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All Journal Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Akademika Biologi Microbiology Indonesia Jurnal Biologi Tropis Indonesian Food and Nutrition Progress Jurnal Mikologi Indonesia Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology AL-HAYAT: Journal of Biology and Applied Biology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal Riset Biologi dan Aplikasinya Generics: Journal of Research in Pharmacy Generics : Journal of Research in Pharmacy
MG Isworo Rukmi
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology

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Isolasi dan penapisan bakteri proteolitik endofit tanaman pepaya (Carica papaya L.) Lailatul Mubarokhah; Wijanarka Wijanarka; MG Isworo Rukmi
NICHE Journal of Tropical Biology Vol. 3, No. 2, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.2.99-104

Abstract

Karakterisasi bakteri diazotrof dan pengaruhnya terhadap pertumbuhan tanaman kedelai (Glycine max L. Merrill) Luluk AD Astuti; Dwi Agustiyani Muslichah; Agung Suprihadi; MG Isworo Rukmi; Nani Mulyani; Entis Sutisna
NICHE Journal of Tropical Biology Vol. 4, No. 1, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.1.40-49

Abstract

Characterization of Filoplan and Endophytic Mold Isolates Avicennia marina from Mangrove Area, Semarang Wahyu Aji Mahardhika; Arina Tri Lunggani; Isworo Rukmi; Dicky Setiawan; Ansalakhul Balayatin Ni’mah; Nur Amalia Firdausa; Dina Devina Anggraeni
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2702

Abstract

The presence of fungi in nature is very abundant, but at this time it is still necessary to know and explore the various types that are able to live in various places. Fungi can live in plant tissue, known as endophytic fungi, and can also live on the leaf surface of a plant. Avicennia marina lives on the coast and is used to reduce abrasion, besides being able to live in tidal habitats and high salinity levels. This is supported by the plant's physiology and the help of its microbial symbionts, therefore it is necessary to explore fungi, especially fungi that are able to symbionting with these plants because they are thought to be resistant to high levels of salinity. The purpose of this study was to determine the types of molds that have been isolated and are able to live in the tissue and leaf surface of A. marina. Characterization method was reviewed through macroscopic and microscopic morphology using a microscope and compared with literature from various sources. The characterization results of the 12 isolates obtained several genera, including Trichoderma, Aspergillus, Penicillium, Curvularia, Paecilomyces, Talaromyces, Syncephalastrum, and 1 unidentified isolate. These isolates need to be continued with further research to see their ability to produce metabolites and so on.
Kempong, A Traditional Fermented Food in Karangpucung Kidul village, Linggapura Bumiayu, Central Java: Fermentation Agent and Their Roles Isworo Rukmi; Devia Kusmawati Arfina; Endang Kusdiyantini
Indonesian Food and Nutrition Progress Vol 14, No 1 (2017)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.24257

Abstract

Kempong is a traditional fermented food that is found only in South Karangpucung    Linggapura Bumiayu village, Central Java. It is prepared from palm kernel cake (PKC).  This fermented food is consumed mostly everyday by the people in the village as a side dish or snack. Study on the mold important in the kempong fermentation was done by isolating molds from kempong, PKC, laru and the air of preparation room. Eleven species of molds were successfully isolated from different samples. Among the mold isolates three species came out from kempong product, i.e. R. oryzae, E. chevaliery and A. oryzae. The isolates showed the capability in producing amylase, protease, lipase, and cellulase. Both R. oryzae and A. oryzae indicated as the main fermentation mold in kempong production, because the inoculum laru only contained these two species.
Microbiological Analysis of Liquid Lipstick Products Used by Makeup Artists Chairunnisaa Jabal Rahmah; Sri Pujiyanto; Isworo Rukmi
Al-Hayat: Journal of Biology and Applied Biology Vol 4, No 2 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i2.9326

