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Deteksi Mekanisme Efflux Pump Pada Resistensi Bakteri Staphylococcus haemolyticus, Sampel Pus Klinis Rumah Sakit Pekanbaru Wardana, Rexi Dwi; Savira, Maya; Anggraini, Dewi; Amalia, Aisyah Sabrina; Nurulita, Yuana
Chimica et Natura Acta Vol 12, No 3 (2024)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v12.n3.52038

Infeksi adalah masalah kesehatan yang disebabkan oleh mikroorganisme patogen seperti bakteri. Salah satu jenis infeksi yaitu infeksi kulit dan jaringan lunak yang umumnya disebabkan oleh Staphylococcus aureus dan menyebabkan pembentukan pus. Saat ini, telah dilaporkan bahwa kasus spesies CoNS (Coagulase Negative Staphylococcus) seperti Staphylococcus haemolyticus sebagai penyebab infeksi, mengalami peningkatan. Selain itu, spesies ini juga diketahui telah mengalami resistensi terhadap beberapa golongan antibiotik. Dalam penelitian ini dilakukan karakterisasi isolat bakteri klinis dari sampel pus agar yang dilakukan dengan identifikasi mikrobiologi (kultur isolat, pewarnaan Gram dan uji biokimia) pada bakteri patogen (Isolat 3887), dan dilanjutkan dengan identifikasi molekuler, antara lain ekstraksi DNA kromoson dan plasmid, amplifikasi gen 16S rRNA, sekuensing dan analisis bioinformatik menggunakan BioEdit, BLAST dan filogenetika. Uji suseptibilitas terhadap 25 jenis antibiotik dan deteksi gen resisten clindamycin (ermA, ermB, ermC dan msrA) dan cefazolin (blaTEM-1, blaSHV dan blaOXA-1) dilakukan untuk mengidentifikasi kemungkinan mekanisme resistensi bakteri patogen. Berdasarkan hasil identifikasi mikrobiologi dan molekuler, isolat 3887 merupakan CoNS dengan strain Staphylococcus haemolyticus. Isolat 3887 resisten terhadap 12 dari 25 antibiotik yang diuji dan memiliki fenotipe resisten tertinggi terhadap clindamycin dan cefazolin. Hasil uji deteksi gen resisten clindamycin dan cefazolin menunjukkan hanya gen msrA yang terdeteksi baik pada kromosom dan plasmid isolat 3887 yang menunjukkan bahwa mekanisme resistensi melalui efflux pump.

Promising candidate drug target genes for repurposing in cervical cancer: A bioinformatics-based approach Pratiwi, Nurfi; Ulfah, Aida J.; Rachmadina, Rachmadina; Irham, Lalu M.; Afief, Arief R.; Adikusuma, Wirawan; Darmawi, Darmawi; Kemal, Rahmat A.; Rangkuti, Ina F.; Savira, Maya
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.938

Cervical cancer is the fourth most common cancer among women globally, and studies have shown that genetic variants play a significant role in its development. A variety of germline and somatic mutations are associated with cervical cancer. However, genomic data derived from these mutations have not been extensively utilized for the development of repurposed drugs for cervical cancer. The objective of this study was to identify novel potential drugs that could be repurposed for cervical cancer treatment through a bioinformatics approach. A comprehensive genomic and bioinformatics database integration strategy was employed to identify potential drug target genes for cervical cancer. Using the GWAS and PheWAS databases, a total of 232 genes associated with cervical cancer were identified. These pharmacological target genes were further refined by applying a biological threshold of six functional annotations. The drug target genes were then cross-referenced with cancer treatment candidates using the DrugBank database. Among the identified genes, LTA, TNFRSF1A, PRKCZ, PDE4B, and PARP were highlighted as promising targets for repurposed drugs. Notably, these five target genes overlapped with 12 drugs that could potentially be repurposed for cervical cancer treatment. Among these, talazoparib, a potent PARP inhibitor, emerged as a particularly promising candidate. Talazoparib is currently being investigated for safety and tolerability in other cancers but has not yet been studied in the context of cervical cancer. Further clinical trials are necessary to validate this finding and explore its potential as a repurposed drug for cervical cancer.

