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Studi Perbandingan Histopatologi Udang Vaname yang Terinfeksi Vibrio parahaemolyticus dari Tiga Tambak Berbeda Khusnah, Asmaul; Satyantini, Woro Hastuti; Amin, Muhammad
Grouper Vol. 14 No. 2 (2023): Grouper: Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v14i2.212

Abstract

Udang vaname merupakan salah satu jenis udang yang memiliki nilai ekonomis tinggi. Hal ini dikarenakan banyaknya permintaan konsumen terhadap udang vaname. Namun budidaya udang vaname tidak mudah, komoditas tersebut mudah terserang penyakit apabila padat tebar terlalu tinggi dan kualitas air yang buruk. Kualitas air yang buruk dapat mengakibatkan imunitas udang vaname menurun dan mengakibatkan mudah terserang penyakit salah satunya Acute Hepatopancreatic Necrosis Disease (AHPND). Penyakit tersebut dilaporkan salah satunya diakibatkan oleh adanya barkteri Vibrio parahaemolyticus yang menginfeksi udang. Tujuan penelitian ini adalah untuk mengetahui kerusakan jaringan udang vaname yang terinfeksi bakteri Vibrio parahaemolyticus di tiga tambak yang berbeda. Metode yang digunakan pada penelitian ini adalah purposive sampling dengan metode observasi. Teknik pengumpulan data dilakukan dengan cara observasi lapang, yakni mengumpulkan data-data primer dan sekunder di lapangan. Data diambil dari tiga lokasi, yaitu Tuban, Sidoarjo dan Pasuruan. Hasil histopatologi dilakukan skoring kerusakan kemudian dianalisis dengan IBM SPSS menggunakan Uji Kruskal Wallis dan dilanjutkan Uji Z. Berdasarkan hasil penelitian menunjukkan bahwa kerusakan jaringan pada insang dan hepatopankreas akibat infeksi dari bakteri V.parahaemolyticus memiliki kerusakan yang cukup parah. Kerusakan yang terjadi pada insang mulai dari fusi lamella dan edema. Sedangkan kerusakan yang terjadi pada hepatopankreas adalah peluruhan sel dan infiltrasi. Maka dapat disimpulkan bahwa ketiga tempat yang terinfeksi V.parahaemolyticus terdapat perbedaan tingkat kerusakan jaringan hepatopankreas dan insang pada udang vaname yang terjadi pada hepatopankreas dan insang banyak hingga sangat banyak. Kerusakan terbanyak terjadi pada sampel udang yang berasal dari Kabupaten Sidoarjo
Potential of Antioxidant Activity Caulerpa racemosa Extract Using DES Solvent and Different Sonication Times as An Antibacterial Against Pathogenic Bacteria Satyantini, Woro Hastuti; Mukti, Akhmad Taufiq; Bakhri, Saiful
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.53303

Abstract

Caulerpa racemosa, a type of green algae, has been studied  for its potential health benefits, including antioxidant and an- tibacterial properties. The extraction of bioactive compounds  from marine algae using green solvents like Deep Eutectic Sol- vents (DES) has gained interest due to their efficiency and en- vironmental friendliness. This research aimed to determine  the potential antioxidant activity of C. racemosa extract using DES solvents and different sonication times as an antibacterial  against pathogenic bacteria. The research employed a Facto- rial Completely Randomized Design (CRD) with two variables:  sonication time (10, 20, and 30 minutes) and different DES solvents (ethanol and ethylene glycol (1:20) with 90% aquades, and methanol and glycerol (1:10) with 90% aquades. The highest antioxidant activity was found in DES  Methanol-Glycerol (36.39 mg TE/g dw), followed by DES Eth- anol-Ethylene glycol (28.30 mg TE/g dw), both significantly  different (p<0.05). The C. racemosa extract inhibited the growth of V. parahaemolyticus with an inhibition zone of 8.08 mm at 50 ppm and 13.13 mm at 1,000 ppm. For V. harveyi, inhibition started at 6.37 mm at 50 ppm, reaching 11.70 mm  at 1,000 ppm. DES Methanol-Glycerol with a 10-minute soni- cation provided the highest antioxidant activity and effectively  inhibited V. parahaemolyticus and V. harveyi. Extraction of C. racemosa with DES-MG solvent and varying sonication times influences its antioxidant activity and antibacterial properties.
Optimizing Tilapia (Oreochromis niloticus) Growth through a Comparative Evaluation of EM4 and Homemade Probiotics in Pellets Muhammad Izzudin Syaifullah; Satyantini, Woro Hastuti
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.54414

