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POTENSI ANTIOKSIDAN DARI EKSTRAK ETANOL TONGKOL JAGUNG (Zea mays L.) Lumempouw, Liemey Iviane; Paendong, Jessy; Momuat, Lidya Irma; Suryanto, Edi
CHEMISTRY PROGRESS Vol 5, No 1 (2012)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.5.1.2012.654

Abstract

Lumempouw dkk., 2012. Potensi Antioksidan Dari Ekstrak Etanol Tongkol Jagung (Zea Mays L.). Tujuan penelitian ini adalah untuk menganalisis kandungan fitokimia dan menguji aktivitas antioksidan dariekstrak tongkol jagung. Tongkol jagung diekstrak dengan pelarut etanol 20, 40, 60 dan 80% dengan cara reflukselama 2 jam pada 78-90 oC. Setelah itu, ekstrak dianalisis kandungan fitokimia fenolik, flavonoid dan tanin.Pengujian aktivitas antioksidan menggunakan radikal bebas DPPH, sedangkan penentuan kapasitasantioksidan menggunakan metode FRAP dengan spektrofotometri UV/Vis. Hasil penelitian menunjukkan bahwatotal kandungan fenolik tertinggi terdapat pada ekstrak etanol tongkol jagung (E80), diikuti oleh E60, E40 andE20, sebaliknya E80 menunjukkan kandungan total flavonoid terendah dan tanin terkondensasi yang tidakterdeteksi. Ekstrak E80 menunjukkan aktivitas penangkal radikal bebas tertinggi dibandingkan E60, E40 andE20. Nilai IC50 ekstrak E80 menunjukkan paling rendah pada ekstrak etanol (E60, E40 dan E20) tongkol jagung.Ini berarti bahwa E80 memiliki aktivitas penangkal radikal bebas paling tinggi dalam ekstrak tersebut. Hasilpengukuran kapasitas antioksidan (FRAP) dari ekstrak E80 juga menunjukkan paling tinggi daripada E60, E40and E20. Hasil penelitian ini menyimpulkan bahwa tongkol jagung memiliki kandungan fitokimia fenolik danflavonoid yang dapat berpotensi sebagai antioksidan.Kata kunci : tongkol jagung, antioksidan, radikal bebas, fitokimia fenolikLumempouw et al., 2012. The Potency Of Antioxidant From Etanol Extract Of Corncob. Objectives of this research were to analyze phytochemical content and to examine antioxidant activity ofcorncob. Phytochemical antioxidant of corncob was extracted using ethanol 20, 40, 60 and 80% by reflux for 2hours at 78-90 oC. Afterwards, extract was analyzed of phytochemical phenolic, flavonoid and condensed tannincontent. Examination of antioxidant activity of use DPPH free radical and antioxidant capacities (FRAP) wereused spectrophotometry method. The highest total phenolic content was in ethanol extract (E80) of corncob,followed by E60, E40 and E20, conversely E80 show the lowest total flavonoid content and condensed tannintotal is not detected. Extract of E80 show the highest free radical scavenging activity compare to E60, E40 andE20. IC50 values of E80 extract showed the lowest was in ethanol extract (E60, E40 dan E20) of corncob. Itsmean that E80 has the highest free radical activity was in that extract. Antioxidant capacities (FRAP) on E80 ofcorncob showed also the highest than E60, E40 and E20. This result concluded that corncob possessphytochemical phenolic and flavonoid content potency able to as antioxidant.Keywords : corncob, antioxidant, free radical, phenolic phytochemical
AKTIVITAS ANTIFOTOOKSIDASI NANOPARTIKEL PERAK YANG DISINTESIS MENGGUNAKAN KULIT PISANG KEPOK (Musa Paradisiaca L.) Colling, Erningsi; Suryanto, Edi; Wuntu, Audy
CHEMISTRY PROGRESS Vol 11, No 2 (2018)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.11.2.2018.27943

