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EFFECTS OF PETROFILIC MICROORGANISMS AND BULKING AGENT ON HYDROCARBONS BIODEGRADATION EFFICIENCY Zulkifliani; Pujawati Suryatmana; Annisa Rosalina Sylvia; Syafrizal
Scientific Contributions Oil and Gas Vol. 39 No. 3 (2016): SCOG
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/scog.39.3.273

Abstract

Bioremediation is a method which can be used to reduce the amount of pollutants or toxic substances that damage the environment through the use of microorganisms to remove the contaminants. The purpose of this research is to determine the effects of petrofilic microorganisms and bulking agent on the enhancement of hydrocarbons biodegradation efficiency, soil water content, growth rate of petrofilic bacteria and Azotobacter vinelandii. Nine treatments in this research were a petrofilic consortium (Pseudomonas spp., Actinomycetes sp., and petrofilic fungi) and Azotobacter vinelandii (biosurfactan), as well as bulking agent (rice husk charcoal and baglog waste of Oyster Mushrooms). The experiment design used was a randomized block design with three replications, so there were 27 experimental units (microscosmos). The result was that the application of petrofilic microorganisms and bulking agent is the best treatment to enhance the efficiency of hydrocarbons biodegradation, the growth rate of petrofilic bacteria, and soil water content. The petrofilic consortium treatment with the highest value of biodegradation efficiency reached 83,9%.
Enhancing microbial population and biomass of water spinach grown in tailing and inceptisols by manure amendment Hindersah, Reginawanti; Suryatmana, Pujawati; Herdiyantoro, Diyan; Hamdani, Jajang Sauman
Jurnal AGRO Vol 11, No 2 (2024)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/39611

Abstract

The impact of tailings accumulated on agricultural land is the loss of soil profile and decreased soil quality, making plants difficult to grow. This study aimed to observe the effect of cow dung manure (CM) doses to gold mine tailings on total fungal and bacterial populations of soil surrounding roots and water spinach biomass and to analyze the correlation between fungal and bacterial populations with water spinach growth parameters. The experiment was designed in a randomized block design with five treatments and five replications. The treatments included without CM (control) and 5, 10, 15, and 20% of CM in tailing. Similar treatments were added to plants grown in mineral soil, i.e. Inceptisols. The results determined the retarded plant growth in tailing compared to that in Inceptisols. The plant grown in tailing was more responsive to manure amendment. The CM increased total fungal and bacterial populations in the soil around the roots, plant height, leaf number, stem thickness, wet weight, and dry weight of intact plants. Applying 5% of CM caused better growth of water spinach than other treatments. Total fungal and bacterial populations were strongly correlated with water spinach height and dry weight. ABSTRAK Dampak negatif penumpukan tailing di lahan pertanian adalah hilangnya profil tanah dan penurunan kualitas tanah sehingga tanaman sulit tumbuh. Penelitian ini bertujuan untuk mengobservasi pengaruh pemberian dosis pupuk kotoran sapi (PKS) pada tailing tambang emas terhadap populasi jamur dan bakteri total biomassa kangkung darat (Ipomoea reptans (L.) Poir.) serta menganalisis korelasi antara populasi jamur dan bakteri di tanah sekitar perakaran dengan parameter pertumbuhan kangkung. Percobaan pot di rumah kaca disusun dalam rancangan acak kelompok dengan lima perlakuan dan lima ulangan. Perlakuan percobaan adalah tanpa dan dengan penambahan 5, 10, 15 dan 20% PKS ke dalam tailing. Perlakuan yang sama diberikan pada tanaman kangkung dengan tanah Inceptisol. Hasil percobaan menunjukkan bahwa pertumbuhan kangkung di tailing terhambat dibandingkan di tanah Inceptisols, tetapi tanaman di tailing lebih responsif terhadap aplikasi PKS. Pupuk kotoran sapi mampu meningkatkan populasi jamur dan bakteri total di sekitar perakaran, tinggi tanaman, jumlah daun, ketebalan batang, bobot basah serta bobot kering tanaman di tailing. Pemberian 5% PKS lebih meningkatkan pertumbuhan tanaman kangkung dibandingkan dengan perlakuan lainnya. Populasi jamur dan bakteri masing-masing berkorelasi positif dengan hubungan yang sangat kuat dengan bobot kering serta tinggi tanaman kangkung. Percobaan ini menjelaskan bahwa bahan organik penting untuk memperbaiki kualitas tailing dan pertumbuhan tanaman.
AZOTOBACTER AND CHEMICAL FERTILIZER FOR IMPROVING GROUNDNUT YIELD IN FIELD EXPERIMENT Reginawanti Hindersah; Agung Muhammad Yusuf; Dewikusuma Ikhsani Adhiningtyas; Diyan Herdiyantoro; Oviyanti Mulyani; Pujawati Suryatmana
Jurnal Agrotek Tropika Vol 12, No 4 (2024): JURNAL AGROTEK TROPIKA VOL 12, NOVEMBER 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i4.8395

