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All Journal International Journal of Electrical and Computer Engineering HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Proceeding International Conference on Global Resource Conservation The Journal of Pure and Applied Chemistry Research Majalah Obat Tradisional Science and Technology Indonesia Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences
Nashi Widodo
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Journal : Biotropika

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BRADYKININ B2 RECEPTOR GENE POLYMORPHISM ANALYSIS IN HYPERTENSIVE PATIENTS AT DR SAIFUL ANWAR HOSPITAL MALANG Johan Aloysius; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

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Abstract

Hypertension is a major health problem in the world. When blood volume is low, juxtaglomerular cells in the kidneys secrete renin and prorenin activates the blood circulation. Plasma renin converts angiotensinogen released by the liver into angiotensin I, which is then converted to angiotensin II by the enzyme angiotensin converting enzyme (ACE) located in the pulmonary vascular endothelium. Angiotensin II causes blood vessels to constrict, resulting in increased blood pressure. ACEi inhibit the formation of angiotensin I into angiotensin II, and activate Bradykinin (BK), which causes blood vessels to dilate, thereby reducing blood pressure. However, administration of ACEi periodically cause the accumulation of BK in pulmonary vessels that cause inflammation (cough) in some people who suspected mediated by Bradykinin B2 receptor (BK2R) gene. Total of the subjects in this study is 52 subjects, DNA extracted from white blood cells (leukocytes) as much as 200 mL from whole blood by using the QIAamp kit (Qiagen). Gene BK2R in humans amplified by the method of Polymerase Chain Reaction (PCR) method using the following primers F (5-GCAGAGCTCAGCTGGAGGCG-3), and R (5-CCTCCTCGGAGCCCAGAAG-3) and identified by the method of Single Strand Conformation Polymorphism (SSCP). Results of this study shows three different genotypes there are CC, CT, and TT. Subjects with the CC genotype were most susceptible to cough, and subjects with CT genotype were least susceptible.   Keywords : ACE inhibitors, B2 Receptor, Cough, SSCP
The Effect of Different Media Content on Protease Activity Bacillus subtilis Siti Nurkasanah; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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ABSTRACT Enzymes were protein molecules that synthesized cells to accelerate biochemical reactions. One of the microorganisms that produce a protease was Bacillus subtilis. B. subtilis used in this research consisted of isolate 1 and isolate 2. Methods used include thequalitative assay of protease activity through theclear zone on skim milk agar and calculation of protease activity on production media such as NB (Nutrient Broth) and TSB (Tryptic Soy Broth). Based on the results revealed that the clear zonediameter isolate 2 better than the isolate 1 after 24hours and 48 hours incubation periods. It showed both isolates having the possibility in different strains but within a species. The highest protease activity calculation obtained from TSB production media respectively  0,14 Unit /ml unit and 0.12 Unit/ml. Keywords:B. subtilis, clear zone, protease, TSB
Amplifikasi Gen COI dan 16s rRNA dari Invertebrata Laut Plakobranchus ocellatus Fitria Eka Aprilia; Aris Soewondo; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 2, No 5 (2014)
Publisher : University of Brawijaya

