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All Journal Medicina E-Jurnal Medika Udayana JURNAL BIOMEDIK WMJ (Warmadewa Medical Journal) Molecular and Cellular Biomedical Sciences (MCBS) Berkala Ilmiah Kedokteran Duta Wacana ISM (Intisari Sains Medis) : Jurnal Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Lumbung Farmasi : Jurnal Ilmu Kefarmasian IJAAM (Indonesian Journal of Anti-Aging Medicine) Jurnal Sains dan Teknologi Peternakan Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Journal Pharmaceutical Science and Application (JPSA)

I Gusti Made Aman

Department Of Medical Pharmacology, Faculty Of Medicine, Universitas Udayana, Denpasar, Bali

Author-ID : 3606775

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Selenium enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs Felix Kusmana; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.79

Abstract Background: Exercise maintains health and prevent aging. However, excessive exercise causes overtraining syndrome. Selenium supplementation may increase cellular antioxidant activity, such as glutathione peroxidase (GPx), thioredoxin reductase (TRx), and selenoprotein P. This study aimed to prove that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs.Methods: This experimental study used randomized pretest-posttest control group design. Subjects were 16 male Wistar rats, 16 weeks old, healthy, weighing 150-160 grams. Subjects were divided randomly into 2 groups (n=8), one group was treated with excessive physical training and 6.48 grams of ordinary eggs, and another group was treated with excessive physical training and 6.48 grams of selenium-enriched eggs for 14 days. Results: Post-test analysis showed that the mean of glutathione peroxidase level in the group given ordinary eggs was 45.90±8.11 mIU/mL, while the group given selenium-enriched eggs was 72.61±7.40 mIU/mL (p= 0.001). In addition, the mean of F2-Isoprostane level in the group given ordinary eggs was 6.37±1.34 ng/ml and the group given selenium-enriched eggs was 3.39±0.77 ng/ml (p= 0,001). Analysis of paired sample t-test revealed that in both groups, glutathione peroxidase levels were increase while F2-isoprostane levels were decrease. Conclusion: This study suggested that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs. Key words: excessive physical training, F2-isoprostane, glutathione peroxidase, selenium-enriched eggs, Wistar rat.

Topical virgin red palm oil improved wound healing by increasing fibroblasts, neovascularization and epithelialization in male Wistar rats (Rattus norvegicus) Heidy Dwi Nadia; I Wayan Weta; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 1 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i1.50

Background: The healing process of burns wound can be accelerated using natural compound and one example of alternative medicine is virgin red palm Oil (VRPO). However, scientific evidence that back this notion is still lacking. Therefore, this study aimed to assess the effect VRPO toward burn wound healing in male wistar rats (Rattus norvegicus). Methods: An experimental randomized post-test only control group study was conducted using 28 male Wistar rats. Burn wound was created in all rats but only treatment group received VRPO while the control group was treated with placebo cream. Skin sample was obtained from each rat at day 4 and 11 for histological examination (fibroblasts density, neovascularization, and epithelialization). Results: The results showed that VRPO improved fibroblast density, neovascularization, and epithelialization at day 4 compared to control group. However, at day 11, there was a decreased in fibroblast density and neovascularization while epithelialization continuously increased at double the rate of the control group. Conclusion: Topical VRPO improved burn wound healing by increasing wound healing speed and epithelialization.

Oral administration of clabet (Trigonella foenum graecum) seeds ethanol extracts improved testosteron level in old-male wistar (Rattus norvegicus) mice Bissi Herliane; Wimpie Pangkahila; IGM Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 2 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.464 KB)

