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All Journal Buletin Peternakan AGRIMOR JAS (Journal of Animal Science) Pro-Life Animal Production : Indonesian Journal of Animal Production JURNAL ILMIAH PETERNAKAN TERPADU Jurdimas (Jurnal Pengabdian Kepada Masyarakat) Royal Bakti Cendana: Jurnal Pengabdian Masyarakat Journal of Tropical Animal Science and Technology Jurnal Pengabdian UNDIKMA Livestock and Animal Research JAS (Journal of Animal Science)
Lisnahan, Charles V.
Timor University
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Journal : Pro-Life

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Isolasi dan Uji Patogen Bakteri Asam Laktat (BAL) dari Usus Ayam Kampung yang Dipelihara Secara Intensif Di Kefamenanu Welsiliana; Charles Venirius Lisnahan; Lukas Pardosi
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aimed to isolate LAB from the intestines of native chickens and to test the ability of LAB activity against pathogenic bacteria. Bacterial isolation was carried out by taking chicken intestines, then diluting up to 102. The results of the dilution were taken 1 ml to be inoculated into MRSA media using the pour plate technique, streak plate technique was used to obtain pure cultures and then incubated at 37˚C for 24 hours. LAB culture testing for pathogenic bacteria was carried out using MHA media containing Escherichia coli cultures, then incubation for 120 hours at 37˚C. Observation parameters included colony shape, colony color, number of LAB colonies, the diameter of the clear zone and LAB colonies. The results showed that the bacteria isolated from the intestines of native chickens were LAB which had around colony shape and were yellowish-white (beige)-milky white. The number of LAB colonies obtained was 3.7 x 105 CFU/ml with the best inhibitory ability of pathogenic bacteria found in USA 3 isolates (1.85 cm clear zone; 0.90 cm colony size) after incubation for 120 hours.
Co-Authors Agustina Foni Agustina Viktoria Tae Agustinus A. Dethan Agustinus Agung Dethan Alfonsius Bria Alfonsus Seran Alfred Nubatonis Angela Lake Arta S. Kasse Aurelia Abi Azor Yulianus Tefa Baso, Budiman Bira, Gerson Frans Carla Elisabeth De Araujo Deddyano Kandido Son Dira Asri Pramita Elu, Clarissa Dorince Elu, Maria Eriska Gerson F Bira Gerson F. Bira Gerson Frans Bira Irna Oktoviana Selan Lukas Pardosi Manalu, Adelya I Maria F. E. Wamur Martha Ludigadris Amleni Metriana Lalisuk Modesta Salu Nahak, Oktavianus Rafael Nahak, Oktovianus Rafael Natalius Abi Nubatonis, Agustinus Oktovianus R. Nahak Oktovianus R. Nahak Oktovianus R. Nahak, T.B Oktovianus Rafael Nahak Oktovianus Rafael Nahak Oktovianus Rafael Nahak Ori Onesimus Maunaben Pasi, Maria Selfiana Polikarpia Wilhelmina Bani Purwantiningsih, Theresia I. Regina Funan Risald Selviana Suni Serfina Kolo Sri Harimurti Sri Harimurti Stefanus Kolo Theresia I. Purwantiningsih Theresia Ika Purwatiningsih Uduk, Mersiana Ullu, Hevi Herlina Umbu Joka Viktor Fernandes Fahik Welsiliana Wihandoyo Wihandoyo, Wihandoyo Wihandoyo, Wihandoyo Wilda Lumban Tobing Yovita Florida Seran Yuliana Mau Zuprizal (Zuprizal)