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ABSTRAK Studi mengenai hubungan kekerabatan, evolusi dan identifikasi spesies secara molekuler tidak terlepas dari isolasi DNA dan amplifikasi gen COI dan 16s rRNA. Keberhasilan isolasi DNA dan amplifikasi gen COI dan 16S rRNA merupakan langkah awal yang penting untuk studi mengenai hubungan kekerabatan, evolusi dan identifikasi spesies. Penilitian ini bertujuan untuk mengamplifikasi gen COI dan 16S rRNA dari invertebrata laut yang diambil dari Raja Ampat, Plakobranchus ocellatus. Sepasang primer COI universal LCO-1490 dan HCO-2198 digunakan untuk mengamplifikasi fragmen gen COI dari P.ocellatus, sedangkan amplifikasi gen 16s rRNA menggunakan sepasang primer 16Sar-L dan 16Sbr-L. Fragmen gen COI dan 16S rRNA berhasil teramplifikasi dengan panjang amplikon masing-masing sekitar 680 bp dan 450 bp.   Kata kunci: COI, gen, identifikasi, Plakobranchus ocellatus dan 16S rRNA ABSTRACT The first important step to study about organism relationship, evolution and species identification are DNA isolation and amplification of 16S rRNA and COI genes. This research aimed to amplify COI and 16S rRNA genes of marine invertebrates taken from Raja Ampat, Plakobranchus ocellatus. A pair of COI universal primer LCO-1490 and HCO-2198 was used for ampification a fragment COI gene P.ocellatus, while a fragment of the 16s rRNA gene was amplified using 16Sbr-L and 16Sar-L primer. Either  a fragment COI or 16S rRNA genes was successfully amplified, with a length amplicon about 680 bp and 450 bp, respectively. Keywords : COI, gene, identification, Plakobrancus ocellatus, and 16S rRNA
Analisis Polimorfisme Gen Human Angiotensinogen (hAGT) di Daerah Promoter -217 Pada Penderita Hipertensi Kota Malang Secara PCR-Sekuensing Siti Fatiyatur Rahmah; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 1, No 2 (2013)
Publisher : University of Brawijaya

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Hipertensi masih menjadi masalah kesehatan utama di dunia. Polimorfisme RAAS (renin-angiotensin-aldosteron system) dikatakan sebagai penentu hipertensi dan beberapa kerusakan organ target. Angiotensinogen merupakan protein awal dalam RAAS yang memacu timbulnya hipertensi. Diketahui bahwa populasi skala besar ras Amerika dan Afrika yang terkena hipertensi, terjadi polimorfisme pada daerah promoter  di titik -217 A/G. Variasi basa pada titik -217 dari guanine menjadi adenine meningkatkan laju ekspresi gen angiotensinogen. Peningkatan ini terjadi akibat dari interaksi yang kuat antara faktor transkripsi GR dan C/EBP-β dengan daerah promoter gen angiotensinogen. Tujuan dari penelitian ini yaitu mengetahui polimorfisme gen human angiotensinogen (hAGT) di daerah promoter -217 pada komunitas hipertensi di Kota Malang. Metode yang digunakan yaitu isolasi whole genome (QIAamp Isolation Whole Genome Kit) dari sampel darah pasien hipertensi di Rumah Sakit Syaiful Anwar, Polymerase Chain Reaction (PCR) dan sekuensing. Analisis yang dilakukan yaitu menggunakan software AB sequence Scanner, Bioedit dan BLAST NCBI. Setelah dilakukan analisis diketahui bahwa tidak ditemukan adanya polimorfisme di daerah promoter -217 gen hAGT pada sampel pasien, namun ditemukan polimorfisme justru dititik yang lain yaitu -6.   Kata Kunci : Hipertensi, gen hAGT, angiotensinogen, promoter -217 A/G
Analisis Single Nucleotide Polymorphism (SNP) -217 Gen Human Angiotensinogen (hAGT) pada Penderita Hipertensi di Rumah Sakit Syaiful Anwar secara PCR-Sekuensing Siti Fatiyatur Rahmah; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 1, No 4 (2013)
Publisher : University of Brawijaya