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui Aktivitas antifotooksidasi nanopartikel perak yang disintesi menggunakan kulit pisang kepok. Larutan perak nitrat (AgNO3) 1mM direduksi menggunakan ekstrak kulit pisang kepok yang didiamka selama 15 menit pada ruang gelap. Karakterisasi menggunakan spektrofotometer UV-Vis dan Scanning Electron Microscope (SEM). Analisis terhadap stpektra UV-Vis menunjukan bahwa nanopartikel relatif stabil pada panjang gelombang 414.50-447.00 nm. Hasil dari karakterisasi SEM menunjukan nanopartikel perak yang disintesis menggunakan ekstrak kulit pisang kepok dengan perak nitrat (AgNO3) 1mM memiliki ukuran terkecil 46 nm dan yang terbesar mencapai 65 nm. Hasil penelitian ini menunjukan bahwa nanopartikel perak yang disintesis menggunakan ekstrak kulit pisang kepok memiliki kandungan fenolik dan aktivitas antiofotooksidasi lebih rendah dibandingkan dengan ekstrak kulit pisang kepok tanpa nanopartikel perak. ABSTRACTThis study aims to determine the antiphotooxidation activity of silver nanoparticles synthesized by kepok banana peel. Silver nitrate (AgNO3) 1mM solution was reduced using kepok banana peel extract which was stored for 15 minutes in a dark room. Characterization used UV-Vis spectrophotometer and Scanning Electron Microscope (SEM). Analysis of UV-Vis spectrum showed that nanoparticles were relatively stable at wavelength 414.50-447.00 nm. The results of SEM characterization showed that silver nanoparticles synthesized by 1mM kepok banana peel extract with silver nitrate (AgNO3) had the smallest size of 46 nm and the largest reached 65 nm. The results of this study showed that silver nanoparticles synthesized by kepok banana peel extracts had a lower phenolic content and antiophotooxidation activity compared to kepok banana peel extract without silver nanoparticles.
AKTIVITAS ANTIFOTOOKSIDASI DAN FOTOPROTEKSI FRAKSI PELARUT DARI DAUN GEDI (Abelmuschus manihot L) Abigael, Reliana; Suryanto, Edi; Katja, Dewa G.
CHEMISTRY PROGRESS Vol 12, No 1 (2019)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.12.1.2019.27914

