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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Akademika Biologi Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) International Journal of Agriculture System Indonesian Journal of Forestry Research
Teuku Tajuddin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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The Graded Concentration of Bleach Solution and Material Size Plays an Important Role in the In Vitro Sterilization of Thyphonium flagelliforme Plants Rahmadara, Gemilang; Tajuddin, Teuku; Suparjo, Suparjo; Sobir, Sobir; Dinarti, Diny; Khairiyah, Hayat
International Journal of Agriculture System VOLUME 12 ISSUE 1, JUNE 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v12i1.5070

Abstract

The sterilization is a primary step in in-vitro culture. plant breeding through in-vitro culture hinges on the successful effectiveness of the sterilization method in producing sterile plants. This research aims to optimize sterilization methods using a completely randomized factorial design. The first factor is the size of the material (small and large), and the second factor is the graded concentration of the bleach solution. The study revealed that Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants. The application of graded bleach solution concentrations at 25%, 20%, and 15% is able to reduce contamination to 0% on small size materials and 5 % on large size materials. Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants.
Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach: Identification of Gene Candidates in Diterpenoid Fadhullah, Hafizh; Purwoko, Devit; Zulaeha, Siti; Hanifah, Nurul Fitri; Hartuti, Endah Dwi; Rahmadara, Gemilang; Safarrida, Anna; Reninta, Rikania; Evawati, Evawati; Roza, Irwan; Tajuddin, Teuku
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.08

Abstract

Curcuma longa is a medicinal plant renowned for its therapeutic properties and potential treatment of cancer. This study focused on the biosynthesis of diterpenoids in the rhizome and leaves of C. longa. The genes responsible for producing these medicinal compounds were analyzed using BLASTx, Gene Ontology (GO) annotation, differential expression, and homology. The substantial dataset was obtained from the National Center for Biotechnology Information (NCBI), comprising 151,730,334 clean reads and 167,264 transcripts for the analysis. The results of the BLASTx analysis were as follows: NR yielded 65.93%, Swiss-Prot yielded 44.52%, and COG yielded 17.35%. Subsequently, GO annotation was performed using Blast2GO, resulting in an annotation rate of 56.79%. Differential expression analysis revealed a total of 636 genes that were significantly differentiated between the rhizome and leaves. The homology analysis resulted in 11 proteins associated with diterpenoid biosynthesis and nine proteins related to CYP450. Approximately three class I proteins were highly expressed in the rhizome. Additionally, seven CYP450 enzymes from the CYP71D and CYP726 subfamilies were identified; three were highly expressed in the rhizome. The expression patterns of these enzymes were similar to the aforementioned three class I diTPSs, indicating their potential involvement in macroditerpenoid biosynthesis in C. longa. These findings provide valuable genomic resources for future functional genomics research on C. longa, facilitating targeted efforts to enhance the production of bioactive compounds.
The Graded Concentration of Bleach Solution and Material Size Plays an Important Role in the In Vitro Sterilization of Thyphonium flagelliforme Plants Rahmadara, Gemilang; Tajuddin, Teuku; Suparjo, Suparjo; Sobir, Sobir; Dinarti, Diny; Khairiyah, Hayat
International Journal of Agriculture System VOLUME 12 ISSUE 1, JUNE 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v12i1.5070

Abstract

The sterilization is a primary step in in-vitro culture. plant breeding through in-vitro culture hinges on the successful effectiveness of the sterilization method in producing sterile plants. This research aims to optimize sterilization methods using a completely randomized factorial design. The first factor is the size of the material (small and large), and the second factor is the graded concentration of the bleach solution. The study revealed that Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants. The application of graded bleach solution concentrations at 25%, 20%, and 15% is able to reduce contamination to 0% on small size materials and 5 % on large size materials. Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants.
Analysis of Quantitative Trait Loci for Protein Content in Soybean Seeds using Recombinant Inbred Lines Tajuddin, Teuku
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 1 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.943 KB) | DOI: 10.24831/jai.v33i1.1511

