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All Journal Medicina Journal Pharmaceutical Science and Application (JPSA) E-Jurnal Medika Udayana JURNAL BIOMEDIK WMJ (Warmadewa Medical Journal) Molecular and Cellular Biomedical Sciences (MCBS) Berkala Ilmiah Kedokteran Duta Wacana ISM (Intisari Sains Medis) : Jurnal Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Lumbung Farmasi : Jurnal Ilmu Kefarmasian IJAAM (Indonesian Journal of Anti-Aging Medicine) Jurnal Sains dan Teknologi Peternakan Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan

I Gusti Made Aman

Department Of Medical Pharmacology, Faculty Of Medicine, Universitas Udayana, Denpasar, Bali

Author-ID : 3606775

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Oral Administration of Polypodium leucotomos Powder Inhibited Sunburn Cells Formation in UVB-Induced Male Wistar Rats (Rattus Norvegicus) Priskila Ariana; I Gusti Made Aman; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.59

Introduction: UVB can cause direct damage to the DNA and eventually cause apoptosis of epidermal keratinocytes, which is called a sunburn cells. Polypodium leucotomos (Heliocare®) is one of the skin photoprotectors that can modulate immune and inflammatory responses that play a role in preventing sunburn. This study aimed to prove that oral administration of Polypodium leucotomos powder inhibits sunburn cells formation in UVB-induced male Wistar rats (Rattus norvegicus). Methods: This study was a true experimental study using post-test only control group design consisted of two groups with 18 rats/group. One group as a control group (P0) that was treated with oral 1ml aquadest (force feeding) for 10 days. The other group was the treatment group (P1) treated with oral Heliocare® dry powder (Polypodium leucotomos) of 80 mg/kg BW diluted in 1 ml aquadest (force feeding) for 10 days. On the day 11, the dorsal skin was shaved and exposed to single dose UVB of 25mJ. Twenty-four hours later, rats were sacrificed and skin samples were collected for histology preparations with Hematoxylin-Eosin staining and sunburn cells were counted as posttest data. Results: The results showed that the average number of sunburn cells in the P0 group was 36.5±7.53 sunburn cells/mm2, while in the P1 group was 8.3±3.59 sunburn cells/mm2 (p <0.05). Those data showed that there was a significant different of average number of sunburn cells among group tested. Conclusion: It can be concluded that oral administration of 80 mg/kg BW Polypodium leucotomos powder inhibited sunburn cells formation in UVB-induced male Wistar rats (Rattus norvegicus).

Topical application of cinnamon (cinnamomum burmanii) essential oil has the same effectiveness as minoxidil in increasing hair length and diameter size of hair follicles in male white Wistar rats (rattus norvegicus) Laura Indriana; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.501 KB)

Introduction: In aging process, the hair experiences thinning and the hair shaft becomes more fragile due to decreased regeneration of hair. Cinnamon essential oil contains cinnamaldehyde compounds which have a vasodilating effect on blood vessels and stimulating IGF-1 can increase fertility and prevent hair loss. Methods: This study was an experimental study with a post test only control group design carried out in the laboratory animal unit of the pharmacology section of the Udayana University medical faculty. In this study 30 male white rats (Rattus norvegicus) wistar lines were sheared 4cmx4cm which were divided into three groups: negative (aquades) control group (2% minoxidil), treatment group with cinnamon essential oil (concentration 100 %) for 30 days. Results: The average hair length of aquades (P0) was 5.88 ± 0.36 mm, minoxidil group 2% (P1) 7.4 ± 0.20 mm. , cinnamon essential oil group (P2) 8.32 ± 0.18 mm In the diameter size of hair follicles in the three groups showed the mean diameter size of hair follicles was significantly different (P <0.05). The average results of the group hair follicle diameter (P0) were 79.73 ± 30.68 µm, group (P1) 127.07 ± 38.20 µm and group (P2) 130.91 ± 49.96 µm. The LSD test results on hair length measurements between the three groups showed a very significant difference (P <0.01). Group P0 and P1-1,51 mm, group P0 and P2-2,44 mm group P1 and P2 0,92 mm. The results of LSD hair follicle diameter P0 and P1 -47.34 µm (P <0.05), P0 and P2 -51.18 µm (P <0.01), P1 and P2 -3.84 µm (P> 0 , 05) Conclusion: The application of topical cinnamon essential oil increases hair length and diameter size of male wistar rat hair follicles as effectively as topical minoxidil 2%.