Abstract

Biofertilizer inoculation is a novel and sustainable approach for reducing chemical fertilizer dose. The nitrogen-fixing Azotobacter is a prominent rhizobacteria used in biofertilizer formulation. A field experiment was aimed to observe the N availability, N uptake, nodulation, and growth and yield of groundnuts grown with A. chroococcum liquid inoculant and reduced dose of NPK fertilizer. The field experiment was arranged in a Randomized Block Design to test eight combination treatments of A. chroococcum inoculant doses (2 and 3 L/ha) and NPK fertilizer doses (75, 150 and 300 kg/ha). Azotobacter and NPK application did not affect plant height at two and three weeks after sowing. Groundnuts treated with 2 L/ha Azotobacter combined with 150 kg/ha NPK had more NO3- in soil, N uptake, and root dry weight; its pod number and weight per plot increased 110% and 40% than that in plot received 2L/ha Azotobacter and 300 kg/ha NPK respectively. The experiment found that A. chroococcum shall replace part of NPK fertilizer for growing groundnut in Inceptisols soil order. 
Efek Aplikasi Pupuk NPK cair terhadap P-tersedia, Serapan P, dan Bobot Umbi Bawang Merah (Allium ascalonicum L.) di Tanah Inceptisol Jatinangor Setiawati, Mieke Rochimi; Maharani, Nadhira Saniya; Suryatmana, Pujawati; Hindersah, Reginawanti; Fitriatin, Betty Natalie; Chotimah, Andina; Aditya, Fasa
Soilrens Vol 22, No 2 (2024)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v22i2.61717

Abstract

The increase in corn plant growth is greatly influenced by cultivation techniques includingfertilization. However, the use of inorganic fertilizers for a long period of time can reduce soil quality.An alternative environmentally friendly technology that can substitute for the use of inorganicfertilizers is the biological fertilizer of nitrogen-fixing endotypic bacteria. These endophytic bacteriahave benefits as growth stimulants for plants in increasing nitrogen supply through N2 fixationthrough the air. This study aims to increase the growth of corn plants through the application methodand type of nitrogen-fixing endophytic bacteria. This study used a Randomized Block Design (RBD).The treatment of types and application methods consisted of treatments without endophytic bacterialisolates, endophytic bacterial isolates Gluconacetobacter diazotrophicus Corrig, G. diazotrophicusstrain 4L, and Burkholderia gladioli with three application methods, namely seed treatment, soiltreatment, and their combinations. The results of the study showed that G. diazotrophicus 4L bacteriaapplied by seed and soil treatment gave the best effect on the growth of corn plants on the parametersof the endophytic bacterial population in leaf tissue, wet weight and dry weight of corn by 1 and 3times compared to the control and there was also a tendency to increase the dry and wet weight of thecorn plant crown
Respon Tanaman Jagung terhadap Cara Aplikasi dan Jenis Bakteri Endofitik Penambat Nitrogen Setiawati, Mieke Rochimi; Maharani, Nadhira Saniya; Suryatmana, Pujawati; Hindersah, Reginawanti; Fitriatin, Betty Natalie; Chotimah, Andina; Aditya, Fasa
Soilrens Vol 22, No 2 (2024)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v22i2.64415

