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All Journal Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Jurnal Edukasi dan Sains Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Devotion: Journal of Research and Community Service JESBIO : Jurnal Edukasi dan Sains Biologi Biofaal Journal Jurnal Kedokteran Hewan Rumphius Pattimura Biological Journal Kalwedo Sains (KASA)
Adrien Jems Akiles Unitly
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam (FMIPA) Universitas Pattimura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Veterinary

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Journal : Rumphius Pattimura Biological Journal

 1 
ESTRUS CYCLE OF Rattus norvegicus EXPOSED TO CIGARETTE SMOKE AFTER TREATMENT ETHANOL EXTRACT OF GRASS (Biophytum petersianum Klotzsch) Simatauw, Anniestasya Z; Unitly, Adrien Jems Akiles
RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i1p001-007

Abstract

Kebar grass is a plant that contains phytoestrogens which have the same effect as endogenous estrogens which are thought to be able to increase endogenous estrogen levels in the blood due to the adverse effects of free radicals in the form of cigarette smoke. The purpose of this study was to determine the effect of the ethanol extract of kebar grass (Biophytum petersianum Klotzsch) on the length of the estrous cycle of Rattus norvegicus rats exposed to cigarette smoke. The research used an experimental method, namely a completely randomized design with four treatment groups and three replications. Rats the negative group was fed normally, the positive control group was exposed to cigarette smoke for 28 days, while the rats in the Kebar grass ethanol extract dosed group were given cigarette smoke exposure for 28 days, and were gived Kebar grass ethanol extract for 28 days. Vaginal smears were taken in the morning with a span of 24 hours for 28 days. Changes in the vaginal epithelium are examined to determine the phase of the estrous cycle using a microscope. The data obtained were analyzed by Analysis of Variance (ANOVA)-SPSS version 16.0 followed by Duncan test with a 95% confidence interval (α=0.05). The results showed that administration of Kebar grass ethanol extract was able to extend the estrous cycle time at a dose of 0.135 mg/head/day due to prolongation of the proestrus and estrus phases.
THE STUDY OF ADMINISTRATION EXTRACT KEBAR GRASS ON NUMBER LEYDIG CELLS AND SERTOLI CELLS IN RAT (Rattus norvegicus) THAT EXPOSED WITH CIGARETTE SMOKE Unitly, Adrien Jems Akiles
RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i1p008-011

Abstract

This research aimed to study on the microscopic changes of number leydig cells and sertoli cells. A factorial CRD with periode of treatment and sample collection was applied in this study. An exposure of cigarette smoke was carried out at 10 cigarettes/rat/day for 2.5 hours in a smoking chamber. Extract kebar’s grass given was 0.0945 mg/g body weight/day. Twenty seven (27) of male rats were divided into four groups. N group was untreated animals, 20h group is group that was expose to cigarette smoke for 20 days then given administration extract kebar’s grass for 20 days and stopping treatment for 20 days and 60h group is group that was expose to cigarette smoke for 60 days then given administration extract kebar’s grass 60 days and stopping treatment for 60 days. Data collection was carried out at twice that after cigarette smoke exposure (T0), second after administration extract kebar’s grass (T1) and third after stopping treatment (T2). The parameters measured were the number of leydig cells and sertoli cells. That increasing number leydig cells and sertoli cells of rat exposed to cigarette smoke after gived seaweed extract kebar’s grass for 20 days and
ACTIVITY OF CLOVE LEAVES (Syzygium aromaticum L.) LARVACIDES ON MORTALITY OF Anopheles sp. Sapulette, Fenska Violenta; Unitly, Adrien Jems Akiles; Moniharapon, Debby
RUMPHIUS Vol 1 No 2 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i2p042-046

Abstract

This study used a completely randomized design with a sample of 525 Anopheles sp. larvae. which were divided into 7 groups and 3 replicates, which contained 25 Anopheles spper larvae. Except for the control, each group was given clove leaf infusion starting from a concentration of 0.50%, 1%, 2%, 4% and 6%, where the treatment was carried out for 24 hours. The results showed that there was a larvicidal effect of brewing clove leaves (Syzygium aromaticum L.) on the mortality of Anopheles sp mosquito larvae, where the effective concentration was 6% and had an LC50 value in killing Anopheles sp larvae of 0.89%. The overall phytochemical content of clove leaves which has a fast response to the mortality of Anopheles sp. are saponins, flavonoids and tannins which are capable of acting as larvicides through the mechanism of damaging cell membranes or interfering with larval metabolic processes. The content of saponins, flavonoids and tannins can increase the mortality of Aedes aegypti L. larvae through the mechanism of damaging cell membranes or interfering with larval metabolic processes as stomach poisoning.
Co-Authors A.S. Satyaningtijas Amos Killay Apituley, Edwin T Arief Boediono Baszary, Chomsa Dintasari Umi Doviana Siauta Efraim Samson, Efraim Far Far, Thessa N. Hulkiawar, Welhel Y. Idris, Kurniawati Kiriweno, Grasia Welma Kubela, Dormatina La Eddy Lakesubun, Bella Frida Laratmase, Maria Laratmase, Nia D. LAURY CHARA HUWAE Lesbatta, Kezia Josawel Lesirolo, Medlin Lumingkewas, Adeleyda M. W. Martha Kaihena Mataheru, Jeanette Silvia Matakupan, Maya M. Mitha Olivia Tjiang Moniharapon, Debby Moniharapon, Debby D Moniharapon, Debby D. Moniharapon, Debby Dijola Moniharapon, Mechiavel Muskita, Edwin Nastiti Kusumorini Nastiti Kusumorini Nia D. Laratmase Nindatu, Maria Nindatu, Maria Noya, Leny Pagaya, Joseph Pattinama, Etty Rahandity, Jeanny Stevani Ratulewen, Desi Ellida Reasoa, Jesmendy Safrida Safrida Sandana, Anastasia Sanita Suriani Sapulette, Fenska Violenta Sembiring, Firly H. R. Sikafir, Beatrix Belina Sikafir, Beregita Lidiana Silahooy, Charles Silahooy, Veince B Silahooy, Veince B. Silahooy, Veince Benjamin Sillehu, Eka Safitri Simatauw, Anniestasya Z Sopacua, Gracia N. Srihadi Agungpriyono Suprihatin Suprihatin Talakua, Fernanda Clara Tetelepta, Nelma Theopilus W Watuguly Tjodi, Angel Veince Benyamin Silahooy, Veince Benyamin Welmince T. Luarwan