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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems Jurnal Ilmu Dasar Microbiology Indonesia BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication UNEJ e-Proceeding Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Riset Biologi dan Aplikasinya Life Science and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Kartika Senjarini
Biology Department, Faculty Of Mathematic And Natural Sciences, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

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Journal : Global Medical and Health Communication

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Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi Evi Umayah Ulfa; Esti Utarti; Izzay Afkarina; Sattya Arimurti; Kartika Senjarini
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.707 KB) | DOI: 10.29313/gmhc.v5i2.1914

Abstract

Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is  capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.
Co-Authors Adrial Adrial Agustin, Dita Paramytha Ahmad Tosin Ainiyah, Durotun Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Ali Machrus Anak Agung Gede Sugianthara Anam, Khairul Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Arif Setiawan Azkiyah, Siti Fat'hiyatul Bagus Setiawan Berlian Permata Dewi Erlambang Budayatin, Budayatin Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Didik SULISTYANTO DINA FITRIYAH Dita Paramytha Agustin Esti Utarti Evi Umayah Ulfa Febriyantiningsih, Dwi Esti Fitria Mutiah Fitria Muti’ah Hidayat Teguh Wiyono Husnul Hotimah, Husnul Iflaha, Adriana Ihsanul Azmi Intan Fitri Indrasari Izzay Afkarina Kahar Muzakhar Khasanah, Rochmatul Nuryu Labes, Antje Laili, Alvina Nur Lailly Nur Uswatul Hasanah Lelono, Asmoro Loeki Enggar Fitri Mahriani Mahriani MAsruroh, Binti Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nuril Azizah Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Ratis Nour Sholichah Rehmann, Holger Renam Putra Arifianto Rhena Schumann Riana Agatha Listiani Rike Oktarianti Saraswati, Dyah Rahmadhani Sattya Arimurti Septiawan, Mahful Silvya Fitri Nur Azizah Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sutoyo Sutoyo Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Teguh Wahju Sardjono Teguh Wahju Sardjono Tri Yudani MR Ulf Karsten Utami, Diah Ayu Wathon, Syubanul Wathon, Syubbanul - Widya Cahyadi Widyadana, Kaysha Rahmadita Yasir Mubarok Yenny Febriana Ramadhan Abdi Yunita Armiyanti Zahniar, Zahniar