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All Journal Medicina E-Jurnal Medika Udayana JURNAL BIOMEDIK WMJ (Warmadewa Medical Journal) Molecular and Cellular Biomedical Sciences (MCBS) Berkala Ilmiah Kedokteran Duta Wacana ISM (Intisari Sains Medis) : Jurnal Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Lumbung Farmasi : Jurnal Ilmu Kefarmasian IJAAM (Indonesian Journal of Anti-Aging Medicine) Jurnal Sains dan Teknologi Peternakan Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Journal Pharmaceutical Science and Application (JPSA)
I Gusti Made Aman
Department Of Medical Pharmacology, Faculty Of Medicine, Universitas Udayana, Denpasar, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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The oral administration of zinc sulfate in increasing the mass of pancreatic β cell and reducing the level of fasting blood glucose and glycated albumin in male wistar rats (Rattus norvegicus) with diabetes mellitus Silvia Irani; Anak Agung Gede Budhiarta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.82

Abstract

Introduction: Diabetes mellitus (DM) is a degenerative disease that can accelerate the aging process. Zinc is a mineral that has antioxidant potential that can increase the cell growth and it also protects pancreatic β cell mass from cell death during diabetes. This study aimed to prove that oral administration of zinc sulfate could increase the pancreatic β cells mass and decrease the level of fasting blood glucose (FBG) and glycated albumin (GA) in male Wistar rats with DM. Methods: This research employed a pure experimental research with post test only control group design by using 36 male Wistar rats. The placebo group that administered with 1 cc of aquadest and the group of zinc that administered by oral zinc sulfate of 100 mg/kgBW in which had been dissolved with 1 cc of aquadest. The assessment of pancreatic β cell mass and FBG levels and glycated albumin were carried out after 30 days of treatment. Results: The analysis by using the Shapiro-Wilk test was distributed normally. The result of Laven’s test indicated that the distribution of data and variants of the groups were homogeneous. The comparative results of the two groups using an independent t-test, there were significant differences between both groups on the mass of pancreatic β cell and the level of fasting blood glucose and glycated albumin (p <0.05). Conclusion: The oral administration of zinc sulfate can increase the pancreatic β cell mass and decrease the level of FBG and glycated albumin in male Wistar rats with DM.
Administration of oral white oyster mushroom (Pleurotus ostreatus) ethanol extract improved lipid profile as effective as simvastatin in dyslipidemic male wistar rats (Rattus norvegicus) Nadia Anastasia; I Wayan Weta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.93

Abstract

Introduction: Dyslipidemia is an abnormality in lipid metabolism characterized by an increase in total cholesterol, LDL, triglyceride, and decrease HDL. Simvastatin is currently a gold standard drug that has side effects, so an alternative therapy that is relatively safe is needed. The white oyster mushroom extract contains bioactive compounds that have the anti-dyslipidemia effect. The purpose of this study was to prove that the administration of oral white oyster mushroom (Pleurotus ostreatus) extract improves lipid profile as effective as simvastatin in dyslipidemic male Wistar rats (Rattus norvegicus). Methods: This study was an experimental study with pretest-posttest control group design by using 12 rats. Rats were induced by dyslipidemic with a high cholesterol diet for 28 days and divided into 2 groups. The control group was fed with standard food and statins of 10 mg/day and the treatment group was fed with a standard diet and white oyster mushroom extract. Results: The results of paired data analysis showed that both simvastatin and white oyster mushroom extract reduced total cholesterol (p<0,001) and (p<0,001), triglycerides (p<0,001) and (p=0,005), LDL (p<0,001) and (p<0,001) and increased HDL (p <0,001) and (p<0,001). Between groups before treatment (pretest) showed no differences levels in total cholesterol, triglyceride, LDL, and HDL. After treatment (posttest), there were also no differences levels between groups in total cholesterol (p=0,097), triglyceride (p=0,160), LDL (p=0,980), and HDL (p-0,452). Conclusion: The administration of oral white oyster mushroom (Pleurotus ostreatus) extract improved lipid profile as effective as simvastatin in dyslipidemic male Wistar rats (Rattus norvegicus).
Intraperitoneal administration of dexpanthenol inhibits the decrease in the number of leydig and sertoli cells in the wistar strain of white rat (rattus norvegicus) test exposed to monosodium glutamate Susianingsih Murni Hartati; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (674.458 KB)

