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All Journal Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Akademika Biologi Microbiology Indonesia Jurnal Biologi Tropis Indonesian Food and Nutrition Progress Jurnal Mikologi Indonesia Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology AL-HAYAT: Journal of Biology and Applied Biology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal Riset Biologi dan Aplikasinya Generics: Journal of Research in Pharmacy Generics : Journal of Research in Pharmacy
MG Isworo Rukmi
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology

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AKTIVITAS ANTAGONISTIK KAPANG ENDOFIT DUWET (Syzygium cumini (L.) Skeels) TERHADAP Alternaria porri PENYEBAB BERCAK UNGU PADA BAWANG MERAH (Allium ascalonicum L.) SECARA IN-VITRO Ristia Rachmatunnisa; MG. Isworo Rukmi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Endophytic fungi has been capable in producing secondary metabolites similar to those produced by its host. Secondary metabolites in some parts of duwet tree showed an antifungal activity. The aims of this study were to determine the ability of duwet endophytic fungi in inhibiting A. porri fungus, a pathogenic agent for  purple blotch disease on onion. The experiment conducted using CRD with eight endophytic fungi isolates as treatment with three replications. Antagonistic activity observed using dual culture method. The endophytic fungal isolates were conventionally identified to genus level. The results showed that endophytic fungi were identified as five isolates of Penicillium and one isolate of Aspergillus, Fusarium and mycelia sterilia. The antagonistic acitivity of duwet endophytic fungi against A. porri were varied between 18.1% - 47.3%. The highest antagonistic activity showed by Fusarium JD1 (47,3%). Keywords: Alternaria porri, purple blotch, onion disease, antagonistic fungi, endophytic fungi.
Produksi Enzim Protease Aspergillus Flavus Pam-25 Dengan Variasi Ph Dan Waktu Inkubasi Indra Prawira; MG. Isworo Rukmi; w wijanarka
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Enzyme is a biokatalisator which can increase the speed of the reaction without join react. Protease enzyme is one of the enzymes that have a high economic value because its application is extensive in the field of industry. Protease is an enzyme that catalyzes the proteolytic peptide bonds in proteins termination. This research aims to determine the effect of pH and incubation time on the production of protease enzyme from A. flavus PaM-25.. This research aims to know the influence of the variation of the pH of the incubation time and against the production of the enzyme protease of A. flavus PaM-25. The research  was conducted at the laboratories of Microbiology Department of Biology, Faculty of Science and Mathematics, University of Diponegoro. Variables observed were protease activity, protein content and specific activity. Research using randomized complete block design (RAK) with two factors. The first factor is variation of pH, P1 (pH 7), P2 (pH 8), and P3 (pH 9), while the second factor is the incubation time T5 (5th day), T6 (6th day) and T7 (7th day) with repeated 3 times. Research data analyzed by Analysis of variance (ANOVA) and continued by Least Significant Difference test and Duncan Significant Difference Test at test level 5%. The results showed that the Aspergillus flavus PaM-25 has the capability of producing alkaline protease enzymes with pH range 7.0-9.0. The highest activity of proteases of   A. flavus PaM-25 retrieved on 7th day incubation time of treatment with protease activity value 1.94 U/mL, while pH treatment has no effect. The highest levels of protein found in the treatment of pH 7 and 5th days of incubation time of 1.05 mg/mL. The value of the highest purity of enzymes found in the combination of treatment pH 9 and 7th day incubation time  of 12.91 U/mg protein
ISOLASI BAKTERIOFAG Escherichia coli DARI SISTEM DISTRIBUSI AIR MINUM ISI ULANG SEBAGAI ANTIBIOFILM Dani Sukma Saefunida; W Wijanarka; MG Isworo Rukmi; Novik Nur Hidayat
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

E. coli termasuk golongan bakteri koliform sebagai indikator kualitas air yang dapat membentuk biofilm pada sistem distribusi air minum isi ulang. Biofilm tersebut dapat menyebabkan kontaminasi dan penyebaran penyakit. Bakteriofag yang memiliki kemampuan dalam melisiskan inang dapat dijadikan solusi permasalahan tersebut. Tujuan penelitian adalah untuk mengisolasi bakteriofag E. coli dari sistem distribusi air minum isi ulang dan menguji aktivitas antibiofilm. Isolasi bakteriofag dilakukan dengan metode plaque assay, sedangkan uji aktivitas antibiofilm menggunakan metode microtiter plate assay. Sampel yang digunakan adalah biofilm dari pipa sumber air, tangki penyimpanan depot air minum dan produk air minum isi ulang. Hasil penelitian menunjukkan bakteriofag E. coli dapat diperoleh dari masing-masing sampel dan memiliki aktivitas antibiofilm. Kata Kunci : Antibiofilm, Bakteriofag, E. coli.