Abstract

The increase in corn plant growth is greatly influenced by cultivation techniques includingfertilization. However, the use of inorganic fertilizers for a long period of time can reduce soil quality.An alternative environmentally friendly technology that can substitute for the use of inorganicfertilizers is the biological fertilizer of nitrogen-fixing endotypic bacteria. These endophytic bacteriahave benefits as growth stimulants for plants in increasing nitrogen supply through N2 fixationthrough the air. This study aims to increase the growth of corn plants through the application methodand type of nitrogen-fixing endophytic bacteria. This study used a Randomized Block Design (RBD).The treatment of types and application methods consisted of treatments without endophytic bacterialisolates, endophytic bacterial isolates Gluconacetobacter diazotrophicus Corrig, G. diazotrophicusstrain 4L, and Burkholderia gladioli with three application methods, namely seed treatment, soiltreatment, and their combinations. The results of the study showed that G. diazotrophicus 4L bacteriaapplied by seed and soil treatment gave the best effect on the growth of corn plants on the parametersof the endophytic bacterial population in leaf tissue, wet weight and dry weight of corn by 1 and 3times compared to the control and there was also a tendency to increase the dry and wet weight of thecorn plant crown.
Enhancing Tomato (Lycopersicon esculentum Mill.) Growth in a Green House Using NPK Fertilizer Coated with Endospore-Forming Bacillus Hindersah, Reginawanti; Fitriatin, Betty Natalie; Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Risanti, Rara Rahmatika; Dewi, Yeni Wispa
Agrikultura Vol 36, No 1 (2025): April, 2025
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v36i1.62354

Abstract

Long-term use of NPK composite fertilizers mixed with biofertilizers, including spore-forming Bacillus rhizobacteria, can improve plant quality and growth. This study aims to analyze Bacillus’s growth and spore production in chemical-liquid growing media and to observe the effects of Bacillus-coated NPK formulas (BCN) on the growth of tomatoes in potted Andisol and the Bacillus population in the rhizosphere. The evaluation of Bacillus growth was conducted in the laboratory whereas the effect of the BCN was conducted as a pot experiment in a Randomized Block Design. The treatments included recommended dosage of NPK 16-16-16, liquid inoculant of Bacillus consortium, BCN-C, and BCN-G formulas with 100%, 75%, and 50% of the recommended dosage, respectively. Control plants were not treated with fertilizer. Laboratory assay verified that Yuniarti’s medium supported Bacillus-consortium growth and produced the highest Bacillus vegetative cells and spores. The BCN application significantly increased plant height, number of leaves, root length, and tomato biomass compared to the control up to 6 weeks after planting. Three-quarters of the dosage of BCN-C and BCN-G gave the best growth of tomatoes. Introducing BCN and conventional NPK did not change the population of Bacillus vegetative and spore form in the rhizosphere. Results indicate that BCN can replace conventional NPK 16-16-16 fertilizer for tomato plants in Andisol soil order.
Yield and Fruits Quality of Potted-Strawberry Grown with Bacillus-Coated NPK Fertilizer Hindersah, Reginawanti; Fitriatin, Betty Natalie; Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Risanti, Rara Rahmatika; Aditya, Fasa; Nugraha, Gita Bina
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.31809

Abstract

NPK fertilizer coated with rhizobacteria Bacillus supports biofertilizer use while reducing the dose of NPK fertilizer. The objective of the greenhouse experiment was to analyze the growth and fruit production of potted strawberries following Bacillus-coated NPK 16-16-16 (BCN). The research was set up in a Completely Randomized Block Design that tested four BCN dosage and type combinations. The control treatment consisted of no fertilizer and complete and a half dosage of NPK fertilizer 16-16-16. The results showed that the shoot height and the fruit number of strawberry cv Festival were not affected by NBB application, but the type of NPK fertilizer affected the fruit weight per plant and sweetness. A complete dosage of BNN formula C produced the most significant fruit weight, but the highest sweetness was obtained from plants treated with half the dose of NPK conventional fertilizer. However, one or half doses of NBB formula G reduced the fruit sweetness. This experiment shows the potential of NBB to support strawberry production in a greenhouse, but a longer duration of the experiment is needed to obtain more harvest data.
Efektifitas Azotobacter chroococcum dalam Proses Bioremediasi Tanah Terkontaminasi Limbah Minyak Bumi Menggunakan Bakteri Petrofilik Suryatmana, Pujawati; Latifah, Tipah; Kamaluddin, Nadia Nuraniya; Hindersah, Reginawanti; Setiawati, Mieke Rochimi
Soilrens Vol 23, No 1 (2025)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v23i1.66113

