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Screening and profiling of mercury-resistant Azotobacter isolated from gold mine tailing in Pongkor, West Java Suryatmana, Pujawati; Handayani, Sri; Bang, Sunbaek; Hindersah, Reginawanti
Journal of Degraded and Mining Lands Management Vol. 11 No. 2 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.112.5287

Abstract

Mercury (Hg) is a hazardous pollutant produced during the amalgamation of gold extraction. The environmental problems related to improper Hg waste management have become progressively concerning. Hg contamination in environments can be removed by using bioremediation technology. Utilizing Hg-resistant (HgR) microorganisms in Hg bioremediation is a crucial strategy. Azotobacter is one of the potential microbes for Hg bioremediation bioagent due to exopolysaccharides synthesis that binds the heavy metal. The study's main objective was to select and profile a novel Hg-resistant Azotobacter isolated from heavily Hg-contaminated soil and tailing of artisanal and small-scale gold in Pongkor area, West Java, Indonesia. The completely randomized design was used for profiling Azotobacter-HgR and included Hg values of 0, 1, 10, 100, 200, and 400 mg/L. Further, Azotobacter isolate bioassay steps included soil contaminated with Hg, soil contaminated with Hg + Azotobacter sp. S6.a, soil contaminated with Hg + consortium. The profiling results revealed that four Hg-resistant isolates were Azotobacter sp. S5, Azotobacter sp. S6, Azotobacter sp. S6.a, and Azotobacter sp. S9. More importantly, Azotobacter sp. S5 followed by Azotobacter sp. S6.a was found to be the most resistant to Hg exposure at a concentration of 400 mg/L. The Azotobacter sp. S9. produced the lowest EPS, but had the highest activity of nitrogenase and organic acid production. Meanwhile, Azotobacter sp. S6.a. produced the highest EPS. Isolate S5 showed the highest potential as a resistant PGPR-Hg isolate for enhancing the growth of sorghum in Hg-contaminated soil. Sorghum plants accumulate Hg from the soil in the roots but not in the shoots. Present findings suggest that these two isolates have the potential to be used as biological agents to rehabilitate Hg-contaminated soil in Pongkor area.
The effect of cow manure amendment and the ratio of gold mine tailings on sweet potato gold phytomining Noviardi, Rhazista; Joy, Benny; Sudirja, Rija; Setiawati, Mieke Rochimi; Djuwansah, Muhamad Rachman; Karuniawan, Agung; Sofyan, Emma Trinurani; Suryatmana, Pujawati
Journal of Degraded and Mining Lands Management Vol. 12 No. 1 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.121.6665

Abstract

Artisanal and small-scale gold mining has commonly used the mercury amalgamation method for gold ore extraction. The processing waste or tailings often contain gold and other metals. This study examined the effects of cow manure amendment and the ratio of gold mine tailings on sweet potato     (Ipomoea batatas L.) gold phytomining. The pot experiment was conducted in the Faculty of Agriculture greenhouse at Padjadjaran University in Indonesia. The sweet potato MZ119 clone (an orange-fleshed sweet potato) was planted on a polybag (40 x 40 cm) consisting of 10 kg of planting media, which was a mixture of gold mine tailing and soil. The design used was a completely randomized design factorial with three replications. The first factor was ratios of gold mine tailing consisting of three levels, namely 30%, 50%, 70%, and 100% (w/w). The second factor was doses of organic manure, namely without cow manure, 250 g pot-1, 500 g pot-1, and 750 g pot-1. The results demonstrated that the application of cow dung and the gold mine tailings ratio (w/w) had a significant (p = 0.05) effect on plants' dry weight biomass, gold accumulation, and gold phytomining by sweet potato. The highest gold mass yield of 0.066 mg gold plant-1 was achieved by a 50% (w/w) gold mine tailing ratio and 750 g pot-1 cow manure treatment.
Response of ornamental plant Aster to inoculation of various inoculant of soil microbial consortia Hindersah, Reginawanti; Fitriatin, Betty Natalie; Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Aditya, Fasa; Nugraha, Gita Bina
Jurnal Agroekoteknologi Vol 16, No 1 (2024)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v16i1.24604

