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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA Jurnal Ilmiah Profesi Pendidikan
Prapti Sedijani
Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Physics

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Isolation and Activity Test of Lipolitic Bacteria on Different pH and Temperature Prapti Sedijani; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati; Miko Eniarti; Siti Rohimah
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.4552

Abstract

Lipase is an enzyme that can be applied in many industrial fields so it has a good economic prospects. The demand for lipase was still unfulfilled at the time this article was written. The character of the lipase for industrial needs are vary depending on the industry in which the enzyme is used. Temperature and pH are the two factors that most influence enzyme activity. This article reports on bacteria isolated from coconut, avocado, tempeh, and areca nut and their lipolytic activity at pH 7-10, 30-40oC. Isolation was carried out on NA media supplemented with olive oil and Rhodamine B as the activity indicator. Colonies showing clear zones were used in subsequent work. Activity tests were carried out on the isolation medium pH 7-10 and incubated at 30 or 40oC. Characterization of isolates includes morphological and microscopic observations; proteolytic and amylolytic capability. There were 41 isolates showing lipolytic activity, 26, 7, 4, and 4 isolates from coconut, tempeh, avocado, and areca nut, respectively. Five isolates were selected for further work. Activity tests showed that the best isolates at 30oC were K1A1 and K1A2 at pH 7, each of which had an activity index of 9% and 107%. Meanwhile, at 40oC the best activity was shown by K10 pH 8 with an activity index of 59%. Based on Grams staining and microscopic analysis, isolates belong to Gram-positive coccus bacteria. Isolate K1A1 showed proteolytic and amylolytic activity, K1A2 showed high proteolytic activity but not amylolytic, while K10 did not show both activities.
Pengembangan Bahan Ajar Berbasis Next Generation Science Standard (NGSS) Terintegrasi Game Discovery untuk Melatih Literasi Sains dan Keterampilan Berargumentasi Ilmiah Lalu Hasan Nasirudin Zohri; Abdul Wahab Jufri; Prapti Sedijani; I Putu Artayasa; Abdul Syukur
Jurnal Ilmiah Profesi Pendidikan Vol. 7 No. 3b (2022): September
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jipp.v7i3b.784

Abstract

Literasi sains dan keterampilan berargumentasi ilmiah sangat krusial untuk diintegrasikan ke dalam pembelajaran di sekolah. Next Generation Science Standard dan game discovery dipandang kompatibel untuk melatih literasi sains dan keterampilan berargumentasi ilmiah dalam pembelajaran. Penelitian ini bertujuan untuk: 1) menganalisis tingkat kebutuhan pengembangan bahan ajar berbasis NGSS terintegrasi game discovery (BANDI); 2) merumuskan kerangka dasar BANDI; 3) menganalisis kelayakan BANDI. Jenis penelitian ini adalah research and development (R&D) dengan menggunakan model ADDIE. Subjek penelitian ini adalah kelas X IPA SMAN 01 Labuhan Haji. Teknik pengumpulan data melalui pengamatan langsung dan kuesioner. Analisis data menggunakan Content validity index (CVI), Percentage of agreement (PA), Flesch Kincaid grade level (FKGL), dan persentase nilai tanggapan. Hasil penelitian menunjukkan bahwa: 1) tingkat kebutuhan bandi berada pada kategori sangat dibutuhkan baik melalui respon siswa maupun guru, 2) Kerangka dasar BANDI terdiri atas 4 item (umum, basis, kompetensi, dan teknologi); 3) BANDI terkonfirmasi layak dikarenakan memenuhi aspek validitas, reliabilitas, keterbacaan, dan ketercapaian. Pemenuhan aspek kelayakan terlihat melalui hasil analisis data validitas BANDI melalui CVI dengan nilai rata-rata sebesar 0,96 dengan kategori valid, reliabilitas dianalisis melalui PA dengan nilai rata-rata sebesar 89,80% dengan kategori reliabel, keterbacaan sesuai dengan usia pengguna yaitu 15-16 tahun dengan nilai FKGL 10,69, serta keterterapan terkonfirmasi melalui uji kelompok kecil dengan nilai respon 89,39% untuk aspek kemudahan dan 84,52% untuk keefektifan penggunaan. Berdasarkan hasil yang diperoleh, maka dapat disimpulkan bahwa BANDI layak untuk selanjutnya digunakan dalam pembelajaran untuk memfasilitasi literasi sains dan keterapilan berargumentasi ilmiah di sekolah.
Fungal Crude Lipase Enzyme Produced Using the SSF (Solid State Fermentation) Method Increases The Washing Test Performance Prapti Sedijani; Nida'an Khovia; Dewa Ayu Citra Rasmi; Kusmiyati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6046

