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Talitha Widiatningrum
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Journal : Life Science

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Pertumbuhan Kalus Rejasa (Elaeocarpus grandiflorus) dari Eksplan Tangkai Daun pada Kondisi Gelap Wijawati, Nur; Habibah, Noor Aini; Musafa, Fajar; Mukhtar, Khoirul; Anggraito, Y. Ulung; Widiatningrum, Talitha
Life Science Vol 8 No 1 (2019): April 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v8i1.29986

Abstract

Rejasa (Elaeocarpus grandiflorus) is a Salatiga identity plant which is now rarely found. Rejasa produces secondary metabolites that have the potential as drugs. This study tested the growth of rejasa callus in the medium with the addition of various types and concentrations of growth regulators. The independent variables used were the type and concentration of growth regulators (2,4-dichlorophenoxyacetic acid (2,4-D) with concentrations of 1.5; 2.5 and 3.5 ppm and Picloram with a concentration of 3.5; 5; and 7.5 ppm). The dependent variable in this study was the growth of callus regeneration (percentage of callus growth, time of callus formation and morphology of callus) observed for five months in dark conditions. Explants used were young petiolus and the medium used in this study was medium Murashige and Skoog (MS). The results showed the lowest percentage of callus induction was found in explants with the addition of Picloram growth regulators with a concentration of 7.5 ppm (14%). Explants maintained in the medium with the addition of Picloram with a concentration of 5 ppm resulted in the highest percentage of callus induction. The time of callus induction is in the range of 10-22 days. The explants with the addition of Picloram growth regulator substances with a concentration of 5 ppm had the best callus induction time, which was 12 days. Most of the callus formed was friable and yellowish. Based on the results of this research, the best medium for callus induction of rejasa in dark conditions was medium with the addition of 5 ppm Picloram. Rejasa (Elaeocarpus grandiflorus) adalah tanaman identitas Salatiga yang mulai jarang ditemukan. Pertumbuhan populasinya memiliki perkembangan yang lambat. Perkembangan generatif melalui perkecambahan biji terjadi dalam tingkat yang sangat rendah. Dalam kelangkaannya, rejasa memiliki khasiat sebagai tanaman obat melalui metabolit sekunder yang dihasilkan. Penelitian ini menguji pertumbuhan kalus rejasa dalam variasi jenis dan konsentrasi zat pengatur tumbuh. Variabel bebas yang digunakan adalah jenis dan konsentrasi zat pengatur tumbuh (2,4-dichlorophenoxyacetic acid (2,4-D) dengan konsentrasi 1,5; 2,5; dan 3,5 ppm serta pikloram dengan konsentrasi 3,5; 5; dan 7,5 ppm). Variabel terikat dalam penelitian ini adalah pertumbuhan kalus rejasa (persentase tumbuh kalus, waktu berkalus, dan morfologi kalus) dalam medium yang diamati selama 5 bulan. Eksplan yang digunakan adalah tangkai muda yang ditanam dalam medium agar Murashige & Skoog dengan penambahan zat pengatur tumbuh 2,4-D dan pikloram dalam berbagai konsentrasi dan dipelihara dalam kondisi gelap. Hasil penelitian menunjukkan persentase induksi kalus paling rendah terdapat pada eksplan dengan penambahan zat pengatur tumbuh pikloram dengan konsentrasi 7,5 ppm (14%). Eksplan yang dipelihara pada medium dengan penambahan pikloram konsentrasi 5 ppm memghasilkan persentasi induksi kalus tertinggi. Waktu induksi kalus berada dalam rentang 10-22 hari. Eksplan dengan penambahan zat pengatur tumbuh pikloram konsentrasi 5 ppm memiliki rerata waktu induksi kalus paling baik yaitu 12 hari. Kalus yang terbentuk dominan berwarna kekuningan dengan jenis meremah. Berdasarkan hasil penelitiaan, medium yang paling baik untuk induksi kalus dalam kondisi gelap adalah medium MS dengan penambahan zat pengatur tumbuh pikloram konsentrasi 5 ppm.
Isolasi, Karakterisasi, dan Uji Aktivitas Antibakteri dari Bakteri Endofit Daun Kelor (Moringa oleifera) Sadikin, Nadya Audina Nurkhafiya; Bintari, Siti Harnina; Widiatningrum, Talitha; Dewi, Pramesti
Life Science Vol 10 No 2 (2021): November 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i2.54441

Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. Moringa oleifera is a medicinal plant that contains secondary metabolites and has antibacterial properties. The aim of this research is to isolate and characterize endophytic bacteria from Moringa oleifera leaves, and to test the antibacterial activity against pathogenic bacteria (Escherichia coli and Bacillus subtilus). The results of the isolation of Moringa leaf endophytic bacteria (Moringa oleifera) obtained amounted to one isolate. Based on the results of the catalase test, gram staining and spore observation, moringa oleifera leaf endophytic bacteria included in the genus Bacillus. The antibacterial test begins with the production of secondary metabolites of Moringa oleifera leaf endophytic bacteria, measures the rate of bacterial growth and tests the antibacterial activity using the Kirby Bauer method with paper disks. Observational data were analyzed statistically using ANOVA and further tests with Duncan (sig <0.05). Antibacterial activity test results showed that the incubation time of endophytic bacteria affected the growth of Escherichia coli and Bacillus subtilus bacteria. The incubation time of 10 hours showed the highest inhibition zone diameter of Escherichia coli and Bacillus subtilus bacteria respectively of 7.5 mm and 1.8 mm. The formation of inhibitory zones indicates the presence of secondary metabolite compounds from Moringa leaf endophytic bacteria which have an antibacterial effect. Bakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman. Kelor (Moringa oleifera) merupakan tanaman obat yang mengandung metabolit sekunder dan berkhasiat sebagai antibakteri. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri endofit dari daun kelor, serta menguji aktivitas antibakteri terhadap bakteri patogen (Escherichia coli dan Bacillus subtilus). Hasil isolasi bakteri endofit daun kelor (Moringa oleifera) yang diperoleh berjumlah satu isolat. Berdasarkan hasil uji katalase, pewarnaan gram dan pengamatan spora, bakteri endofit daun kelor termasuk ke dalam genus Bacillus. Uji antibakteri dimulai dengan produksi metabolit sekunder bakteri endofit daun kelor, pengukuran laju pertumbuhan bakteri dan uji aktivitas antibakteri menggunakan metode Kirby bauer dengan paper disk. Data hasil pengamatan dianalisis statistik menggunakan ANOVA dan uji lanjut dengan Duncan (sig < 0.05). Hasil uji aktivitas antibakteri menunjukkan bahwa waktu inkubasi bakteri endofit berpengaruh terhadap pertumbuhan bakteri Escherichia coli dan Bacillus subtilus. Waktu inkubasi selama 10 jam menunjukan diameter zona hambat tertinggi terhadap bakteri Escherichia coli dan Bacillus subtilus berturut-turut sebesar 7.5 mm dan 1.8 mm. Terbentuknya zona hambat mengindikasikan adanya senyawa metabolit sekunder dari bakteri endofit daun kelor yang memiliki efek antibakteri.
Efektivitas Jenis dan Frekuensi Pemberian Sampah Organik terhadap Pertumbuhan dan Kualitas Biokonservasi Maggot BSF (Hermetia illucens). Dewi, Mutiarani Kartika; Widiatningrum, Talitha; Subekti, Niken; Setiati, Ning
Life Science Vol 12 No 1 (2023): May 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i1.64137

Abstract

Organic waste is the type of waste that has the largest amount in Indonesia. If not managed properly, the organic waste can cause various kinds problem. One of the technologies to overcome this is by means composting using Black Soldier Fly (BSF) larvae. BSF larvae (maggot) has a better decomposition ability compared to organisms as well as other microorganisms. The aims of this research is to determine the most effective substarte that can affect the growth of black soldier larvae and the speed of bioconversion. This research is a quantitative descriptive study with an expreslanatory design. The study uses two variables (independent and dependent). Independent variables in this study are feeding frequency and type of organic waste as substrate. The dependent variables in this study are the speed ofbioconversion and the growth of black soldier larvae. The instruments used in this study are experiment research. The primary Data was obtained from 8 different composition and kind of organic waste. The analysis used is univariate analysis of variance using the SPSS 25.0 application. Results showed that restaurant waste is the most effective waste for reduction by black soldier fly larvae because it produces larvae with the best growth and the highest bioconversion rate among other waste.
Karakter Anatomi dan Kandungan Pigmen Daun Mangga Wirasangka (Mangifera indica var. Wirasangka) Rahayu, Reny; Rahayu, Enni Suwarsi; Rudyatmi, Ely; Widiatningrum, Talitha
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.64386

Abstract

Wirasangka mango is the identity flora of Tegal Regency. The morphological characters of the wirasangka mango have been studied, but need to be supplemented with anatomical characters. The anatomical character of the plant that needs to be studied is the anatomy of the leaves. Leaves have varying tissue structures and pigment content. This study aims to describe the anatomical characters and analyze the pigment content of wirasangka mango leaves. The characteristics and pigment content of wirasangka mango leaves have never been reported. Anatomical characters of mango wirasangka leaves were determined by cross-sectional leaf preparations using non-embedding method and whole mount leaf preparations. The pigment content of mango wirasangka leaves was analyzed using a spectrophotometer. The results of the study of the anatomical characters showed that the leaves of wirasangka mango have dorsiventral and hypostomatous leaf, have a palisade ratio of 4-5 cells, stomata are anomocytic type, guard cell length is 11.45 µm and guard cell width is 4.78 µm, and has a stomatal density of 173.25 /mm2. The chlorophyll content of shoots, young and mature leaves of wirasangka mango were 9.1 mg/l, 12.28 mg/l and 47.89 mg/l. The carotenoid content of shoots, young leaves and mature leaves were 293.51 µmol/g, 371.76 µmol/g, and 1445.88 µmol/g respectively, and the anthocyanin content of shoots, young leaves and mature leaves were respectively also 1.36 mg/100g, 0.75 mg/100g, and 0.38 mg/100g. The results of the research can be used to complete the characters that need to be observed in the characterization of wirasangka mango.
Analisis Korelasi Berbagai Faktor Budidaya terhadap Produktivitas Nira Siwalan (Borassus flabellifer L.) di Kecamatan Semanding Kabupaten Tuban Wahida, Afia Firna; Widiatningrum, Talitha; Kartijono, Nugroho Edi; Irsadi, Andin
Life Science Vol 13 No 1 (2024): May 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v13i1.65326

