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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Akademika Biologi Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) International Journal of Agriculture System Indonesian Journal of Forestry Research

Teuku Tajuddin

Unknown Affiliation

Author-ID : 400191

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology

Published : 55 Documents Claim Missing Document

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Preface JBBI Vol 3, No 1, June 2016: Foreword and Acknowledgement Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Preface JBBI Vol 4, No 2, December 2017: Foreword and Acknowledgement Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 2 (2017): December 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Preface JBBI Vol 5, No 1, June 2018: Foreword and Acknowledgement Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Preface JBBI Vol 5, No 2, December 2018: Foreword and Acknowledgement Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

PELACAKAN FRAGMEN GEN PENYANDI ENZIM ß-KETOASIL-ACP SINTASE II (KAS II) DARI MESOKARP KELAPA SAWIT (ELAEIS GUINEENSIS JACQ. L.) Yohanes Chandrawijaya; Teuku Tajuddin; Hermin Pancasakti Kusumaningrum; Anto Budiharjo
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.861 KB)

The standard of quality is one of the determining values of crude palm oil as an international trade commodity. Better standard of quality for crude palm oil is a constant demand of the market. Quality improvement can be made by increasing the contents of oleic acid in the mesocarp of E. guinensis. Among the uses of oleic acid are as follows: anti-carcinogenic agent, anti-oxidant, source of pro-vitamin A, and source of Vitamin E. Oleic acid is a form of non-saturated fatty acid encoded by KAS II genes. The expression profiling of KAS II is achieved through isolation of total RNA by Trizol reagent, RNA purification, using DNAse RNAse free, synthesis of cDNA using Reverse Transcriptation PCR approach, and amplification of KAS II genes with Nested PCR approach. The amplification process of KAS II genes is carried out using both internal and external primers. The first step of the external primer PCR is F-KAS-1 and R-KAS-1. Internal primer of PCR in the second step is F-KAS-2 and R-KAS-2. The results of this research are fragments of KAS II genes between 1500–2000 bp. These amplicons are suitable with primers designed at the approximation of 1796 bp. Selection of three amplicons at the annealing temperatures of 54oC, 55.9oC, and 58oC shows good DNA band visualizations. Annealing at 58oC shows the best result with high intensity DNA band and no smear. Further research is needed to determine the accuracy of the amplicons through sequencing step. Keywords: KAS II, Elaeis guineensis, annealing, Nested PCR, RT-PCR

Isolation and Identification of Mycorrhizosphere Bacteria and Their Antagonistic Effects Towards Ganoderma boninense in vitro YENNI BAKHTIAR; SUDIRMAN YAHYA; WAHONO SUMARYONO; MEITY SURADJI SINAGA; SRI WILARSO BUDI; TEUKU TAJUDDIN
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Basal stem rot caused by Ganoderma boninense is the most serious disease of oil palm (Elaeis guineensis) in Indonesia and it has caused major loss in palm oil production. Mycorrhizosphere bacteria offer possible advantages as biocontrol agents as they live and proliferate together with arbuscular mycorrhizal fungi, which have an ability to increase plant resistance against pathogens. A study was conducted to isolate mycorrhizosphere bacteria from spores of arbuscular mycorrhizal fungi and test their antagonistic effects against G. boninense in vitro. All bacterial isolates were identified based on 16S rDNA analysis and it revealed that eleven out of twenty mycorrhizosphere bacteria isolated were related to Bacillus with similarity ranging from 97 to 100%, whereas other isolates were identified as Pseudomonas, Streptomyces, Kocuria, Enterobacter, Brevundimonas, and Alcaligenes with similarities ranging from 96 to 100%. Fourteen out of twenty mycorrhizosphere bacteria showed a varying degree of inhibition towards the growth of G. boninense in vitro. Of these, isolate B10 (closely related to Bacillus subtilis ZJ06) showed the highest inhibitory effect followed by B17 (closely related to Bacillus subtilis N43). Therefore, these bacteria have a potential to be used as biocontrol agents to control basal stem rot disease caused by G. boninense in oil palm.

Comparison of DNA Isolation Methods that Derived from Leaves of a Potential Anti-Cancer Rodent Tuber (Typhonium flagelliforme) Plant Gemilang Rahmadara; Nurul Fitri Hanifah; Rismayanti Rismayanti; Devit Purwoko; Andi Rochandi; Teuku Tajuddin
International Journal of Agriculture System VOLUME 10 ISSUE 2, DECEMBER 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v10i2.3966