Selenium enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs Felix Kusmana; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.79

Abstract Background: Exercise maintains health and prevent aging. However, excessive exercise causes overtraining syndrome. Selenium supplementation may increase cellular antioxidant activity, such as glutathione peroxidase (GPx), thioredoxin reductase (TRx), and selenoprotein P. This study aimed to prove that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs.Methods: This experimental study used randomized pretest-posttest control group design. Subjects were 16 male Wistar rats, 16 weeks old, healthy, weighing 150-160 grams. Subjects were divided randomly into 2 groups (n=8), one group was treated with excessive physical training and 6.48 grams of ordinary eggs, and another group was treated with excessive physical training and 6.48 grams of selenium-enriched eggs for 14 days. Results: Post-test analysis showed that the mean of glutathione peroxidase level in the group given ordinary eggs was 45.90±8.11 mIU/mL, while the group given selenium-enriched eggs was 72.61±7.40 mIU/mL (p= 0.001). In addition, the mean of F2-Isoprostane level in the group given ordinary eggs was 6.37±1.34 ng/ml and the group given selenium-enriched eggs was 3.39±0.77 ng/ml (p= 0,001). Analysis of paired sample t-test revealed that in both groups, glutathione peroxidase levels were increase while F2-isoprostane levels were decrease. Conclusion: This study suggested that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs. Key words: excessive physical training, F2-isoprostane, glutathione peroxidase, selenium-enriched eggs, Wistar rat.

Topical virgin red palm oil improved wound healing by increasing fibroblasts, neovascularization and epithelialization in male Wistar rats (Rattus norvegicus) Heidy Dwi Nadia; I Wayan Weta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.50

Background: The healing process of burns wound can be accelerated using natural compound and one example of alternative medicine is virgin red palm Oil (VRPO). However, scientific evidence that back this notion is still lacking. Therefore, this study aimed to assess the effect VRPO toward burn wound healing in male wistar rats (Rattus norvegicus). Methods: An experimental randomized post-test only control group study was conducted using 28 male Wistar rats. Burn wound was created in all rats but only treatment group received VRPO while the control group was treated with placebo cream. Skin sample was obtained from each rat at day 4 and 11 for histological examination (fibroblasts density, neovascularization, and epithelialization). Results: The results showed that VRPO improved fibroblast density, neovascularization, and epithelialization at day 4 compared to control group. However, at day 11, there was a decreased in fibroblast density and neovascularization while epithelialization continuously increased at double the rate of the control group. Conclusion: Topical VRPO improved burn wound healing by increasing wound healing speed and epithelialization.

Oral administration of clabet (Trigonella foenum graecum) seeds ethanol extracts improved testosteron level in old-male wistar (Rattus norvegicus) mice Bissi Herliane; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.464 KB)

Introduction: Aging can cause decreased testosterone levels in men which is known as andropause. Some herbs are known to increase the levels Testosterone. One of them is Klabet seed that contain phyto-testosterone. The purpose of this study was to prove that the administration of ethanol extract of Klabet seeds increased testosterone levels in old male Wistar rats. Methods: A pretest-posttest control group design study was conducted using 20 male white wistar rats that were divided into 2 (two) groups: control group that received 2 cc of distilled water and treatment group that received 2 cc ethanol extract of Klabet seeds 250mg/250gBB rats/day by sonde for 14 days. The testosterone level was measured on day 1 and 15. Results: The analysis showed that there was no significant difference in the pre-test and post-test testosterone level P0 (Pre-test:16.4±0.553 ng/ml; posttest: 15.9±1.51ng/ml) while increased level of testosterone could be observed in treatment group (P1) (pre-test: 16.6±0.476ng/ml; post-test: 19.4±0.828ng/ml. Comparative analysis between post-test results (P0 vs P1) showed that the difference was statistically significant (p <0.001). Conclusion: Oral administration of Klabet seeds ethanol extract can significantly increase testosterone level in old male wistar mice.

