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Journal : Journal of Tropical Plant Protection

Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (18.26 KB)

Abstract

Abstract Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan. Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus
Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Choliq, Fery Abdul; Chen, Tsang Hai; Sulistyowati, Liliek
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of19.5 kDa as the size of coat protein of tobamoviruses was estimated bysodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigidrod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp.in Taiwan. Keywords: Frangipani plant, mosaic disease, FrMV, Tobamovirus
Antifungal Effects of Plant Extracts on Colletotrichum gloeosporides in Chilli Pepper (Capsicum frutescens L.) Awanda Zidni Rizki; Fery Abdul Choliq; Mintarto Martosudiro
Journal of Tropical Plant Protection Vol 2, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.2.5

Abstract

Chili pepper (Capsicum frutescens L.) is a horticultural commodity that has high economic value and has the potential as an export commodity. One of the factors causing the decrease in productivity of chilli pepper is anthracnose caused by C. gloeosporioides. Consequently, plant-based fungicides might be served as eco-friendly alternatives to control C. gloeosporioides. This study aimed to invesitigated the effect of plant extracts from clove flowers, fennel seeds, basil, sweet basil, and citronella on growth inhibition of C. gloeosporioides in-vitro and in-vivo. Data were analyzed using Analysis of Variance (ANOVA). The five plant extracts were able to inhibit the growth of the pathogen C. gloeosporioides in vitro and in vivo. The highest inhibition of the pathogen C. gloeosporioides was basil extract treatment in-vitro compared to the control treatment. The in-vivo treatment, the lowest growth of C. gloeosporioides in fennel extract and was significanly different compared to the control treatment. 
Compatibility of Lemongrass Extract with Spodoptera litura Nuclear Polyhedrosis Virus Against Spodoptera litura on Soybean Plants Nafisatul Afidah; Tutung Hadiastono; Bedjo Bedjo; Fery Abdul Choliq
Journal of Tropical Plant Protection Vol 2, No 1 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.1.2

Abstract

Armyworm (Spodoptera litura) is an important pest that damages the leaves of soybean. SlNPV is one type of pathogen that has potential as a biocontrol agent to control armyworms because it is specific, selective, and effective. This study aimed to investigate the compatibility between the lemongrass and SlNPV in controlling armyworm. The Research was conducted at the Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from February 2016 until April 2016. The study using completely randomized design with 7 treatments and 3 replication with a treatment that consists of control, SlNPV-JTM 97C 1,5 × 106 PIB/ml, 10% of lemongrass extract, a combination SlNPV-JTM 97C fixed concentrate of 1,5 × 106 PIB/ml with 2.5%, 5%, 7.5%, and 10% of lemongrass extract. The results showed that the combination of SlNPV-JTM 97C 1.5 × 106 PIB/ml with 10% lemongrass had the highest level of virulence, with 96% of mortality on larva 100% on the pupa stage, so that the combination of SlNPV and lemongrass extracts was compatible in controlling S.litura. 
Effectiveness of Betel Leaf Extract Against Cercospora sp. Pathogen Causing Chili Leaf Spot In-Vitro Mahfud, Muhammad Afif; Martosudiro, Mintarto; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 5 No. 1 (2024)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2024.005.1.2

Abstract

The betel plant grows a lot and is easy to find in Indonesia. Part of the betel leaf contains antifungal compounds. This research examined the effect of betel leaf extract and its effective concentration in suppressing the pathogen Cercospora sp. in vitro. The research was conducted at the Pesticide Toxicology Laboratory, Universitas Brawijaya. Using a completely randomized design (CRD) with six treatments and five replications. The treatment was carried out in control and betel leaf extract with concentration levels of 30,000 ppm, 50,000 ppm, 100,000 ppm, 150,000 ppm, and 200,000 ppm. The results obtained were screening tests of compounds, causal tests of extracts on fungi, percentage of inhibition, and growth rate on fungi. Giving betel extract can suppress the growth of the fungus Cercospora sp., and in the 200,000 ppm extract treatment, the results were moderately effective in testing the percentage of inhibition power of the fungus Cercospora sp., which is equal to 41%.
The Effect of Giving Cherry (Muntingia calabura) Leaf Extract on Tobacco Mosaic Virus (TMV) Attacks on Cayenne Pepper Plants (Capsicum frutescens L.) Salsabilla, Alyara Oktavia; Martosudiro, Mintarto; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 5 No. 2 (2024)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2024.005.2.5

