Djoko Wahyono
Department Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta Indonesia

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Journal : Pharmacon

Validation of Analytical Method LC MS/MS for Determination Isoniazid in Rats Serum Suhendi, Andi; Rohman, Abdul; Wahyono, Djoko; Nurrochmad, Arief; Manggo, Tasya Faradillah
Pharmacon: Jurnal Farmasi Indonesia Vol 20, No 2 (2023)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v20i2.23293

Abstract

Determination of isoniazid concentration for therapeutic drug monitoring must produce valid values, so validation of the analytical method of LC-MS/MS needs to be carried out. This research aims to obtain valid method of LCMS/MS in order to determination isoniazid in rats serum. Sample preparation was carried out using the liquid-liquid extraction method using acetonitrile and methanol (2:1). The chromatography system was developed with a C18 column (ACQUITY UPLC® HSS T3 1.8 µm, 2.1 × 100 mm), the mobile phase was 70% acetonitrile with 0.1% formic acid (A), water with 0.1% formic acid (B), as well as methanol (C) with a flow rate of 0.3 mL/min, with gradient elution, detection in ESI+ mode with multiple reaction monitoring (MRM) at the transition 138 m/z → 121 m/z. The research results showed that the method was accurate with a % recovery of 96 - 113% and an RSD of 2.4 ± 0.9%. The precision value was obtained with an average RSD of 3.7 ± 2.4% for intra-day and 4.1 ± 2.3% for inter-day. Linearity showed good results with a correlation coefficient of 0.9956 in tests with 5 different concentrations. The LOQ and LOD values obtained from the linear regression equation were 0.1987 µg/mL and 0.0656 µg/mL respectively. The results showed that the LC-M/MS method was valid for determining isoniazid levels in rat serum
Co-Authors Abdul Rohman Andi Suhendi Andriana Sari Aningtyas, Tia Arief Nurrochmad Arief Rahman Hakim Bambang Sigit Riyanto Budi Raharjo Budiarti, L Endang Cecep Kusmana Dewi Latifatul Ilma Dian Oktianti Dyah, Ria Istamining Edi Prasetyo Nugroho Endang Suparniati, Endang Endang Yuniarti Endriastuti, Nialiana Endah Fauziah Fauziah Fifin Oktaviani Fita Rahmawati Genatrika, Erza Geografi, Liniati Githa Fungie Galistiani Gunarsih, Vidiya Hapsari , Indri Harpina, Feranianty Hayati, Farida Hening Pratiwi, Hening Hidayati, Rahmania I Dewa Putu Pramantara Ika Norcahyanti Ika Puspita Sari Ika Puspitasari Indri Hapsari Irin Dwi Andari, Irin Dwi Kartika Ayu Lestari Kartikarini, Victoria Dian L. Endang Budiarti, L. Endang Laksmi Maharani Manggo, Tasya Faradillah Manik, Nirmala Megawati, Sefi Mulyasih, Anita Budi Mustofa Mustofa Nanang Munif Yasin Naniek Widyaningrum Ninisita Sri Hadi Nova Hasani Furdiyanti, Nova Hasani Nurlaila ., Nurlaila Pratiwi, Daniar Purbaningsih, Sandi Purwaningsih, Avianti Eka Dewi Aditya Putri, Dianita Rifqia Putri, Hesti Nursari Rahmantika, Fitri Retno Wahyuningrum Rihan Basyiruddin Ahmad, Rihan Basyiruddin Riyanto, Bambang S. Rizka Humardewayanti Asdie Rukminingsih, Fef S, I Dewa Putu Pramantara Sampurno Sampurno Sandi, Dita Ayulia Dwi Sari, Ika P. Sari, Lana Satibi Satibi Suci Paramitasari Syahlani Sukarno, Ristantio Tambunan, Veny Tommy Pratama, Tommy Tri Murti Andayani Umboro, Recta Olivia Wijayanto, Yayat Wulandari, Denia Yuni Yasin, Nanang M. Yayi Suryo Prabandari Yayuk Dwi Rahayu, Yayuk Dwi Yundari, Yundari Yusmein Uyun ZK Abdurahman Baizal