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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Bioscience Microbiology Indonesia Jurnal Pendidikan Sains AL KAUNIYAH Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Quagga Gontor AGROTECH Science Journal Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Biodjati Jurnal Pengabdian Pada Masyarakat JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi KACANEGARA Jurnal Pengabdian pada Masyarakat AL-HAYAT: Journal of Biology and Applied Biology Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Kontribusia : Research Dissemination for Community Development International Journal of Engineering, Technology and Natural Sciences (IJETS) Jurnal Berdaya Mandiri Jurnal Sains dan Edukasi Sains Indonesian Journal of Applied Research (IJAR) Indobiosains Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Masyarakat Indonesia International Journal of Research in Community Services Aptekmas : Jurnal Pengabdian Kepada Masyarakat International Journal on Education Insight Journal of Biotechnology and Natural Science Filogeni: Jurnal Mahasiswa Biologi Jurnal Pengabdian Masyarakat Bangsa DIMASLIA Jurnal Abdimas
Oktira Roka Aji
Program Studi Biologi Fakultas Sains Dan Teknologi Terapan Universitas Ahmad Dahlan
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Journal : AL KAUNIYAH

 1 
Bakteri Endofit Tanaman Jeruk Nipis (Citrus aurantifolia) Penghasil Asam Indol Asetat (AIA) Aji, Oktira Roka; Lestari, Iva Dita
Al-Kauniyah: Jurnal Biologi Vol 13, No 2 (2020): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v13i2.13044

Abstract

AbstrakBakteri endofit hidup dalam suatu tanaman tanpa menyebabkan gangguan bagi tanaman yang berperan penting dalam menstimulasi pertumbuhan tanaman, yaitu dengan memproduksi fitohormon seperti asam absisat, asam indol asetat, dan sitokinin. Penelitian ini bertujuan untuk mengisolasi, menyeleksi, dan mengidentifikasi bakteri endofit yang terdapat pada daun, batang, dan akar tanaman jeruk nipis (Citrus aurantifolia). Isolat bakteri endofit diseleksi berdasarkan kemampuannya dalam menghasilkan asam indol asetat (AIA). Isolat bakteri endofit ditumbuhkan pada media nutrient broth (NB) yang ditambah dengan L-triptofan. Konsentrasi AIA dihitung dengan penambahan reagen salkowski dan diukur menggunakan spektrofotometer pada panjang gelombang 530 nm. Identifikasi bakteri endofit dilakukan dengan analisis uji biokimia. Isolat bakteri endofit yang berhasil diisolasi sebanyak 12 isolat, yaitu 4 isolat dari daun, 4 isolat dari batang, dan 4 isolat dari akar. Hasil pengamatan pada uji AIA menunjukkan bahwa semua isolat bakteri endofit dapat menghasilkan hormon AIA. Isolat yang menghasilkan konsentrasi hormon AIA tertinggi adalah isolat B2 (6,51 ppm). Isolat bakteri yang berhasil diidentifikasi berasal dari genus Enterobacter, Bacillus, Pseudomonas, dan Staphylococcus. Bakteri endofit yang dapat menghasilkan AIA berpotensi dikembangkan sebagai biofertilizer untuk meningkatkan produktivitas tanaman. Abstract Endophytic bacteria live inside plants without causing disruption to plants and play an important role in stimulating plant growth. This study aims to isolate endophytic bacteria from lime plant (Citrus aurantifolia) and characterize its ability to produce indole acetic acid (IAA). Bacterial isolates were grown on media supplemented with L-tryptophan as IAA precursor. The bacterial supernatant was mixed with salkowski reagents and then measured using a spectrophotometer at 530 nm. Bacterial identification was carried out using biochemical characteristic analysis. A total of 12 endophytic bacterial isolates were successfully isolated from leaves, stem and roots of plants. Quantitative test results showed that all isolates can produce IAA. The highest concentration of IAA was produced by B2 (6.51 ppm). Biochemical analysis indicated that the isolates were from the genus Enterobacter, Bacillus, Pseudomonas and Staphylococcus. Endhophytic bacteria that can produce IAA have the potential to be developed as biofertilizers to increase crop productivity.
Aktivitas Inhibitor Lipase Ekstrak Daun Mangga Arum Manis dan Mangga Kweni Secara In Vitro Oktira Roka Aji; Nora Bastiani; Merlia Rahma Tari; Diah Asta Putri
Al-Kauniyah: Jurnal Biologi Vol 17, No 1 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.1.18776