Abstract

Cayenne pepper (Capsicum frutescens L.) is a horticultural crop that is widely cultivated in Indonesia and has high economic value. However, it is not uncommon for the cultivation process to encounter several obstacles, one of which is caused by the Tobacco Mosaic Virus (TMV). One of the actions that can be taken to control this disease is to use induction agents derived from plant extracts. The plants that can be used as inducers are cherry plants (Muntingia calabura). However, research on the use of cherry leaf extract is rarely applied, especially against the TMV virus.  Thus, this research needs to be conducted to determine the effect of cherry leaf extract on TMV attack on cayenne pepper plants. The study used a completely randomized design (CRD) in vivo with 6 treatments and 4 replications. The treatment given was cherry leaf extract with different application frequencies at a concentration of 5%. Parameters observed included the disease incubation period, disease intensity, plant growth, and assessment of plant resistance. The results showed that applying cherry leaf extract with an application frequency of 4 times extended the incubation period of TMV and reduced the intensity of TMV disease, with values of 17.88 days after inoculation and 11.20%, respectively. This treatment resulted in a plant resistance category of "resistant." Additionally, the application of cherry leaf extract 4 times also had a positive effect on plant growth, specifically plant height and number of leaves.
RETRACTED : The Role of Trichoderma sp. as a Biocontrol Agent in Suppression of Root Rot Disease and Soybean Plant Biostimulants Maknunin, Lu'lu'il; Abadi, Abdul Latief; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 6 No. 1 (2025)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2025.006.1.2

Abstract

This article has been retracted at the request of the author. The author has published the article in the Jurnal Fitopatologi Indonesia (JFI).
Evaluation of Five Shallot (Allium Cepa L.) Varieties For Resistance to Shallot Yellow Stripe Virus (SYSV) Zulverdi, Yudhika Ankenkuala Ramadhana; Martosudiro, Mintarto; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 3 No. 1 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2022.003.1.3

Abstract

Shallot Yellow Stripe Virus (SYSV) is one of the economically important viruses of shallot. The incidence of SYSV infection can reach 66%-100%. The high level of SYSV infection on shallot is also due to the lack of resistance varieties to SYSV. This study aimed to determine the resistance of five varieties of shallot and the effect of SYSV infection on shallot production. The study used a completely randomized experimental design (CRD) using five shallot varieties as the treatment with four replication for each treatment. The shallot varieties were Bauji, Thailand, Bima Brebes, Tuk tuk (V4), Trisula (V5), and control. Each experimental unit consisted of 10 plants. The evaluation results showed that the Tuk-tuk variety was categorized as resistant, the Trisula variety as tolerant, and the Bauji, Thailand, and Bima Brebes varieties were categorized as susceptible. The shallot growth and production indicate that SYSV infection causes interference in the plant development process.
In Vitro Analysis of Effectiveness Clove Leaf Extract (Syzygium aromaticum) Against Anthracnose Disease (Colletotrichum capsici) on Chili Plants (Capsicum annum L) Mahendra, Syah Maulana; Abadi, Abdul Latief; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 3 No. 2 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2022.003.2.4

Abstract

Chili (Capsicum annum L) is a plant with a high economic value and is in great demand by the Indonesian people. This research aimed to determine the effectiveness of the difference in the concentration of clove leaf extract in inhibiting anthracnose disease in chili. This research was conducted from February to April 2022. This research used the Complete Randomized Design (CRD) method with six treatments and four replications. The treatment used in this research was the difference in the concentration of clove leaf extract, namely 0%, 1%, 2%, 3%, 4%, and 5%. The research showed that clove leaf extract could inhibit the growth of C. capsici, which caused anthracnose disease in chili. In vitro tests showed that the concentrations of 3%, 4%, and 5% of clove leaf extract significantly differed from all treatments and had fungicidal properties against C. capsici. In conclusion, the research results showed that the effective concentration in inhibiting the growth of C. capsici, which causes anthracnose disease in chili, is the concentration of clove leaf extract 3%, 4%, and 5%.
Effectiveness of Binahong (Anredera cordifolia) Leaf Extract in Suppressing The Growth of Xanthomonas oryzae and The Occurrence of Bacterial Leaf Blight Disease in Rice Plant (Oryza sativa) Khotimah, Dwi Kusnul; Abadi, Abdul Latief; Choliq, Fery Abdul
Journal of Tropical Plant Protection Vol. 4 No. 1 (2023)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2023.004.1.4

Abstract

Rice, as a staple food, is widely produced across Indonesia. However, bacterial leaf blight (BLB) in rice is caused by Xanthomonas oryzae pv. oryzae (Xoo) has been reported to cause significant crop yield losses. The continuous use of chemical bactericides to control BLB is not environmentally sustainable. This study investigated the potential of the Binahong plant as a botanical pesticide to suppress the growth of Xoo bacteria. Binahong is readily available in Indonesia and can be propagated generatively and vegetatively. The research employed a Completely Randomized Design (CRD) experimental method to evaluate the effectiveness of Binahong leaf extract against Xoo pathogens in vitro at various concentrations (0.5%, 1%, 1.5%, 2%) alongside positive and negative controls. In-vivo observations were made on several parameters: seed germination percentage, disease incidence, incubation period, and plant length. Data analysis revealed that Binahong leaf extract inhibited the growth of Xoo bacterial colonies in vitro at all tested concentrations (0.5% to 2%). Furthermore, soaking rice seeds in Binahong leaf extract was effective in vivo at all concentrations, demonstrating significant differences compared to the negative control and no significant differences compared to the positive control.