The content of polysaccharides, polyphenols, proteins, and RNA compounds is the main problem often found in Plants DNA isolation, which inhibit the process of DNA isolation. Comparing the methods of plant DNA isolation is necessary for obtaining the DNA with good quality, purity, high concentration and efficiency time and cost. This study aimed to determine the best DNA isolation method that derived from leaves of a potential anti-cancer Rodent Tuber (Typhonium flagelliforme) plant by comparing the conventional DNA isolation method (cetyl trimethyl ammonium bromide/CTAB) and 2 commercial kits (Promega Wizard™ Genomic DNA Purification Kits, and Geneaid Genomic Mini Kit). The results showed that the CTAB method yielded a higher amount of DNA (>100 ng/µL) at the cost of 0.49 USD per sample, in comparison with Promega method (69.19 to 157.68 ng/µL) at 3.28 USD per sample and Geneaid method (8.15 to 18.52 ng/µL) at 2.06 USD per sample. Based on the purity of isolated DNA (A260/280), CTAB method produced relatively similar DNA quality to Promega kit (1.8-2.0). On the other hand, Geneaid method resulted in a lower purity value at 1.15 to 1.60.

Appendix JBBI Vol 9, No 2, December 2022: Keyword Index and Author Index Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Preface JBBI Vol 9, No 2, December 2022: Foreword and Acknowledgement Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.505 KB)

Identification of Gene Candidates in Diterpenoid Biosynthesis of Curcuma longa: An mRNA Sequencing Approach: Identification of Gene Candidates in Diterpenoid Fadhullah, Hafizh; Purwoko, Devit; Zulaeha, Siti; Hanifah, Nurul Fitri; Hartuti, Endah Dwi; Rahmadara, Gemilang; Safarrida, Anna; Reninta, Rikania; Evawati, Evawati; Roza, Irwan; Tajuddin, Teuku
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.08

Curcuma longa is a medicinal plant renowned for its therapeutic properties and potential treatment of cancer. This study focused on the biosynthesis of diterpenoids in the rhizome and leaves of C. longa. The genes responsible for producing these medicinal compounds were analyzed using BLASTx, Gene Ontology (GO) annotation, differential expression, and homology. The substantial dataset was obtained from the National Center for Biotechnology Information (NCBI), comprising 151,730,334 clean reads and 167,264 transcripts for the analysis. The results of the BLASTx analysis were as follows: NR yielded 65.93%, Swiss-Prot yielded 44.52%, and COG yielded 17.35%. Subsequently, GO annotation was performed using Blast2GO, resulting in an annotation rate of 56.79%. Differential expression analysis revealed a total of 636 genes that were significantly differentiated between the rhizome and leaves. The homology analysis resulted in 11 proteins associated with diterpenoid biosynthesis and nine proteins related to CYP450. Approximately three class I proteins were highly expressed in the rhizome. Additionally, seven CYP450 enzymes from the CYP71D and CYP726 subfamilies were identified; three were highly expressed in the rhizome. The expression patterns of these enzymes were similar to the aforementioned three class I diTPSs, indicating their potential involvement in macroditerpenoid biosynthesis in C. longa. These findings provide valuable genomic resources for future functional genomics research on C. longa, facilitating targeted efforts to enhance the production of bioactive compounds.

Co-Authors . Suparjo, . . Supriyanto Abd. Rasyid Syamsuri AGUS SUYONO Ahmad Riyadi Andi Rochandi Anna Safarrida, Anna Anto Budiharjo Bedah Rupaedah, Bedah Cartealy, Imam Cartealy, Imam Cartealy, Imam Civi Cartealy, Imam Civi damayanti, dini Devit Purwoko Diny Dinarti Erwinda . Evawati Evawati Fadhullah, Hafizh Farida Rosana Mira, Farida Rosana Fauzan, Yusuf Sigit A. Fauzan, Yusuf Sigit Ahmad Fauzan, Yusuf Sigit Ahmad Gemilang Rahmadara Hanifah, Nurul Fitri Hartuti, Endah Dwi Hermin Pancasakti Kusumaningrum Irwan Roza Joko Mulyono Juanda ., Juanda Juwartina Ida Royani, Juwartina Ida Karyanti ., Karyanti Karyanti Karyanti, Karyanti Karyanti, . Karyanti, . Khairiyah, Hayat Khairiyah, Hayat Kitagawa, Sayuri Mahmud Sugianto, Mahmud MEITY SURADJI SINAGA Minaldi ., Minaldi Nadirman Haska Nurul Fitri Hanifah Pramudyawardhani, Okky Dwi Pramudyawardhani, Okky Dwi purwoko, devit Purwoko, Devit Rahmadara, Gemilang Reninta, Rikania Rismayanti Rismayanti Rudiyana, Yayan Saga, Hirohisa Siti Zulaeha, Siti Sobir Sobir Sri Wilarso Budi Sudarsono Sudirman Yahya Sudjai, Imam Suharsono Suharsono Suparjo Suparningtyas, Juniza Firdha Supriyanto SYOFI ROSMALAWATI Tati Sukarnih, Tati Wahono Sumaryono YENNI BAKHTIAR Yohanes Chandrawijaya Yusuf Sigit, Yusuf

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