Abstract

Lipase is an enzyme that commonly used in various industries, including the detergent industry to improve washing performance. Two fungal isolates (Al and Cl) has been reported to be highly active on high pH. This research aims to see the potential of those fungi (crude enzyme) in improving washing performance. Enzymes were produced using the SSF (Solid State Fermentation) method with sugarcane bagasse as a medium. To determine how adding enzymes might affect the effectiveness of washing, studies were conducted. The results show that washing performance increases from 52% (detergent only) to 74% in combination with the crude enzyme. It is concluded that Al has a potential to be involved in biodetergent production. Similar assays with concentrated enzymes are suggested for future studies.
The Effect of Compost Based on Water Hyacinth and NPK Fertilizer on the Growth of Cayenne Pepper (Capsicum frutescens L.) Lale Agiet Safitri; Prapti Sedijani; Ahmad Raksun
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5492

Abstract

Continuous fertilization with inorganic fertilizers causes the soil to become hard and the balance of nutrients in the soil becomes disrupted so that the soil is unable to provide sufficient nutrients to support normal plant growth. This study aims to determine the effect of compost made from water hyacinth, NPK fertilizer, and their combinations on the growth of cayenne pepper plants, and to determine the best dosage of fertilizer on the growth response of cayenne pepper plants. This study used a completely randomized design (CRD) which consisted of 2 factors. Type of fertilizer and dose of fertilizer with each 4 levels of fertilization and 3 repetitions. The results of data analysis using the ANOVA test showed that the application of water hyacinth-based compost fertilizer; NPK fertilizer; and the combination can increase plant height, number of leaves, leaf area, fresh weight and dry weight on cayenne pepper plants. The DMRT test at the 5% level showed that the best treatment for plant height, wet weight and dry weight was a dose of 400 gr compost and 0 gr NPK, while the best treatment for leaf count was a dose of 200 gr compost and 0.4 gr NPK and the best treatment for Leaf area is a dose of 0 gr compost and 0.4 gr NPK.
The Effect of Egg Shell and Banana Peel Organic Fertilizer on The Growth Of Green Spinach (Amaratus tricolor L.) Nurseha Nurseha; Prapti Sedijani; Lalu Japa
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5505

Abstract

Long-term use of chemical fertilizers damages soil properties. Efforts to restore soil fertility by using liquid organic fertilizer are needed. Organic fertilizers from waste provide nutrition for plants while reducing environmental pollution. This study aims to analyze the effect of applying liquid organic fertilizer from banana peels, liquid organic fertilizer from egg shells, and the effect of the combination of liquid organic fertilizers. Two Way Anova test results with sig. a value <0.05 indicates that the POC of banana peel increased plant height, number of leaves, fresh weight and dry weight of green spinach. Eggshell POC increased wet weight and dry weight, while the combination of the two POC increased plant height and number of leaves, wet weight and dry weight of green spinach plants. This shows that the application of banana peel liquid organic fertilizer is more influential than the application of egg shell organic fertilizer.
Protease Enzyme Activity of Fungal Isolates from Avocado and Coconut Fleshes on Different pH and Temperature Prapti Sedijani; Dinda Propita Lestari; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5603