Abstract

The Aster (Callistephus chinensis L) is an important commodity and is cultivated by using NPK fertilizer. The other nutrient source in environmental-friendly agriculture is biofertilizer contains mixed strains of beneficial microbes. The purpose of a field experiment was to observe the influence of various biofertilizer composed of mixed strain of Nitrogen-fixer bacteria and Phosphate-solubilizer microbes on Aster plant growth and yield of flower. The research design was a randomized block design consisted of four biofertilizer types and one control. The results verified that biofertilizer increased plant height at 9 weeks after application but have not affected the initial generative stadia. The consortia of Pseudomonas diminuta, P. Cepaceae, Penicillium sp., and Aspergillus sp. showed the best effect on the plant health, flower number per plot, and flower diameter. The experiment confirmed that all biofertilizers could be used as the source of biostimulants for Aster. 
Effect of biofertilizer with reduced dose of nutrient solution on yield of Pak Choy on Nutrient Film Technique System Hindersah, Reginawanti; Maulana, Nurzen; Damayani, Maya; Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Fitriatin, Betty Natalie; Setiawan, Ade
Soilrens Vol 21, No 2 (2023)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v21i2.53445

Abstract

The Nutrient Film Technique (NFT) in hydroponic crop production systems generally uses chemical nutrient solutions to provide plant nutrients, even though biofertilizer inoculation can enrich liquid nutrients. The purpose of this experiment was to observe the effect of liquid biofertilizers applied with chemical nutrient solutions on changes in the acidity and electrical conductivity of nutrient solutions, as well as the growth and yields of pak choy (Brassica rapa L.) grown in Nutrient Film Technique. This experiment was set up in a completely randomized design with three nutrient solution treatments and eight replications. The treatments included the application of 100% chemical nutrient solution (control), and 75% and 50% concentrations of chemical nutrient solution enriched with a consortium biofertilizer. The results showed that at the end of the experiment, the acidity of all nutrient solutions increased, but their electrical conductivity (EC) was reduced. The fresh weight of pak choy shoots in medium B (75% chemical nutrition with biofertilizers) was 34% lower than the control (A), although statistically it was not significant. However, 50% chemical nutrition with biofertilizers produced the same shoot weight as the recommended dose of chemical nutrition. Moreover, 75% chemical nutrient with biofertilizers increased root weight. This current experiment verified that a reduced concentration of nutrient solution enriched with biofertilizer can be used to maintain the yield of pak choy grown in NFT.
Pengaruh Pupuk N Bio-organomineral terhadap pH, Nitrat, C-organik, Kandungan Pb Tanah dan Serapan Pb Tanaman Padi (Oryza sativa L.) pada Sawah Tercemar Limbah Tekstil Sudirja, Rija; Masruri, Muhammad Kholil; Suryatmana, Pujawati; Rosniawaty, Santi; Kamaluddin, Nadia Nuraniya; Sandrawati, Apong
Soilrens Vol 21, No 2 (2023)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v21i2.53453

Abstract

Lead pollution in rice fields in Rancaekek is attributed to the inadequate processing of waste from the textile industry. This phenomenon engenders a decline in soil fertility, thereby posing a threat to both food quality and agricultural sustainability. Employing N Bio-Organomineral fertilizer stands as one of the measures aimed at ameliorating the quality of rice fields afflicted by heavy metal contamination, particularly lead (Pb). This study seeks to elucidate the impact of N Bio-Organomineral fertilizer on various soil parameters, namely pH, NO3 - , soil organic carbon (C-organic), soil Pb solubility, and plant Pb uptake. The research was conducted in Linggar Village, Rancaekek, spanning from December 2019 to April 2020. Utilizing a Randomized Block Design as the experimental setup consisted of eight treatments with four replications, including: a control (0), urea at 250 kg/ha, and varying doses of the fertilizer at 250, 500, 750, 1000, 1250, and 1500 kg/ha. N Bio-Organomineral fertilizer represents a modified form of nitrogen fertilizer comprising urea, zeolite, activated charcoal, and compost enriched with Bacillus subtilis, formulated in a ratio of 60:20:10:10, respectively. The findings indicate that the application of N Bio-Organomineral fertilizer leads to an augmentation in soil NO3 - and C-organic content, while concurrently mitigating the uptake of Pb by plants. Among the treatments, the application of 500 kg/ha of N Bio-Organomineral fertilizer emerges as the most efficacious in enhancing soil NO3 - and C-organic content, as well as diminishing Pb accumulation in plants
Pengaruh Pupuk Hayati dan Azolla (Azolla pinnata) terhadap Beberapa Sifat Kimia Tanah, Pertumbuhan, dan Hasil Padi Sawah (Oryza sativa L.) Setiawati, Mieke Rochimi; Ilyas, Ichsan; Suryatmana, Pujawati; Setiawan, Ade
Soilrens Vol 21, No 2 (2023)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v21i2.53446