Abstract

ABSTRAK Penelitian ini bertujuan untuk menentukan aktivitas antifotoksidasi mengukur nilai SPF dari ekstrak metanol daun Gedi (Abelmoschus manihot L). Daun gedi diekstraksi dengan cara sokletasi.  Dalam penelitian ini sampel diekstraksi dengan pelarut metanol, difraksinasi dengan pelarut petroleum eter, etil asetat, butanol dan akuades. Dari hasil fraksinasi tersebut kemudian diuji aktivitas antifotooksidasi dan fotoprotektif. Kandungan fenolik menggunakan asam galat sebagai larutan standart. Uji Aktivitas antifotooksidasi ditentukan dengan menghitung nilai diena terkojugasi yang merupakan pembentukan awal oksidasi pada lipid. Semakin rendah nila diena terkonjugasi, maka semakin baik sebagai antifotooksidasi. Untuk penentuan fotoprotektif, dilakukan dengan mneggunakan spektrofotometer UV-Vis pada panjang gelombang 290-320 nm dengan konsentrasi 100 μg/mL. Hasil penelitian ini menunjukkan bahwa fraksi etil asetat memiliki kandungan fenolik, aktivitas antioksidan, antifotooksidasi, dan fotoprotektif yang paling tinggi. Hasil yang diperoleh menunjukkan total fenol paling tinggi pada fraksi eti asetat yaitu 110.204 µg/mL, sedangkan untuk aktivitas antioksidan 57.72%. Hasil pada aktivitas antifotooksidasi menunjukkan bahwa fraksi Etil asetat menunjukkan kemampuan yang paling baik dan untuk fotoprotektik memiliki nilai SPF yang tinggi adalah etil asetat yaitu 9.03.  ABSTRACT This study aimed to determine the antifotoxidation activity measuring the SPF value of the solvent fraction of the leaves of Gedi (Abelmoschus manihot L). Gedi leaves extracted by socletation. In this study the samples were extracted with methanol solvent, fractionated with petroleum ether, ethyl acetate, butanol and aquades. The results of the fractionation were then tested for antifotooxidation and photoprotective activity. Phenolic content uses gallic acid as a standard solution. The antifotoxidation activity test is determined by calculating the conjugated diene value which is the initial formation of oxidation in the lipid. The lower the conjugated diene tilapia, the better it is antifotoxidation. For photoprotective determination, a UV-Vis spectrophotometer was carried out at a wavelength of 290-320 nm with a concentration of 100 μg / mL. The results of this study indicate that the highest ethyl acetate fraction has the highest phenolic content, antioxidant activity, antifotoxidation and photoprotective. The results obtained showed the highest total phenol in the eti acetate fraction of 110,204 µg / mL, while for antioxidant activity 57.72%. The results of the anti photoxidation activity showed that the ethyl acetate fraction showed the best ability and for the photoprotectic the high SPF value was ethyl acetate namely 9.03.  
SKRINNING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN DAN TABIR SURYA PADA TANAMAN JAGUNG (Zea mays L.) Pangemanan, David Albert; Suryanto, Edi; Yamlean, Paulina V. Y.
PHARMACON Vol 9, No 2 (2020): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.9.2020.29271

Abstract

ABSTRACT Corn (Zea Mays L.) is widely developed in Indonesia. Corn kernels showed total phenolic and antioxidant activity. Antioxidants have the potential as photoprotectors, therefore, plants containing phenolic compounds can be used in the prevention of free radicals. For this reason, researcher is interested in further researching the phytochemical screening, antioxidant activity and sunscreen testing from extracts of stems, leaves, hair and leaf wrapping from corn (Zea mays L.). The samples used were corn plants (corn silk, corn leaves, corn stalks and leaf wrapping corn) originating from Kauditan area, North Minahasa. The results of the study showed that the leaf contained saponin and flavonoid compounds, the stem contained alkaloid and saponin compounds, the hair contained alkaloid and flavonoid compounds, and the leaf wrapping only contained alkaloid compounds. The highest to lowest total phenolic content values starting from the stem were 46.93 μg / mL, the leaf wrapping 37.76 μg / mL, the leaf 26.63 μg / mL and hair 14.49 μg / mL. The leaf has the highest antioxidant activity with a value of 72.81% followed by 62.87% stem then wrapping leaf 43.13%  and finally hair 29.14%. The SPF value of the stem was included in the ultra protection with a value of 16.117 followed by the leaves included in the maximum protection with an SPF value of 10.902. While the hair with SPF value of 0.6 and wrapping leaf with SPF value of 0.222 are included in the minimum protection. Keywords: Corn (Zea Mays L.), Sunscreen, Antioxidants, Phenol ABSTRAK Jagung (Zea Mays L.) banyak dikembangkan di Indonesia. Biji jagung menunjukkan kadar total fenolik dan aktivitas antioksidan. Antioksidan memiliki potensi sebagai fotoprotektor, oleh karena itu, tanaman yang mengandung senyawa fenolik dapat digunakan dalam pencegahan radikal bebas. Untuk itu peneliti tertarik untuk meneliti lebih lanjut tentang skrining fitokimia, uji aktivitas antioksidan dan tabir surya dari ekstrak batang, daun, rambut, dan daun pembungkus dari jagung (Zea mays L.). Sampel yang digunakan ialah tanaman jagung (rambut jagung, daun jagung, batang jagung dan daun pembungkus jagung) yang berasal dari daerah Kauditan, Minahasa Utara. Hasil dari penelitian menunjukkan daun mengandung senyawa saponin dan flavonoid, batang mengandung senyawa alkaloid dan saponin, rambut mengandung senyawa alkaloid dan flavonoid, dan daun pembungkus hanya mengandung senyawa alkaloid. Nilai kandungan total fenolik  yang paling tinggi sampai paling rendah dimulai dari batang sebesar 46.93 μg/mL, daun pembungkus 37.76 μg/mL, daun 26.63 μg/mL dan rambut 14.49 μg/mL. Daun memiliki aktivitas antioksidan yang paling tinggi dengan nilai 72.81% diikuti oleh batang 62.87% kemudian daun pembungkus 43.13% dan yang terakhir adalah rambut 29.14%. Nilai SPF batang termasuk dalam proteksi ultra dengan nilai 16,117 diikuti oleh daun yang termasuk dalam proteksi maksimal dengan nilai SPF 10,902. Sedangkan rambut dengan nilai SPF  0,6 dan daun pembungkus dengan nilai SPF 0,222 termasuk dalam proteksi minimal. Kata Kunci : Jagung (Zea Mays L.), Tabir Surya, Antioksidan, Fenol
UJI AKTIVITAS ANTIOKSIDAN DARI AMPAS HASIL PENGOLAHAN SAGU (Metroxylon sagu Rottb) Talapessy, Selvian; Suryanto, Edi; Yudistira, Adithya
PHARMACON Vol 2, No 3 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.2373