Introduction: Aging can cause decreased testosterone levels in men which is known as andropause. Some herbs are known to increase the levels Testosterone. One of them is Klabet seed that contain phyto-testosterone. The purpose of this study was to prove that the administration of ethanol extract of Klabet seeds increased testosterone levels in old male Wistar rats. Methods: A pretest-posttest control group design study was conducted using 20 male white wistar rats that were divided into 2 (two) groups: control group that received 2 cc of distilled water and treatment group that received 2 cc ethanol extract of Klabet seeds 250mg/250gBB rats/day by sonde for 14 days. The testosterone level was measured on day 1 and 15. Results: The analysis showed that there was no significant difference in the pre-test and post-test testosterone level P0 (Pre-test:16.4±0.553 ng/ml; posttest: 15.9±1.51ng/ml) while increased level of testosterone could be observed in treatment group (P1) (pre-test: 16.6±0.476ng/ml; post-test: 19.4±0.828ng/ml. Comparative analysis between post-test results (P0 vs P1) showed that the difference was statistically significant (p <0.001). Conclusion: Oral administration of Klabet seeds ethanol extract can significantly increase testosterone level in old male wistar mice.

Pomegranate extract (Punica granatum) not as effective as simvastatin in improving profile lipid in dyslipidemic male wistar rats (Rattus norvegicus) Ken Andrina Anindita; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.89

Introduction: Dyslipidemia is one of the factors of the occurrence of heart disease and atherosclerosis. Simvastatin and Pomegranate (Punica granatum) work by improving the lipid profile. The aim of this study was to prove that red pomegranate extract (Punica granatum) improve lipid profile of male white rats that are dyslipidemic. Method: This research is a experimental study with randomized pre test and post test control group design, using 20 adult male white rats as sample. After 30 days, rats were divided into 2 study groups with 10 rats per group. The group was classified into two (P1 and P2). Prior to the treatment, the rats were examined the first diluted T-cholesterol, triglyceride, LDL and HDL levels as pretest data. Treatment was performed for 15 days. Results: The mean pretest HDL level in the simvastatin group was 23.84 ± 2.54 mg / dL and the red pomegranate extract group was 25.41 ± 2.34 mg / dL and p = 0.167. After treatment for 15 days, the mean posttest the red pomegranate extract group was 126.55 ± 3.09 mg / dL and p = 0,001. The mean posttest HDL level in the simvastatin group was 58.72 ± 5.21 mg / dL and the red pomegranate extract group was 40.56 ± 2.87 mg / dL and p = 0,001. Conclusion: The administration of red Punica granatum is not as effective as simvastatin in improving lipid profile in dyslipidemic rats. Although not as effective as simvastatin, red pomegranate extract can significantly improve lipid profile.

The administration of supplements milk with high protein of whey increased estrogen level but did not affect testosterone level in male wistar rat (Rattus norvegicus) Dian Andriani; Wimpie Pangkahila; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 3 No 1 (2019): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.097 KB)

Introduction: Supplements milk is a high protein of whey that consumed by many teenagers to maintain the fitness in accordance with the advertisements in the society, the presence of hormones in milk that are not listed in its composition causes a hormonal balance disorder. The aim of this research was to prove that giving supplements milk with a high protein of whey in increasing estrogen levels and lowers testosterone counts in male Wistar rats (Rattus norvegicus). Methods: This research employed experimental research using a pretest-posttest control group design that used 22 white male Wistar rats (Rattus norvegicus), the samples were divided into 2 groups, namely the control group (P0) and the treatment group (P1) that treated by supplements milk with a high-protein of whey once a day for three weeks. The blood was taken before and after treatment for estrogen levels and testosterone counts. Result: The finding indicated that a significant increase in estrogen levels before and after the administration of milk supplement with high whey protein 11.603 ± 0.502 ng/ ml to 16.141 ± 0.347 ng/ ml (p<0.001). There was no decrease in testosterone counts before and after subjects were administered by milk supplement with high whey protein 1.86 ± 0.325 ng/ ml to 1.69 ± 0.260ng/ ml p = 0.220 (p> 0.05). Conclusion: Based on the results of the analysis it could be concluded that the administration of milk supplement with high whey protein could increase estrogen levels while decreasing testosterone in male Wistar rats (Rattus norvegicus).

Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia Melita Hidajat; I Gusti Made Aman; Hendro Sukoco; Ferbian Milas Siswanto
Jurnal Sains dan Teknologi Peternakan Vol 1 No 1 (2019): Jurnal Sains dan Teknologi Peternakan
Publisher : Universitas Sulawesi Barat

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

Soymilk Formula Increases Estrogen and Reduces Testosterone Level in Male Infant White Wistar Rats Eveline Margo; Wimpie Pangkahila; I Gusti Made Aman
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Background: Children’s immunity system is relatively lower on first year of life, causing atopic babies, leading to allergy. Ideally, newborn babies are given breast milk as main food source on the first 6 months, but often being replaced with cow milk which can induce Cow’s Milk Protein Allergy. For the alternative can replace it with soymilk formula which contains phytoestrogen from isoflavon, which works just like estrogen. The aim of this study to determine whether administration of soymilk formula is capable on increasing estrogen level and reducing testosterone level on male infant white Wistar rats (Rattus norvegicus).Materials and Methods: This research used post test only control group design. Samples were consisted of 36 infant male white Wistar rats aged 7 days, weighing from 10-15 grams, divided into 2 groups, control group which was given cow’s milk and intervention group which was given soymilk. Each group were given intervention with the dose 5% of BW (grams), given twice-a-day orally for 21 days, and subsequently in the morning their blood samples were taken to examine the level of estrogen and testosterone hormone.Results: The study showed that the level of estrogen on intervention group was significantly higher than the control group with mean estrogen level of 0.55±0.03 pg/mL in the control group and 0.82±0.01 pg/mL in the intervention group, while the mean testosterone level was significantly lower on intervention group compared with control group (p<0.05) with 3.25±0.15 ng/mL on control group and 2.36±0.22 ng/mL on intervention group.Conclusion: Soymilk was able to increase estrogen level and reduce testosterone level on male infant white Wistar rats (Rattus norvegicus).Keywords: soymilk formula, estrogen, testosterone, male infant rats

IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS) Widya Christine Manus; Wimpie Pangkahila; I Gusti Made Aman
Berkala Ilmiah Kedokteran Duta Wacana Vol 4, No 2 (2019): BERKALA ILMIAH KEDOKTERAN DUTA WACANA
Publisher : Faculty of Medicine Universitas Kristen Duta Wacana

Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure. Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat. Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment. Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/L) compared to the control group (3.091.33x103/L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/L) compared to the control group (6.70 ± 1.96x103/L), with p

Gambaran Pengetahuan, Sikap dan Praktek Masyarakat Terhadap Protokol Kesehatan dalam Pencegahan dan Pengendalian COVID-19 di Kecamatan Denpasar Barat Made Dio Lokantara; Dhiancinantyan Windydaca Brata Putri; I Gusti Made Aman
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 3, No 2 (2022): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v3i2.7701

