I Ketut Suatha
Laboratorium Anatomi Hewan,,Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Journal : INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES

CENTELLA ASIATICA EXTRACT INCREASED ON THE LEVEL OF INTERLEUKIN 6 (IL-6) IN MICE Kerta Besung, I Nengah; Mantik Astawa, Nyoman; Suatha, I Ketut; -, Hartaningsih
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 1 Januari 2011
Publisher : Udayana University

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Abstract

Salmonellosis is still problem in many developing countries including Indonesia. Themain problem in controlling and handling the disease is that only few antibiotics are availableto cure the disease. In addition, the prolonged use of such antibiotics often lead to bacterialresistant against the antibiotics. A herbal drugs such as Centella asiatica (in Indonesia isknown as pegagan) contains triterphenoid saphonins which acts as immunostimulant capableof enhancing the phagocytic activity of macrophages. However, no study has been conductedto investigate the use pegagan in activating macrophage of mice infected with Salmonellatyphi. A study was therefore conducted to find out the ability of Cantella asiatica inenhancing on the level interluekine (IL)-6 following challenge with Salmonella typhi. It istherefore expected that herbal drug such as Cantella asiatica can be used as an alternativemedicine to prevent and cure salmonellosis in both animals and human.Experimental laboratory studies were conducted using Completely FactorialRandomized Design. Mice were divided into 4 groups and they were treated respectively withdestilated water (negative control), 125, 250, and 500 mg/kg bw of Centella asiatica extract.The treatment was conducted daily for 2 weeks and the mice were then inoculated with 105cells of S. typhi. The level of IL-6 response were examined 24 hours, 2 weeks, and 4 weeksafter inoculation with S. typhi.The result showed that treatment of mice with Centella asiatica extract significantly(p<0,05) enhaced IL-6 level of Balb/c mice following inoculation with S. typhi. The highestIL-6 level were observed in mice treated Centella asiatica extract at the dose of 500 mg/kgBW (385,9257±125,4314 pg/ml serum). And the highest IL-6 level were observed at 2 weeksfollowing inoculation with S. typhi (533.4262 ± 81.7184 pg / ml).A further study is recomended to examine the celluler immune response and moredetailed study on the humoral immune response of animals or human before this herbal isused as alternatif medicine to prevent and cure typhoid fever. It is also important to study thebest preparation, the half life, and the side effect of Centella asiatica in human and animals.
Co-Authors Abdul Azis Nasution Abdul Azis Nasution Adinda Adinda Adryani Ris Agung, Mochamad Bale Aida Lousie Tenden Rompis Alda dasril lumban gaol Amalia, Ainun Rizki Arief Boediono BASYOFI DWIWANDANA Betharia Criselda Fanggidae Bibiana W Lay Calvin Iffandi Calvin Iffandi Debora Selfia Br Manurung Dewi, Ni Made Anindya Kumala Elpira Sukaratha, Elpira Febio Tomasini Marciano Meus Gde Angga Caka Primanditha, Gde Angga Caka Gunawan, I Wayan Nico Fajar Harry Yoga Nugraha HARTANINGSIH - Hartaningsih . Herbert . Herbert . I Gede Bim Shiddi Prama Putra I Gede Soma I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Ngurah Bagus Surya Dharma, I Gusti Ngurah Bagus I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Made Edi Suryawan I Made Edy Susanta I Made Kardena I Made Sukada I Made Yoga Windu Pradana i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Putu Gede Yudhi Arjentinia I Putu Sampurna I Wayan Batan I Wayan Suardana I. H. U Utama Imam Sobari imam sobari INNA RAKHMAWATI Ita Djuwita IW. Piraksa Ketut Adnyane Mudite Kusumaning Arumsari Wimbavitrati Lestari, Ayu Paramita Luh Gde Sri Surya Heryani Marcy Lapik, Siereh Eugene Meilendry Angelina Sigiro Minda Nealma Muh Imam Subiarsyah Natalia, Grace Kristin Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Made Devityasih Perayadhista Ni Nyoman Janipa Saptayanti Ni Nyoman Werdi Susari Ni Putu Dewi Setia Sari Ni Wayan Listyawati Palgunadi Ni Wayan Listyawati Palgunadi Nining Handayani Nining Handhayani NURUL FAIZAH Nurul Faiziah Nurul Faiziah P. Sampurna P. Suastika Praing, Umbu Yabu Anggung Putu Sampura Rasdi yanah Rasdiyanah . Rasdiyanah . Santi, Elysabeth Vanessa Tirta Sembiring, Ade Vindha Mebrina br Sri Kayati Widyastuti Sri Milfa Sri Milfa Supar - Umi Reston Wahono Esti Prasetyaningtyas Wahono Esti PrasetyoningtyaserB Wibisono, Hanif Wahyu Winardi, Rian Yudeska, Citra Yulia Khalifatun Nissa Yunita Lestyorini Yunita Lestyorini