ANALISIS MIKOFLORA DALAM MAKANAN FERMENTASI TRADISIONAL KEMPONG DI DESA KARANGPUCUNG KIDUL, LINGGAPURA BUMIAYU JAWA TENGAH Devia Kusmawati Arfina; Endang Kusdiyantini; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 2 No. 1 Januari 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Kempong is a traditional fermented foods which is traditionally made of palm kernel cake substrate from the South Karangpucung Linggapura Bumiayu village, Central Java. This study is aimed to identify a mold which has a role in the fermentation process and testing in an activity of Kempong’s enzyme from mycoflora obtained. Proximate analysis of the samples of mold and palm kernel cake are conducted to determine the nutrient content of the substrate and fermentation products. The Results isolation, from the environment, substrate, laru and product, show 14 isolates of  molds, there are R.oryzae, A. niger Van Tieghem, A. carbonarius, Geotrichum candidum, A. ochraseus, Rhizomucor sp, A. chevalieri, A. tamarii, A. oryzae, A. flavus, A. nidulans, A. fumigatus, A. glaucus, and A. parasiticus. R. oryzae is a mold found in every material examined. It indicates that the fermentation is done mainly soothers by    R. oryzae. Proximate analysis of the kempong, shows a levels of carbohydrate 16.67%, 74.03% water, 0.75% ash, 2.80% fat and 5.77% protein. Nutrients content except water are lower than the substrate palm kernel cake. Decreasing of protein, fat, and carbohydrate fermentation are caused by R. oryzae. Keywords: kempong, mycoflora, enzyme activity, proximate analysis.
UJI ANTAGONIS KAPANG ENDOFIT DUWET (Syzigium cumini (L.) Skeels) TEHADAP KAPANG Fusarium oxysporum PENYEBAB PENYAKIT MOLER PADA BAWANG MERAH (Allium ascalonicum L.) SECARA IN-VITRO Luthfian Nur Afifi; MG. Isworo Rukmi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Endophytic fungi inhabit plant hosts for all or part of their life cycle in plant tissues without doing harm to its host. Utilization of endophytic fungi as controlling plant pathogens has been widely studied in in-vivo and in-vitro. The aims of this study was to determined the antagonistic activities of 8 endophytic fungal isolates from some parts of duwet (Syzigium cumini (L.) Skeels.) tree against F. oxysporum, a pathogenic fungi causing bulb rot disease on red onion. This study was conducted using Completely Randomized Design (CRD), with 8 endophytic fungal isolates as treatment, done in triplicates. The antagonistic activity were examined using dual culture method, by determined the percentage of inhibition. The mycelial plugs (4 mm diameter) of endophytic fungi and pathogenic fungi F. oxysporum were placed in same dish 3 cm each other. The endophytic fungal isolates were conventionally identified to genus level. The identification results showed that endophytic fungi came from 1 isolates of Aspergillus, 5 isolates of Penicillium, 1 isolate of Fusarium, and 1 isolate of Mycelia sterilia. All endophytic fungal isolates showed capability on inhabiting the growth of F. oxysporum with the percentage of inhibition varied between 1.1 to 36.0%. Fusarium JD1 isolates showed the highest antagonist activity against F. oxysporum (36.0%). Keywords : Endophytic fungi, duwet, Fusarium oxysporum, growth inhibition.
PENGARUH CaCl2.2H2O DAN WAKTU INKUBASI TERHADAP PRODUKSI INULINASE OLEH Pichia manshurica DUCC Y-015 DALAM SUBSTRAT TEPUNG UMBI DAHLIA Dahniar Saraswati; W Wijanarka; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Fructose production from inulin by inulinase only need one reaction enzimatic step and produce 95% fructose. Inulin obtained from dahlia tuber and inulinase produced by Pichia manshurica DUCC Y-015. Inulinase production (E.C. 3.2.1.7.) can be influenced by metal salt suplementation, such as CaCl2.2H2O. The purpose of this research were to known the influence of CaCl2.2H2O and incubation time to inulinase production by P. manshurica DUCC Y-015 on Dahlia Tuber Substrate. The design that use in this research were Randomized Factorial Block Design ( RAFBD ). Factor I (CO, C­1, C2, C3) as the concentration of CaCl2.2H2O (0 mM, 0.25 mM, 0.50 mM, 1.00 mM) and Factor II ( T12, T18, dan T24 ) as incubation time ( 6, 12, 18 hour), the repetition were 3 times. The result analyze by ANOVA (Analysis of Variance) and continued by LSD test. The result of this research indicate that CaCl2.2H2O and incubation time were not significantly influence to inulinase production. The highest inulinase production by Pichia manshurica DUCC Y-015 indicate by C2T12 treatment which use 0.50 mM CaCl2.2H2O and 12 hour incubation time, the enzyme activity is 0.60 IU/mLKey Words : CaCl2.2H2O, Dahlia tuber,  Incubation time, Inulin, Inulinase, Pichia manshurica DUCC Y-015.