Abstract

Application of Biofertilizer and Azolla (Azolla pinnata) to Improve Several Soil Chemical Properties, Growth and Yield of Rice Plants (Oryza sativa L.). Efforts to increase agricultural production through intensification programs cannot be separated from using artificial chemical fertilizers. However, the continuous use of artificial chemical fertilizers must be balanced by providing organic matter to avoid a deficiency of land-available nutrients, organic matter, and beneficial microorganisms. Using biofertilizers and Azolla can help provide nutrients and improve the biological properties of the soil. This research aimed to determine the effect of the interaction of biofertilizer and Azolla in increasing soil N and lowland rice yields. This research was conducted in a greenhouse, experimental garden, Ciparanje, Jatinangor, Faculty of Agriculture, Universitas Padjadjaran. The experimental design used was a factorial randomized block design with two factors. The first factor, the dose of Azolla pinnata (A), consisted of 4 levels: without Azolla, Azolla 10 t ha-1, Azolla 20 t ha-1, and Azolla 30 t ha-1. In comparison, the second was the dose of solid biofertilizer (H), which consisted of 3 levels, without biofertilizer, 12.5 kg ha-1 biofertilizer, and 25 kg ha-1 biofertilizer, repeated three times. The results of the study showed that there was no interaction between biofertilizer and A. pinata on total soil N, plant N concentration, soil C/N ratio, and lowland rice (Oryza sativa L.) yields; however, the application of Azolla 30 t ha-1 increase the number of productive tillers. The application of biofertilizer and Azolla has yet to increase rice plants' Dry Harvested Grain (DHG). However, the DHG tends to increase by 9.58% and 9.95%, respectively, compared to the control
Perubahan Komposisi Mikrob dalam Proses Fermentasi Kopi Honey dan Natural Kamaluddin, Nadia Nuraniya; Solihin, Eso; Suryatmana, Pujawati; Januar, Dodi Ganjar; Rainaldi, Rainaldi; Setiawan, Ade
Soilrens Vol 21, No 2 (2023)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v21i2.53447

Abstract

This study investigates the microbial populations during different coffee fermentation processes and their impact on coffee quality. Bacillus, Pseudomonas, and endophytic populations in coffee beans undergoing natural saccharic and natural lactic fermentation remained unchanged compared to fresh coffee beans. However, a notable increase in Bacillus population occurred in honey saccharic fermentation, possibly attributed to the high sugar content in the mucilage, supporting bacterial growth. Additionally, the introduction of Lactobacillus and Saccharomyces during fermentation enhanced sugar consumption and influenced the final coffee quality, particularly aroma profile and nutritional composition. Varietal differences were observed, with Bacillus population decreasing slightly post-fermentation, especially in honey saccharic and natural lactic processes. This decline may be attributed to the dominance of Saccharomyces and Lactobacillus, antagonistic to Bacillus, and the fermentation conditions leading to decreased pH, unfavorable for Bacillus. These findings highlight the intricate microbial interactions and their implications for coffee fermentation and quality.
Pengaruh pupuk N Bio-organomineral terhadap pH, NO3-, C-organik, Na-dd, kandungan Pb tanah dan serapan Pb padi (Oryza sativa L.) pada sawah tercemar limbah tekstil Sudirja, Rija; Masruri, Muhammad Kholil; Suryatmana, Pujawati; Rosniawaty, Santi; Kamaluddin, Nadia Nuraniya; Sandrawati, Apong
Soilrens Vol 22, No 1 (2024)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v22i1.57231