ABSTRAKProtokol kesehatan merupakan bentuk pencegahan COVID-19 yang berkaitan dengan perilaku masyarakat sekaligus menjadi faktor utama keberhasilan dalam memutus rantai penularan COVID-19. Kecamatan Denpasar Barat merupakan kecamatan di Kota Denpasar dengan angka kejadian kasus tertinggi COVID-19, dimana tingginya angka kejadian kasus ini tidak lepas dari perilaku masyarakat dalam melaksanakan protokol kesehatan di tempat dan fasilitas umum. Tujuan dari penelitian ini adalah untuk mengetahui gambaran pengetahuan, sikap dan praktek masyarakat terhadap protokol kesehatan dalam pencegahan dan pengendalian COVID-19. Metode penelitian ini adalah deskriprif dengan pendekatan cross sectional. Sampel yang digunakan adalah masyarakat Kecamatan Denpasar Barat yang memenuhui kriteria inklusi sebanyak 110 responden. Hasil penelitian menunjukkan responden yang memiliki pengetahuan baik berjumlah 81 responden (73,6%), kategori cukup sebesar 19 responden (17,3%) dan kategori kurang sebesar 10 responden (9,1%). Responden yang memiliki sikap positif berjumlah 60 responden (54,6%) dan memiliki sikap negatif sebanyak 50 responden (45,4%). 68 responden (61,8%) masuk kedalam kategori praktek yang baik, kategori cukup baik sebesar 9 responden (8,2%) dan kategori kurang baik sebesar 33 responden (30,0%) terhadap protokol kesehatan. Simpulan dari penelitian ini adalah masyarakat Kecamatan Denpasar Barat memiliki pengetahuan yang baik, sikap yang positif, dan praktek yang baik terhadap terhadap protokol kesehatan dalam pencegahan dan pengendalian COVID-19. Kata kunci : Pengetahuan; Sikap; Praktek; Protokol Kesehatan; Covid-19.ABSTRACTThe health protocol is a form of preventing COVID-19 related to community behavior as well as being the main success factor in breaking the chain of transmission of COVID-19. West Denpasar Sub-District is a sub-district in Denpasar City with the highest cases of COVID-19, wherever the highest cases cannot be separated from the behavior of the community in implementing health protocols. The purpose of this study was to describe the knowledge, attitudes and practices of the community towards health protocols in the prevention and control of COVID-19. The method used in this research was descriptive with a cross sectional approach. The sample used was the people of West Denpasar Sub-District who met the inclusion criteria of 110 respondents. The results showed that respondents who had good knowledge were 81 respondents (73.6%), sufficient category was 19 respondents (17.3%) and knowledge category was less than 10 respondents (9.1%). Respondents who have a positive attitude were 60 respondents (54.6%) and a negative attitude were 50 respondents (45.4%). 68 respondents (61.8%) fall into the good practice category, good enough category were 9 respondents (8.2%) and bad practice category were 33 respondents (30.0%) regarding health protocols. Based on the research, it can be concluded that the people of West Denpasar Sub-District have good knowledge, positive attitudes, and good practices towards health protocols in the prevention and control of COVID-19. Keywords : Knowledge; Attitude; Practice; Health Protocol; Covid-19.

Pemberian deer placenta secara oral meningkatkan kadar hormon testosteron pada tikus Wistar (Rattus norvegicus) jantan muda I Made Oka Negara; Wimpie Pangkahila; I Gusti Made Aman; Alex Pangkahila; I Wayan Weta; Ni Nyoman Ayu Dewi
Intisari Sains Medis Vol. 11 No. 2 (2020): (Available online: 1 August 2020)
Publisher : DiscoverSys Inc.

