Claim Missing Document
Check
Articles

Found 16 Documents
Search

Extraction and Partial Characterization of Lectin from Indonesian Brown Algae Padina australis and Padina minor Nurrahmi Dewi Fajarningsih; Naomi Intaqta; Danar Praseptiangga; Choiroel Anam
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i3.400

Abstract

Extraction and partial characterization of lectin from Indonesian Padina australis and Padina minor had been carried out. The crude extract of the P. australis and P. minor were examined for hemagglutination activity (HA) using native and trypsin-treated of rabbit and human A, B, O type erythrocytes. Both extracts agglutinated all of the trypsin-treated erythrocytes tested in the HA assay. Strong HA was detected in the crude extract of P. minor with trypsin-treated of human type A and O erythrocytes. However, the sugar-binding specificity study through the quantitative hemagglutination inhibition (HI) assay showed that P. minor extract could not specifically recognize the glycans tested. Apparently, the HA of the P. minor was more due to its co-extracted polyphenols content than its lectin content. On the other hand, the HI assay showed that asialo transferrin human (aTf) and asialo porcine thyroglobulin (aPTG) were the most powerful in inhibiting the HA of P. australis. Those indicated that P. australis protein extract was able to specifically recognized aTf and aPTG. The stability of P. australis and P. minor HA over various temperatures, pH ranges, and divalent cations studies showed that the P. minor HA was stable on a wide range of pH and temperature; not affected by the presence of EDTA, but decreased by Ca2+ and Mg2+ additions showed that P. minor protein extract  was not a metallic protein. The HA of P. australis decreased at 60 oC and was inactivated at 90 oC; increased at strong acidic (pH 3 4) and strong basic (pH 9 10) and dependent by the presence of either EDTA or Ca2+ and Mg2+ divalent cation.
PENAPISAN HEMAGGLUTININ DARI ALGA HIJAU GENUS Codium (Chlorophyceae, Codiaceae) Danar Praseptiangga
Jurnal Teknologi Hasil Pertanian Vol 6, No 1 (2013)
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.008 KB) | DOI: 10.20961/jthp.v0i0.13234

Abstract

Lektin (hemagglutinin) tersebar secara luas di alam dan memiliki potensi yang baik terkait denganprospeksinya dari alga laut. Sifat terpenting lektin adalah kemampuannya untuk mengikat karbohidrat danmengaglutinasi sel. Lektin dapat berguna sebagai alat yang mudah digunakan untuk menunjukkan perbedaanstruktur karbohidrat dan mempunyai berbagai macam aktifitas biologis terkait dengan pengikatan daninteraksinya dengan karbohidrat, sehingga terdapat ketertarikan yang besar dalam upaya untuk menemukan danmengisolasi lektin baru dari perspektif penggunaan lektin, khususnya dalam bidang glycomics dan medis. Fraksikasar lektin dari enam spesies alga hijau genus Codium disiapkan dan diuji aktifitas hemaglutinasinya denganmenggunakan trypsin-treated rabbit erythrocytes. Hasil positif pada limaspesies alga hijau genus Codium yangdiuji dalam hemagglutination assay mengindikasikan keberadaan lektin (hemagglutinin) pada spesies-spesiestersebut. Namun demikian, masih diperlukan penelitian lebih lanjut mengenai pemurnian lektin dan karakterisasisifat biokimianya.
A Teknologi Mesin Pengasap Telur Asin Herbal sebagai Strategi Memperluas Market Share Anam, Choiroel; Praseptiangga, Danar; Ridwan Ariyantoro, Achmad; Yulviatun, Anastriyani; Okta Aldayra, Fannia; Wardhani, Fannisa
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 9 No 1 (2024): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Trend konsumsi telur dan potensi telur asin di Indonesia maupun mancanegara terus meningkat terlihat dari permintaan pasar yang semakin banyak, baik untuk konsumsi ke konsumen langsung, untuk oleh-oleh, hantaran pernikahan atau acara keagamaan, untuk bekal umroh/haji, dan juga saat travelling ke berbagai negara, apalagi telur asin terjamin kehalalannya. Dalam memenuhi permintaan konsumen diperlukan inovasi produk dan inovasi teknologi tepat guna supaya produk tetap memiliki kualitas mutu yang baik. Metode yang digunakan untuk mencapai tujuan tersebut yaitu melalui pemenuhan aspek legalitas produk, aspek produksi, aspek kemasan dan penampilan, serta melakukan pendampingan. Aplikasi teknologi yang diterapkan dalam produksi telur asin rempah asap herbal adalah alat teknologi tepat guna pengasap telur. Dari kegiatan ini mitra dapat memiliki legalitas usaha, produk yang memenuhi standar sesuai persyaratan yang telah ditentukan serta penampilan produk yang eye catching dan marketable dengan masa simpan yang lebih panjang dibandingkan telur asin biasa.
PENURUNAN DERAJAT KEASAMAN SALIVA PADA ANAK USIA 10-12 TAHUN YANG MENGKONSUMSI COKELAT HITAM SUKROSA, XYLITOL, DAN STEVIA Kaswindiarti, Septriyani; Saputra, Muhammad Rhaufly Dwi Arga; Yudiatna, Clara Cessa Putri; Widianto, Shafwan Rafif; Muhammad, Dimas Rahadian Aji; Praseptiangga, Danar; Murdiyanto, Dendy; Sari, Nendika Dyah Ayu Murika; Mardiyati, Nur Lathifah
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Vol 11, No 2 (2024): Vol. 11,No 2 (Desember 2024)
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/jbd.v11i2.1649