Abstract

Lead (Pb) heavy metal pollution on rice fields in Rancaekek is caused by the disposal of textile industry waste which is not perfectly processed. The impact is a decrease in soil fertility which can threaten the food quality and agricultural sustainability. The application of N Bio-Organomineral fertilizer is one of the efforts to improve the quality of rice fields polluted with heavy metals Pb and high Na content. This research aims to find out the effect of N Bio-Organomineral fertilizer on pH, NO3-, Na, C-organic soil, solubility of soil Pb and uptake of plants Pb. The experimental design used a Randomized Block Design (RBD) with eight treatments and four replications consisting of: 0 (control); urea 250 kg/ha; and the variation in the dose of fertilizer is 250; 500; 750; 1000; 1250; 1500 kg/ha. N Bio-Organomineral fertilizer is a modification of N fertilizer based on urea, zeolite, activated charcoal and compost enriched with Bacillus subtilis with formulation 60:20:10:10. The results showed that the application of N Bio-Organomineral fertilizer could increase the NO3- and C-organic content of the soil and reduce the Na-dd and Pb uptake of plants. Treatment of 500 kg/ha N Bio-Organomineral fertilizer was the best dose in increasing the NO3- and Corganic content of the soil and reducing Na-dd and Pb uptake of plants
Potensi Jenis Bahan Pembawa (Carrier) Pemfiksasi N untuk Meningkatkan Pertumbuhan dan hasil Kedelai (Glycine max L.) pada Inceptisol Jatinangor Suryatmana, Pujawati; Wati, Dyah Aditya; Kamluddin, Nadia Nuraniya; Setiawati, Mieke Rochimi
Soilrens Vol 22, No 1 (2024)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v22i1.57232

Abstract

Sustainable agriculture is the proper solution for improving the fertility of Inceptisol, which is potentially suitable for soybean cultivation. Balanced fertilization, using both inorganic fertilizers and nitrogen-fixing biofertilizers, is one appropriate solution. To enhance the effectiveness of nitrogenfixing bacteria, a suitable carrier material is needed. This study aims to assess the potential of different carrier materials for Azotobacter and Bacillus on the growth and yield of soybeans on Inceptisol in Jatinangor. The research employs a Randomized Block Design with two factors: NPK dosage (0%, 50%, and 100%) and Azotobacter sp. and Bacillus sp. in various carriers (compost, bran, and Azolla powder) with 3 replicates. The results indicate that the interaction of Azotobacter sp. and Bacillus sp. in carriers had no significant effect on the height and chlorophyll content of soybean plants. The 100% NPK dosage yielded the highest number of pods compared to the 50% and 0% dosages. The treatment of Azotobacter and Bacillus in carriers did not significantly affect the number of filled pods, plant height, or chlorophyll content.
The Potential of Azolla pinnata Powder and Compost as a Carrier-base for Improving N-Fixing and P-Solubilizing Bacteria Performance to Increase Soybean Productivity Suryatmana, Pujawati; Halimah, Ummu ‘Azizah; Kmaluddin, Nadia Nuraniya; Herdiyantoro, Diyan; Setiawati, Mieke Rochimi
Agrikultura Vol 35, No 2 (2024): Agustus, 2024
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v35i2.55096