Pomegranate extract (Punica granatum) not as effective as simvastatin in improving profile lipid in dyslipidemic male wistar rats (Rattus norvegicus) Ken Andrina Anindita; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.89

Introduction: Dyslipidemia is one of the factors of the occurrence of heart disease and atherosclerosis. Simvastatin and Pomegranate (Punica granatum) work by improving the lipid profile. The aim of this study was to prove that red pomegranate extract (Punica granatum) improve lipid profile of male white rats that are dyslipidemic. Method: This research is a experimental study with randomized pre test and post test control group design, using 20 adult male white rats as sample. After 30 days, rats were divided into 2 study groups with 10 rats per group. The group was classified into two (P1 and P2). Prior to the treatment, the rats were examined the first diluted T-cholesterol, triglyceride, LDL and HDL levels as pretest data. Treatment was performed for 15 days. Results: The mean pretest HDL level in the simvastatin group was 23.84 ± 2.54 mg / dL and the red pomegranate extract group was 25.41 ± 2.34 mg / dL and p = 0.167. After treatment for 15 days, the mean posttest the red pomegranate extract group was 126.55 ± 3.09 mg / dL and p = 0,001. The mean posttest HDL level in the simvastatin group was 58.72 ± 5.21 mg / dL and the red pomegranate extract group was 40.56 ± 2.87 mg / dL and p = 0,001. Conclusion: The administration of red Punica granatum is not as effective as simvastatin in improving lipid profile in dyslipidemic rats. Although not as effective as simvastatin, red pomegranate extract can significantly improve lipid profile.

The administration of supplements milk with high protein of whey increased estrogen level but did not affect testosterone level in male wistar rat (Rattus norvegicus) Dian Andriani; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.097 KB)

Introduction: Supplements milk is a high protein of whey that consumed by many teenagers to maintain the fitness in accordance with the advertisements in the society, the presence of hormones in milk that are not listed in its composition causes a hormonal balance disorder. The aim of this research was to prove that giving supplements milk with a high protein of whey in increasing estrogen levels and lowers testosterone counts in male Wistar rats (Rattus norvegicus). Methods: This research employed experimental research using a pretest-posttest control group design that used 22 white male Wistar rats (Rattus norvegicus), the samples were divided into 2 groups, namely the control group (P0) and the treatment group (P1) that treated by supplements milk with a high-protein of whey once a day for three weeks. The blood was taken before and after treatment for estrogen levels and testosterone counts. Result: The finding indicated that a significant increase in estrogen levels before and after the administration of milk supplement with high whey protein 11.603 ± 0.502 ng/ ml to 16.141 ± 0.347 ng/ ml (p<0.001). There was no decrease in testosterone counts before and after subjects were administered by milk supplement with high whey protein 1.86 ± 0.325 ng/ ml to 1.69 ± 0.260ng/ ml p = 0.220 (p> 0.05). Conclusion: Based on the results of the analysis it could be concluded that the administration of milk supplement with high whey protein could increase estrogen levels while decreasing testosterone in male Wistar rats (Rattus norvegicus).

Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia Melita Hidajat; I Gusti Made Aman; Hendro Sukoco; Ferbian Milas Siswanto
Jurnal Sains dan Teknologi Peternakan Vol 1 No 1 (2019): Jurnal Sains dan Teknologi Peternakan
Publisher : Universitas Sulawesi Barat

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

Soymilk Formula Increases Estrogen and Reduces Testosterone Level in Male Infant White Wistar Rats Eveline Margo; Wimpie Pangkahila; I Gusti Made Aman
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Background: Children’s immunity system is relatively lower on first year of life, causing atopic babies, leading to allergy. Ideally, newborn babies are given breast milk as main food source on the first 6 months, but often being replaced with cow milk which can induce Cow’s Milk Protein Allergy. For the alternative can replace it with soymilk formula which contains phytoestrogen from isoflavon, which works just like estrogen. The aim of this study to determine whether administration of soymilk formula is capable on increasing estrogen level and reducing testosterone level on male infant white Wistar rats (Rattus norvegicus).Materials and Methods: This research used post test only control group design. Samples were consisted of 36 infant male white Wistar rats aged 7 days, weighing from 10-15 grams, divided into 2 groups, control group which was given cow’s milk and intervention group which was given soymilk. Each group were given intervention with the dose 5% of BW (grams), given twice-a-day orally for 21 days, and subsequently in the morning their blood samples were taken to examine the level of estrogen and testosterone hormone.Results: The study showed that the level of estrogen on intervention group was significantly higher than the control group with mean estrogen level of 0.55±0.03 pg/mL in the control group and 0.82±0.01 pg/mL in the intervention group, while the mean testosterone level was significantly lower on intervention group compared with control group (p<0.05) with 3.25±0.15 ng/mL on control group and 2.36±0.22 ng/mL on intervention group.Conclusion: Soymilk was able to increase estrogen level and reduce testosterone level on male infant white Wistar rats (Rattus norvegicus).Keywords: soymilk formula, estrogen, testosterone, male infant rats

IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS) Widya Christine Manus; Wimpie Pangkahila; I Gusti Made Aman
Berkala Ilmiah Kedokteran Duta Wacana Vol 4, No 2 (2019): BERKALA ILMIAH KEDOKTERAN DUTA WACANA
Publisher : Faculty of Medicine Universitas Kristen Duta Wacana

Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure. Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat. Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment. Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/L) compared to the control group (3.091.33x103/L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/L) compared to the control group (6.70 ± 1.96x103/L), with p

Co-Authors ., Nuriyah A.A.G.P. Wiraguna AAGP Wiraguna Agung Nova Mahendra Akpriyanti, DG Ika Ana Yulia Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Arina Papita Simanungkalit Augus Venty Bagiada NA Bagus Komang Satriyasa Bissi Herliane Citra Anissa Ayu Cynthia Sofyanti Sugiharto Delly Dahlia Dewa Ayu Made Adnya Swari Dian Andriani Dian Andrieany Husodo Diana Niti Santoso Dinda Paramaningtyas Sudibya Eveline Margo Fanny Rustandi Felix Kusmana Ferbian Milas Siswanto Gde Ngurah Idraguna Pinatih Gede Gunawan Mahardika Golda Bernadet Lemewu Hayatul Firi Hayyu Kusumaningtyas Heidy Dwi Nadia Hendrik Danico Heny Widiyowati S I Gusti Ayu Artini I Ketut Suada I Nyoman Adiputra I Putu Eka Kusuma Yasa I Wayan Martadi Santika I Wayan Putu Sutirta Yasa I Wayan Weta Ida Ayu Dewi Wiryanthini Ida Sri Iswari Irtanto, Okky J. A. Pangkahila Ken Andrina Anindita Ketut Widyani Astuti Laura Indriana Liya Rosdiana Made Dio Lokantara Made Oka Negara Manus, Widya Christine Maria Eka Patri Yuliati Maulidha Suzi Annisa Melita Hidajat Nadia Anastasia Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Yuliana Assari Ni Wayan Sucindra Dewi Noviyanti Situmorang Olivia Olivia Partha Sutema, Ida Ayu Manik Priskila Ariana Putri, Dhiancinantyan Windydaca Brata Putu Ayu Melati Widyasari Setia Wardani Silvia Irani Sriwiyanti Sriwiyanti Suarni . Sukoco, Hendro Sulaiman, Yogie Susianingsih Murni Hartati Syafii, Corry P. Valentine Retny Shiajanto Wimpie I Pangkahila Yuliana Tjua

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