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Abstract

ABSTRAK Hipertensi masih menjadi masalah kesehatan utama di dunia. Polimorfisme RAS (Renin Angiotensin System) dikatakan sebagai penentu hipertensi dan beberapa kerusakan organ target. Angiotensinogen merupakan protein awal dalam RAS yang memicu timbulnya hipertensi. Diketahui bahwa populasi skala besar ras Amerika dan Afrika yang terkena hipertensi, terjadi polimorfisme pada daerah 5’untranslated region di titik -217 A/G. Variasi basa pada titik -217 guanine atau adenine meningkatkan laju ekspresi gen angiotensinogen. Peningkatan ini terjadi akibat dari interaksi kuat antara faktor transkripsi GR dan C/EBP-β yang berikatan dengan daerah enhanser kemudian mempengaruhi laju transkripsi RNA polimerase III di daerah promoter gen angiotensinogen. Tujuan dari penelitian ini yaitu mengetahui adanya single nucleotide polymorphism (SNP) -217 gen human Angiotensinogen (hAGT) pada penderita hipertensi di Rumah Sakit Syaiful Anwar secara PCR-Sekuensing. Metode yang digunakan yaitu isolasi whole genome (QIAamp DNA Blood Mini Kit) dari 14 sampel darah pasien hipertensi, gen hAGT di amplifikasi dengan Polymerase Chain Reaction (PCR) dan disekuensing untuk mengetahui adanya SNP        -217. Analisis yang dilakukan yaitu menggunakan software AB sequence Scanner, Bioedit dan BLAST NCBI. Hasil analisis menunjukkan bahwa tidak ditemukan adanya SNP -217 gen hAGT pada sampel pasien. Namun terdapat varian -6A pada gen tersebut yang juga mampu memicu terjadinya hipertensi. Kata Kunci : angiotensinogen, gen hAGT, hipertensi, SNP -217 A/G
Konstruksi Protein Transcription Activator-Like Effector (TALE) 1 dan 2 dengan Target Sekuen DNA strain HPV Tipe 16 dan 18 Agatha Mia Puspitasari; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

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Abstract

Kanker serviks dapat disebabkan oleh infeksi virus HPV tipe 16 dan 18 namun dapat dihindari dengan melakukan deteksi awal atau screening pada wanita. Penelitian ini bertujuan untuk mendesain protein Transcription Activator-Like Effector (TALE) sebagai detektor dari HPV 16 dan 18 dengan cara mencari sekuen target DNA HPV 16 dan 18 beserta konstruksi protein TALE tersebut. Metode yang dilakukan adalah analisa sekuen target HPV 16 dan 18 dengan software MEGA, konstruksi protein TALE pada plasmid pSB1C3, PCR, Golden Gate Cloning, dan elektroforesis. TALE yang dikonstruksi adalah 2 macam protein TALE, yaitu TALE 1 dan TALE 2, yang memiliki susunan basa DNA yang berbeda. Konstruksi TALE dilakukan dengan Golden Gate Cloning menggunakan GATE Assembly Kit. Sekuen target DNA diperoleh dari hasil alignment software MEGA yang terdiri dari 14 bp pada masing-masing TALE dengan Timin (T) pada ujung-ujung sekuen. Kemudian konstruksi TALE diperoleh dari tim Freiburg yang memiliki 6 bagian yang dapat mengkode protein TALE. Hasil yang didapatkan dari konstruksi adalah pita DNA sebesar 3kbp pada gel agarosa melalui elektroforesis.   Kata kunci: HPV 16,  HPV 18,  kloning, TALE 1, TALE 2
Antioxidant Properties and Quantification of Phenolic and Flavonoid Compounds in Alpinia purpurata (Viell.) K. Schum Ethanol Extract Djati, Muhammad Sasmito; Azerlyn, Defiona Rensia Naomi; Kusuma, Kavana Hafil; Rosyadah, Nuraini; Kamila, Fairuz Sarah; Annisa, Yuslinda; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Widodo, Nashi
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.02

Abstract

Phenolic and flavonoid compounds have been found to have positive benefits due to their antioxidant activity. Alpinia purpurata (Viell.) K. Schum, or lengkuas merah, is known to have aromatic rhizomes and is rich in active compounds such as flavonoids and phenolics. This research aimed to investigate the total phenolic and flavonoid content of A. purpurata ethanol extract and its antioxidant activity. A. purpurata was extracted using Microwave-Assisted Extraction (MAE) with 97% ethanol solvent. The ethanol extract of A. purpurata was then analyzed for the total phenolic and flavonoid content. The antioxidant activity and nitric oxide (NO) levels were determined by DPPH and NO scavenging assay, respectively. The results showed that A. purpurata ethanol extract had a high phenolic content (148.76 ± 1.03 mg GAE.g-1extract) but weak DPPH scavenging activity (IC50 of 219.06 ± 8.15 µg.mL-1), indicating that high phenolic content does not necessarily correlate with strong antioxidant properties. In contrast, the extract exhibited strong NO scavenging activity with an IC50 of 22.73 ± 7.57 µg.mL-1. Despite its high phenolic content, the weak DPPH activity indicates that total phenolic concentration alone is not always a reliable indicator of antioxidant strength. Further studies should include additional antioxidant assays to comprehensively evaluate the extract’s antioxidant potential.
Determination of Polyphenol Compounds in Clinacanthus nutans and Their Potential as Antioxidants Widodo, Nashi; Salma Wahid Marseti; Yuslinda Annisa; Fairuz Sarah Kamila; Rosyadah, Nuraini; Rifa’i, Muhaimin
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.06