Abstract

AbstractLipstick is a non-sterile pharmaceutical product that is used for make-up on the lips. Liquid lipstick used by makeup artists may have a high level of microbial contamination because they are applied to different clients in turn. This study aims to determine the microbiological quality of liquid lipstick used by makeup artists based on the result of the Bacterial Total Plate Count (TPC), Yeast and Mold Count (YMC), and the presence of pathogenic microbes. Three samples of liquid lipstick were taken from three different makeup artists and one sample was a new liquid lipstick product. The Bacterial TPC test was carried out on TSA media and the YMC test was carried out on SDA with the spread plate technique. The presence of microbial pathogens P. aeruginosa, S. aureus, and C. albicans were carried out on selective media. The results of bacterial TPC was 9.0 × 103 to 3.0 × 104 CFU/mL, while the YMC was 1.1 × 103 to 3.1 × 103 CFU/mL. The pathogenic microbes detected were S. aureus in 2 samples and C. albicans in 1 sample. The bacterial TPC and YMC values exceed the contamination limit based on BPOM Regulation No. 12/2019.
Berbagai Kapang Aspergillus seksi Nigri dari Buah Kedondong Wahyu Aji Mahardhika; Isworo Rukmi
Jurnal Mikologi Indonesia Vol 6, No 1 (2022): Juni 2022
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v6i1.175

Abstract

Kapang merupakan cendawan berfilamen yang hidup pada berbagai subsrat dan memiliki habitat yang beragam, antara lain hidup pada tanah, air, serealia, endofit dalam tanaman, dan buah. Habitat kapang yang beragam juga memperbanyak diversitas kapang pada berbagai substrat, salah satunya pada buah kedondong. Salah satu kapang yang sering ditemukan adalah Aspergillus. Kapang Aspergillus terdiri dari beberapa grup salah satunya adalah Aspergillus seksi Nigri yang lebih dikenal dengan black mold. Tujuan dari penelitian ini adalah untuk mengidentifikasi kapang Aspergillus seksi Nigri pada buah kedondong yang telah rusak atau busuk. Isolasi kapang tersebut menggunakan metode secara langsung (purposive sampling) dengan mengambil kapang Aspergillus yang diduga berasal dari seksi Nigri, lalu diinokulasikan ke media PDA, MEA, dan CDA. Kapang kemudian diamati morfologinya secara makroskopis dan mikroskopis serta selanjutnya diidentifikasi untuk mengetahui spesiesnya. Pada penelitian ini diperoleh hasil sebanyak sembilan isolat yang berasal dari buah kedondong, diantaranya Aspergillus niger, Aspergillus foetidus, Aspergillus aculeatus, Aspergillus japonicus dan Aspergillus sp. Penelitian selanjutnya isolat tersebut dapat diidentifikasi secara molekuler dan diuji potensinya.
Thermostable alkaline protease activity from Aspergillus flavus DUCC- K225 and its compatibility to local detergents A N S Rini; Isworo Rukmini; S Pujiyanto
Microbiology Indonesia Vol. 15 No. 1 (2021): March 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.09 KB) | DOI: 10.5454/mi.15.1.3

Abstract

Protease is the most important enzyme in many industries, including detergent. Aspergillus flavus DUCC-K225 is a thermotolerant indigenous molds isolated from Madura island which is potential in producing thermostable alkaline protease enzymes. The enzyme produced by submerged culture on modified Czapeks Dox liquid medium containing glucose as carbon source and 1% of casein. The aims of this study were to determine the activity and stability of thermostable alkaline protease produced by A. flavus DUCC-K225 at various temperatures, also the compatibility to 5 local detergents. Research were done using CRD, with temperature variation for protease activity as treatment. The results showed that the optimum activity of thermostable alkaline proteases was 214.503 U/mL, with retained activities up to 78% in 60 minutes at 55°C. The alkaline thermostable protease compatible with 5 local detergents tested, with the retained activity varied 55.8%-95.22% at 29oC and 62.36%-97.7% at 55oC. The compatibility to detergent confirmed by blood stained cleansed. The higher the protease activity, the higher the blood stain solubility. Key words: Alkaline protease, thermostable, A. flavus DUCC-K225, compatibility, detergent
Biodiversity of Costus speciosus Phylloplane Fungi in Baturaden Botanical Gardens and Antagonist Testing against Fusarium oxysporum Aulia, Muhammad Hilman; Rukmi, Isworo; Suprihadi, Agung; Mahardhika, Wahyu Aji
Jurnal Riset Biologi dan Aplikasinya Vol. 6 No. 1 (2024)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v6n1.p41-50