Background: Men who experience hypogonadal symptoms are increasingly experienced by a younger age. This is due to a decrease in the hormone testosterone. Efforts to increase the hormone testosterone in hypogonadal men are often made to restore sexual function, libido, muscle mass, physical strength, bone density, and feelings of pleasure. This study aims to determine the effect of giving deer placenta orally in increasing levels of the hormone testosterone in young male Wistar rats (Rattus norvegicus).Methods: The experimental study was conducted using a pretest and posttest control group design involving 18 young male Wistar rats aged 2.5-3 months with a bodyweight of 150-180 grams. Wistar rats were divided into two groups, each totaling 9 rats, one group as a control group (giving placebo for 28 days) and the treatment group (giving deer placenta orally as much as 10.5 grams per day for 28 days). The data obtained were analyzed by SPSS version 20 for Windows.Results: The results showed that there was a significant increase in testosterone levels both before (3.018Â±0.282 ng/ml) and after (4.516Â±0.796 ng/ml) giving deer placenta orally as much as 10.5 grams for 28 days (p <0.010). However, the results of this study showed that there was no increase in testosterone levels in the control group either before or after the placebo treatment (p> 0.05).Conclusion: The results of this study indicate that the administration of deer placenta orally for 28 days showed an increase in testosterone levels among young male Wistar rats.Â Latar Belakang: Laki-laki yang mengalami gejala-gejala hipogonad semakin dialami oleh usia yang semakin muda. Hal ini dikarenakan terjadi penurunan hormone testosterone. Upaya meningkatkan hormon testosteron pada laki-laki hipogonad kerap dilakukan untuk dapat mengembalikan fungsi seksual, libido, masa otot, kekuatan fisik, densitas tulang, dan perasaan senang. Penelitian ini bertujuan untuk mengetahui efek pemberian deer placenta secara oral dalam meningkatkan kadar hormon testosteron pada tikus Wistar (Rattus norvegicus) jantan muda.Metode: Penelitian eksperimental dilakukan dengan menggunakan pretest and posttest control group design yang melibatkan 18 ekor tikus putih Wistar jantan muda berusia 2,5-3 bulan dengan berat badan 150-180 gram. Tikus Wistar terbagi menjadi dua kelompok masing-masing berjumlah 9 ekor tikus dimana satu kelompok sebagai kelompok kontrol (pemberian plasebo selama 28 hari) dan kelompok perlakuan (pemberian deer placenta secara oral sebanyak 10,5 gram per hari selama 28 hari).Â Data yang diperoleh dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Hasil penelitian menunjukkan terdapat peningkatan kadar testostesteron secara bermakna baik sebelum (3,018Â±0,282 ng/ml) maupun setelah (4,516Â±0,796 ng/ml) pemberian deer placenta secara oral sebanyak 10,5 gram selama 28 hari (p<0,010). Akan tetapi hasil penelitian ini menunjukkan tidak terdapat peningkatan kadar testosteron pada kelompok kontrol baik sebelum atau setelah perlakuan yang diberikan plasebo (p>0,05).Kesimpulan: Hasil penelitian ini menunjukkan bahwa pemberian deer placenta secara oral selama 28 hari menunjukkan terdapat peningkatan kadar testosteron pada tikus Wistar jantan muda.

Co-Authors ., Nuriyah A.A.G.P. Wiraguna AAGP Wiraguna Agung Nova Mahendra Akpriyanti, DG Ika Ana Yulia Anak Agung Gde Putra Wiraguna Anak Agung Gede Budhiarta Arina Papita Simanungkalit Augus Venty Bagiada NA Bagus Komang Satriyasa Bissi Herliane Citra Anissa Ayu Cynthia Sofyanti Sugiharto Delly Dahlia Dewa Ayu Made Adnya Swari Dewa Ayu Made Adnya Swari Dhiancinantyan Windydaca Brata Putri Dian Andriani Dian Andrieany Husodo Diana Niti Santoso Dinda Paramaningtyas Sudibya Eveline Margo Fanny Rustandi Felix Kusmana Ferbian Milas Siswanto Gde Ngurah Idraguna Pinatih Gede Gunawan Mahardika Golda Bernadet Lemewu Hayatul Firi Hayyu Kusumaningtyas Heidy Dwi Nadia Hendrik Danico Heny Widiyowati S I Gusti Ayu Artini I Ketut Suada I Nyoman Adiputra I Putu Eka Kusuma Yasa I Wayan Martadi Santika I Wayan Martadi Santika I Wayan Putu Sutirta Yasa I Wayan Weta Ida Ayu Dewi Wiryanthini Ida Sri Iswari Irtanto, Okky J. A. Pangkahila Ken Andrina Anindita Ketut Widyani Astuti Laura Indriana Liya Rosdiana Made Dio Lokantara Made Oka Negara Manus, Widya Christine Maria Eka Patri Yuliati Maulidha Suzi Annisa Melita Hidajat Nadia Anastasia Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Nyoman Ayu Dewi Ni Putu Yuliana Assari Ni Wayan Sucindra Dewi Noviyanti Situmorang Olivia Olivia Priskila Ariana Putu Ayu Melati Widyasari Setia Wardani Silvia Irani Sriwiyanti Sriwiyanti Suarni . Sukoco, Hendro Sulaiman, Yogie Susianingsih Murni Hartati Sutema, Ida Ayu Manik Partha Syafii, Corry P. Valentine Retny Shiajanto Wimpie I Pangkahila Yuliana Tjua

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