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All Journal Jurnal Kesehatan Masyarakat Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education Jurnal Kesehatan Jaringan Laboratorium Medis Borneo Community Health Sevice Journal JHE (Journal of Health Education)
Anung Sugihantono
Pemerintah Daerah Provinsi Jawa Tengah
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health

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Journal : Jaringan Laboratorium Medis

 1 
Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Masa Simpan Test Sel A dan Test Sel B Hari Ke-0, Ke-2, Ke-4, Ke-6 dan Ke-8 Zatalini, Karinta Syifa; Kuncara, Rachmad Bayu; Sugihantono, Anung
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11733

Abstract

Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.
Kadar Kadmium (Cd) dalam Urine pada Perokok Pasif Nurhayana, Nurhayana; Sugihantono, Anung
Jaringan Laboratorium Medis Vol. 3 No. 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i2.8054

Abstract

Smoking is a familiar activity in daily life. The existence of filters on cigarettes, causes the danger of cigarette smoke to be felt more by passive smokers. One of the content of cigarette smoke is cadmium (Cd) which is one of the environmental pollutants that are harmful and accumulate in the tissues of living things. The purpose of this study is to measure and describe the levels of Cadmium (Cd) in the urine of passive smokers in RT 03 RW 06 Gandekan Hamlet, Harjosari, Bawen District, Semarang Regency. Using observational (non-experimental) research design with descriptive research criteria. The number of samples of 12 urine from the community of RT 03 RW 06 Gandekan Hamlet, Harjosari, Bawen Subdistrict, Semarang Regency. The results of the examination of Cadmium Levels (Cd) on the 13 samples of passive smoker urine in gandekan RT 01 RW 06 Harjosari Subdistrict, Bawen Subdistrict, Semarang District received the highest level of 0.074 mg/L and the lowest result -0.005 mg/L. Differences in cadmium levels in the passive smoker urine were caused by several factors such as age, environment, health status, occupation, and consumption of antioxidants and milk. Cadmium (Cd) levels in the urine of all passive smokers were in the normal category (<2mg/L).
Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Masa Simpan Test Sel A dan Test Sel B Hari Ke-0, Ke-2, Ke-4, Ke-6 dan Ke-8 Zatalini, Karinta Syifa; Kuncara, Rachmad Bayu; Sugihantono, Anung
Jaringan Laboratorium Medis Vol. 6 No. 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11733

Abstract

Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.
Co-Authors Ahmad Thohir Hidayat Anastasia Wulandari, Anastasia Azahra, Sresta Bagoes Widjanarko Birahmatika, Fitya Safira Djoko Priyatno, Djoko Duri, Iin Desmiany Faozan, Amir Fauzia, Khalida Handayani, Novia Helda Khusun Judhiastuty Februhartanty Kasiyati, Menik Kuncara, Rachmad Bayu Muchtaruddin Mansyur N. Nurjanah Nuniek Nizmah Fajriyah Nur Fadhilah Nurhayana, Nurhayana Nurlita, Hera Nurul Qomariyah Pramesthi, Indriya Laras Puspitaningtyas, Sheila Indah Roni Afriansya Sasangka, Parisade Galih Setyorini, Ria Novita Subekti, Ratih Syamsulhuda BM Teguh Budiharjo, Teguh Widodo Widodo Wiwit Sulistyasmi Yulian Wahyu Permadi Zatalini, Karinta Syifa