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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sains & Teknologi Lingkungan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Kedokteran Hewan Animal Production
Rini Widayanti
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : HAYATI Journal of Biosciences

 1 
Spike Glycoprotein 1 Partial Gene Analysis of GI-19 (QX-like) Infectious Bronchitis Virus Isolated and Propagated from Breeder, Broiler, and Layer Chickens in Java Region Santoso, Ferdinand Prayogo Cahyo; Widayanti, Rini; Widyarini, Sitarina; Wibowo, Michael Haryadi
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.414-424

Abstract

This study aims to identify and characterize receptor binding sites (RBS) and antigenic sites (HVR-I and II) of the S1 gene fragment of the infectious bronchitis virus (IBV) isolated and propagated from commercial chickens in Java Region to monitor recent circulating virus. The samples in this study were the organs which indicated infectious bronchitis infection. The stages of this research consisted of making virus suspensions, isolation, and propagation, as well as molecular detection and characterization of viruses. Virus isolation and propagation were carried out on chicken embryonated eggs aged 11 days via the allantoic route. Culture confirmation was performed by RT-PCR of the S1 gene fragment, followed by sequencing and bioinformatics analysis. The 168-hour propagation was observed in both dwarfed and curled embryos of two isolates from 11 isolates detected as IBV-positive. Phylogenetic tree construction resulted in all isolates being grouped as GI-19 genotype (QX-like). Amino acid identity among QX-like strains was calculated at 87–100%. A total of 210 predicted amino acid residues were observed, including 31 substitutions and 2 deletions. Conclusions of this study were identified and characterized as GI-19 genotype (QX-like) IBV with amino acid changes on S1 fragment from breeder, broiler, and layer chickens in Java Region.
Distinct Island Lineages of Binturong (Arctictis binturong) from Indonesia and Its Conservation Implications Hardian, Andreas Bandang; Rahmawati, Irhamna Putri; Widayanti, Rini; Aninta, Sabhrina Gita
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.916-928

Abstract

Binturong (Arctictis binturong) is a threatened carnivore that inhabits the forests of South and Southeast Asia. Despite its wide range, binturong is relatively scarce across its habitat distribution and is currently under the threat of poaching and illegal trade. Captive breeding has unfortunately been conducted rather haphazardly with a lack of origin record maintained, implicating potential risks to the management such as inbreeding or genetic swamping. This study thus aims to characterise the phylogenetic relationship of Indonesian binturong within the context of Southeast Asian binturong and further probe the distinctness of lineages originating from Java, Sumatra, Indonesian Borneo, and Bangka using Cytochrome B (CytB) and Cytochrome C Oxidase Subunit 1 (CO1). Genetic distance, phylogram topology, and haplotype analysis of both encoding genes further corroborate the distinctness of Java, Borneo, and Bangka binturong from other binturong from Indochinese regions such as India, Laos, and Myanmar. Search for prospective single nucleotide polymorphism markers to discriminate island lineages consistently found that each Java, Bangka, and Bornean binturong be distinct from each other and other lineages, especially when assessed using haplotype-based clustering. Assigning binturong originated from Sumatra is nonetheless more complicated, suggesting the possibility. Our findings substantiated the much-needed systematic research of Southeast Asian binturong as ex-situ insurance population management grows in Indonesia and other parts of the world to protect the diversity of binturong lineages and their corresponding unique evolutionary history.
Co-Authors Alek Ibrahim Alhadz, Ghias Ghifari Alnita Baaka Aninta, Sabhrina Gita Ariana Ariana Aris Haryanto Aris Purwantoro Atmoko, Bayu Andri Christin Marganingsih Santosa Claude Mona Airin Fajar Budi Lestari Handayani, Niken Satuti Hardian, Andreas Bandang Herrialfian H Hery Wijayanto I Gede Suparta Budisatria Jalaluddin J Laksono Trisnantoro Michael Haryadi Wibowo Pakpahan, Suhendra Pakpahan, Suhendra Rahmawati, Irhamna Putri Santoso, Ferdinand Prayogo Cahyo Sari, Yuslikha Khaniif Anggita Sarmin Sarmin Siswoyo, Eko Sitarina Widyarini Siti Isrina Oktavia Salasia Slamet Raharjo Soedarmanto Indarjulianto Solihin, Dedi Duryadi Sri Hartati Teuku Zahrial Helmi Teuku Zahrial Helmy Tri Wahyu Pangestiningsih Trini Susmiati Tunas Artama Wasissa, Madarina Wayan Tunas Artama Yosyana, Alyaa Rifqoh Putri Yuda Heru Fibrianto Yuriadi Y