Abstract

Indonesia has an abundant biodiversity of natural resources, one of which is phylloplane fungi. This abundant phylloplane mold needs further investigation to utilize its potential as an antagonistic agent. This study aims to prove the existence and to determine the types of phylloplane fungi species found on the leaf surface of pacing tawar (sweet ginger or crepe-ginger) plants, to analyze and determine the level of diversity and dominance of phylloplane, and to determine the types of phylloplane fungi species that have the antagonistic ability of the phytopathogenic mold Fusarium oxysporum. This research method includes isolating crepe-ginger phylloplane fungi using a contact plate, isolates purification, macromorphological and micromorphological identification, diversity and dominance index calculation, and antagonist test using a dual culture method. This study used a descriptive analysis research design. The isolation results obtained 57 species of crepe-ginger plants leaf phylloplane molds consisting of eight genera (Alternaria, Aspergillus, Cladosporium, Curvularia, Fusarium, Mucor, Penicillium, and Trichoderma). The results of the diversity index calculation with a value of 2.02 showed that the diversity of phylloplane fungi was moderate. The result of the dominance index calculation, with a value of 0.1521, shows no tendency for phylloplane fungi species to dominate. The results of the antagonist test showed that 15 isolates could antagonize the F. oxysporum with an inhibitory proportion of up to 82% obtained from T. viride isolates with code FD5B3.2
Formulasi Sediaan Spray Gel SMEDDS Ekstrak Etanol 96% Daun Jambu Biji (Psidium guajava L.) sebagai Antibakteri Terhadap Staphylococcus aureus Ekawati, Nuraini; Nuraeni, Pratika; Rukmi, Isworo
Generics: Journal of Research in Pharmacy Vol 4, No 2 (2024): Generics : Journal of Research in Pharmacy, Volume 4, Edisi 2, 2024
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/genres.v4i2.20392

Abstract

Staphylococcus aureus merupakan bakteri penyebab infeksi kulit dan pada ulkus diabetikum, sehingga dibutuhkan antibakteri untuk mencegahnya. Daun jambu biji (Psidium guajava L.)  mengandung antimikroba. Spray gel mencegah infeksi silang, praktis, dan meningkatkan penetrasi obat. Kombinasi Na CMC dan kopovidon menghasilkan spray gel yang stabil dan membentuk lapisan film elastis. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi konsentrasi Na CMC dan kopovidon sebagai gelling agent terhadap karakteristik fisik spray gel SMEDDS ekstrak etanol 96% daun jambu biji dan aktivitas antibakterinya. Simplisia daun jambu biji dimaserasi dengan etanol 96%, kemudian dibuat SMEDDS, dan diformulasikan dalam spray gel dengan memvariasikan Na CMC dan kopovidon 1:1 (F1=0,1%, F2=0,2%, F3=0,3%). Uji KHM terhadap Staphylococcus aureus dilakukan pada ekstrak. Karakterisasi fisik dilakukan terhadap spray gel. Aktivitas antibakteri spray gel dilakukan dengan metode Kirby Bauer. Hasil yang didapat, yaitu KHM ekstrak sebesar 0,5%. Konsentrasi Na CMC dan kopovidon tidak berpengaruh terhadap karakteristik fisik spray gel. F2 dan F3 memenuhi semua peryaratan karakteristik fisik, sedangkan F1 tidak memenuhi persyaratan viskositas. Spray gel F1, F2, dan F3 memiliki aktivitas antibakteri terhadap Staphylococcus aureus berturut turut sebesar 28,10; 22,35; dan 22,09 mm, tetapi berbeda tidak bermakna dengan kontrol negatif.
Optimasi Cmc Na dan Gliserin pada Formula Gel Ekstrak Etanol Daun Kersen (Muntingia calabura L.) dan Uji Aktivitas Antibakteri Terhadap Staphylococcus aureus Handayani, Salsabila Putri; Ekawati, Nuraini; Rukmi, MG Isworo
Generics: Journal of Research in Pharmacy Vol 4, No 1 (2024): Generics : Journal of Research in Pharmacy, Volume 4, Edisi 1, 2024
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/genres.v4i1.20393