Abstract

Introduction: Free radical accumulation that goes beyond the ability of the body’s defense mechanism causes oxidative stress which is one of the causes of premature aging in the testicular organs. Dexpanthenol works by increasing the levels of intracellular coenzyme A, ATP and glutathione which play a role in the defense and repair of cells against oxidative and inflammatory stress. The purpose of this study was to prove that intraperitoneal administration of Dexpanthenol inhibited the decrease in the number of Leydig cells and Sertoli cells in the testes of Wistar rats (Rattus norvegicus) exposed to Monosodium glutamate (MSG). Methods: This research is true experimental with post-test only control group design. 32 rats were divided into two groups with each group consisting of 16 rats. The first group was the Control Group (P1) which was given a placebo aquadest twice a week intraperitoneally. The second group was the treatment group (P2) which was given Dexpanthenol 1000 mg / kgBW mice twice a week intraperitoneally. All groups were given 4 g / kgBB of MSG mice every day with sonde. The experiment lasted 14 days and then testicular tissue was taken be evaluated and the number of Leydig cells and Sertolinya cells were counted. Results: The Saphiro-Wilk test results showed that the data of the number of Leydig cells was not normally distributed (p <0.05) while the data of the Sertoli cell counts were normally distributed p> 0.05. Comparative analysis of Leydig cell counts was conducted using Mann-Whitney Test while comparative analysis of Sertoli cell numbers using Independent-T Test. The results of the analysis showed that there were significant differences in the mean number of Leydig cells and Sertoli cells between groups P1 and P2. Conclusion: This study concluded that intraperitoneal administration of Dexpanthenol inhibits the decrease in the number of Leydig cells and Sertoli cells in the testis of Wistar strain white rats (Rattus norvegicus) exposed by Monosodium glutamate.
Implantation of Polydioxonone (PDO) Threads Decreased Dorsolumbar Subcutaneous Fat Weight in Obese Female Rats (Rattus Norvegicus) Diana Niti Santoso; Anak Agung Gde Putra Wiraguna; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.78

Abstract

Introduction: Thread-lifts, especially using polydioxanone (PDO), are thought to have targeted lipolysis effect, but have never been empirically proven. The aim of this study was to prove that the implantation of polydioxonone (PDO) threads decrease dorsolumbar subcutaneous fat weight in obese female rats (Rattus norvegicus). Methods: This experimental study used posttest only control group design. The research subjects were 36 healthy Wistar rats, females, aged 16-18 weeks, obese with a body weight of 250-300 grams (Lee index criteria> 0.3). Obesity was induced by high-carbohydrate and high-fat feed. The rats were then divided into 2 groups, the negative control group with catgut thread implant treatment (P0) and the treatment group with polydioxonone (PDO) thread implant treatment (P1). After 28 days the rats were euthanized, skin tissue was collected, subcutaneous fat was searched and separated from the dorsolumbar depots found in the subcutaneous layer in the lumbar spine at the dorsal part of the body, then measured with the Sartorius brand scales. Results: The median weight of dorsolumbar subcutaneous fat in the P0 group was 1.05 (0.80; 1.90) grams, while in P1 group was 0.90 (0.70; 1.10) grams. The results of the comparative analysis with Mann Whitney test showed a p value of 0.016, which means that there was a difference in dorsolumbar subcutaneous fat between groups after 28 days. Conclusion: Implantation of polydioxonone (PDO) threads decreased dorsolumbar subcutaneous fat weight in obese female rats (Rattus norvegicus).
Oral administration of bitter gourd (Momordica Charantia) extract prevent dyslipidemia on male wistar rats (rattus norvegicus) fed with high-fat diet Hendrik Danico; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.49