Abstract

Introduction: Chocolate is a widely consumed food, particularly by children. It is often considered a contributing factor to the development of dental caries due to its high sugar content, which can lead to the formation of dental caries. Aim: This study aimed to determine whether consuming dark chocolate sweetened with sucrose, xylitol, and stevia would reduce saliva acidity in children aged 10-12 years. Methods: Twenty-one children aged 10-12 years were given dark chocolate with three sweeteners: 30% sucrose, 30% xylitol, and 1% stevia. The subjects' saliva was collected 10 minutes after chewing chocolate with sucrose on day 1, chocolate with xylitol on day 2, and chocolate with stevia on day 3 for 30 seconds and then measured by a pH meter. The measurement was repeated thrice. Result: The difference in mean saliva pH before and after treatment with the three types of chocolate were analysed using the One-way ANOVA test.  A decline in pH was observed in all treatment groups; however, the most significant decrease was observed in the sucrose chocolate group (0.34 ± 0.12), followed by the stevia chocolate group (0.26 ± 0.09), and the least significant decrease was observed in the xylitol chocolate group (0.14 ± 0.07). Conclusion: A statistically significant difference was observed in the decrease in saliva acidity among children aged 10-12 years who consumed dark chocolate containing sucrose, xylitol, and stevia
Permainan Iza! Kaeru Caravan Sebagai Media Pembelajaran Mitigasi Bencana Dalam Pengembangan Sosial Emosional Anak Usia Dini di Kelurahan Sumber, Banjarsari, Surakarta Handayani, Kusumaningdyah Nurul; Purwandaru, Pandu; Praseptiangga, Danar; Mutiah, Inas Hasnah
Buletin Dharmas Andalas Vol. 2 No. 2 (2025): Buletin Dharmas Andalas
Publisher : Departemen Budidaya Tanaman Perkebunan, Fakultas Pertanian, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bda.v2i2.15

Abstract

Surakarta adalah salah satu kota yang terletak di Provinsi Jawa Tengah. Kelurahan Sumber diklasifikasikan sebagai tingkat ancaman bencana banjir karena berada di dekat tiga sungai. Pentingnya advokasi mengenai banjir tidak bisa dianggap remeh karena banjir dapat menyebabkan kerugian besar terhadap kehidupan masyarakat setempat. Tujuan penelitian ini adalah untuk menganalisis kemampuan sosial emosional anak usia 4-12 tahun di Kelurahan Sumber melalui Permainan Iza! Kaeru Caravan. Dalam penelitian ini, digunakan pendekatan kualitatif jenis studi kasus. Dengan menggunakan pendekatan ini, diharapkan dapat memperoleh pemahaman yang lebih mendalam mengenai kemampuan sosial emosional. Faktor yang memiliki jumlah poin terbesar adalah faktor dengan pengaruh terbesar. Sedangkan faktor dengan jumlah poin terkecil memiliki pengaruh paling kecil. Variabel kemampuan sosial emosional yang sering terjadi pada anak usia 4-6 tahun berdasarkan perhitungan menggunakan metode borda di Kelurahan Sumber yaitu variabel menunjukkan sikap mandiri dalam memilih kegiatan dengan faktor pendidikan dan dukungan orang tua. Sedangkan pada anak usia 7-9 tahun dan anak usia 10-12 tahun yaitu variabel menunjukkan sikap mandiri dalam memilih kegiatan dengan faktor minat dan bakat.
Research Trends on the Use of Avocado Starch for Biofilm Production: Bibliometric Analysis Using VOSviewer Nazir, Novizar; Hariry, Amelia; Widiyastuti, W.; Panatarani , Camelia; Praseptiangga, Danar; Aziz, Rizki; Syamani, Firda Aulya
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 3 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v7i3.359

Abstract

Avocado starch has gained attention as a potential bio-based material due to its unique features that make it suitable for forming biofilms. This work thoroughly examines the changing field of research focused on using avocado starch for biofilm applications. It uses VOSviewer, a tool for analyzing bibliometric data, to uncover important insights using a database of Scopus-indexed journal publishers. This study aimed to determine research trends on the use of avocado starch for biofilm production. The Scopus database collected information on research trends in the use of avocado starch for biofilm production. To obtain search results, subject categories with titles, keywords, and abstract criteria from the use of avocado starch extraction, biofilm/bioplastic production, and biocomposite were used as a reference. Using VOSviewer, search result extraction was performed. The results of bibliometric mapping were then further evaluated. The findings of the bibliometric study are demonstrated through network visualization, overlay visualization, and density visualization. The findings indicate that starch produced from avocados has the potential to be a viable resource for manufacturing biofilms. However, further investigation is required to enhance the overall quality of the produced biofilm.