Abstract

Free radicals can cause oxidative damage if they are produced in excess amounts in the body. To prevent this, the work of endogenous antioxidants needs to be supported by safe and effective exogenous antioxidants. Clinacanthus nutans is one of the sources of natural polyphenol compounds that have the potential to be used as free radical-scavenging agents. The study began with the extraction of C. nutans simplicia with microwave assisted extraction (MAE) using a 96% ethanol solvent. The amounts of phenols and flavonoids in the extract was then measured using the Folin-Ciocalteu and aluminium chloride techniques, and their antioxidant activity was assessed using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging assays. Based on the results of the study, it is known that C. nutans ethanolic extract contains higher flavonoids (238.29 ± 10.87 mgQE.g-1) than the total phenols (148.76 ± 1.26 mgGAE.g-1). This study also showed the potential of C. nutans extract as a free-radical scavenger with an IC50 from the NO-scavenging and DPPH assays of 28.37 ± 3.38 ppm and 117.84 ± 3.93 ppm, respectively. Despite the presence of high polyphenol content in the extract, its antioxidant activity was found to be relatively weak. Consequently, further study is necessary to ascertain the specific class of compounds responsible for its antioxidant activity and to assess its effectiveness.
Co-Authors Achmad Ridok Adi Hermansyah, Adi Agatha Mia Puspitasari Agatha Mia Puspitasari Amalia, Maulida Nisa Annisa, Yuslinda Aris Soewondo Azerlyn, Defiona Rensia Naomi Azzamuddin, Haidar Custer C. Deocaris Deocaris, Custer C. Dewi Mustikaningtyas Didik Priyandoko Didik Priyandoko Dinia Rizqi Dwijayanti Dodi Iskandar, Dodi Dwi Adi Nugroho Fairuz Sarah Kamila Fitria Eka Aprilia Grahadi, Rahmat Hafil Kusuma, Kavana Hermanto, Feri Eko Ichsan Ichsan Ika Christina, Yuyun Ikhtiarini, Nur Inggita Kusumastuty Johan Aloysius Juniardi, Muhamad Rizki Kamila, Fairuz Sarah Kaul, Sunil C. Kesuma, Suryanata Khansa, Shafanony Kholilah, Tsaniyah Nur Kusuma, Kavana Hafil Listijani Suhargo, Listijani Marhendra, Eunike Berliana Mariyah Ulfa, Siti Marlita Marlita, Marlita Masruri Masruri Mifetika Lukitasari Moch Sasmito Djati Mohammad Saifur Rohman Nafisah, Wirdatun Nia Kurniawan Nugroho, Inggil Cahyo Nur Ida Panca Nugrahini Oktavia Rahayu Adianingsih Puspitoputri, Alya H. raudatul jannah raudatul jannah Renu Wadhwa Rifa'i, Muhaimin Rifa’i, Muhaimin Rizqi Dwijayanti, Dinia Rohmah, Ilmiana Nurur Rollando, Rollando Rosyadah, Nuraini Roudlotul Jannah ROUDLOTUL JANNAH Salma Wahid Marseti Sapti Puspitarini Siti Fatiyatur Rahmah Siti Fatiyatur Rahmah Siti Mariyah Ulfa Siti Nurkasanah Sri Widyarti Sri Winarsih Sunil C. Kaul Sutiman B. Sumitro Ulinnuha, Maria VALENTINA YURINA Wadhwa, Renu Warsito Warsito Wayan Firdaus Mahmudy Widekdo, Dwi purno Widyananda, Muhammad Hermawan Yanma, Nadifa Almira YOGA DWI JATMIKO Yuslinda Annisa Yuyun Ika Christina