASI AKSESI DURIAN LOKAL KOLEKSI HORTIMART AGRO CENTRE JAWA TENGAH MENGGUNAKAN PENANDA MOLEKULER INTER SIMPLE SEQUENCE REPEATS (ISSR) Ahmad Solikin; Amin Retnoningsih; Enni S. Rahayu
Floribunda Vol. 5 No. 7 (2017)
Publisher : PTTI

Ahmad Solikin, Amin Retnoningsih & Enni S. Rahayu. 2017. Characterization of Local Durian Accession Collected Hortimart Agro Centre, Central Java Using Molecular Markers Inter Simple Sequence Repeats (ISSR). Floribunda 5(7): 267–276. — The lack of morphological markers encourage the use of other markers that are considered more accurate, i.e molecular markers. The purpose of this study is to analyze the genetic diversity as well as determine the identity of the local durian accession collection of Hortimart Agro Centre, Central Java. Leaves of 41 durian accessions were isolated using CTAB method. DNA amplification was conducted with four primers of ISSR PKBT and the results were separated by agarose gel. Data were analyzed using NTSYSpc program version 2: 02. The results showed that DNA amplification using four primers of ISSR BKPT yielded 66 polymorphic bands. The results of the analysis showed that the genetic diversity of local durians of Hortimart collection is high because 100% of polymorphic DNA bands with variations of size 250–1500 bp. No local durian accession was found to have the same DNA100% profile, although four pairs of accessions were known to have a 96% high similarity. Five accessions, namely Petruk 2, Ontoseno, Semar, Arjuno, and Gondomono have specific DNA bands. The only accession of durian apart from the other group is Gondomono because it has only one band on ISSR PKBT 8 with size 750 bp which is not owned by other accession. However, dendrograms showed that no identities that are 100% equal so that eachDNA band profile can be the identity of each durian accession.Keywords: Durio zibethinus, molecular marker, genetic diversity.Ahmad Solikin, Amin Retnoningsih & Enni S. Rahayu. 2017. Karakterisasi Aksesi Durian Lokal Koleksi Hortimart Agro Centre Jawa Tengah Menggunakan Penanda Molekuler Inter Simple Sequence Repeats (ISSR). Floribunda 5(7): 267–276. — Keterbatasan ciri morfologi mendorong penggunaan penanda lain yang dipandang lebih akurat, yaitu penanda molekuler. Tujuan penelitian ini adalah menganalisis keanekaragaman genetika sekaligus menentukan identitas aksesi durian lokal koleksi Hortimart Agro Centre, Bawen, Jawa Tengah. Daun 41 aksesi durian diisolasi menggunakan metode CTAB. Amplifikasi DNA dilakukan dengan empat primer ISSR PKBT dan hasilnya dipisahkan melalui gel agarose. Data dianalisis menggunakan program NTSYSpc versi 2:02. Hasil penelitianmenunjukkan bahwa amplifikasi DNA menggunakan empat primer ISSR BKPT menghasilkan 66 pita polimorfik. Hasil analisis menunjukkan bahwa keanekaragaman genetika durian lokal koleksi Hortimart tergolong tinggi karena 100% pita DNA polimorfik dengan variasi ukuran 250–1500 bp. Tidak ditemukan aksesi durian lokal yang memiliki profil DNA100% sama, meskipun empat pasang aksesi diketahui memiliki kemiripan tinggi 96%. Lima aksesi, yakni Petruk 2, Ontoseno, Semar, Arjuno, dan Gondomono memiliki pita DNA spesifik. Satu-satunya aksesi durian yang terpisah dari kelompok lainnya adalah Gondomono karena hanya memiliki satu pita pada ISSR PKBT 8 dengan ukuran 750 bp yang tidak dimiliki aksesi yang lain. Meskipun demikian, dendrogram memperlihatkan tidak ada identitas yang 100% sama sehingga setiap profil pita DNA dapat menjadi identitassetiap aksesi durian. Kata kunci: Durio zibethinus, penanda molekuler, keanekaragaman genetika.