Identifikasi Isolat Aspergillus sp. KRM 43 dari Madura dan Produksi Enzim Protease dengan Variasi pH dan Waktu Inkubasi Nopvita Windi Astuti; MG. Isworo Rukmi; w wijanarka
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Proteases is an enzyme that has a high economic value, because they widely used for application in the field of industry.Protease can be generated from microorganisms, one of protease producer is derived from the genus Aspergillus. Aspergillus sp. KRM 43 that has isolated from alkaline soil can be predicted capable to produce alkaline protease. The purpose of this study was  to know the optimum of pH and incubation time for enzyme production from the mould. This research was conducted using a  factorial Randomized Complete Block Design with two factor i.e. a pH of 7, 8, 9 and incubation time of 5, 6, 7 days. Data collected were analyzed using Anova (α 0,05). The mould isolate were identified by observing its macroscopic and microscopic morphology. The result showed that Aspergillus sp. KRM 43 was identified as Aspergillus parasiticus. In this study showed pH treatment hadn't been  able to affect the production of protease. The highest proteases alkaline production of A. parasiticus KRM 43, found at 7 days incubation with proteases activity 2.24 U/mL and the specific activity 7.23 U/mg.Key words: Proteases, Aspergillus parasiticus, pH, incubation time
EKPLORASI BAKTERI SELULOLITIK DARI CAIRAN RUMEN SAPI PERANAKAN FRIES HOLLAND (PFH) DAN LIMOUSINE PERANAKAN ONGOLE (LIMPO) Sekar Ayu Yogyaswari; MG. Isworo Rukmi; Budi Raharjo
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cellulose is the largest component of plant materials which is the most abundant organic compound in nature. The degradation of cellulose carried out by cellulase enzymes which is consisting of three components, i.e. , endoglucanase, exoglucanase, and β-glucosidase with glucose as the end product. Cellulase is very useful in industry and agriculture, such as paper, beer and brewing industries, for improving the quality of forages, organic material decomposer, and play an important role in the bioconversion of cellulose into various chemical commodities. The aims of this study was to get cellulolytic bacteria from cow’s rumen fluid which has high cellulolytic activity. Isolation of cellulolytic bacteria from cow’s rumen fluid were done by direct plating on CMC media. The cellulolytic activity values of the bacterial isolates were examined by determining cellulolytic index. The determination of cellulase activity were carried out by DNS method while the total protein contents by Lowry method. Identification of the bacteria isolates were done by observation of colony morphology, microscopic observation, and biochemical tests. Six potential cellulolytic bacterias were obtained in this study and all isolates identified as Bacillus. The highest cellulase specific activity shown by Fh-9 and Lo-8 isolates in 8 hours incubation.Keywords: cellulolytic bacteria, cellulose, cellulase.
KEANEKARAGAMAN DAN AKTIVITAS ENZIMATIS KAPANG RIZOSFER KACANG MEONGAN (Aeschynomene americana L.) DI DESA SUKOLILO BARAT, KECAMATAN LABANG, KABUPATEN BANGKALAN, MADURA Griffin Natassya; Agung Suprihadi; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Molds are widely distributed in nature, they even present in extreme environment, such as hot and dry soil. Molds which can grow in extreme environment has been adapted to xeric environment by producing enzymes  with  special  characteristics.  The  aim  of  this  study  to  determine  the  diversity  of  molds  from A.americana L. rhizosphere at West Sukolilo Village, Labang district, Bangkalan Regency, Madura and also to examined their cellulolytic and proteolytic activities. Isolation of molds were done by using spread and dilution methods. Molds identification were done by macroscopic and microscopic examined. The molds diversity was calculated using Shannon-Wiener index. Semiquantitative enzyme examination were done using Carboxymethil Cellulose (CMC) agar for cellulolytic ang liquid gelatin 12% for proteolytic. Results of this study showed that mold diversity isolated from   A. americana L. rhizosphere was moderate (1,8-2,7) with total of 43 species, comes from 7 genus i.e. Aspergillus, Penicillium, Fusarium, Aureobasidium, Byssochlamys, Paecilomyces, and Trichoderma.  