Studi Awal Analisis Molekuler Human Papillomavirus dari Apusan Glans dan Batang Penis Savira, Maya; Yuwandari, Resty; Maryanti, Yossi; Kemal, Rahmat Azhari; S, Donel
Jurnal Kesehatan Andalas Vol. 9 No. 4 (2020): Online December 2020
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1493

Pria juga dapat mengalami keganasan akibat infeksi Human Papillomavirus (HPV) serta bertindak sebagai reservoir virus. Metode skrining HPV pada wanita telah terstandardisasi, namun belum ada standar metode skrining pada pria di Indonesia. Beberapa studi pada populasi pria di luar negeri menunjukkan potensi sampling pada daerah genitalia eksterna untuk skrining HPV. Tujuan: mengoptimasi metode skrining HPV secara molekuler pada pria. Metode: Responden adalah partner seksual wanita pansien kanker serviks di RSUD Arifin Achmad Provinsi Riau. Apusan dari glans dan batang penis diambil menggunakan nylon-flocked swab yang kemudian dimasukkan ke dalam 350Âµl viral transport medium terpisah. DNA diisolasi dari sampel yang kemudian dianalisis untuk mendeteksi gen human Î²-globin dan HPV. Hasil: Optimasi awal menunjukkan gen Î²-globin dapat terdeteksi dari hasil ekstraksi dengan kit Zeesan Viral RNA Extraction. Pita HPV hasil PCR dengan primer MY09 dan MY11 dapat muncul namun masih tipis. Simpulan: Studi awal ini menunjukkan bahwa apusan glans dan batang penis dapat digunakan untuk deteksi HPV secara molekuler pada pria, namun proses pengambilan sampel, ekstraksi DNA, dan PCR masih perlu dioptimasi.Kata kunci: apusan, glans, HPV, penis

Genetic variations of the L2 gene in human papillomavirus (HPV) type 16 from cervical cancer patients in Sumatra region, Indonesia Savira, Maya; Farniga, Arnaldi; Ilmiah, Zidny; Rachmadina, Rachmadina; Rini, Ika A.; Kemal, Rahmat A.; Mahargyarani, Azza E.; Admiral, Muhammad Z.; Sofian, Amru; Razali, Renardy R.; Suhaimi, Donel; Putra, Andani E.
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1653

The L2 protein, a minor capsid component of human papillomavirus (HPV), plays a critical role in the HPV life cycle by packaging the viral genome with the L1 protein and facilitating DNA transport to the nucleus. Identifying genetic variations in the L2 gene is essential for improving vaccine development, diagnostic accuracy, and understanding viral evolution, potentially contributing to more effective HPV vaccines. The aim of this study was to investigate the genetic variation of the L2 gene in cervical cancer specimens collected from patients in Riau Province, Indonesia. A single-center, cross-sectional study was conducted at Arifin Achmad General Hospital, Riau Province, involving cervical cancer patients with confirmed HPV16 infection between January 2018 and August 2020. Demographic, clinical, and risk factor data were collected through structured interviews and direct assessments. Cervical biopsy specimens were collected, and viral DNA was extracted for L2 gene amplification using polymerase chain reaction (PCR). Sequencing was conducted on PCR products, followed by single-nucleotide polymorphism (SNP) identification through alignment with the HPV16 reference genome. The amplification and sequencing of the HPV16 L2 gene from 22 cervical cancer specimens revealed 36 SNPs, including 31 nonsynonymous and five synonymous mutations. High-frequency mutations were observed at nucleotide positions 4,074 and 4,177, each detected in 95.45% of the samples. Notable insertions were found at positions 3,668–3,669 and 4,275–4,276, indicating substantial sequence variation. Phylogenetic analysis grouped the sequences into three clusters, with most belonging to sub-lineage A2 (European), while others aligned with A4 (Asian) and East Asian lineages. The observed genetic diversity in the HPV16 L2 gene may reflect regional viral evolution and has potential implications for future vaccine development.