Abstract

Introduction: Dyslipidemia is one of the most prevalent health conditions and often leads to development of many chronic diseases such as coronary artery disease, heart failure, and stroke. This study was conducted to investigate the effects of bitter gourd (Momordica Charantia) extract on lipid profiles in male wistar rats fed with high-fat diet. Methods: This study was an experimental research using randomized post-test only control group design. The subjects were 36 male Wistar rats (Rattus norvegicus) randomly divided into 2 groups. The first group was the control group (P0) and fed with placebo while the treatment group (P1) received bitter gourd (Momordica Charantia) ethanol extract at 1g/kg body weight/day. Both groups were fed with high fat diet. The experiment was lasted for 35 days and lipid profiles were measured at the end of the experiment. Results: Statistical analysis results revealed the mean of total cholesterol, triglyceride, and low-density lipoprotein (LDL) in the treatment group were significantly lower than control group while high-density lipoprotein (HDL) was significantly higher in the treatment group (p<0.05). Conclusion: This study concluded that bitter gourd extract significantly prevent dyslipidemia on male Wistar rats fed with high-fat diet.
Macassar fruit extract (Brucea javanica (l.) merr) increased the level of superoxide dismutase (SOD) but had no effect on the level of malondialdehyde (mda) in paraquat-treated male swiss Webster mice Maria Eka Patri Yuliati; I Gusti Made Aman; Ni Nyoman Ayu Dewi
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 2 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.984 KB) | DOI: 10.36675/ijaam.v3i2.41

Abstract

Introduction: Paraquat exposure causes aging because it induces oxidative stress marked by decreased level of SOD and increased MDA serum level. Macassar fruit contains bioactive compounds such as vitamin C, flavonoids, tannins and polyphenol that have antioxidant activity. The purpose of this study was to prove that Macassar fruit extract increased the level of Superoxide Dismutase (SOD) enzyme and reduce the level of Malodialdehid (MDA) in male Swiss Webster mice treated by paraquat. Methods: A randomized pretest-posttest control group study was conducted using 14 male mice which were 2-3 months old, healthy and had 25-30 gram in weight. They divided into 2 groups namely P0 (control) and P1 (Treatment). Both groups were treated by paraquat but only group P1 received 20 mg Macassar fruit extract while the P0 only got 1 cc placebo for 14 days. Results: Our result showed that the level of SOD was increased in P1 group from 17.18±1.69 U/ml to 67.56±3.65 U/ml (p<0.01) while no change was observed in P0 group 16.97 ±1.45U/ml to 17.07±1.89 U/ml (p>0.05). However, no effect on MDA level was observed as the level of MDA tended to slightly decrease in both groups. Conclusion: It can be concluded that Macassar fruit extract 20 mg/kgBB significantly increased the level of SOD whilehave no effect on MDA level in male mice Swiss Webster treated by paraquat.
Ethanol extract of red fruit (Pandanus conoideus lam.) improved lipid profile of dyslipidemic-male wistar rats (Rattus norvegicus) Sriwiyanti Sriwiyanti; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.88