Abstract

ABSTRACTThe study aimed to determine the phenolic content and antioxidant activity of sago wasteprocessing results. Results of processing sago pulp esxtraction was conducted using 1 x 24hour maceration using solvents ethanol with a concentration of 20, 40, 60 and 80%. Phenoliccontent was determined by Folin Ciocalteu method and determination of antioxidant activityusing the DPPH free radical scavengers. The results showed that the extract with the solventconcentration of 40% has a higher total phenolic 83,26 μg/mL followed by solvent extractconcentration of 20, 60 and 80% respectively 70,01; 60,58; 22,04 μg/mL. extract with 60%solvent concentration has free radical scavengers activity the highest of 82,75% followed byconcentration of solvent extracts 80, 20 and 60% are respectively 80,45; 76,62; 62,83%.These results suggest that the processing of sago pulp extract contains phenolic antioxidantsand can be potentially as.Keywords : pulp extract sago precessing results, phenolic compend, antioxidants.
ANALISIS FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DARI GETAH KULIT BUAH PISANG GOROHO (Musa acuminate (L.)) Kurniawan, Juliana Christie; Suryanto, Edi; Yudistira, Adithya
PHARMACON Vol 2, No 3 (2013): pharmacon
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.2.2013.2330

Abstract

ABSTRACTThe objectives of this research was to analyze the phytochemical content and antioxidantactivity from goroho’s sap peel. Sample preparation was cut from the banana tier, then havebeen cut to the both base of goroho’s peel and the fresh sap collected into the petri dish. Theextraction method was maceration used 20, 40, 60 and 80% ethanol concentration.Phytochemical screening carried out by analysis of alkaloid, flavonoid, saponin, steroid,terpenoid and tannin. Determination of antioxidant activity performed by DPPH free radicalscavengers. The phytochemical analysis results was goroho’s sap peel contain flavonoid,saponin and tannin. Ethanol extract of goroho’s sap peel with 80% concentration 100 μg/mLhad the highest antioxidant activity. Results of this research concluded that phenolic extractsof goroho’s sap peel has the ability both as an antioxidant to DPPH free radicals.Keywords: Banana sap peel goroho, phytochemical, antioxidant activity
AKTIVITAS ANTIOKSIDAN DARI EKSTRAK TONGKOL JAGUNG (Zea mays L.) Saleh, Lisa Purnawati; Suryanto, Edi; Yudistira, Adithya
PHARMACON Vol 1, No 2 (2012)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.1.2012.465