Abstract

 Ulkus diabetik dari diabetes mellitus berupa luka terbuka pada permukaan kulit dan adanya kematian jaringan tubuh. Bakteri patogen paling utama ditemukan pada ulkus diabetik, yaitu Staphylococcus aureus. Daun kersen (M. calabura L.) dilaporkan mengandung senyawa metabolit sekunder yang berpotensi sebagai agen antibakteri dan diketahui ekstrak etanol 70% daun kersen (M. calabura L.) memiliki aktivitas antibakteri terhadap S. aureus. Gel mengandung komponen air yang lebih tinggi dibandingkan sediaan topikal lainnya. CMC Na dan gliserin digunakan sebagai gelling agent dan humektan. Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi CMC Na dan gliserin terhadap karakterisitik gel dan aktivitas antibakteri terhadap S. aureus, serta mengetahui formula optimum sediaan gel ekstrak etanol 70% daun kersen (M. calabura L.) dengan variasi konsentrasi CMC Na dan gliserin yang memiliki karakteristik fisik gel dan aktivitas antibakteri yang baik. Simplisia daun kersen dimaserasi dengan etanol 70%, kemudian maserat dikentalkan dengan rotary evaporator. Gel diformulasikan dengan memvariasikan konsentrasi CMC Na dan gliserin, lalu dikarakterisasi fisik dan uji aktivitas antibakteri. Penentuan formula optimum dilakukan menggunakan design expert. Hasil yang didapatkan yaitu variasi konsentrasi CMC Na dan gliserin berpengaruh signifikan pada pH, daya lekat, dan daya sebar, tetapi tidak berpengaruh signifikan pada aktivitas antibakteri terhadap S. aureus. Formula optimum gel ekstrak etanol daun kersen adalah kombinasi konsentrasi CMC Na 2,234% dan gliserin 11,766%. 
Co-Authors A N S Rini Agung Suprihadi Agung Suprihadi Ahmad Thontowi Anam, M. Khairul Ansalakhul Balayatin Ni’mah Anto Budiharjo Arina Tri Lunggani Arina Tri Lunggani Arum Krisna Miranti, Arum Krisna Astuti, Luluk Aviyani Dwi Aulia, Muhammad Hilman Budi Raharjo Budi Raharjo Budihardjo, Kadarwati Chairunnisaa Jabal Rahmah Chatarina Umbul Wahyuni Dahniar Saraswati Dani Sukma Saefunida Devia Kusmawati Arfina Devia Kusmawati Arfina Diani Ajeng Prahesti Diani Kurniawati Dicky Setiawan Dina Devina Anggraeni Dwi Agustiyani Muslichah Ekawati, Nuraini Elvi Yetti Endang Kusdiyantini Endang Kusdiyantini Enny Fachriyah Entis Sutisna Fathika Fitrania Fathika Fitrania Febrianty, Debby Ananda Fitri Wulandari Ginting, R Cinta Badia Griffin Natassya Handayani, Salsabila Putri Hapzi Ali Helga Lusiana, Helga Hermin Pancasakti Kusumaningrum Indra Prawira Jepri Agung Priyanto, Jepri Agung Karima, Radhita Khodijah Baharun Khoirin Nisa Khoirin Nisa Kristina Kristina Laelatul Baniyah Lailatul Mubarokhah Larasati, Ella Dewani Luluk AD Astuti Luthfian Nur Afifi Lynda - Sutrisna Mahardhika, Wahyu Aji Mangasa Tua Pandapotan Siregar Mulyani, Nies S Muslichah, Dwi Agustiyani Nani Mulyani Natasia, Novera Nopvita Windi Astuti Norma Sainstika Pangestu Novik Nur Hidayat Nur Amalia Firdausa Nuraeni, Pratika Nurcahyo, Rizky Pitaloka, Gendis Angger Prabhata, Wimzy Rizqy Putra, Mohammad Affan Dwica Putri Ramadhani Rahmah, Chairunnisaa Jabal Ratna Cempaka Lingga Rejeki Siti Ferniah Ridho Mathori Ikhwan Ristia Rachmatunnisa Rohana, Evieta S Pujiyanto Sabrini, Zalia Sari, Anisa Rachma Sasikirana, Widyandani Sekar Ayu Yogyaswari Septiani, Arom Sesaria Esa Sekar Ardini Siregar, Mangasa Tua Pandapotan Sitepu, Harum Siti Nur Jannah Siti Nur Jannah Siti Nur Jannah Soowanayan, Chumporn Sri Pujiyanto Sri Pujiyanto Suetrong, Satinee Susiana Purwantisari Utami, Linda Ayu w wijanarka Wahyu Aji Mahardhika Wahyu Aji Mahardhika Whinarsih, Whinarsih Widayu Mutiya Ramadhani Wijanarka Wijanarka Wijanarka, W Yopi Yopi