Abstract

Palmyra (Borassus flabellifer L.) is the identity plant of Tuban Regency. The main palmyra product in Tuban Regency is the sap. In the last 5 years (2015-2020) the productivity of sap has not increased. This study aimed to determine the correlation between cultivation factors and palmyra production in the Semanding Subdistrict, Tuban Regency, Indonesia. The research was correlative research with quantitative descriptive explanatory. The independent variables were several tree ownership, nurseries, fertilization, tapping methods, and control of plant pests. The dependent variable was the productivity of the palmyra sap. The research data sources were the people in the villages of Kowang, Gedongombo, Tegalagung, and Prunggahan, with a total sample taken of 103 people. Data collection was carried out using observation and semi-structured interviews. The results were then analyzed using the multiple linear regression application SPSS 25. The results showed that all the factors in the model, through the F test, had a significant effect simultaneously. Partially using the t-test, the factors that influence the productivity of the palmyra sap are pest control, the number of trees, and tapping methods. Therefore, the result confirmed the correlation between cultivation factors and palmyra production in the Semanding Subdistrict, Tuban Regency, Indonesia.
Co-Authors Adi Franata Jaya Aditya Marianti Afdam, Delano Hafis Ai Rohaeti Amelia, Dhea Rizky Amin Retnoningsih Andin Irsadi Ari Yuniastuti Arvidhea Safira Gunawan Aryono Adhi, Aryono Asih, Tri Sri Noor Baswara, Satsya Yoga Christina Litaay Christina Litaay Christina Litaay Dalila Daud Daud, Dalila Dewi Mustikaningtyas Dewi, Mutiarani Kartika Dhea Rizky Amelia Dzulfikar, Andi Muhammad Edi Kurniawan Ely Rudyatmi, Ely Enni Suwarsi Rahayu F. Putut Martin HB Farah Fitrotun Nisa’ Febriani, Vina Fira Putri Nur Awwalia Fitri Arum Sasi Fitri Arum Sasi, Fitri Arum Gunawan, Arvidhea Safira Hadiati, Devi Ikwan Wahyudi Imronah, Imronah Indrawati, Putri Indrawati, Putri Iryani, Riris Ismalida Maftuhatus Samihah Istiani, Fahrun Istiani, Fahrun Krispinus Kedati Pukan Krispinus Kedati Pukan Lina Herlina Lina Herlina Maila Shofa Maghfiroh Martien Herna Susanti Moh. Qalfin Muhajaroh, Ririn Muhammad Abdul Jabar Muhammad Abdullah Mukhtar, Khoirul Munasari, Tri Musafa, Fajar Nadifa, Jasmine Augi Nafi'ah, Miftahun Naina Rizki Kenarni Nanik Wijayati Nasrika, Evy Niken Subekti Nikmah, Nailatun Ning Setiati Nofitasari, Maria Ulfa Noor Aini Habibah Novita Kusuma Wardani Nugrahaningsih Nugrahaningsih Nugrahaningsih WH Nugrahaningsih WH, Nugrahaningsih Nugroho Edi Kartijono Nur Faizah Nur Kusuma Dewi Nur Kusuma Dewi Nurhafizah Nurhafizah Nurhafizah Nurhafizah Parmin Parmin - Permatasari, Yoan Peryana, Riska Pramesti Dewi Prasetya, Johanes Adi Pukan, Krispinus Putri, Raras Widiasri Putri, Safriani R Susanti R Susanti R. Susanti R. Susanti, R. Susanti Rahayu, Reny Rahayu, Reny Riska Laila Mukaromah Rizka Oktafiani Rukaenah, Rina Rukaenah, Rina S Mursiti Sadikin, Nadya Audina Nurkhafiya Salsabila, Huwaida Septina, Esky Purba Septina, Esky Purba Setianingsih, Rizki Setianingsih, Rizki Siti Alimah Siti Alimah Siti Harnina Bintari Sri Ngabekti Sri Sukaesih Sri Sukamta Sucihatiningsih Dian Wisika Prajanti Sugianto - Sulaiman, Meltasari Sulistyo, Danu Sunarsih, Sarly Triyanasari, Triyanasari Triyanasari, Triyanasari Tyas Agung Pribadi Tyas Agung Pribadi, Tyas Agung Ulfah Mediaty Arief Wahida, Afia Firna Wahyuni, Desiy Ema Dwi Walandipa, Ghani Wigatantra, Faturochim Bagas Wijawati, Nur Y. Ulung Anggraito, Y. Ulung Yarman, Indah Novi Yustinus Ulung Anggraito Zakyani, Nova N