Abstract

The objective of this research was to determine the potential antioxidant phytochemicalextracts corn cob (Zea mays L.). Corn cobs extracted using maceration method for 1x24 hours.Determination of phytochemical content is done by calculating the total phenolic. Testingantioxidant activity using free-radical scavengers DPPH (1,1-diphenyl-2-pikrilhidrazil). Results ofthis research showed that the extract of corn cobs have total phenolic. Extract 60% corn cobs haveprophylactic activity of free radicals, followed by extract 40, 80 and 20%. Conclusion of thisresearch is corn cob extracts have potential as antioxidants phytochemicals.Keywords : corn cob, antioxidant, phenolic, DPPH
KOMPOSISI KIMIA DAN AKTIVITAS ANTIOKSIDAN DARI PARING KELAPA (COCOS NUCIFERA) Karepu, Meifry Gavrila; Suryanto, Edi; Momuat, Lidya I.
CHEMISTRY PROGRESS Vol 13, No 1 (2020)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.13.1.2020.29604

Abstract

Penelitian ini bertujuan untuk mengkarakterisasi secara kimia dan menentukan aktivitas antioksidan dari paring kelapa yang diekstraksi dengan masing-masing pelarut petroleum eter (PPE) dan etanol (PET). Penelitian ini terdiri dari tiga tahap yaitu mikronisasi, karakterisasi, dan ekstraksi. Parameter yang digunakan adalah komposisi proksimat, serat kasar, serat pangan, kandungan hemiselulosa, selulosa, lignin, aktivitas antioksidan, dan kapasitas penangkal nitrit. Hasil karakterisasi secara kimia menunjukkan tepung paring kelapa yang diekstraksi dengan pelarut petroleum eter mengandung komposisi kimia seperti air (6,99%), abu (0,85%), lemak (3,61%), protein (10,39%), serat pangan tak larut (78,27%), serat pangan terlarut (2,66%), serat pangan total (80,93%), hemiselulosa (25,38%), selulosa (39,15%) dan lignin (24,55%). PET mengandung air (7,14%), abu (1,03%), lemak (6,05%), protein (9,85%), serat pangan tak larut (73,795%), serat pangan terlarut (2,53%), serat pangan total (76,32%), hemiselulosa (20,97%), selulosa (35,14%) dan lignin (32,07%). ­ Hasil pengujian aktivitas antioksidan menunjukkan bahwa PET memiliki ekstrak fenolik bebas (92,16%) tertinggi diikuti oleh ekstrak fenolik terikat PPE (87,75%), ekstrak fenolik bebas PPE (85,07%) dan ekstrak fenolik terikat PET (81,90%). ABSTRACT The objectives of this research were to characterize the physicochemical properties and to determine the antioxidant activity of coconut testa dietary fiber. The research consisted of 3 stages: micronization, characterization, and extraction. The evaluate parameters were proximate composition, crude fiber, dietary fiber, hemicellulose, cellulose, lignin, antioxidant activity, and nitrate-scavenging capacity. Chemical characterization results showed that coconut testa powder has chemical compositions such as water (6,99%), ash (0,85%), fat (3,61%), protein (10,39%), carbohydrate (48,75%), insoluble dietary fiber (78,27%), soluble dietary fiber (2,66%), total dietary fiber (80,93%), hemicellulose (25,38%), cellulose (39,15%) and lignin (24,55%). PET contains water (7.14%), ash (1.03%), fat (6.05%), protein (9.85%), insoluble food fiber (73.795%), dissolved food fiber (2.53 %), total food fiber (76.32%), hemicellulose (20.97%), cellulose (35.14%) and lignin (32.07%). Antioxidant activity assay showed that free phenolic extract of PET had highest (92.16%) followed by bound phenolic extract of PPE (87.75%), free phenolic extract of PPE (85.07%) and bound phenolic extract of PET (81.90 %).
Karakterisasi dan Aktivitas Antioksidan Tepung Sagu Baruk (Arenga microcarpha) Tarigan, Elisa Putri; Momuat, Lidya Irma; Suryanto, Edi
Jurnal MIPA Vol 4, No 2 (2015)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.4.2.2015.9036