Abstract

One alternative approach to facilitate the utilization of feed by tilapia is the addition of probiotic in feed. In this study, we compare the effects of commercial probiotic EM4 and homemade probiotics on tilapia growth. This research was conducted in PT. Sahaja Berkah Abadi at wonosalam, jawatimur, indonesia. The study was designed as a completely randomized design with 3 treatments and 4 replications, encompassing control group (C), commercial probiotics (G1), and homemade probiotic (G2). The commercial probiotics (G1) contained Rhodopseudomonas sp., Lactobacillus sp., Streptomyces sp., and Actinomycetes sp. while the homemade probiotic (G2) contained starter bacteria Lactobacillus casei and herbal plant such as ginger, curcuma, and tumeric. Parameters observed during the study included weight gain (WG), specific growth rate (SGR), and survival rate (SR). The results revealed that both G1 and G2 performed better than the control group. The weight gain for the control, G1, and G2 groups were 2.38±0.03 g, 3.09± 0.01 g, and 3.13 ± 0.03 g, respectively. Similarly, the specific growth rates for the control, G1, and G2 groups were 4.25±0.03%, 5.52± 0.02%, and 5.59 ± 0.03%, respectively, while the survival rate remained at 100% across all groups. The water quality in all treatments during maintenance met the standard requirements for tilapia, with temperatures ranging from 25.5 to 26.7 °C and pH levels between 6.7 and 6.9. These findings suggest that the probiotics incorporated in G2 present a cost-effective alternative to those in G1, offering a promising option for tilapia cultivation.
Nutrient Concentration, Water Brightness, Chlorophyll-a, and Phytoplankton Abundance as Indicators for Determining the Trophic Status of Lake Toba, North Sumatera - Indonesia Saragih, Friska Setiawani; Djamil, Hasim; Juanda, Juanda; Mubarak, Ahmad Shofy; Hasan, Veryl; Sihombing, Agusmanto; Satyantini, Woro Hastuti
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.57981

Abstract

The purpose of this study was to investigate the water quality and phytoplankton abundance in Lake Toba to determine its trophic status. This research was conducted from January to March 2024 at four stations in Lake Toba. Water samples were taken using a UWITEC water sampler and a plankton net and brought to the laboratory for analysis. The study measured physical and chemical water quality and phytoplankton abundance. The results showed a brightness ranging between 5.8 and 6.3 meters, chlorophyll-a content <10 µg/L, total nitrogen <750 µg/L, and phosphorus ranging from 20 µg/L to 30 µg/L. Based on these parameters, the nutrient quality of Lake Toba was classified as mesotrophic. Phytoplankton abundance ranged from 22,514 to 33,475 individuals/L, with 33 genera identified across nine classes: Bacillariophyceae, Chlorophyceae, Cyanophyceae, Coscinodiscophyceae, Dinophyceae, Euglenida, Fragilariophyceae, Mediophyceae, and Zygnemophyceae. The diversity index (H') was 2.631 - 2.723, the uniformity index (E) was 0.828 - 0.872, and the percentage of blue-green algae was 14.75 - 20.85. Zygnemophyceae was the most dominant phytoplankton class, with Staurastrum sp. being the most prevalent species. In conclusion, Lake Toba's waters are categorized as mesotrophic based on the chemical parameters and as eutrophic based on phytoplankton abundance, indicating potential nutrient enrichment issues that warrant further investigation.
PENDAMPINGAN MANAJEMEN KUALITAS AIR DAN APLIKASI PROBIOTIK PADA BUDIDAYA IKAN LELE PONDOK DARUL MUKMIN, SUKOREJO, PASURUAN JAWA TIMUR Dewi, Nina Nurmalia; Satyantini, Woro Hastuti; Isroni, Wahyu; Mahasri, Gunanti; Manan, Abdul; Cahyoko, Yudi; Wisudyawati, Dita
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i3.2955

Abstract

Pasuruan Regency is one of the regencies that has potential in the fisheries sector, especially freshwater fisheries cultivation. The potential area for cultivation activities is located in Sukorejo District with the cultivated commodity being catfish. One of the farmer groups in Sukorejo District is the Pondok Darul Mukmin Sangkuriang Catfish farmer. However, this catfish cultivation activity has not been running optimally, because the cultivation system used is still traditional. This community service activity aimed to provide assistance related to water quality management and probiotic application in catfish cultivation. This activity was carried out at Pondok Darul Mukmin from July to September 2024. The method of implementing the activity was in the form of training, assistance, and demonstrations of water quality management, probiotic application in feed, and probiotic culture for catfish cultivation. The results of this community service activity showed that Pondok Darul Mukmin fish farmer  were very enthusiastic in participating in the training activities. In addition, the community can also carry out catfish cultivation by monitoring water quality and applying probiotics to feed. Cultivation activities resulted in increased catfish growth every week.
PENGARUH WAKTU PANEN DAN NUTRISI MEDIA TERHADAP BIOPIGMEN Spirulina platensis setyaningsih, Iriani -; Tarman, Kustiariyah -; satyantini, Woro hastuti; barus, dita aguistina
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 16 No. 3 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.596 KB) | DOI: 10.17844/jphpi.v16i3.8054