Abstract

Protease enzymes are widely applied in the industrial field. Protease enzymes have high economic prospects with unmet demand. This article describes protease activity of two fungi isolated from avocados and from coconuts fleshes that previously have shown a high lipolytic activity on pH 7 to pH 10.  The protease test was performed on PDA medium supplemented with 1% of skim milk, and adjusted to pH 7, pH 8 and pH 9 and was incubated at room temperature and at 30°C. Protease activity was determined based on the clear zone around the colony observed on day two – day eight. The results showed that variations in pH and temperature had a significant effect on the protease activity. The highest protease activity of isolate from avocado and from coconut fleshes were observed at pH 7, room temperature and incubated for 24 hours, with a protease activity index of 0.36 and 0.31 respectively.
Isolation and Screening of Amilolytic Bacteria Isolate from Cassava (Manihot utilissima) Prapti Sedijani; Bq. Novi Aprilia; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5604

Abstract

Amylase is an enzyme capable of hydrolyzing starch into simpler sugars. The use of amylase enzymes is urgently needed in various industrial fields, therefore the search for sources of amylase enzyme producers continues to be carried out for sources of amylase enzyme producers. Each industrial field has specific requirements for amylase enzymes. This study aims to isolate bacteria capable of producing amylase enzyme and to determine the amylase activity of bacteria isolated from cassava (Manihot utilissima) when incubated at different temperatures (room temperature and 30℃) and pH levels (7, 8, 9, and 10). The research involved bacterial isolation, testing the amylase activity of the isolated amylolytic bacteria at varying pH and temperatures, and characterizing the amylolytic bacteria. The amylolytic bacterial activity was qualitatively measured based on the clear zone's area formed around the colony. From this study 5 isolates showing amylolytic activity were obtained. The amylolytic activity index varied among the isolates, ranging from 0.12 to 0.59. The highest amylolytic activity index (0.59) was performed by S4 isolate on pH 10 medium incubated at 30℃. Microscopic and gram staining analysis suggest that two isolates were gram-negative coccus bacteria and three of them were gram-positive coccobaccilus bacteria.
Isolation and Molecular Characterization of Brotowali (Tinospora crispa) Rhizosphere Bacteria Producing Siderophore from Dry Lands of Lombok Island Sekar Partiwi; Agil Al Idrus; Lalu Zulkifli; Mahrus; Prapti Sedijani
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6138

Abstract

In the context of biopesticide development, siderophore-producing rhizosphere bacteria play an important role as potential biological control agents. Siderophores are complex organic compounds produced by microorganisms to bind and take up iron ions (Fe3+) from the surrounding environment. This compound is very important because iron is a nutrient necessary for life for many microorganisms and plants. The aim of this research was to determine the type and ability of bacteria in the rhizosphere of Brotowali (Tinospora crispa) to produce siderophores. The method used in this research is an exploratory descriptive type. The isolation results obtained from the North Lombok area obtained 5 samples with codes B4A1, B6A1, BT61, BT63 and BT83. The results of the bacterial isolate potency test with Media Kings showed that only isolate BT63 showed clear fluorescence when exposed to ultraviolet light. Meanwhile, the Arnow's Assay test showed that only BT63 colonies experienced a color change to pink. The results of the molecular identification of the potential bacteria, namely BT63, using the BLAST method using the GenBank data base at NCBI, show that it is closely related to the species Bacillus thuringiensis strain FDAARGOS 791. The results above show that the rhizobacteria isolated from Brotowali have the potential to be developed as a natural biopesticide that can be applied in the future.
Co-Authors Abdul Syukur Abdul Wahab Jufri Agil Al Idrus Agus Ramdani Ahmad Raksun Angelina Putri Ayu Lestari Baiq Elita Mandalini Bq. Novi Aprilia Dadi Setiadi Dewa Ayu Citra Rasmi Dewa Ayu Citra Rasmi Dinda Propita Lestari Dwi Soelistya Dyah Jekti I Gde Mertha Imam Bachtiar Kusmiyati Kusmiyati Lale Agiet Safitri Lalu Hasan Nasirudin Zohri Lalu Japa Lalu Zulkifli Mahrus Mahrus Mahrus Miko Eniarti Miko Erniarti Ni Luh Putu Pebri Artayani . Nida'an Khovia Nurseha Nurseha Nurul Fadhilah Priyo Hartanto Putri Alfianti Sriwinahyu Rustam - Sekar Partiwi Siti Rohimah Syamsul Bahri