Abstract

Tujuan penelitian ini adalah untuk mempelajari karakteristik kimia tepung sagu baruk yang mengandung senyawa fitokimia dan mempelajari aktivitas antioksidan dari ekstrak tepung sagu baruk. Tepung sagu baruk diekstrak dengan pelarut etanol 50% dengan cara maserasi selama 24 jam. Setelah itu, tepung sagu diuji  proksimat, ekstrak dianalisis kandungan fitokimia fenolik, flavonoid dan tanin terkondensasi. Pengujian aktivitas antioksidan menggunakan radikal bebas DPPH dan daya reduksi dengan spektrofotometer UV/Vis. Hasil penelitian menunjukkan pada pengujian proksimat bahwa tepung tepung sagu baruk memenuhi syarat Standar Nasional Indonesia, tetapi berbeda dengan kadar serat kasar. Total kandungan fenolik dan flavonoid  menunjukkan bahwa tepung sagu akuades (SA) lebih tinggi daripada tepung sagu komersial (TSBK). Hasil radikal bebas sagu tepung sagu SA lebih tinggi dari TSBK sama halnya dengan kemampuan daya reduksi SA jauh lebih tinggi dari TSBK. Hasil penelitian ini menyimpulkan bahwa tepung sagu baruk memiliki kandungan fitokimia fenolik dan flavonoid yang dapat berpotensi sebagai antioksidan.Objectives of this research were to study the chemical characteristics of sago baruk flour containing phytochemicals and antioxidant activity of sago baruk flour extract. Sago baruk flour was extracted with 50% ethanol by maceration for 24 hours. Afterward, sago flour was tested for proximate and the extract was analyzed for phenolic, flavonoids, and condensed tanin. DPPH free radical was used to examine antioxidant activity and spectrophotometry method was used to determine the reduction ability. The result for proximate test showed that sago flour extract meets Indonesian Nasional Standard requirements. Total phenolic and flavonoid contents in the flour extracted with aquadest (SA) were higher than that in commercial flour (TSBK). Free radical scavenging activity of sago flour SA was higher than that of TSBK, as well as the reduction ability of SA that was much higher than of TSBK. It was concluded that sago baruk flour possess phytochemical content of phenolic and flavonoid which were potential as antioxidant.
Perbandingan Aktivitas Antioksidan Dari Tongkol Jagung (Zea mays L.) Segar dan Kering Dengan Metode Refluks Saryana, Ryan Vanly; Suryanto, Edi; Sangi, Meiske S.
Jurnal MIPA Vol 3, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.3.2.2014.5858