Abstract

Spirulina platensis merupakan alga hijau biru yang mengandung biopigmen fi kosianin dan antioksidanyang bermanfaat bagi kesehatan. Produksi biopigmen dipengaruhi oleh nutrien dan umur kultur. Penelitianini bertujuan menentukan pengaruh media dan umur panen terhadap kandungan fi kosianin dan aktivitasantioksidan. Kultivasi S. platensis dilakukan di dalam laboratorium menggunakan media KT, MT, dan Walne.Ekstraksi fi kosianin dilakukan menggunakan pelarut akuades dan bufer fosfat. Kandungan fi kosianin S.platensis dalam media MT (10,07 mg/mL) tidak berbeda nyata dengan media Walne (7,49 mg/mL), tetapiberbeda nyata dengan media KT (0,71 mg/mL). Aktivitas antioksidan S. platensis pada ketiga media tidakberbeda nyata. Kandungan fi kosianin S. platensis pada hari ke-12 (10,42 mg/mL) berbeda nyata dengan harike-6 (2,70 mg/mL), hari ke-14 (8,14 mg/mL), dan hari ke-17 (3,09 mg/mL). Aktivitas antioksidan S. platensispada keempat umur panen tidak berbeda nyata.Kata kunci: antioksidan, fi kosianin, Spirulina platensis, waktu panen
PENGARUH WAKTU PANEN DAN NUTRISI MEDIA TERHADAP BIOPIGMEN Spirulina platensis setyaningsih, Iriani -; Tarman, Kustiariyah -; satyantini, Woro hastuti; barus, dita aguistina
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 16 No. 3 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.596 KB) | DOI: 10.17844/jphpi.v16i3.8055

Abstract

Spirulina platensis merupakan alga hijau biru yang mengandung biopigmen fi kosianin dan antioksidanyang bermanfaat bagi kesehatan. Produksi biopigmen dipengaruhi oleh nutrien dan umur kultur. Penelitianini bertujuan menentukan pengaruh media dan umur panen terhadap kandungan fi kosianin dan aktivitasantioksidan. Kultivasi S. platensis dilakukan di dalam laboratorium menggunakan media KT, MT, dan Walne.Ekstraksi fi kosianin dilakukan menggunakan pelarut akuades dan bufer fosfat. Kandungan fi kosianin S.platensis dalam media MT (10,07 mg/mL) tidak berbeda nyata dengan media Walne (7,49 mg/mL), tetapiberbeda nyata dengan media KT (0,71 mg/mL). Aktivitas antioksidan S. platensis pada ketiga media tidakberbeda nyata. Kandungan fi kosianin S. platensis pada hari ke-12 (10,42 mg/mL) berbeda nyata dengan harike-6 (2,70 mg/mL), hari ke-14 (8,14 mg/mL), dan hari ke-17 (3,09 mg/mL). Aktivitas antioksidan S. platensispada keempat umur panen tidak berbeda nyata.Kata kunci: antioksidan, fi kosianin, Spirulina platensis, waktu panen
Growth of Nannochloropsis oculata Cultured using Tofu Liquid Waste Fertilizer Rahmadan, Rizky Yanuar; Satyantini, Woro Hastuti; Sari, Luthfiana Aprilianita
International Journal of Natural Science Studies and Development (IJOSS) Vol. 2 No. 1 (2025): IJOSS
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijoss.v2i1.18