Abstract

Protein content in the seed is quantitatively inherited and controlled by polygene.  The quality of seed protein content has been studied extensively, however, information on its quantity is still limited.  In order to analyze the genetic basis of these traits, recombinant inbred lines (RILs) derived from a cross between Glycine max (L.) Merrill variety Misuzudaizu and variety Moshidou Gong 503 were planted in two environments and evaluated for seed protein content.  The broad sense heritability of the traits ranged from 0.74 to 0.79 in our RIL population.  Single-factor analysis of variance, interval mapping and composite interval mapping were used to detect significant associations between traits and genetic markers.  A total of 10 QTLs, which were significant in at least one environment were identified.  Each QTL explained the total phenotypic variation for protein content in the range from 3.4% to 29.7%.  Among all the detected QTLs, three of them were detected in both environments.  QTLs identified in this study were mapped in the soybean linkage map.  The results obtained in our study may serve as a base for analyzing the genetic control of protein content and may eventually enable to change the seed constituents.   Key words: Glycine max (L.) Merrill, quantitative trait loci, protein, recombinant inbred lines.
THE EFFECTIVENESS OF COLCHICINE AND ORYZALIN ON POLYPLOIDY INDUCTION IN TEAK (Tectona grandis Linn. f.) IN VITRO Fauzan, Yusuf Sigit A.; Supriyanto; Mulyono, Joko; Tajuddin, Teuku
Indonesian Journal of Forestry Research Vol. 11 No. 1 (2024): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2024.11.1.1-15

Abstract

Indonesian government has launched a community plantation forests program that would be beneficial for remedying shortage in domestic demand for teak wood every year. This program needs to be supported by the availability of quality seeds resources and quality seedlings utilizing polyploid teak (Tectona grandis). Our study aimed to examine the effectiveness of colchicine and oryzalin to modify diploid into polyploid T. grandis based on growth response, morphological, anatomical and cytological alteration, as well as the acclimatization ability of polyploid plantlets. The materials used were aseptic lateral shoots consisting of nodal segments which were immersed in antimitotic agents at the concentration of 0, 15 and 30 μM for 5 days, then cultured on regeneration medium until the 8 weeks and followed by acclimatization. The results showed that colchicine at the concentration of 30 μM was the most effective to induce polyploidy of plantlets in the parameter of high growth rate, the length of internodes, the number of leaf plantlets and increase the leaf surface area and the significant chlorophyll index content compared to the control. Anatomical analysis of polyploid was characterized by increasing the leaf thickness, stomata size, decreased stomatal density and increased chloroplast content in guard cells. Based on cytological examination on polyploidy plantlet, there was an increased in the number of chromosomes in the cell nucleus. The acclimatization of polyploid successfully induced rooting and a 100% survival rate of grown plantlets. Polyploid seedlings were able to grow and well adapted to the new environment of acclimatization
Co-Authors . Suparjo, . . Supriyanto Abd. Rasyid Syamsuri AGUS SUYONO Ahmad Riyadi Andi Rochandi Anna Safarrida, Anna Anto Budiharjo Bedah Rupaedah, Bedah Cartealy, Imam Cartealy, Imam Cartealy, Imam Civi Cartealy, Imam Civi damayanti, dini Devit Purwoko Diny Dinarti Erwinda . Evawati Evawati Fadhullah, Hafizh Farida Rosana Mira, Farida Rosana Fauzan, Yusuf Sigit A. Fauzan, Yusuf Sigit Ahmad Fauzan, Yusuf Sigit Ahmad Gemilang Rahmadara Hanifah, Nurul Fitri Hartuti, Endah Dwi Hermin Pancasakti Kusumaningrum Irwan Roza Joko Mulyono Juanda ., Juanda Juwartina Ida Royani, Juwartina Ida Karyanti ., Karyanti Karyanti Karyanti, Karyanti Karyanti, . Karyanti, . Khairiyah, Hayat Khairiyah, Hayat Kitagawa, Sayuri Mahmud Sugianto, Mahmud MEITY SURADJI SINAGA Minaldi ., Minaldi Nadirman Haska Nurul Fitri Hanifah Pramudyawardhani, Okky Dwi Pramudyawardhani, Okky Dwi purwoko, devit Purwoko, Devit Rahmadara, Gemilang Reninta, Rikania Rismayanti Rismayanti Rudiyana, Yayan Saga, Hirohisa Siti Zulaeha, Siti Sobir Sobir Sri Wilarso Budi Sudarsono Sudirman Yahya Sudjai, Imam Suharsono Suharsono Suparjo Suparningtyas, Juniza Firdha Supriyanto SYOFI ROSMALAWATI Tati Sukarnih, Tati Wahono Sumaryono YENNI BAKHTIAR Yohanes Chandrawijaya Yusuf Sigit, Yusuf