Abstract

Efektivitas inokulan Azotobacter sp. dan Bacillus sp. sering kali berkurang ketika diaplikasikan di tanah asam seperti Inceptisol, sehingga diperlukan upaya untuk meningkatkan kinerjanya dalam kondisi ini. Salah satu strategi adalah memilih bahan pembawa inokulan yang tepat seperti tepung Azolla pinnata dan kompos. Tujuan penelitian ini adalah untuk mengevaluasi kepadatan populasi inokulan, pembentukan bintil akar, berat kering akar, dan jumlah daun trifoliat pada tanaman kedelai yang diberi perlakuan tepung A. pinnata dan kompos sebagai carrier inokulan Azotobacter sp. dan Bacillus sp. Penelitian ini dilakukan pada Agustus 2022 hingga Januari 2023 di Laboratorium Biologi Tanah dan Kebun Percobaan Ciparanje, Departemen Ilmu Tanah dan Sumber Daya Lahan, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancanagan Acak Kelompok Faktorial dengan dua faktor yaitu faktor pertama berupa pemberian pupuk NPK dengan dosis 100% (300 kg/ha), 50% (150 kg/ha) dan 0% (0 kg/ha) dan faktor kedua adalah perlakuan inokulan kultur cair, inokulan dalam kompos, inokulan dalam tepung Azolla, inokulan dalam campuran kompos dan Azolla yang masing-masing diulang sebanyak tiga kali. Hasil penelitian menunjukkan bahwa carrier tepung Azolla dan kompos meningkatkan populasi Azotobacter spp. dan Bacillus spp. di rizosfer. Inokulan dalam campuran pembawa kompos dan tepung Azolla menghasilkan berat kering akar dan jumlah bintil tertinggi secara signifikan. Inokulasi kultur cair Azotobacter sp. dan Bacillus sp. atau inokulan padat dengan bahan pembawa tepung Azolla menghasilkan polong kedelai yang setara dengan aplikasi NPK 150 kg/ha. Aplikasi inokulan dalam tepung Azolla, kompos, atau campuran keduanya menghasilkan bobot 100 biji yang tidak berbeda nyata, namun, bobot 100 biji setara dengan perlakuan yang menggunakan dosis NPK 300 kg/ha di Inceptisol
Co-Authors . WISJNUPRAPTO Adawiah, Aliya Zahrah Ade Setiawan Aditya, Fasa Aditya, Fasa Aditya, Fasa Agung Karuniawan Agung Karuniawan Agung Muhammad Yusuf Aliya Zahrah Adawiah Alyani Shabrina Amalia Chusnul Anas Ramdhani Andi Hana Mufidah Elmirasari ANNE NURAINI Anne Nurbaity ANNE NURBAITY Anne Nurbaity Anni Yuniarti Annisa Rosalina Sylvia Apong Sandrawati Asmiran, Priyanka Aten Komarya Bagus Adityo Bang, Sunbaek Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Budiman, Muhammad Nafariz Chotimah, Andina Christine Angel Damayani, Maya Dedeh H. Arief Dedi Ruswandi Denny Sobardini Sobarna Denny Sobardini Sobarna Dewi, Yeni Wispa Dewikusuma Ikhsani Adhiningtyas Diyah Sri Utami DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Diyan Herdiyantoro Djuwansah, Muhamad Rachman EDWAN KARDENA Eka Safitri Emma Trinurani Sofyan Enny Ratnaningsih Erni Erni Erni Suminar Eso Solihin Evi Entang Fatimah Evi Entang Fatimah Fadhilla Oktavianingtyas Trisilvi Fajri Syahid Nurhakim Febby Nur Indriani Fera Siti Meilani Fiky Yulianto Wicaksono Fitriatin, Betty Natalie Gordon Pius Marihot Halimah, Ummu ‘Azizah Handayani, Sri Harry Rum Haryadi Ibnu Rizki Perdana Ikrar Nusantara Putra Ikrar Nusantara Putra Ilyas, Ichsan Indra Herliana Iskariman, Ahlan Azman Iva Fitriani Jajang Sauman Hamdani Januar, Dodi Ganjar Jihan Fitria Meilani Khumairah, Fiqriah Hanum Latifah, Tipah Leoni Silvia Lia Nur Linda Maharani, Nadhira Saniya Mahdi Argawan Putra Mahfud Arifin Masruri, Muhammad Kholil Maulana, Nurzen Maya Damayani Maya Damayanti Meddy Rachmadi Mieke Rochimi S Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setyawati Muhammad Agus Mulyana Muhammad Amir Solihin Nadia N. Kamaluddin Nadia Nuraniya Kamaluddin Nanda Aditya Setyawan Nandha Afrilandha NENNY NURLAENY Ninda Meiditia Putri Nizar Ulfah Nugraha, Gita Bina Nugraha, Gita Bina Nugraha, Gita Bina Nurullita Fitri Qurnia Oviyanti Mulyani Pirda Nurhopipah Probo Condrosari Rachelita, Nadia Rainaldi, Rainaldi Rara Rahmatika Risanti Reginawanti Hindersah Reginawanti Hindersah Reginawati Hindersah Rhazista Noviardi Rhazista Noviardi Rhazista Noviardi Richard A. Gunawan Ridha Hudaya Rija Sudirja Rina Devnita Risanti, Rara Rahmatika Risanti, Rara Rahmatika Salsabilla, Camilla Sandra Amalia Riyadi Sandrawati, Apong Santi Rosniawaty Setiawati, Mieke Richimi Setiawati, Mieke Rochimi Shinta Nurrizqi Indrayani Silmi Rahadiana Putri Sumadi Sumadi syafrizal Syafrizal Syafrizal SYARIFUL MUBAROK Syifa Nabila Kurnia Sylvia, Annisa Rosalina Tarra Martiana Dewi Triyani Dewi Tualar Simarmata Tualar Simarmata Ummu ‘Azizah Halimah Uum Umiyati Vera Oktavia Subarja Wati, Dyah Aditya Yori Tridendra Yudhistari Sihombing Yuliati Machfud Yusup Hidayat Zahra Ilmiyati Zellya Handyman Zulkifliani Zulkifliani, Zulkifliani