Abstract

 AbstrakObesitas disebabkan adanya kelebihan kalori dalam tubuh. Penurunan obesitas dapat dilakukan dengan cara menghambat penyerapan lemak sebagai sumber utama kelebihan kalori. Salah satu cara yang dapat dilakukan untuk menghambat penyerapan lemak adalah dengan menghambat aktivitas lipase pankreas. Penelitian ini dilakukan untuk menentukan aktivitas inhibitor lipase ekstrak metanol daun mangga arum manis (Mangifera indica L. cv Arum manis) dan kweni (Mangifera odorata Griff.). Tahapan penelitian diawali dengan ekstraksi menggunakan metode maserasi lalu diikuti dengan uji kadar total fenol, kadar flavonoid, dan uji aktivitas inhibitor lipase ekstrak daun mangga arum manis serta kweni. Hasil uji kadar total fenol menunjukkan total fenol pada ekstrak daun mangga arum manis dan kweni berturut-turut yaitu 246,94 mg GAE/g dan 176,11 mg GAE/g. Kadar flavonoid daun mangga arum manis, yaitu 129,95 mg.QE/g, sedangkan daun mangga kweni yaitu 26,50 mg.QE/g. Masing-masing ekstrak diuji kemampuannya dalam menghambat lipase pankreas secara in vitro. Nilai IC50 ekstrak daun mangga arum manis dan kweni, yaitu 61,55 µg/mL dan 79,98 µg/mL. Nilai tersebut lebih tinggi dibandingkan kontrol positif, yaitu orlistat (18,01 µg/mL). Dengan demikian, ekstrak daun mangga arum manis dan kweni dapat menghambat aktivitas lipase pankreas dan berpotensi untuk dikembangkan sebagai antiobesitas.AbstractObesity is caused by excess calories in the body. Reducing obesity can be done by inhibiting the absorption of fat as the main source of excess calories. One way that can be done to inhibit fat absorption is by inhibiting pancreatic lipase activity. This research was conducted to determine the lipase inhibitor activity of methanol extract of arumanis (Mangifera indica L. cv Arumanis) and kweni (Mangifera odorata Griff.) mango leaves. The research stages began with extraction using the maceration method, followed by testing the total phenol content, flavonoid content, and testing the lipase inhibitor activity of arumanis and kweni mango leaf extracts. The results of the total phenol content test showed that the total phenol in Arumanis and Kweni mango leaf extracts was 246.94 mg GAE/g and 176.11 mg GAE/g, respectively. The flavonoid content of Arumanis mango leaves is 129.95 mg.QE/g, while that of Kweni mango leaves is 26.50 mg.QE/g. Each extract was tested for its ability to inhibit pancreatic lipase in vitro. The IC50 values of Arumanis and Kweni mango leaf extracts are 61.55 µg/mL and 79.98 µg/mL. This value is higher than the positive control, namely orlistat (18.01 µg/mL). Thus, Arumanis and Kweni mango leaf extracts can inhibit pancreatic lipase activity and have the potential to be developed as an anti-obesity agent.
Co-Authors Abdul Aziz Adz-Dzikir, Fatihatuljannah Aila Gema Safitri Aisyah, Iis Nisya Nur Al Farisi, Irsyad Andika Bayu Chandra Aninda Kumala Sari Anisa Nurul Huda Ardi, Subhan Zul Arifa Nadya Nur Afifah ARUM PATRIATI Arumbina, Vibula Rosa Astuti, Sutan Nur Chamida Tri Azizah, Mar'atul Azizah, Rara Asti Cahya Ningsih, Ayu Cucu Cahyanti Diah Asta Putri Diah Asta Putri Diah Asta Putri Diah Asta Putri DYSHELLY NURKARTIKA PASCAPURNAMA EDY GIRI-RACHMAN PUTRA Efrida Siti Alifia Ega Asnatasia Maharani ERNAWATI ARIFIN GIRI-RACHMAN Falih, Afifah Nurul FENRYCO PRATAMA Fitria, Karomah Indah Handayani, Erisa Budi Hartanto, Dody Haryadi, Bagus Hasna Chaerani Zakkiyah Hidayah, Hidayah Hudaya, Riris Asyhar IHSANAWATI IHSANAWATI Ihsani, Nisa Ika Maryani Ilma, Nur Malika Imelda Pangaribuan Inggita Utami Irsalinda, Nursyiva Irvanita, Deva Anggun Jannati Aulah Jinan, Rifa Fatma Kamilah, Umu Kurnia, Dhiffa Trisna Laila, Alfiatul Larasati Haliimah Roosyidah Lestari, Iva Dita Lestari, Kinasti Dwi Linda Pertiwi MAELITA RAMDANI MOEIS MAELITA RAMDHANI MOEIS Maliza, Rita Mardiah, Irma Merlia Rahma Tari Muhammad Farid Maksum Nisa Ihsani Nofa Nur Fiani Nora Bastiani Nugraha, Aisyah Aulia Nugraha, Farhan Satya Nur Azizah Nur Azizah Nuraisyah Binte Zainal Nurul Suwartiningsih Pinasti, Vanya Aurel Pratiwi, Ambar Pratiwi, Ambar Puguh Wahyu Prasetyo, Puguh Purwanti Pratiwi Purbosari Puspitarini, Sofia Putri , Diah Asta Putri, Anas Tasya Putri, Aulia Ramanda Putri, Diah Asta Putri, Nida’a Fauziyyah Rinaldi Rizal Putra Riris Asyhar Hudaya Rohmantin, Dita Rossa Puteri Baharie, Sri Safitri, Salsabila Salimah, Azizah Zahratus Santrianda, Aldhiani Setiawan, Haris Silfi Pratiwi Sri Wahyuni Sri Wahyuni Sumbudi, Milliniawati Suwartiningsih, Nurul Suwartiningsih, Nurul Tiwi Sartika Tri Puspita Sari Tufahati, Nadhira Umi Salamah Vianingtyas, Naresh Wari Mega Wachid Eko Purwanto Wicaksono, Adelyanty Pangestuty Wulandari, Endah Retno Yudi Ari Adi, Yudi Ari Yuliani, Ririn Kholifatu Zahra, Safa Auli Zakkiyah, Hasna Chaerani Zul Ardi, Subhan