Abstract

Introduction: Dyslipidemia is one of aging- related disease associated with unhealthy food such as high calories and fat. Pandanus Conoideus Lam. with high antioxidant capacity might ameliorates dyslipidemia phenotypes. This study aimed to prove that ethanol extract of red fruit improves lipid profiles of dyslipidemic-rats (Rattus norvegicus). Methods: This was an experimental study with a pretest-posttest control group design, using 14 wistar rats males. Rats were then divided into 2 groups (n= 7). The first group was treated with aquabidestilata 1 mL for 30 days (P0). The second group was treated with extract of red fruit ethanol of 168 mg/200 gr BW for 30 days (P1). Results: The control group (P0) treated with aquabidestilata, total cholesterol levels increased into 221.48 ± 3.64 mg/dl (p <0.05), triglyceride levels elevated into 132.92±3.01 mg/dl (p <0.05), HDL levels depleted into 22.83 ± 2.26 mg/dl (p <0.05), and LDL levels was increased from 76.77 ± 3.33 mg / dl to 78.63 ± 2.84 mg / dl (p <0.05). In contrast, the red fruit ethanol extract in treatment group (P1) experienced decrease on total cholesterol level into 118.41 ± 2.15 mg/dl (p <0.05), triglyceride levels into 91.81 ± 2.71 mg/dl (p <0.05), and LDL levels into 45.85 ± 2.59 mg/dl (p <0.05), and elevation on HDL levels from 25.77 ± 1.36 mg/dl to 55.76 ± 2.35 mg/dl (p <0.05). Conclusion: This study indicated that administration of ethanol extract of red fruit (Pandanus conoideus lam.) improved lipid profiles of dyslipidemic-rats (Rattus norvegicus).
Topical bromelain cream did not increase neovascularization, fibroblast and epithelialization in wound healing of diabetic-rats (rattus norvegicus) Maulidha Suzi Annisa; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.90

Abstract

Introduction: The diabetic-wound healing process is longer due to blood vessel ischemia. Bromelain is the enzyme that acts as debridement agent with anti-inflammatory effect. The aim of this study was to prove the topical administration of bromelain increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats. Methods: This study used post-test only control group design. Subjects were 32 male Wistar diabetic-rats (Rattus norvegicus. It was divided into 2 groups (n = 16) and given amoxiciline for 3 days. The control group consist of: O1 group treated with a base cream for 5 days and O2 group was treated for 12 days. While the treatment group consist of: O3 group was treated with 70% bromelain cream for 5 days and O4 group was treated for 12 days. The neovascularization, fibroblasts, and epithelialization were examined histopathologically. Results: The mean number of fibroblasts in O1 and O3 were 222.88±37.94 cells/field of view and 25.38±7.94 cells/field of view, while O2 and O4 were 99.63±82.33 cells/field of view and 32.25±12.453 cells/field of view (p <0.001). The mean number of neovascularization in O1 and O3 were 15.13±1.95 and 2.88±1.80 cells/field of view, while O2 and O4 were 5.50±3.78 cells/field of view and 1.50±1.06 cells/field of view (p <0.001). The epithelial gap size in the O1 and O2 group were 377.56±573.88 μm and 229.04±647.83 μm (p <0,001). Both O3 and O4 groups showed no longer a wound gap (p>0,05). Conclusion: Topical bromelain cream 70% did not increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats.
Administration of gallus domesticus boiled chicken white egg increased amh levels of premenopausal female wistar rats Liya Rosdiana; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.108 KB)

Abstract

Introduction: Aging is a complex multifactorial process and leads to functional changes one of which is the decline in reproductive function. Decrease in reproductive function in women in in the form of decreased ovarian reserve can be detected by low levels of Anti_mullerian Hormone (AMH). This study aims to prove the effect of administration of Gallus domesticus boiled chicken white egg increased AMH levels of premenopausal female wistar rats. Methods: This research was experimental animal with Pretest-Posttest Control Group Design. The study conducted on 16 premenopausal female wistar mices, aged 17-18 months, weighing 250-260 grams. The mice were divided into 2 goups: the control group (placebo), which was given gelatin flour by 7.3 gram 3 times daily for 4 weeks and the group treted with Gallus domesticus boiled chicken white egg by 7.3 gram 3 times daily for 4 weeks by oral. Blood AMH level were measured before and after treatment for both group. Results: The result showed that before treatment, mean AMH level both in control group and treatment group were not significantly different (p > 0,05). After treatment, mean AMH level both in control group and treatment group were significantly different (p < 0,05). The administration of Gallus domesticus boiled chicken white egg increased AMH levels by 0.804 ng/ml. Conclusion: It can be concluded that administration of Gallus domesticus boiled chicken white egg increased AMH level. The mean AMH level after treatment had a significant difference between control group (placebo) and treatment group with p = 0.001.
Oral Administration of Polypodium leucotomos Powder Inhibited Sunburn Cells Formation in UVB-Induced Male Wistar Rats (Rattus Norvegicus) Priskila Ariana; I Gusti Made Aman; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.59