Adjunctive Vaginal Probiotic Therapy for Preterm Premature Rupture of Membranes: A Systematic Review and Meta-Analysis of Latency Period, Maternal Infection, and Neonatal Morbidity Vani Ardiani; Donel S; Maya Savira; Zulmaeta
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 1 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i1.1484

Background: Preterm premature rupture of membranes (PPROM) significantly drives preterm birth rates and consequent neonatal morbidity and mortality. While standard antibiotic therapy aims to prolong pregnancy latency, it concurrently disrupts the protective vaginal microbiota. Adjunctive vaginal probiotics have been investigated as a means to restore beneficial flora, potentially mitigating ascending infection and improving perinatal outcomes. This study systematically synthesized the current randomized trial evidence regarding this adjunctive therapeutic strategy. Methods: We performed a systematic review and meta-analysis following PRISMA guidelines. PubMed, EMBASE, and CENTRAL databases were searched (2014–October 2025) for randomized controlled trials (RCTs) comparing adjunctive vaginal probiotics plus antibiotics versus antibiotics (alone or with placebo) in singleton pregnancies complicated by PPROM between 24+0 and 34+0 weeks’ gestation. Primary outcomes included the latency period (days) and maternal chorioamnionitis or infectious morbidity. Key secondary outcomes were neonatal intensive care unit (NICU) admission, neonatal sepsis, and neonatal mortality. Data were pooled using a random-effects model, calculating Mean Differences (MD) or Risk Ratios (RR) with 95% Confidence Intervals (CI). Risk of bias was assessed using the Cochrane RoB 2 tool. Results: Three RCTs, encompassing 330 participants, met the inclusion criteria. Significant methodological limitations, including high risk of bias and critical baseline confounding by gestational age in the largest trial, were identified across the included studies. A sensitivity analysis addressing high heterogeneity (I²=98%) for latency (excluding one retrospective study; n=290) indicated a modest but statistically significant prolongation associated with probiotics (MD 2.98 days; 95% CI 1.80–4.16; p<0.0001; I²=0%). Probiotic use was linked to a significantly lower risk of maternal infection (RR 0.43; 95% CI 0.24–0.77; p=0.005; I²=0%; n=270). Statistically significant reductions were also observed for NICU admission (RR 0.59; 95% CI 0.46–0.75; p<0.0001; I²=55%; n=330) and neonatal mortality (RR 0.38; 95% CI 0.18–0.81; p=0.01; I²=0%; n=270), although these estimates are likely inflated due to baseline confounding. Conclusion: This meta-analysis suggests adjunctive vaginal probiotics may offer benefits in PPROM management by modestly prolonging latency and significantly reducing maternal infectious morbidity. While substantial reductions in NICU admission and neonatal mortality were observed, these findings must be interpreted with extreme caution due to the limited quantity and low quality of the primary evidence, particularly the high risk of bias and confounding. Definitive conclusions cannot be drawn, and routine clinical adoption is not supported by current evidence. High-quality, large-scale RCTs are imperative.

Health Nutrition and Sanitation Counseling Education in Improving Health Understanding in Sigapiton Village, Ajibata District, North Sumatra. Rusdiana; Lubis, Nenni Dwi Aprianti; Savira, Maya; Widjaja, Sry Suryani
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2021): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Healthy environmental sanitation and good nutrition are factors that determine human resources and quality of life. With healthy environmental sanitation, the community is protected from various infectious diseases and similarly with a good understanding of nutrition will produce individuals with good quality. For this reason, it is important to achieve resources and good quality of life. This understanding is enhanced by providing health education. and good nutrition so that people are protected from various diseases. Health matters are matters of the environment, attitudes and behavior of the community. One of the causes of this nutritional deficiency is stunting, namely short stature in which a child who is stunted produces a low level of intelligence. For this reason, it is necessary to educate the public to understand the importance of good nutrition for children's growth. There is a wrong perception in the general public that stature is a hereditary factor, not a factor in malnutrition so that they do not pay attention to the nutritional status of children. Wrong choice of food for consumption, as well as nutritional balance between intake and output of substances (nutritional imbalance), namely intake that exceeds output or vice versa is the result of nutritional problems that occur in toddlers. This causes several impacts, including chronic disease, overweight and underweight, pica, dental caries, and allergies. For this reason, in this case the community service team wants to provide education about counseling to the community where the targets to be carried out are pregnant women, school children and young women. During the Covid 19 pandemic, the targets we educate are through cadres and village officials, this is to avoid infection with the covid 19 virus. By providing counseling to cadres and village officials, we hope that it will reach the people of Sigapiton village. Where in this counseling we provide pocket books to the cadres and village officials that we educate. We hope that this service can increase public knowledge about nutrition understanding and the creation of healthy environmental sanitation. We will publish the output of this service in an international journal indexed by Scopus

