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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology ENVIAGRO Jurnal Sumberdaya Hayati Biopropal Industri BERITA BIOLOGI Jurnal Penelitian Karet ANNALES BOGORIENSES Makara Journal of Science Annales Bogorienses
UTUT WIDYASTUTI
Institut Pertanian Bogor, Jl. Raya Dramaga, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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Journal : Makara Journal of Science

 1 
Transformation of Inhibitor of Meristem Activity (IMA) Gene into Jatropha curcas L. Paserang, Asri Pirade; Tjahjoleksono, Aris; Widyastuti, Utut; Suharsono,
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

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Jatropha is one of the many biodiesel plants developed in tropical countries. Efforts to increase its productivity can be done using various methods of breeding. One of the breeding methods is the introduction of genes into the Jatropha plant. The aim of this study is to assess the success of genetic transformation using the Inhibitor of Meristem Activity (IMA) gene in Jatropha curcas. The research procedures included inoculation of explants with Agrobacterium tumefaciens, callus induction, screening test of selection media, regeneration, and gene expression analysis using Polymerase Chain Reaction (PCR). IMA is one of the genes that controls flowering genes and ovule development. It was first isolated from tomato plants and has been successfully overexpressed in these plants using the Cauliflower Mosaic Virus (CaMV) 35S promoter. In this experiment, plant transformation was performed on J. curcas as the target. Explant callus formation in both the control and treated samples was good, but shoot formation decreased dramatically in the treated explants. PCR analysis indicated that IMA genes can be inserted into J. curcas with the size of the IMA gene is 500 bp.
Molecular Markers Application for Blast Resistance Selection on the Double Haploid Rice Population Windarsih, Gut; Utami, Dwinita Wikan; Widyastuti, Utut
Makara Journal of Science Vol. 18, No. 2
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Blast disease, caused by fungal Pyricularia grisea Sacc., is one of the most devastating diseases in rice. The use of blast-resistant rice varieties is one of the most efficient ways to control blast disease in rice. Blast-resistant varieties can be produced through breeding. The use of marker-assisted selection (MAS) available to support selection of resistant lines based on resistance gene. The objective of this research was to compare the resistance response of the double haploid lines with the differential varieties to three selected Indonesian blast races and to identify the resistance genes caused the resistance to blast based on the resistance response and the genotype evaluation using molecular markers. Forty-nine double haploid lines from a double crossing IR54/Parekaligolara//Bio110/Markuti were selected using molecular markers based on the targeted genes Pib, Pi1, Pi2, Pi9, Pi33, Pir4, and Pir7. To compare the phenotype selection, ten LTH monogenic lines of differential varieties were used. All plants tested were inoculated by three selected Indonesian blast races. The results show that the Pib gene caused a resistance to race 123, while the Pi1 and Pir7 genes caused a resistance to race 123 and 133. The Pi2, Pi9, Pi33, and Pir4 genes did not cause a resistance to race 123, 133, or 173.
ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P Yuniati, Ratna; Widyastuti, Utut; Sopandie, Didy; Yokota, Akiho; Akashi, Kinya; Suharsono, Suharsono
Makara Journal of Science Vol. 15, No. 2
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Actin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutively and is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequently used as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotide sequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of this research was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin gene sequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed from conserved region of Arabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is 610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNA sequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% with actin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to actins from Prunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet, they can be used as reference in J. curcas L. gene expression analysis.
Co-Authors . Syafarudin, . Agus Oman Sudrajat AKIHO YOKOTA ALEX HARTANA Alimuddin Andi Parenrengi Ardiwinata Ardiwinata, Ardiwinata Aris Tjahjoleksono Asep Nugraha, Asep Asri Pirade Paserang Didy Sopandie DWI SUSILANINGSIH DWINITA WIKAN UTAMI Dyah Iswantini Emma Suryati Eries Dyah Mustikarini Fadillah, Wendi Nurul Hadi Susilo Hamim Hamim Hani Susanti, Hani Ida Rosdianti, Ida Idha Susanti Indradewa, Rhian KINYA AKASHI Komar Sumantadinata Lulut Dwi Sulistyaningsih Mochamad Syaifudin, Mochamad Nampiah Sukarno Nurita Toruan-Mathius Ochieng, Fredrick Onyango PUJI LESTARI Putra, Sukma Triperdana RADITE TISTAMA Ratu Siti Aliah ROHANI CINTA BADIA GINTING Stephanie Stephanie Suharsono Suharsono Suharsono, Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Suryo Wiyono Swastika, I Nengah Tiwit Widowati, Tiwit Tri Handayani Trisnaningrum, Niken Ulfah Juniarti Siregar Umoro, Asahedi Urip Sayekti Widyaningrum, Dian Noverita windarsih, gut Yanti Rosa, Yanti Yuniati, Ratna