The  highest  index  of  cellulolytic  produced  by  Aspergillus  sydowii  (2,35),  while  highest proteolytic activity produced by Aspergillus flavus (86%). Keywords : molds, diversity index,xeric, cellulolytic, proteolytic
KERAGAMAN BAKTERI ASAM LAKTAT SECARA MOLEKULER PADA ILEUM DAN SEKUM AYAM BROILER YANG DIBERI PAKAN PREBIOTIK BEKATUL DAN BEKATUL HASIL FERMENTASI Laelatul Baniyah; Siti Nur Jannah; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lactic Acid Bacteria (LAB) is a digestive tract microflora that play a positive role in poultry health. The number and diversity of LAB in the digestive tract affected by several factors, among them was the kind of feed. The purpose of this research was  to know the molecular diversity of Lactic Acid Bacteria (LAB) in broiler’s ileum and cecum  after  feeding with  prebiotic bran and Rhizopus oryzae fermented  bran which was added to commercial feed. The molecular analysis was done using T-RFLP method , Hae III and Msp I were used as restriction enzymes.  The number of phylotype, relative abundance, Shannon diversity index (H '), evenness (E), and Dominance (D)  were examined . The results indicated that the addition of bran prebiotics on commercial feed showed a higher  diversity of lactic acid bacteria on broiler’s ileum and cecum, compared  with the addition of Rhizopus oryzae fermented  bran. The dominant BAL types are Lactobacillus spp, L. delbrueckii subs. Bulgaricus, L. intermedius, L. amilovorus, uncultured bacteria 87 bp, 280 bp, 331 bp and unidentified bacteria 74 bp, 82 bp, 131 bp. Keywords: Diversity, Lactic Acid Bacteria, Prebiotics, Bran, T-RFLP
Co-Authors A N S Rini Agung Suprihadi Agung Suprihadi Ahmad Thontowi Ansalakhul Balayatin Ni’mah Anto Budiharjo Arina Tri Lunggani Arina Tri Lunggani Arum Krisna Miranti, Arum Krisna Astuti, Luluk Aviyani Dwi Aulia, Muhammad Hilman Budi Raharjo Budi Raharjo Budihardjo, Kadarwati Chairunnisaa Jabal Rahmah Chatarina Umbul Wahyuni Dahniar Saraswati Dani Sukma Saefunida Devia Kusmawati Arfina Devia Kusmawati Arfina Diani Ajeng Prahesti Diani Kurniawati Dicky Setiawan Dina Devina Anggraeni Dwi Agustiyani Muslichah Ekawati, Nuraini Elvi Yetti Endang Kusdiyantini Endang Kusdiyantini Enny Fachriyah Entis Sutisna Fathika Fitrania Fathika Fitrania Febrianty, Debby Ananda Fitri Wulandari Ginting, R Cinta Badia Griffin Natassya Handayani, Salsabila Putri Hapzi Ali Helga Lusiana, Helga Hermin Pancasakti Kusumaningrum Indra Prawira Jepri Agung Priyanto, Jepri Agung Karima, Radhita Khodijah Baharun Khoirin Nisa Khoirin Nisa Kristina Kristina Laelatul Baniyah Lailatul Mubarokhah Larasati, Ella Dewani Luluk AD Astuti Luthfian Nur Afifi Lynda - Sutrisna Mahardhika, Wahyu Aji Mangasa Tua Pandapotan Siregar Mulyani, Nies S Muslichah, Dwi Agustiyani Nani Mulyani Natasia, Novera Nopvita Windi Astuti Norma Sainstika Pangestu Novik Nur Hidayat Nur Amalia Firdausa Nuraeni, Pratika Nurcahyo, Rizky Pitaloka, Gendis Angger Prabhata, Wimzy Rizqy Putra, Mohammad Affan Dwica Putri Ramadhani Rahmah, Chairunnisaa Jabal Ratna Cempaka Lingga Rejeki Siti Ferniah Ridho Mathori Ikhwan Ristia Rachmatunnisa Rohana, Evieta S Pujiyanto Sabrini, Zalia Sari, Anisa Rachma Sasikirana, Widyandani Sekar Ayu Yogyaswari Septiani, Arom Sesaria Esa Sekar Ardini Siregar, Mangasa Tua Pandapotan Sitepu, Harum Siti Nur Jannah Siti Nur Jannah Siti Nur Jannah Soowanayan, Chumporn Sri Pujiyanto Sri Pujiyanto Suetrong, Satinee Susiana Purwantisari Utami, Linda Ayu w wijanarka Wahyu Aji Mahardhika Wahyu Aji Mahardhika Whinarsih, Whinarsih Widayu Mutiya Ramadhani Wijanarka Wijanarka Wijanarka, W Yopi Yopi