Comparison Ghrehlin at Obesity Without Metabolic Sydrome With Obesity with Metabolic Syndrome Rusdiana; Savira, Maya; Widjaja, Sry Suryani
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 1 (2021): ABDIMAS TALENTA : Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Obesity is principal causative factor in the development of metabolic syndrome and Ghrelin as human natural hormones is involved in fundamental regulatory processof eating and energy balance.Obesity, which has become a global public health problem, is one of the major risk factors for development metabolic syndrome and type 2 diabetes mellitus.This study aimed to analyze the comparison of ghrelin hormone levels in obese with metabolic syndrome and obese non metabolic syndrome . The sample population is obese adults, then we examined the weight, height, waist size, blood pressure, laboratory tests such as blood sugar levels and lipid profile of sample population to separate obese with metabolic syndrome and obese non metabolic syndrome. After we determined each group we measured stress oxidative levels in blood in obese with metabolic syndrome and obese non metabolic syndrome by ELISA method.With statistical analysis using T test found that there was significant difference of ghrelin hormone levels between obese with metabolic syndrome and obese without metabolic syndrome (p<0.005).

Adjunctive Vaginal Probiotic Therapy for Preterm Premature Rupture of Membranes: A Systematic Review and Meta-Analysis of Latency Period, Maternal Infection, and Neonatal Morbidity Vani Ardiani; Donel S; Maya Savira; Zulmaeta
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 1 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i1.1484

Background: Preterm premature rupture of membranes (PPROM) significantly drives preterm birth rates and consequent neonatal morbidity and mortality. While standard antibiotic therapy aims to prolong pregnancy latency, it concurrently disrupts the protective vaginal microbiota. Adjunctive vaginal probiotics have been investigated as a means to restore beneficial flora, potentially mitigating ascending infection and improving perinatal outcomes. This study systematically synthesized the current randomized trial evidence regarding this adjunctive therapeutic strategy. Methods: We performed a systematic review and meta-analysis following PRISMA guidelines. PubMed, EMBASE, and CENTRAL databases were searched (2014–October 2025) for randomized controlled trials (RCTs) comparing adjunctive vaginal probiotics plus antibiotics versus antibiotics (alone or with placebo) in singleton pregnancies complicated by PPROM between 24+0 and 34+0 weeks’ gestation. Primary outcomes included the latency period (days) and maternal chorioamnionitis or infectious morbidity. Key secondary outcomes were neonatal intensive care unit (NICU) admission, neonatal sepsis, and neonatal mortality. Data were pooled using a random-effects model, calculating Mean Differences (MD) or Risk Ratios (RR) with 95% Confidence Intervals (CI). Risk of bias was assessed using the Cochrane RoB 2 tool. Results: Three RCTs, encompassing 330 participants, met the inclusion criteria. Significant methodological limitations, including high risk of bias and critical baseline confounding by gestational age in the largest trial, were identified across the included studies. A sensitivity analysis addressing high heterogeneity (I²=98%) for latency (excluding one retrospective study; n=290) indicated a modest but statistically significant prolongation associated with probiotics (MD 2.98 days; 95% CI 1.80–4.16; p<0.0001; I²=0%). Probiotic use was linked to a significantly lower risk of maternal infection (RR 0.43; 95% CI 0.24–0.77; p=0.005; I²=0%; n=270). Statistically significant reductions were also observed for NICU admission (RR 0.59; 95% CI 0.46–0.75; p<0.0001; I²=55%; n=330) and neonatal mortality (RR 0.38; 95% CI 0.18–0.81; p=0.01; I²=0%; n=270), although these estimates are likely inflated due to baseline confounding. Conclusion: This meta-analysis suggests adjunctive vaginal probiotics may offer benefits in PPROM management by modestly prolonging latency and significantly reducing maternal infectious morbidity. While substantial reductions in NICU admission and neonatal mortality were observed, these findings must be interpreted with extreme caution due to the limited quantity and low quality of the primary evidence, particularly the high risk of bias and confounding. Definitive conclusions cannot be drawn, and routine clinical adoption is not supported by current evidence. High-quality, large-scale RCTs are imperative.

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