Co-Authors A.A. Ketut Agung Cahyawan W Abdul Latif Abdullah Muamar Aditya Marianti Ahmad Minanur Rohim Ahmad Solikin Aji Purwinarko, Aji Akbar, Bintang Faisal Alex Hartana Alex Hartana Aloysius Joko Susilo Amin Pujiati Andin Irsadi Andreas Priyono Budi Prasetyo Ani Rusilowati Annindya Ardiansari Aprilianingtyas Anggraeni, Aprilianingtyas Asi, Maolina Avif Intan Linawati, Avif Intan Awan Usy Syuru, Awan Usy Azfa, Zuraida Anissa Baihaqi, Imam Bambang Riyono Bambang Sugiantoro Bambang Sugiantoro Bayu Triwibowo Bintang Faisal Akbar Budiman, Kholiq Danissa Wirna Karmesti Dyah Rini Indriyanti Efdika, Muhamad Fadil Eling Purwantoyo Elvara Norma Aroyandini Endah Suwarni Enni S. Rahayu Enni Suwarsi Rahayu Fadhila Fauzia Syahriar Fathur Rokhman Fauziyyah, Alyaa Fidia Fibriana Fikri, Irvan Maulana Firman Hardianto Fitarahmawati fitria ningsih Frisca Dynasti Putri Habibah, Berlian Ummu Hadiati, Devi Hadiyanti, Luthfia Nur Hanifah, Husnul Hartono Hartono Hasanah Ratri Handayani, Hasanah Ratri Hendi Pratama Ika Puspita Sari Imam Baihaqi Indriani, Betty Shinta Istianah, Naila Izdiharsant, Almaas Izza, Fika Rofiuddin Juhadi Juhadi Karmesti, Danissa Wirna Kartuti, Kartuti Krispinus Kedati Pukan Laraswati, Vivi Anggraeni Lina Herlina Maghfiroh, Ana Nailatul Maolina Asi Maolina Asi Margareta Rahayuningsih Nugroho Edi K Marini . Mariya Ulfa Mariyam, Siti Neng Maulida Nuradellia Mentari Setiawati, Mentari Moh. Solehatul Mustofa Mualimaturrochmah, Mualimaturrochmah Muamar, Abdullah Mubarak, Albian Mudaningrat, Ajeng Muhammad Abdullah Muhammad Isbat Amrullah Muhammad Khafid Mustofa , Moh. Solehatul Nabila, Chelsea Eka Putrista Narinda Intany, Narinda Ngabiyanto - Nicky, Dhea Ning Setiati Nur Hamid Nur Kusuma Dewi Okky Irmina Safitri, Okky Irmina Oktafiani, Rizka Okta Prikadiastuti, Idaliani Prikadiastuti, Idaliani Priyanti Susanti Putri, Raras Widiasri Rina Munawar Istiani, Rina Munawar Rita Megia Rita Megia Rizka Okta Oktafiani Rizka Oktafiani Rizki, Angela Dita Rohman, Farid Fadhlu Sa'idah, Nailiya Safira Pramudya Wardani Safitri, Naziah Febri Saiful Ridlo Sari, Ika Puspita Sa’adah, Nur Shofiyati, Aida Sigit Saptono Siti Alimah Solichin Solichin Sri Ngabekti Sri Rizki Sianturi, Advend Subiyati Sucihatiningsih Dian Wisika Prajanti SUMADI SUMADI Supriyadi Supriyadi Susanti - Susanti - Susanti, Priyanti Syuru, Awan Usy Talitha Widiatningrum Thalita Widiatiningrum Timotius Surya Saputra Tommi Yuniawan Tri Lestari Handayani Tris Sugiarto Tuti Widianti Tuti Widianti Utis Sutisna Vitradesie Noekent, Vitradesie Wasil Hidayah, Wasil Widiatiningrum, Thalita Widowati, Widowati Wiwi Isnaeni YB. Praharto Yundari, Yundari Yustinus Ulung Anggraito Zahra, Oktavina Nikmatuz Zubail, Dien Rosiana

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