Abstract

Bioremediation is a method for restoring land contaminated by waste, utilizing the activity of microorganisms to reduce and eliminate the toxicity levels of pollutants. A major challenge in the bioremediation of petroleum waste is the extremely low solubility of petroleum and the limited capacity of hydrocarbon-degrading bacteria. This study aims to examine the potential of petrophilic bacteria and Azotobacter chroococcum in enhancing hydrocarbon degradation efficiency, the viability of petrophilic bacteria and Azotobacter spp., and soil acidity characteristics (pH) during the bioremediation process. The experiment used a factorial randomized complete block design (RCBD) with two factors and three replications. The first factor was the type of petrophilic bacteria which consisted of two levels: (a0) without petrophilic bacteria, and (a1) 2% petrophilic bacterial inoculation per waste load. The second factor was the dose of Azotobacter chroococcum, consisted of four levels: (b0) without A. chroococcum, (b1) 0.5%, (b2) 1%, and (b3) 1.5% A. chroococcum. The results showed that soil pH decreased from a range of 8–9.1 to 6.6–6.7 after the bioremediation process, despite no significant differences were detected between treatments. Biodegradation efficiency increased with the application of A. chroococcum and petrophilic bacteria compared to the control, although no significant differences were found between the bacterial treatments. The application of A. chroococcum at 1% and 1.5% significantly increased Azotobacter population density. Overall, petrophilic bacteria and A. chroococcum demonstrated high effectiveness in improving biodegradation efficiency.
Bacillus-Coated Inorganic Fertilizer to Improve Plant Growth and Bacillus Count in the Rhizosphere of Broccoli Hindersah, Reginawanti; Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Fitriatin, Betty Natalie; Chotimah, Andina; Dewi, Yeni Wispa; Risanti, Rara Rahmatika
Soilrens Vol 23, No 1 (2025)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v23i1.66092

Abstract

Inovasi pupuk Bacillus pembentuk endospora berlapis NPK diharapkan dapat mengatasi rendahnya ketersediaan fosfat pada tanah Andisol. Telah dilakukan percobaan pot untukmenganalisis dampak berbagai dosis dua jenis BCN terhadap pertumbuhan dan biomassa brokoli,serta terhadap populasi Bacillus di rizosfernya. Percobaan disusun dalam rancangan blok acak untukmenguji kombinasi jenis dan dosis BCN dibandingkan dengan NPK dan inokulan cair Bacillus. Tidakada kombinasi jenis dan dosis BCN yang mempengaruhi pertumbuhan brokoli umur 6 minggu,meskipun 100% BCN-B meningkatkan tinggi tanaman pada 2 minggu. Biomassa akar meningkatsetelah aplikasi 75% BCN-A, sedangkan 100% BCN-B meningkatkan biomassa pucuk. Populasi sporameningkat pada rizosfir tanaman dengan 100% BCN-B dan 75% dari kedua jenis BCN namun totalpopulasi sel vegetatif tidak dipengaruhi oleh BCN. Percobaan ini menyarankan penggunaan BCNuntuk meningkatkan pertumbuhan brokoli.
Potensi Berbagai Jenis Kompos Produk Samping Pertanian dalam Meningkatkan Populasi Azotobacter, C-Organik, N-Total Tanah, dan Hasil Tanaman Tomat pada Inceptisols Setiawati, Mieke Rochimi; Iskariman, Ahlan Azman; Suryatmana, Pujawati; Kamaluddin, Nadia Nuraniya
Soilrens Vol 23, No 1 (2025)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v23i1.66076