Abstract

Introduction: UVB can cause direct damage to the DNA and eventually cause apoptosis of epidermal keratinocytes, which is called a sunburn cells. Polypodium leucotomos (Heliocare®) is one of the skin photoprotectors that can modulate immune and inflammatory responses that play a role in preventing sunburn. This study aimed to prove that oral administration of Polypodium leucotomos powder inhibits sunburn cells formation in UVB-induced male Wistar rats (Rattus norvegicus). Methods: This study was a true experimental study using post-test only control group design consisted of two groups with 18 rats/group. One group as a control group (P0) that was treated with oral 1ml aquadest (force feeding) for 10 days. The other group was the treatment group (P1) treated with oral Heliocare® dry powder (Polypodium leucotomos) of 80 mg/kg BW diluted in 1 ml aquadest (force feeding) for 10 days. On the day 11, the dorsal skin was shaved and exposed to single dose UVB of 25mJ. Twenty-four hours later, rats were sacrificed and skin samples were collected for histology preparations with Hematoxylin-Eosin staining and sunburn cells were counted as posttest data. Results: The results showed that the average number of sunburn cells in the P0 group was 36.5±7.53 sunburn cells/mm2, while in the P1 group was 8.3±3.59 sunburn cells/mm2 (p <0.05). Those data showed that there was a significant different of average number of sunburn cells among group tested. Conclusion: It can be concluded that oral administration of 80 mg/kg BW Polypodium leucotomos powder inhibited sunburn cells formation in UVB-induced male Wistar rats (Rattus norvegicus).
Co-Authors ., Nuriyah A.A.G.P. Wiraguna AAGP Wiraguna Agung Nova Mahendra Akpriyanti, DG Ika Ana Yulia Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Arina Papita Simanungkalit Augus Venty Bagiada NA Bagus Komang Satriyasa Bissi Herliane Citra Anissa Ayu Cynthia Sofyanti Sugiharto Delly Dahlia Dewa Ayu Made Adnya Swari Dewa Ayu Made Adnya Swari Dhiancinantyan Windydaca Brata Putri Dian Andriani Dian Andrieany Husodo Diana Niti Santoso Dinda Paramaningtyas Sudibya Eveline Margo Fanny Rustandi Felix Kusmana Ferbian Milas Siswanto Gde Ngurah Idraguna Pinatih Gede Gunawan Mahardika Golda Bernadet Lemewu Hayatul Firi Hayyu Kusumaningtyas Heidy Dwi Nadia Hendrik Danico Heny Widiyowati S I Gusti Ayu Artini I Ketut Suada I Nyoman Adiputra I Putu Eka Kusuma Yasa I Wayan Martadi Santika I Wayan Martadi Santika I Wayan Putu Sutirta Yasa I Wayan Weta Ida Ayu Dewi Wiryanthini Ida Sri Iswari Irtanto, Okky J. A. Pangkahila Ken Andrina Anindita Ketut Widyani Astuti Laura Indriana Liya Rosdiana Made Dio Lokantara Made Oka Negara Manus, Widya Christine Maria Eka Patri Yuliati Maulidha Suzi Annisa Melita Hidajat Nadia Anastasia Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Yuliana Assari Ni Wayan Sucindra Dewi Noviyanti Situmorang Olivia Olivia Priskila Ariana Putu Ayu Melati Widyasari Setia Wardani Silvia Irani Sriwiyanti Sriwiyanti Suarni . Sukoco, Hendro Sulaiman, Yogie Susianingsih Murni Hartati Sutema, Ida Ayu Manik Partha Syafii, Corry P. Valentine Retny Shiajanto Wimpie I Pangkahila Yuliana Tjua