Abstract

Tujuan pada penelitian ini adalah menentukan potensi antioksidan ekstrak air dari tongkol jagung. Tongkol jagung yang sudah dikering anginkan digiling sedangkan yang segar hanya dicincincang hingga halus kemudian diekstraksi menggunakan metode refluks selama 0, 1, 2, 3 dan 4 jam menggunakan pelarut akuades. Analisis kandungan total fenolik, flavonoid dan tanin terkondensasi menggunakan spektrofotometer UV-Vis. Untuk penentuan penangkal radikal bebas menggunakan metode 1.1-diphenyl-2.-picrylhydrazyl (DPPH). Hasil dari penelitian ini menunjukkan bahwa tongkol jagung kering memiliki kandungan total senyawa fenolik, flavonoid dan DPPH  terbaik dengan pemanasan selama 4 jam. Akan tetapi total tanin terkondensasi terbaik terdapat pada tongkol jagung segar dengan pemanasan selama 4 jam.The purpose of this study was to determine the antioxidant potential of water extract of corn cobs. Corn cobs wind dried milled while fresh only chopped until smooth then extracted using the reflux method for 0, 1, 2, 3 and 4 hours using distilled water solvent. Analysis of total phenolic content, flavonoids and condensed tannins using UV-Vis spectrophotometer. For the determination of free-radical scavengers using the method of 1.1-diphenyl-2-picrylhydrazyl (DPPH). The results of this study indicate that the dried corn cobs have the best content of total phenolic compounds, flavonoids and DPPH by heating for 4 hours. But, the total condensed tannins are best fresh on corn cob with heating for 4 hours.
Co-Authors Abigael, Reliana Adisti A. Rumayar, Adisti A. Adithya Yudistira Adrian, Gerild Aliwu, Indriwati Alvin Febryanto Ginting, Alvin Febryanto Amelia Walangitan Aneke Korua, Aneke Assa, Jan Rudolf Audy Denny Wuntu Bolilera, Theressa Chairil Anwar Chaleb Paul Maanari Christine Mamuaja Colling, Erningsi Dareda, Christina Tang Defny S. Wewengkang, Defny S. Dewa Gede Katja Dika, Oki Oktaviani Djasibani, Hemy R. Dokri Gumolung Edam, Mariati Edam, Mariati Elisa Putri Tarigan, Elisa Putri Ering, Aprily A. P. Etni Togolo Fahcrudin, M Fajar Lestari Fetty Indriaty Fiky Andy Prastyawan FITRIA NINGRUM, FITRIA Frenly Wehantouw Gregoria S. S. Djarkasi Harry S. J. Koleangan Hasan Datu, Hasan Henry Aritonang Hindang S. Kaempe Indriawan, Rebecca Teisha Inneke F. M Rumengan Jeanette Manarisip Jemmy Abidjulu Jessy Paendong Johnly A Rorong Johnly A. Rorong Judith Mandei Juliana Christie Kurniawan Julius Pontoh Kabalmay, Julista Alfa Kaemba, Almawaty Kaemba, Almawaty Karepu, Meifry Gavrila Karisoh, Marsel Refanli Landeng, Patrisia Jaklin Landjang, Enrico Yoel Leo Arifsandi Budiarso, Leo Arifsandi Lexie Mamahit Lidya I. Momuat Lidya Irma Momuat Liemey Iviane Lumempouw Liemey Lumempouw Lies Mira Yusiati Lisa Purnawati Saleh Luayli, Lina Lucia Cecilia Mandey Makanaung, Eresia Mamuaja, Christin Mandalurang, Fitriani Manongko, Patricia Syaron Max R. J. Runtuwene Meiske Lumingkewas Meiske S. Sangi Mercy Taroreh Momuat, Lidya Mongan, Jemsi Muhammad Ilham Nancy Kiay, Nancy Nova . Olha Rantung, Olha Paijo, Auliya Rizky Harjono Palente, Ivon Pangemanan, David Albert Parinding, Yosep Rudol Pasanda, Immanuel M. Paulina yamlean Raganata, Tirza C. Riny Modaso Rizky H. Mawardi Robert Molenaar Roisu Eny Mudawaroch Rompas, Veckie Frankie Rondonuwu, Samuel D. J. Rorong, Johnly Alfrets Runtuwene, Max R. J Runtuwene, Max Revolta Jhon Ryan Vanly Saryana Sakalaty, Evanda Sakti, Dwi Selvian Talapessy Sembiring, Elia Sersermudy, Chesya Hana Setiyono Setiyono Shirley E. S. Kawengian Sri Febriani Hatam Sri Sudewi, Sri Suci Paramitasari Syahlani Susmantoyo, Adinda Putri Maharani Suyanto Suyanto SYARIFUDDIN KADIR Tapa, Fitriyanti La Taufik Hidayat Trina Tallei Waworuntu, Mutiara G. Wiranto, Rezky Ramadhani Putri Yenny Tambunan Yuny Erwanto