Abstract

Inceptisols hs a potential to be developed as a growth medium for tomato cultivation. The low to moderate fertility of Inceptisols can be improved by applying compost derived from various types ofagricultural waste, which can increase soil organic matter content, support the growth of nitrogenfixingrhizosphere microbes such as Azotobacter sp., enhance soil fertility, and ultimately improvetomato yield. This study aimed to analyze the effects of different types and application dosage ofagricultural waste compost on the population of Azotobacter sp., soil organic carbon, total nitrogen,and the yield of tomato plants (Lycopersicum esculentum Mill.) grown on Inceptisols. A greenhousepot experiment was conducted with a Randomized Complete Block Design with ten treatments andthree replication, consisted of control (no compost) and three types of compost (rice straw, sugarcanebagasse, and empty oil palm fruit bunches) applied at rates of 10, 15, and 20 tons ha⁻¹. Theexperimenal result showed that agricultural waste compost significantly increased the population ofAzotobacter sp., soil organic carbon content, and tomato yield, but had no significant effect on totalsoil nitrogen. The application of rice straw compost at a dosage of 20 tons ha⁻¹ was the most effectivetreatment to increase Azotobacter sp. population, fruit weight, and the number of tomato fruits perplant.
Co-Authors . WISJNUPRAPTO Adawiah, Aliya Zahrah Ade Setiawan Aditya, Fasa Aditya, Fasa Aditya, Fasa Agung Karuniawan Agung Karuniawan Agung Muhammad Yusuf Aliya Zahrah Adawiah Alyani Shabrina Amalia Chusnul Anas Ramdhani Andi Hana Mufidah Elmirasari ANNE NURAINI Anne Nurbaity Anne Nurbaity ANNE NURBAITY Anni Yuniarti Annisa Rosalina Sylvia Apong Sandrawati Asmiran, Priyanka Aten Komarya Bagus Adityo Bang, Sunbaek Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Budiman, Muhammad Nafariz Chotimah, Andina Christine Angel Damayani, Maya Dedeh H. Arief Dedi Ruswandi Denny Sobardini Sobarna Denny Sobardini Sobarna Dewi, Yeni Wispa Dewikusuma Ikhsani Adhiningtyas Diyah Sri Utami DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Diyan Herdiyantoro Djuwansah, Muhamad Rachman EDWAN KARDENA Eka Safitri Emma Trinurani Sofyan Enny Ratnaningsih Erni Erni Erni Suminar Eso Solihin Evi Entang Fatimah Evi Entang Fatimah Fadhilla Oktavianingtyas Trisilvi Fajri Syahid Nurhakim Febby Nur Indriani Fera Siti Meilani Fiky Yulianto Wicaksono Fitriatin, Betty Natalie Gordon Pius Marihot Halimah, Ummu ‘Azizah Handayani, Sri Harry Rum Haryadi Ibnu Rizki Perdana Ikrar Nusantara Putra Ikrar Nusantara Putra Ilyas, Ichsan Indra Herliana Iskariman, Ahlan Azman Iva Fitriani Jajang Sauman Hamdani Januar, Dodi Ganjar Jihan Fitria Meilani Khumairah, Fiqriah Hanum Latifah, Tipah Leoni Silvia Lia Nur Linda Maharani, Nadhira Saniya Mahdi Argawan Putra Mahfud Arifin Masruri, Muhammad Kholil Maulana, Nurzen Maya Damayani Maya Damayanti Meddy Rachmadi Mieke Rochimi S Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setyawati Muhammad Agus Mulyana Muhammad Amir Solihin Nadia N. Kamaluddin Nadia Nuraniya Kamaluddin Nanda Aditya Setyawan Nandha Afrilandha NENNY NURLAENY Ninda Meiditia Putri Nizar Ulfah Nugraha, Gita Bina Nugraha, Gita Bina Nugraha, Gita Bina Nurullita Fitri Qurnia Oviyanti Mulyani Pirda Nurhopipah Probo Condrosari Rachelita, Nadia Rainaldi, Rainaldi Rara Rahmatika Risanti Reginawanti Hindersah Reginawanti Hindersah Reginawati Hindersah Rhazista Noviardi Rhazista Noviardi Rhazista Noviardi Richard A. Gunawan Ridha Hudaya Rija Sudirja Rina Devnita Risanti, Rara Rahmatika Risanti, Rara Rahmatika Salsabilla, Camilla Sandra Amalia Riyadi Sandrawati, Apong Santi Rosniawaty Setiawati, Mieke Richimi Setiawati, Mieke Rochimi Shinta Nurrizqi Indrayani Silmi Rahadiana Putri Sumadi Sumadi syafrizal Syafrizal Syafrizal SYARIFUL MUBAROK Syifa Nabila Kurnia Sylvia, Annisa Rosalina Tarra Martiana Dewi Triyani Dewi Tualar Simarmata Tualar Simarmata Ummu ‘Azizah Halimah Uum Umiyati Vera Oktavia Subarja Wati, Dyah Aditya Yori Tridendra Yudhistari Sihombing Yuliati Machfud Yusup Hidayat Zahra Ilmiyati Zellya Handyman Zulkifliani Zulkifliani, Zulkifliani