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Journal : BIOGENESIS

PENGARUH PEMBERIAN GIBERELIN PADA MEDIA VACIN DAN WENT TERHADAP PERKECAMBAHAN BIJI ANGGERIK BULAN (Phalaenopsis amabilis Bl) SECARA IN VITRO Yusnida Bey; Wan Syafii; Nur Ngafifah
Biogenesis Vol 1, No 2 (2005)
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.283 KB) | DOI: 10.31258/biogenesis.1.2.57-61

Abstract

In vitro investigation has conducted in order to find out the respons of orchid (Phalaenopsis amabilis BI) in Vacin and Went media by Gibberellins treatment, this research was carry out at Balai Benih Tanaman Pangan dan Holtikultura Pekanbaru during June until August 2003 by using completely Random Design through 5 treatment and 4 replication. The treatment were Gibberellins (ppm): 0, 2, 4, 6 and 8. The parameter were observed i.e ; the emergence timing of protocorm like bodies, seedling height, and seedling biomass. Observated data were analyzed descriptifely and analysis of variance with Duncan Multiple Range Test at 5% level of significance. The results shows that the 2 ppm GA3 treatment have a good effect on the emergence timing of protocorm like bodies (13-16 days after planting), average of seedling height (1,5 cm) and seedling biomass (1,18 gr). We concluded that the timing of orchid seedling emergence could be accelerated by increasing the concentration GA3 treatment until 2 ppm.     Key words:  Seed of orchid, Gibberellins,  In vitro, Germination, Phalaenopsis amabilis Bl
RESPON EKSPLAN DAUN TANAMAN JERUK MANIS (Citrus sinensis L.) SECARA IN VITRO AKIBAT PEMBERIAN NAA DAN BA Sri Wulandari; Wan Syafii; Yossilia '
Biogenesis Vol 1, No 01 (2004)
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1261.382 KB) | DOI: 10.31258/biogenesis.1.01.21-25

Abstract

The effect of Naphthalene Acetic Acid (NAA) and Benzyl Adenine (BA) of concentration of leaf eksplan Citrus sinensis L.  by in vitro was studied at tissue culture laboratory of Balai Benih Induk Pekanbaru, The experiment was conducted using Completely Randomized Design (RAL) with three replicates.  The treatment consist of A (without NAA and BA), B (0,1 ppm NAA and 0,1 ppm BA), C (1 ppm NAA and 0,1  ppm BA), D (10 ppm NA and 0,1 ppm BA), E (0,1 ppm NAA and 1 ppm BA). F (1 ppm NAA and 1 ppm BA). G (10 ppm NAA and  1 ppm BA), H (0,1  ppm NAA and  10 ppm BA), I (1 ppm NAA and 10 ppm BA) and J (10 ppm NAA and 10 ppm BA),The results of this experiment showed that respond growth of good leaf eksplan.  NAA and BA at concentration  0,1;  1 and  10 ppm can yield forming o f root and callus can’t forming of buds. Callus initiation and root quickest of concentration  10 ppm NAA and 0,1 ; 1; 10 BA ppm that is  10,00 and  14,66.   Amount    root o f concentration  1 ppm NAA and  10 ppm BA is 29,66 and wet weight of callus high at gave of concentration 10 ppm NA and 10 ppm BA is 0,25 gr.   Key words : Citrus Sinensis L, BA, Eksplan, NAA