Abstract

Growth of Nannochloropsis oculata is affected by nutrients in its culture media. Tofu liquid waste which is processed into fertilizer is expected to be an alternative nutrient that is more economical for microalgae culture activities. The purpose of this study was to determine the effect of tofu liquid waste fertilizer with different concentrations on the growth of Nannochloropsis oculata. This study used an experimental method with Completely Randomized Design (CRD). The treatment was different concentrations namely P1 (1 ml L-1), P2 (3 ml L-1), P3 (5 ml L-1), P4 (7 ml L-1) and P0 (Walne nutrient 1 ml L-1) each treatment were repeated four times. The main parameters in this study were cell density, cell growth rate and cell doubling time. Data obtained were analyzed using Analysis of Variance (ANOVA) and continued with Duncan's Multiple Range Test (DMRT). Results showed that the treatment of tofu liquid waste fertilizer with different concentrations had a significant different (p<0,05) on the growth of Nannochloropsis oculata. Concentration of 5 ml L-1 (P3) was the best concentration for the growth of Nannochloropsis oculata with peak cell density 1,335±13.54x104 cells ml-1, cell growth rate 0.900±0.001 cells ml-1 day-1 and cell doubling time 18.493±0.026 hours.
Subtitution Leaf Fermentation Leucaena (Leucaena leucocephala) on Feed vaname shrimp (Litopenaeus vannamei) for Retention of Protein and Energy Rheza Ariyandra; Agustono .; Woro Hastuti Satyantini
Journal of Aquaculture Science Vol 1 No 1 (2017): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v1i1.3

Abstract

Intensive aquaculture systems causing heavy reliance on the use of artificial feed (Heptarina et al., 2010). Feed is a crucial component to the success of shrimp farming (Soemardjati and Suriawan, 2006). According to Haryanto and Djajanegara (1993) lamtoro leaves contain protein, calcium and energy. The content of the leaves lamtoro a source of protein for feed ingredients in this plant are essential amino acids lysine and leucine were very high (D'Mello and Fraser, 1981). The consideration for the selection of feed materials should be done in accordance with the provisions of the feed material that is readily available, low cost, high nutrient content and do not compete with humans (Handajani et al., 2010). According to Matthew (1993), leucaena as forage quality has not been used optimally and not much commercialized. Lamtoro a potential biological resources to be used as feed to the waste generated sufficient forage nutritional value (Widiastuti, 2007). Research on the use of fermented flour substitution lamtoro leaf is a matter that needs to be done because of the use of fermented flour lamtoro leaf is expected to improve the quality of feed and make it as an alternative feed ingredient that can be given to increase aquaculture production. This research is the experimental method using a completely randomized design (CRD) with four treatments and five replications. The results of this study will be analyzed descriptively in the form of data and tables. Data obtained, processed using Analysis of Variance (ANOVA) to determine the effect of different treatments applied if real, then continued with Duncan's Multiple Range Test (Duncan's Multiple Range Test) with a 5% error rate to determine the best treatment.   Keywords: Feed, Vaname Shrimp, Fermentation, Substitution, Leucaena Leaf, Protein Retention, Retention Energy.  
Co-Authors A. Shofy Mubarak Abdul Manan Abdul Manan Agustono - Agustono . Agustono Agustono Agustono Agustono Akhmad Taufiq Mukti Alim Isnansetyo Alvira Febrianti Pratiwi Ananta Kurniawan Andry Wijayanto Anggun Nurani Citrowati Ariyandra, Rheza Asmaul Khusnah Aulia Ikhsan Syamsuri Ayu Puspitarani Boedi Setya Rahardja Brian Zuliyan Browijoyo Santanamurti Catur Pujiono Cicik Kurniawati, Cicik Daruti Dinda Nindarwi Dimas Eraparamarta Harkitiyanto dita aguistina barus Dita Wisudyawati Djamil, Hasim Dwitha Nirmala Emy Koestanti Sabdoningrum Enang Harris Falatehan, Naufal Febri Setyawati Firdaus, Fitrul Azizi Gunanti Mahasri Isroni, Wahyu Juanda Juanda Karina Rahardjo Kismiyati , Kustiariyah Tarman Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Lina Wafia Asmi Luthfiana Aprilianita Sari M Wahyu Alfian Meilina, Sinta Cahya Melani Indah Sari Manik Muhamad Amin Muhammad Amin Muhammad Arief Muhammad Izzudin Syaifullah Muhammed Jefri Myrna Budi N. Juni Triastuti Naila Budiatin Wahyu Mufidah Nina Nurmalia Dewi Nizar Afiansyah Loekman Nova Erika Nur Bambang Priyo Utomo Okky Hermawan Panhar, Fio Surya Pratama Semendo Prayogo Prayogo Pursetyo, Kustiawan tri Rachmat Rizaldi Rahayu Kusdarwati Rahmadan, Rizky Yanuar Rahmawati, Ghishella Ayu Rheza Ariyandra Rozi Rozi Sahidu, Adriana Monica Saiful Bakhri Saniya Lailatul Qodriyah Sapto Andriyono Saragih, Friska Setiawani Sari, Luthfiana Aprilanita Septuresty Hartri Eka Sihombing, Agusmanto Sudarno, Sudarno Sukenda . Vega Chrisnawati Veryl Hasan Vivaldy Phaza Muharta Yudi Cahyoko